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All Journal Journal of Aquaculture and Fish Health Journal of Artha Biological Engineering Artha Imperium: Jurnal Pengabdian Kepada Masyarakat
Riady, Reza Muhammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Medicine & Pharmacology

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Journal : Journal of Artha Biological Engineering

 1 
Study of the Characteristics of Bacteria Isolated from the Pelus River, Banyumas Regency Nurhafid, Mohammad; Indyaswari, Marthyana; Husen, Fajar; Riady, Reza Muhammad
Journal Of Artha Biological Engineering Vol. 1 No. 1 (2023)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/6wsyrt43

Abstract

Community activities around the Pelus River cause pollution which affects the bacterial community found in the river. The aim of this research was to determine the characteristics of bacteria isolated from the Pelus river environment in Banyumas Regency. The method used in this research is an exploratory method where bacterial sampling is carried out at 3 different stations. The samples taken were then isolated, observed colony morphology, as well as biochemical and physiological characterization of the bacteria. The research results obtained 10 different isolates. A total of 10 isolates were obtained based on morphological differences. The characteristics of most of the Pelus River bacteria show variations ranging from enzymatic to environmental resistance which describes the waters of the Pelus River. Bacteria isolated from the water have characteristics that identify them as bacteria capable of degrading compounds
Rapid Detection of the Aeromonas sp. group using Conventional PCR at the Aquaculture Technology Development Center, Cangkringan, Sleman, Special Region of Yogyakarta Nurhafid, Mohammad; Astuti; Riady, Reza Muhammad; Ufianah; Pardiyanto, Eko; Ruanda, Faldo Ody
Journal Of Artha Biological Engineering Vol. 1 No. 1 (2023)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/c8cxaf15

Abstract

The catfish is a freshwater cultivated fish that can be found all over Indonesia. However, the problems that occur in general are usually attacks by pathogens from the Aeromonas sp group. This research aims to optimize the rapid detection of the Aeromonas sp group using PCR at the Aquaculture Technology Development Center, Cangkringan, Sleman, special region of Yogyakarta. The research was conducted by survey with purposive sampling. Isolation was carried out using two growth media, namely Tryptone Soy Agar (TSA) and Glutamate Starch Phenile (GSP). Colonies that had characteristics of the Aeromonas sp group were subjected to PCR amplification using specific primers for the Aeromonas sp group. The results of the amplification resulted in DNA that matched the target, namely 953bp, which showed that the sample belonged to the Aeromonas sp group. Based on this technique, the level of accuracy in detecting pathogens is higher than conventional methods.
MONITORING THE ABUNDANCE OF Vibrio sp. BACTERIA IN THE WATER OF VANAME SHRIMP (Littopanaeus vannamei) BREEDING POND AT JEPARA BRACKISH WATER AQUACULTURE CENTER Trianingtyas, Laela; Ufianah, Ufianah; Ratnaningsih, Navita Desy; Kasprijo, Kasprijo; Riady, Reza Muhammad; Rizkiyanti, Ita; Zainuddin, Muhammad
Journal Of Artha Biological Engineering Vol. 2 No. 2 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/gq6rj890

Abstract

The presence of vibrio bacteria in the breeding pond is one of the important factors that affect the activities of vaname shrimp breeding. Vibrio sp. bacteria in waters can cause losses if the number is above the threshold that should be. The high abundance of Vibrio sp. bacteria in the cultivation environment is an indicator that shows the potential for disease that can reduce the growth rate and survival of shrimp. The purpose of the study was to determine the abundance and dominance of vibrio bacteria in vaname shrimp breeding ponds using the TPC (Total Plate Count) calculation technique. The methods used include bacterial sampling, preparation of tools and materials, making bacterial culture media (NA and TCBS), dilution, bacterial inoculation, and calculating the number of bacterial colonies. The results of the calculation of bacterial abundance showed that there were 9 breeding ponds with different abundances. The highest abundance was found in pond BV1 with a total bacterial value of 9.6 x 105 CFU/ml. Meanwhile, the highest vibrio dominance was found in pond BV4 with a percentage of 43.69%.
Co-Authors Anandasari, Mira Adyla Anggraeni, Runi Astuti Ayal, Esther Lourence Brendha Cecilia, Fiorentina Natasya Alfa Dini Ryandini Fajar Husen Fitriadi, Ren Indriyani Indyaswari, Marthyana Kasprijo, Kasprijo Muhammad Zainuddin Nurhafid, Mohammad Pardiyanto, Eko Putri, Novanda Bemika Ratnaningsih, Navita Desy Rizkiyanti, Ita Ruanda, Faldo Ody Trianingtyas, Laela Ufianah Ufianah, Ufianah