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Development of Tissue Culture Textbook Based on Project Based-Learning to Improve Science Process Skills of Biology Department Students Universitas Negeri Medan Harahap, Salamah; Harahap, Fauziyah; Hasanah, Dwi; Sembiring, Gres Anjelia; Panggabean, Syakilah Kurnia Fahmi; Nadhilla, Annisa; Suriani, Cicik; Edi, Syahmi; Daulae, Abdul Hakim
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13786

The research on the development of this textbook was carried out based on the results of the analysis of student needs, namely that there are no teaching materials that can improve students' science process skills in the Tissue Culture course at the Department of Biology, State University of Medan. This textbook is compiled based on the learning syntax of Project-Based Learning (PjBL). This study aims to develop a Project-Based Learning-based tissue culture textbook to improve students' science process skills. This research method uses the Research and Development (R&D) with Thiagarajan's 4D (Define, Design, Develop, and Disseminate). The population of this study was students of the Biology Department of Universitas Negeri Medan with a sample of 47 people who were given a PjBL-based tissue culture textbook. The results showed that (1) the textbooks have been tested for feasibility by material experts (87,73) (2) instructional design experts (92) with a very feasible category, (3) the response of tissue culture lecturers (92) and (4) students based on individual tests (95.1), small group tests (93,75), and limited group tests (90.02) with a very good category and (5) the results of the calculation of N-Gain for science process skills were obtained at 0.66 which is in the medium category.

Perbedaan Sikap Peduli Lingkungan Siswa Berdasarkan Jenis Kelamin di SMA Percut Sei Tuan Afifah, Fadilah; Daulae, Abdul Hakim
JURNAL BIOSHELL Vol 14 No 1 (2025): April
Publisher : Universitas Islam Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/bio.v14i1.3961

Sikap peduli lingkungan tergolong salah satu aspek penting dalam pendidikan karakter yang perlu dikembangkan sejak dini, khususnya di lingkungan sekolah. Penelitian ini memfokuskan pada kaitan antara sikap peduli lingkungan dan jenis kelamin siswa, dengan tujuan untuk mengetahui apakah terdapat perbedaan signifikan. Penelitian dilakukan di SMA Negeri 2 Percut Sei Tuan pada bulan Juli 2024. Sampel penelitian 70 orang, yang terdiri dari 34 siswa yakni: laki-laki dan 36 siswa jenis kelamin perempuan. Jenis penelitian ini merupakan penelitian deskriptif kuantitatif dengan instrument angket kuisioner berjumlah 24 pernyataan. Untuk menganalisis data dari pengujian hipotesis, digunakan uji t-test dengan tingkat signifikansi (α) sebesar 0,05 dan dibantu oleh program SPSS versi 25. Hasil penelitian menunjukkan bahwa nilai rata-rata perempuan lebih tinggi daripada laki-laki. Temuan ini menunjukkan adanya perbedaan signifikan berdasarkan jenis kelamin dan pentingnya pendekatan berbasis gender dalam pendidikan lingkungan.

Analisis Kandungan Logam Berat Timbal (Pb) pada Daun Tanaman Glodokan (Polyalthia longifolia Sonn.) Menurut Kepadatan Lalu Lintas di Kawasan Tanjung Morawa Putri, Dita Aisyah; Daulae, Abdul Hakim
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 1 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i1.2209

Tanjung Morawa adalah sebuah kecamatan yang terletak di Kabupaten Deli Serdang, yang termasuk kedalam jalan lintas dengan ramai kendaraan sehingga berpotensi udara serta lingkungan sekitarnya tercemar. Penelitian ini bertujuan untuk mengetahui jumlah kandungan logam berat timbal (Pb) dan adakah hubungan antara kepadatan lalu lintas dengan kadar Timbal (Pb) pada daun glodokan (Polyathia longifolia Sonn.) di kawasan Tanjung Morawa. Pengambilan sampel dilakukan pada tiga stasiun yang telah ditentukan dengan metode pengambilan purposive random sampling masing-masing dengan pengambilan sampel tiga ulangan. Daun glodokan dianalisis di laboratorium dengan menggunakan AAS (Atomic Absorbtion Spectrophotometer). Hasil penelitian menunjukkan kadar Timbal (Pb) yang tertinggi hingga terendah secara berurutan adalah pada lokasi I sebesar 2,56 mg/kg, pada lokasi II sebesar 2,052 mg/kg, dan pada lokasi III sebesar 1,264 mg/kg. Analisis korelasi menunjukkan bahwa yang paling berpengaruh atas tingginya kadar timbal adalah kendaraan dengan roda lebih dari empat.

