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Synergistic Antibacterial Activity of Curcuma domestica Val. Extract with Tetracycline Against Multidrug-resistant Acinetobacter baumannii Nasution, Halimah Raina; Yuandani, Yuandani; Septama, Abdi Wira; Nugraha, Sony Eka; Sufitni, Sufitni; Khairunnisa, Nur Aini
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.8593

Infections caused by multidrug-resistant (MDR) bacteria are on the rise globally. MDR is facilitated by overexpression of efflux pump and permeability changes of membrane. Acinetobacter baumannii is a pathogenic germ that causes a major problem in infection, also, there has been an increase in the incidence of resistance to various antibiotics. The present study highlights the synergistic of ethanolic extract of Curcuma domestica (EECD) rhizome with tetracycline against multidrug-resistant A. baumannii (MDR-Ab). Assessment of Minimum Inhibitory Concentration (MIC) was determined by microdilution using 96-well plates. The synergistic effect of EECD and tetracycline was determined by checkerboard method. The effect of EECD and tetracycline combination was investigated by bacteriolytic activity and inhibition of efflux pump by Ethidium Bromide (EtBr) accumulation assay. EECD presented the MIC value 250 µg/mL against MDR-Ab. Fractional Inhibitory Concentration Index (FICI) value of EECD and tetracycline combination was 0.4, which showed their synergistic effect. Additionally, the combination of EECD and tetracycline could inhibit the efflux pump in MDR-Ab. This combination can also compromise cell integrity by altering membrane permeability thus lysing the bacteria cells. According to these results, EECD and tetracycline combination has synergistic effects at some sites of action, and thus could be used as a breakthrough to overcome infection problems due to MDR-Ab. Keywords: Acinetobacter baumannii, antibacterial, Curcuma domestica, multidrug-resistant, tetracycline

Synthesis, Characterization, and Antibacterial Activity of Plant-Derived Zinc Oxide Nanostructure Using Lavandula angustifolia and Phyllanthus niruri Extracts Fakhirah, Dhiya; Magfira, Tya Aisha; Hutama, Aulia Sukma; Septama, Abdi Wira; Maryani, Faiza; Krismastuti, Fransiska Sri Herwahyu
Indonesian Journal of Chemistry Vol 24, No 3 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.93293

In recent years, green synthesized nanomaterials have garnered wide interest due to its inherent features like rapidity, cost-effectiveness, and environmentally friendly technique. The green synthesis of Zinc oxide nanostructures (n-ZnO) using two kinds of plant extract, lavender (Lavandula angustifolia) and meniran (Phyllanthus niruri), were discussed and their antibacterial activities were compared. Characterization by means of X-ray diffraction (XRD), Fourier transform infrared (FTIR), and field emission-scanning electron microscopy/energy dispersive X-ray spectroscopy (FE-SEM/EDS) were used to confirm the successful formation of n-ZnO using both plant extracts. The antibacterial activity of the n-ZnO synthesized from two different plant extracts was tested against Klebsiella pneumoniae and methicillin-resistant Staphylococcus aureus (MRSA). The results show that both n-ZnO has antibacterial activity against MRSA. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for n-ZnO synthesized from meniran extract were 78 and 156 mg/mL, respectively, while MIC and MBC values for n-ZnO synthesized from lavender extract were 156 and 312 mg/mL, respectively. These results confirm that the n-ZnO prepared from meniran extract is more effective in inhibiting MRSA than the n-ZnO prepared from lavender extract. This study proves that plant-based n-ZnO has anti-microbial activities and may serve as antimicrobial therapeutics.

Antibacterial, antioxidant and total flavonoid of Adenostemma lavenia (L.) ZEBUA, NILSYA FEBRIKA; FUJIKO, MUFLIHAH; DACHI, KANNE; NERDY, NERDY; SEPTAMA, ABDI WIRA
Jurnal Natural Volume 24 Number 2, June 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i2.36396

