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All Journal Journal of Fundamental and Applied Pharmaceutical Science Pharmaceutical And Biomedical Sciences Journal (PBSJ)
Zilhadia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Analysis of the Entrapment Efficiency of Thymoquinone in Microcapsules of Black Cumin Seed Oil (Nigella sativa L.) Using High Performance Liquid Chromatography Ofa Suzanti Betha; Supandi, Supandi; Addina Syahida; Boy Reynaldi Noor; Nelly Suryani; Sabrina Dahlizar; Yuni Anggraeni; Barita J. Siregar; Zilhadia
Journal of Fundamental and Applied Pharmaceutical Science Vol. 6 No. 1 (2025): August
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/jfaps.v6i1.27545

Abstract

Thymoquinone is the main active compound found in black cumin seed oil (Nigella sativa). This study intends to analyze the efficiency of its absorption in microcapsules by the ionic gelation of cross-linked alginate and the CaCl2 method, and loading with black cumin seed oil in a concentration of 20%. Thymoquinone was used as a marker to establish quality standards for this microcapsule preparation. Analysis of thymoquinone in microcapsules was performed using a pre-optimized, high-performance liquid chromatography method. Method optimization utilized an Acclaim® Polar Advantage II (C18) column with a flow speed of 1.5 mL/min, a UV detector wavelength of 252 nm, and an injection volume of 20 μL with an isocratic system on a methanol eluent composition: water (70:30). This method revealed a good linearity value (r = 0.9997) in the range of 0.5 – 500 μg/ml. The detection and quantitation limits were 8.67 μg/mL and 28.9 μg/mL, (%diff) was about -1.864 to 1.562, precision (% RSD) was 0.052 to 0.113%, and the recovery was 98.135 to 101.563%. The results of this method validation were then applied to determine thymoquinone loading in black cumin seed oil microcapsules. The results of the absorption efficiency value of black cumin seed oil microcapsules by the ionic gelation alginate and CaCl2 method, which contained 20% oil concentration in the formula, were 81.769%.
The Effect of Gelatin and Propylene Glycol on the Penetration Rate of Asiaticoside from Centella asiatica (L.) Urb Leaf Extract Geleaf Extract Gel Dahlizar, Sabrina; Anggraeni, Yuni; Munfarikhatin, Atina; Suryani, Nelly; Betha, Ofa Suzanti; Zilhadia; Fajriah, Sofa; Septama, Abdi Wira; Handayani, Maulina; Herdini
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 7 No. 1 (2025)
Publisher : Pharmaceutical and Biomedical Sciences Journal (PBSJ)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/w7prk175

Abstract

Centella asiatica (L.) Urb. is a plant known for its potential in scar treatment due to the presence of asiaticoside, a compound capable of promoting skin cell tissue repair. However, the large molecular size of asiaticoside hinders its penetration through the skin. This study aims to evaluate the effect of gelatin and propylene glycol on the transdermal penetration of asiaticoside from a gel formulation containing Centella asiatica leaf extract. Four different formulations were tested: a control (F1, without gelatin or propylene glycol); one with 15% propylene glycol (F2); one with 4% gelatin (F3); and one with 4% gelatin and 15% propylene glycol (F4). The physical characteristics of the formulations were assessed using several parameters: organoleptic properties, pH, homogeneity, centrifugation, spreadability, viscosity, and rheological behaviour. Penetration studies were performed using a Franz Diffusion Cell with a Whatman No. 1 membrane (pre-coated with Spangler solution) for 360 minutes. The penetration data were analyzed using Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS) with a C18 BEH column (2.1 × 50 mm; 1.8 μm particle size). The results showed that each formulation exhibited distinct characteristics. The study findings indicated that both gelatin and propylene glycol, as well as their combination, significantly influenced the penetration of asiaticoside in the gel formulation. Propylene glycol, functioning as a penetration enhancer, was found to increase the penetration of asiaticoside in both gelatin-based and liquid formulations.              
Co-Authors Abdi Wira Septama Addina Syahida Barita J. Siregar Boy Reynaldi Noor Herdini Maulina Handayani Munfarikhatin, Atina Nelly Suryani Ofa Suzanti Betha Sabrina Dahlizar Sofa Fajriah Supandi suryani, nelly Yuni Anggraeni Yuni Anggraeni