Development of Pocket Book of Tissue Culture Based on Case Method Harahap, Fauziyah; Sihombing, Josephine Gloria Margareth; Suriani, Cicik; Nusyirwan, Nusyirwan; Edi, Syahmi; Daulae, Abdul Hakim; Nursamsu, Nursamsu
Biosfer: Jurnal Tadris Biologi Vol 14 No 2 (2023): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v14i2.17305

The use of pocket book as a learning media is still limited. This will make it difficult for students to understand the material. The purpose of this research was to determine the feasibility results of tissue culture pocket book, the level of product feasibility through responses from lecturers, students and to see the effectiveness of tissue culture pocket book products especially in tool sterilization and in vitro propagation topics. The research method uses R&D with 4D models from Thiagrajaan. The results of the needs analysis showed that 66,7% of students had difficulty in understanding the material of tool sterilization and in vitro propagation. In curriculum analysis using the KKNI curriculum. The feasibility level of the assessment product from media experts obtained the criteria "Very worthy" with percentage of 91,67%, assessment of material experts with the criteria "worhty" with percentage of 84,74% and assessment results of learning design experts with the criteria "Very worthy" with percentage of 91,67%. Then the lecturer's response obtained results with the criteria "Worthy" with a percentage of 83,75%. In small group trials with scores of 89.4% and limited field trials of 90%. The disseminate stage obtained an N-Gain score of 0,56 with the "medium" criteria. ABSTRAK: Penggunaan buku saku sebagai media pembelajaran masih terbatas. Hal ini akan menyulitkan mahasiswa dalam memahami materi pembelajaran. Tujuan dari penelitian ini adalah untuk mengetahui hasil kelayakan buku saku kultur jaringan, tingkat kelayakan produk melalui tanggapan dari dosen, mahasiswa dan untuk melihat efektivitas produk buku saku kultur jaringan khususnya pada topik sterilisasi alat dan perbanyakan in vitro. Dalam penelitian ini menggunakan R&D dengan model 4D Thiagrajaan. Hasil analisis kebutuhan menunjukkan bahwa 66,7% mahasiswa mengalami kesulitan dalam memahami materi sterilisasi alat dan propagasi in vitro. Kurikulum yang digunakan adalah KKNI. Tingkat kelayakan produk penilaian dari ahli media diperoleh hasil dengan kriteria "Sangat layak" dengan persentase 91,67%, penilaian ahli materi dengan kriteria "Layak" dengan persentase 84,74% dan hasil penilaian ahli desain pembelajaran dengan kriteria "Sangat layak" dengan persentase 91,67%. Kemudian respon dosen diperoleh hasil dengan kriteria "Layak" dengan persentase 83,75%. Dalam uji coba kelompok kecil diperoleh skor 89,4% dan uji coba lapangan terbatas 90%. Tahap disseminate memperoleh skor N-Gain 0,56 dengan kriteria "sedang".

Kemampuan Literasi Digital pada Materi Inovasi Teknologi Biologi Siswa Arda, Gladys Mutia; Hasruddin, Hasruddin; Silitonga, Melva; Daulae, Abdul Hakim
Continuous Education: Journal of Science and Research Vol. 6 No. 2 (2025)
Publisher : Pusdikra Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51178/ce.v6i2.2739

Penelitian ini bertujuan untuk menganalisis kemampuan literasi digital pada aspek pencarian di internet, pandu arah hypertext, evaluasi konten, dan penyusunan pengetahuan siswa SMA pada mata pelajaran biologi khususnya materi inovasi teknologi biologi. Penelitian ini menggunakan metode deskriptif kuantitatif, dengan populasi penelitian yaitu seluruh siswa kelas X SMAN 1 Medan yang terdiri dari 12 kelas. Pengambilan sampel dilakukan dengan menggunakan teknik random sampling yaitu teknik undian sehingga ditetapkan 2 kelas sebagai sampel penelitian ini yaitu siswa kelas X8 dan X10 SMAN 1 Medan yang berjumlah 72 orang. Instrumen pengumpulan data adalah tes uraian untuk mendapatkan data literasi digital siswa pada aspek pencarian di internet dan pandu arah hypertext serta angket untuk mendapatkan data literasi digital siswa pada aspek evaluasi konten dan penyusunan pengetahuan. Data yang diperoleh dianalisis menggunakan analisis deskriptif dengan membuat persentase yang selanjutnya diinterpretasikan berdasarkan kategori persentase skor literasi digital siswa. Hasil penelitian ini menunjukkan bahwa kemampuan literasi digital siswa pada aspek pencarian di internet (internet searching) termasuk dalam kategori sedang (64,93%), aspek pandu arah hypertext (hypertextual navigation) termasuk dalam kategori sedang (62,78%), aspek evaluasi konten (content evaluation) termasuk dalam kategori tinggi (75,02%), aspek penyusunan pengetahuan (knowledge assembly) termasuk dalam kategori tinggi (77,22%).

