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All Journal Assimilation: Indonesian Journal of Biology Education Asian Journal of Agriculture
Tanjung, Aura Albizia
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Agriculture, Biological Sciences & Forestry Education

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Development of digital comic learning media based on mobile learning on sterilization and explant planting materials in plant tissue culture courses Tanjung, Aura Albizia; Harahap, Fauziyah; Khalisyah, Zhafira; Anwar, Syakila; Nazwari, Aura; Pasaribu, Fadilah; Sitompul, Naila Zahwa
Assimilation: Indonesian Journal of Biology Education Vol 8, No 2 (2025): July 2025 (Online First)
Publisher : Department of Biology Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/aijbe.v8i2.83847

Abstract

This research aims to develop a digital comic by determining the feasibility and the effectiveness of using material in the plant tissue culture to help students understand the material presented in the course at the Department of Biology, Universitas Negeri Medan. Data collection techniques include observation, giving questionnaires, and giving instruments to instructional experts, material experts, and media experts. The research method used is Research and Development (RD), which uses the ADDIE development model. This development research resulted in the creation of a digital comic learning media based on mobile learning focused on sterilization and planting materials, developed using the ADDIE (Analysis, Design, Development, Implementation, Evaluation) model.  Feasibility test results indicated high approval ratings: 93.75% from instructional experts in the “highly feasible”, 84.5% from material experts in the “highly feasible”, and 95.4% from media experts in the “highly feasible”. Lecturer responses were also favorable, with scores of 79.9. Student responses were also highly favorable, with scores of 91 from an individual group, 90.2 from a small group, and 94.2 from a limited group. Based on the data analysis, digital comic learning media based on mobile learning is effective enough for use in plant tissue culture courses.
In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal HARAHAP, FAUZIYAH; SIREGAR, ANITA RASUNA SARI; IDRAMSA, IDRAMSA; SURIANI, CICIK; EDI, SYAHMI; PANGGABEAN, NURUL HUDA; DAULAE, ABDUL HAKIM; POHAN, SELVIA DEWI; TANJUNG, AURA ALBIZIA; PERTIWI, SUFINA INTAN; KAIRANI, ANITA
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100100

Abstract

Abstract. Harahap F, Siregar ARS, Idramsa, Suriani C, Edi S, Panggabean NH, Daulae AH, Pohan SD, Tanjung AA, Pertiwi SI, Kairani A. 2026. In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal. Asian J Agric 10 (1): g100130. https://doi.org/10.13057/asianjagric/g100130. Cattleya sp. is an ornamental orchid whose seeds lack of endosperm, making natural germination and propagation difficult. In vitro culture using Protocorm-Like Bodies (PLBs) offers an alternative for mass propagation. This study aimed to evaluate the effects of coconut water and activated charcoal on the in vitro regeneration of Cattleya sp. PLBs. A completely randomized design was employed with varying concentrations of coconut water (0, 5, and 10%) and activated charcoal (0, 0.1, 0.2, and 0.3 g/L). Morphological parameters observed included time to shoot emergence, number of leaves, shoots, and roots, as well as shoot color. Data were analyzed using two-way ANOVA. The results showed that coconut water significantly accelerated shoot emergence with 10% of coconut water producing the fastest response (12 days after treatment). Activated charcoal enhanced shoot and leaf formation with 0.3 g/L, producing the highest number of shoot and leaf. Root formation was optimal at lower charcoal concentrations. Overall, the combination of coconut water and activated charcoal significantly influenced PLBS growth and regeneration. These findings indicate that appropriate combinations of organic additives can optimize in vitro regeneration of Cattleya sp. and support efficient orchid propagation.
Co-Authors Anwar, Syakila CICIK SURIANI Daulae, Abdul Hakim Fauziyah Harahap Idramsa Idramsa, Idramsa Kairani, Anita Khalisyah, Zhafira Nazwari, Aura Panggabean, Nurul Huda Pasaribu, Fadilah PERTIWI, SUFINA INTAN Selvia Dewi Pohan Siregar, Anita Rasuna Sari Sitompul, Naila Zahwa Syahmi Edi