Adenostemma lavenia (L.) is one of the medicinal plants on Nias Island that is used to treat many symptoms including cough, fever and diarrhea. Empirical data of this plant suggests that these medicinal benefits can be attributed to its antibacterial and antioxidant properties, as well as the total flavonoids contained within it, but research on this is still limited. Adenostemma lavenia (L.) was extracted with ethanol, while the antibacterial properties were measured using the disc method. Antioxidant testing was conducted with the DPPH (1,1-diphenyl-2-2picrihydrazyl) inhibition method. Total flavonoids were assessed using a modified AlCl3 method with a quercetin standard. Antibacterial test results of Staphylococcus aureus, Klebsiela pneumoniae, Propionibacterium acnes, Escherichia coli, Salmonella typhi, Bacillus cereus and Shigella sonnei with mean inhibition were 8.76 0.44, 8.13 0.72, 8.77 0.45, 6.47 0.54, 7.77 0.60, 7.33 0.44, 12.06 0.60 respectively. IC50 was 59.844 3.36 ppm. Total flavonoids were 13.566 0.021 mcg/g. The results of the antibacterial test of the ethanolic extract of Adenostemma lavenia (L.) were classified as moderate. The antioxidant activity and total flavonoids were in the high category, which can be attributed to the capture of free radicals to overcome disease.

Synergistic Effect Antibacterial Activity of The Combination of Andaliman (Zanthoxylum acanthopodium DC) Fruit Essential Oil and Erythromycin against Streptococcus mutans and Streptococcus pyogenes : Efek Sinergisme Aktivitas Antibakteri Kombinasi Minyak Atsiri Buah Andaliman (Zanthoxylum acanthopodium DC) dan Eritromisin terhadap Streptococcus mutans dan Streptococcus pyogenes Situmorang, Vania Clarissha; Rahmi, Eldiza Puji; Pradana, Dhigna Luthfiyani Citra; Septama, Abdi Wira; Muti, Annisa Farida
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 10 No. 2 (2024): (October 2024)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2024.v10.i2.16977

Background: The concept of synergism is one of the approaches taken to combat the problems related to antibiotic resistance. Objectives: The goal of this study was to determine the synergistic effect of the antibacterial activity of a combination of essential oil of Zanthoxylum acanthopodium DC fruit and erythromycin against Streptococcus mutans and Streptococcus pyogenes. Methods: The broth microdilution assay was used to determine the Minimum Inhibitory Concentration (MIC) of Z. acanthpodium DC fruit essential oil and erythromycin. The synergistic effects were assessed using the checkerboard method. Results: MIC value of Z. acanthopodium DC fruit essential oil against both bacteria was 2500 mg/mL. The combination of Z. acanthopodium DC fruit essential oil and erythromycin had a synergistic effect against S. mutans and S. pyogenes with fractional inhibitory concentration index (FICI) values of 0.375 and 0.0872, respectively. Conclusions: These results suggest that a combination of herbal plants and conventional antibiotics could be used as an alternative therapy for bacterial infections.

Review: Melastoma malabathricum Natural Compounds as Inhibitors of Resistant Bacterial Development Susanti, Febria Elvy; Efdi, Mai; Syafrizayanti; Septama, Abdi Wira
Chempublish Journal Vol. 9 No. 1 (2025): Chempublish Journal
Publisher : Department of Chemistry, Faculty of Science and Technology Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/chp.v9i1.41114

The abundance of natural medicines in nature is very capable of being used and developed in various lives both individually and in combination in the modern medical field. Various developments and treatments as well as the discovery of new concepts will be able to interact with human physiology. One plant that has the potential to be developed is Melastoma malabathricum, also known as senduduk. This plant from the Melastomataceae family is easily found in tropical Asia and has long been used as an herb in traditional Chinese medicine. Based on the data collected, various studies have shown the pharmacological bioactivity of Melastoma malabathricum which has antibacterial potential and is able to fight resistant bacteria. Chemicals in this plant, including flavonoids, terpenoids, alkaloids, and steroids have been detected and shown to be inhibitors of bacterial growth, including resistant bacteria.

The Effect of Gelatin and Propylene Glycol on the Penetration Rate of Asiaticoside from Centella asiatica (L.) Urb Leaf Extract Gel Dahlizar, Sabrina; Anggraeni, Yuni; Munfarikhatin, Atina; Suryani, Nelly; Betha, Ofa Suzanti; Zilhadia, Zilhadia; Fajriah, Sofa; Septama, Abdi Wira; Handayani, Maulina; Herdini, Herdini
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 7, No 1 (2025)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.43891