CARBON DIOXIDE (CO) SEQUESTRATION IN MANGROVE LEAVES AT TANJUNG REJO VILLAGE Dawolo, Alfandi Putra Jaya; Sudibyo, Mufti; Daulae, Abdul Hakim
Riwayat: Educational Journal of History and Humanities Vol 8, No 3 (2025): July, Social Studies, Educational Research and Humanities Research.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jr.v8i3.49061

The Paluh Getah Mangrove Beach in Tanjung Rejo Village is a highly potential mangrove ecotourism site for carbon sequestration. This study aimed to assess the biomass content, carbon sequestration level, and comparative CO absorption in four mangrove leaf species (Avicennia marina, Excoecaria agallocha, Bruguiera gymnorhiza, and Ceriops tagal), as well as to analyze the effect of leaf thickness, width, and length on carbon uptake levels in Tanjung Rejo Village. Sampling was conducted at three stations determined using purposive sampling, each with three replications. Mangrove leaves were analyzed in the laboratory using the Walkley and Black method to determine carbon content. The results showed that Avicennia marina had the highest sequestration potential, followed by Bruguiera gymnorhiza, Ceriops tagal, and Excoecaria agallocha. Leaf length, width, and thickness measurements among the four species indicated variation in their CO absorption capacity.

Development of Tissue Culture Textbook Based on Project Based-Learning to Improve Science Process Skills of Biology Department Students Universitas Negeri Medan Harahap, Salamah; Harahap, Fauziyah; Hasanah, Dwi; Sembiring, Gres Anjelia; Panggabean, Syakilah Kurnia Fahmi; Nadhilla, Annisa; Suriani, Cicik; Edi, Syahmi; Daulae, Abdul Hakim
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13786

The research on the development of this textbook was carried out based on the results of the analysis of student needs, namely that there are no teaching materials that can improve students' science process skills in the Tissue Culture course at the Department of Biology, State University of Medan. This textbook is compiled based on the learning syntax of Project-Based Learning (PjBL). This study aims to develop a Project-Based Learning-based tissue culture textbook to improve students' science process skills. This research method uses the Research and Development (R&D) with Thiagarajan's 4D (Define, Design, Develop, and Disseminate). The population of this study was students of the Biology Department of Universitas Negeri Medan with a sample of 47 people who were given a PjBL-based tissue culture textbook. The results showed that (1) the textbooks have been tested for feasibility by material experts (87,73) (2) instructional design experts (92) with a very feasible category, (3) the response of tissue culture lecturers (92) and (4) students based on individual tests (95.1), small group tests (93,75), and limited group tests (90.02) with a very good category and (5) the results of the calculation of N-Gain for science process skills were obtained at 0.66 which is in the medium category.

Optimasi Sterilisasi Eksplan Biji Manggis (Garcinia mangostana L.) Menggunakan Natrium Hipoklorit Secara In vitro Harahap, Fauziyah; Asri, Elita; Hasairin, Ashar; Edi, Syahmi; Suriani, Cicik; Daulae, Abdul Hakim; Panggabean, Nurul Huda
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15281

This study evaluates the effects of NaOCl concentration and immersion time on contamination levels and viability of in vitro mangosteen seed explants, aiming to identify the optimal sterilization protocol. Conducted at YAHDI Tissue Culture Laboratory, Medan, Indonesia, a Randomized Complete Block Design tested three treatment combinations: (1) 15% and 10% NaOCl for 5 minutes, (2) 20% and 15% NaOCl for 15 minutes, and (3) 30% and 20% NaOCl for 25 minutes. After immersion, explants were rinsed three times with sterile distilled water. Observations spanned 12 MST, with data analyzed using ANOVA and Games-Howell post hoc tests; contamination sources were qualitatively described. Results showed the first combination had the lowest effectiveness, the second was better but suboptimal, while the third significantly delayed contamination and yielded the highest explant survival rate of 86.1%. The third treatment also prolonged the contamination-free period compared to others. Fungal and bacterial contaminants were predominant, highlighting the need for effective disinfectants against both. These findings indicate that the third combination is a promising sterilization protocol for mangosteen seed explants, especially for hard endocarp tissues prone to contamination.

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