Article Per Year (5 Year)
p-Index From 2021 - 2026
0.562
P-Index
This Author published in this journals
All Journal Bioscientist : Jurnal Ilmiah Biologi Jurnal MAIYAH Biosaintifika: Journal of Biology & Biology Education
Eshananda, Yuriza
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Social Sciences

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Probiotic Community of Dage Banyumas: Next Generation Sequencing Approach Eshananda, Yuriza; Fadlilah, Synta; Fikriyya, Nabela; Fitrianto, Nur; Kusumaningrum, Hermin
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22518

Abstract

Tempeh is known as a traditional fermented food that has been popular in Indonesia for hundreds of years. One of the tempeh that is widely consumed in Banyumas regency is Dage, which is made from fermented coconut waste. Currently, there is no information regarding the presence of probiotics in Dage. Therefore, this study aims to detect the diversity of probiotic types in the Dage tempeh microbiome using the Next Generation Sequencing (NGS) approach. The research stages consisted of sampling, enrichment in MRSB and R2A media, DNA extraction, Polymerase Chain Reaction (PCR), Illumina NGS-based sequencing, and bioinformatics analysis using QIIME2 and MEGA XI software. The results showed that the Dage was dominated by bacteria from the genera Lactobacillus, Weisella, and Acetobacter, which are known as a group of probiotics. In addition, bacteria from the genus Bifidobacterium and Enterococcus were also detected. Diversity analysis showed that Dage enriched in R2A medium had higher species diversity than in MRSB medium. However, the presence of the probiotic bacteria group was more abundant in MRSB medium. The dominant amplicons by phylogenetic analysis were identified as Lactobacillus spicheri, Lactobacillus zymae, Lactobacillus crustorum, Companilactobacillus farciminis, Weisella paramesenteroides, and Acetobacter indonesianensis, which previously have several probiotic properties. These results provide novel information regarding the presence of probiotic bacteria in Dage, which can be explored further using a culturable approach.
Potensi Metabolit Sekunder Tanaman Syzygium sp. Terhadap LOX-1 Sebagai Target Terapi Penyakit Aterosklerosis: Studi In Silico Raizma, Elda Zaelita Nurul; Sopian, Devia Angelina; Alifianto, Handika Maulana; Eshananda, Yuriza; Simanjuntak, Sorta Basar Ida
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14817

Abstract

Atherosclerosis is a cardiovascular disease characterized by the accumulation of oxidized lipoproteins in the arterial wall, which is triggered by LOX-1 receptor activation. The members of Syzygium genus contains secondary metabolites with antioxidant and anti-inflammatory activities that have the potential to inhibit LOX-1. This study aims to evaluate the interaction of compounds from Syzygium cumini, Syzygium aromaticum, Syzygium malaccense, and Syzygium jambos against LOX-1 using in silico method. Analysis was conducted through molecular docking, pharmacophore selection based on hydrophobic and hydrogen bond interaction criteria, and validation using Root Mean Square Deviation (RMSD ≤ 3 Å) values. The research results showed that gallic acid has the highest affinity (-4.1 kcal/mol), followed by linalool (-3.8 kcal/mol), kaempferol (-3.4 kcal/mol), oxalic acid (-3.4 kcal/mol), quercetin (-2.2 kcal/mol), and myricetin (-1.4 kcal/mol). The compounds with the highest affinity interacted with key LOX-1 residues, suggesting potential inhibition of the receptor. In conclusion, gallic acid is the most potent LOX-1 inhibitor candidate based on its stable interaction and highest affinity value. Further studies are needed to confirm its biological activity through in vitro and in vivo approaches.
Isolasi dan Uji Aktivitas Amilase pada Bakteri yang Berasal dari Hulu sungai Logawa Banyumas Al Saqaj, Adinda Salwa Amellia Putri; Saputri, Oviliana Laurentia; Septiyanti, Farah Ispramudita; Eshananda, Yuriza
MAIYAH Vol 4 No 3 (2025): Maiyah : Vol. 4 No. 3 September 2025
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2025.4.3.16805

Abstract

Amylase is an enzyme that is widely used in various industrial fields. Amylase-producing microorganisms, especially bacteria, are a potential source that continues to be explored to this day. This study aims to isolate and test amylase from the natural environment, such as the water of the Logawa River. The methods used in this study include sampling, bacterial isolation, purification, amylolytic activity testing, micromorphological and macromorphological characterization, and catalase testing on bacterial isolates. The results of the study obtained three selected bacterial isolates based on macromorphological characteristics. Amylolytic activity testing with 1% substrate showed that two isolates, namely HL 1-2 and HL1-3, had amylolytic indices of 0.97 and 1.38, respectively, which were indicated by the formation of a clear zone around the colony after 48 hours of incubation at 28 °C. All isolates are Gram-positive bacteria with positive catalase test results. The results of the study indicate that the Logawa River, especially in the upstream part, has the potential to be a source of amylolytic bacteria that can be bioprospected further.
Co-Authors Al Saqaj, Adinda Salwa Amellia Putri Alifianto, Handika Maulana Fadlilah, Synta Fikriyya, Nabela Fitrianto, Nur Kusumaningrum, Hermin Raizma, Elda Zaelita Nurul Saputri, Oviliana Laurentia Septiyanti, Farah Ispramudita Sopian, Devia Angelina Sorta Basar Ida Simanjuntak