Centella asiatica (L.) Urb. is a plant known for its potential in scar treatment due to the presence of asiaticoside, a compound capable of promoting skin cell tissue repair. However, the large molecular size of asiaticoside hinders its penetration through the skin. This study aims to evaluate the effect of gelatin and propylene glycol on the transdermal penetration of asiaticoside from a gel formulation containing Centella asiatica leaf extract. Four different formulations were tested: a control (F1, without gelatin or propylene glycol); one with 15% propylene glycol (F2); one with 4% gelatin (F3); and one with 4% gelatin and 15% propylene glycol (F4). The physical characteristics of the formulations were assessed using several parameters: organoleptic properties, pH, homogeneity, centrifugation, spreadability, viscosity, and rheological behaviour. Penetration studies were performed using a Franz Diffusion Cell with a Whatman No. 1 membrane (pre-coated with Spangler solution) for 360 minutes. The penetration data were analyzed using Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS) with a C18 BEH column (2.1 × 50 mm; 1.8 μm particle size). The results showed that each formulation exhibited distinct characteristics. The study findings indicated that both gelatin and propylene glycol, as well as their combination, significantly influenced the penetration of asiaticoside in the gel formulation. Propylene glycol, functioning as a penetration enhancer, was found to increase the penetration of asiaticoside in both gelatin-based and liquid formulations.

The Effect of Gelatin and Propylene Glycol on the Penetration Rate of Asiaticoside from Centella asiatica (L.) Urb Leaf Extract Geleaf Extract Gel Dahlizar, Sabrina; Anggraeni, Yuni; Munfarikhatin, Atina; Suryani, Nelly; Betha, Ofa Suzanti; Zilhadia; Fajriah, Sofa; Septama, Abdi Wira; Handayani, Maulina; Herdini
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 7 No. 1 (2025)
Publisher : Pharmaceutical and Biomedical Sciences Journal (PBSJ)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/w7prk175

Centella asiatica (L.) Urb. is a plant known for its potential in scar treatment due to the presence of asiaticoside, a compound capable of promoting skin cell tissue repair. However, the large molecular size of asiaticoside hinders its penetration through the skin. This study aims to evaluate the effect of gelatin and propylene glycol on the transdermal penetration of asiaticoside from a gel formulation containing Centella asiatica leaf extract. Four different formulations were tested: a control (F1, without gelatin or propylene glycol); one with 15% propylene glycol (F2); one with 4% gelatin (F3); and one with 4% gelatin and 15% propylene glycol (F4). The physical characteristics of the formulations were assessed using several parameters: organoleptic properties, pH, homogeneity, centrifugation, spreadability, viscosity, and rheological behaviour. Penetration studies were performed using a Franz Diffusion Cell with a Whatman No. 1 membrane (pre-coated with Spangler solution) for 360 minutes. The penetration data were analyzed using Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS) with a C18 BEH column (2.1 × 50 mm; 1.8 μm particle size). The results showed that each formulation exhibited distinct characteristics. The study findings indicated that both gelatin and propylene glycol, as well as their combination, significantly influenced the penetration of asiaticoside in the gel formulation. Propylene glycol, functioning as a penetration enhancer, was found to increase the penetration of asiaticoside in both gelatin-based and liquid formulations.

Mapping rheumatoid arthritis susceptibility through integrative bioinformatics and genomics Medi Sushanti, Nining; Adikusuma, Wirawan; Afief, Arief Rahman; La’ah, Anita Silas; Firdayani, Firdayani; Chong, Rockie; Zakaria, Zainul Amiruddin; Purwanto, Barkah Djaka; Satria, Rahmat Dani; Khair, Riat; Septama, Abdi Wira; Irham, Lalu Muhammad
Media Farmasi: Jurnal Ilmu Farmasi Vol. 20 No. 1: March 2023
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v20i1.24912

Rheumatoid arthritis (RA) is an autoimmune disease that influences several organs and tissues, especially the synovial joints, and is associated with multiple genetic and environmental factors. Numerous databases provide information on the relationship between a specific gene and the disease pathogenesis. However, it is important to further prioritize biological risk genes for downstream development and validation. This study aims to map RA-association genetic variation using genome-wide association study (GWAS) databases and prioritize influential genes in RA pathogenesis based on functional annotations. These functional annotations include missense/nonsense mutations, cis-expression quantitative trait locus (cis-eQTL), overlap knockout mouse phenotype (KMP), protein-protein interaction (PPI), molecular pathway analysis (MPA), and primary immunodeficiency (PID). 119 genetic variants mapped had a potential high risk for RA based on functional scoring. The top eight risk genes of RA are TYK2 and IFNGR2, followed by TNFRSF1A, IL12RB1 and CD40, C5, NCF2, and IL6R. These candidate genes are potential biomarkers for RA that can aid drug discovery and disease diagnosis.

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