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Screening of proteolytic bacteria from the intestines of Tilapia Fish (Oreochromis niloticus) in the mina padi area of ​​Panembangan Village, Cilongok. Syifa Febrianti, Naily; Kasprijo, Kasprijo; Zainuddin, Muhammad; Muhammad Riady, Reza
Journal Of Artha Biological Engineering Vol. 3 No. 1 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/pm6jjq40

Abstract

Tilapia is a commodity cultivated in the rice-fish farming area of Panembangan, Banyumas. Information related to the presence of potential bacteria in the intestines of fish cultivated in rice-fish areas is still low, especially proteolytic bacteria. The purpose of this study was to determine the proportion and activity index of proteolytic bacteria in the intestines of fish. This study used an observation method with a purposive sampling technique. Proteolytic bacteria were isolated from the intestines of tilapia in blocks A and E. The bacteria that were successfully isolated were grown on 2% skim milk agar media with an incubation time of 48 hours and observed at 24-hour intervals. Based on the results of the study, the proportion of proteolytic bacteria in the intestines of tilapia from both blocks was 28% in block A and 44% in block E, respectively. The proteolytic activity index of both blocks had an average of 0.48 for block A and 0.75 for block E. The highest proteolytic activity index was from block A, namely 0.83, and in block E 3.0. Meanwhile, the smallest proteolytic index is 0.17 block E and 0.22 block A.
Effectiveness of Minaraya Probiotics on The Growth Rate and Immune Response of Catfish (Clarias gariepinus) fitriadi, ren; Sukardi, Purnama; Muhammad Riady, reza; nurhafid, mohmammad; Heratri, Agnes; Selia Irawati, Maghfira
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/bdxbgh67

Abstract

The growing demand for catfish in the market is prompting farmers to consistently enhance their production capacity. Several obstacles are encountered during production growth, such as pathogen attacks, suboptimal growth, and low survival rates. This study aimed to determine the effectiveness of Minaraya probiotics with different doses in feed on the growth and immune response of catfish. The method used in this study was an experimental method with a completely randomized design (CRD) consisting of four treatments: control (without probiotics), and treatments P1, P2, and P3 with the addition of Minaraya probiotics at 2, 5, and 10 mL per 2.5 kg of feed, respectively, each with three replications. The parameters observed included survival rate, SGR, DGR, FCR, PER, AF, RB, and leukocyte differentiation. The results showed that the survival rate in all treatments was relatively high, ranging from 95% to 98.89%. The results also showed that the addition of Minaraya probiotics significantly (p<0.05) increased growth parameters (SGR and DGR) and feed efficiency (FCR and PER), with the best dose being in treatment 3 (P3) at a dose of 10 mL/2.5 kg of feed. On the other hand, the non-specific immune response also increased in the treatment given Minaraya probiotics, as indicated by increased phagocytic activity, respiratory burst, and proportion of lymphocytes, neutrophils, and monocytes, as well as an increase in the total number of erythrocytes after Aeromonas hydrophila infection. The application of Minaraya probiotics in a sustainable cultivation system can improve catfish productivity and health.
Penerapan Teknologi Zero Waste Concept pada Pengolahan Ikan Tuna (Thunnus) di UKM Mino Sari Desa Adisara, Kecamatan Jatilawang, Kabupaten Banyumas fitriadi, ren; Hary Tjahja Soedibya, Petrus; Palupi, Mustika; Riviani; sutanto; Muhammad Riady, reza; Nurhafid, Mohammad
Artha Imperium: Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 1 (2023): 2023
Publisher : PT. Artha Genetikalab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/mkhvkh74

Abstract

Salah satu kawasan sentra pengolahan ikan yang ada di Kabupaten Banyumas, Jawa Tengah adalah Desa Adisara Kecamatan Jatilawang. Kawasan ini merupakan kawasan pengolahan ikan yang sudah ada dari tahun 1930-an. Permasalahan yang paling banyak dialami oleh UKM Mino Sari termasuk semua kelompok pengolahan di Desa Adisara adalah limbah yang mencemari lingkungan sekitar sehingga menjadi permasalahan yang belum teratasi, mengingat produksi olahan pindang tuna yang dihasilkan sekitar 12.360 kg/bulan dengan total limbah padat yang dihasilkan sebesar 4,944 kg/bulan. Tujuan kegiatan ini adalah peningkatan kompetensi pembuatan tepung dan minyak ikan dari pengolahan limbah ikan sehingga dapat menjadi produk yang bermanfaat. Target kegiatan ini adalah Kelompok Pengolahan UKM Mino Sari. Metode kegiatan pendekatan yang dilakukan dalam kegiatan ini adalah pendekatan partisipatif (PRA/Participatory Rural Appraisal) dengan cara sosialisasi dan penyuluhan. Hasil kegiatan pengabdian bahwa khalayak mitra dapat memahami rangkaian kegiatan pelatihan dan pendampingan pengelolaan limbah ikan menjadi tepung ikan. Khalayak juga dapat mentransfer pengertahuan kepada kelompok lainnya dalam rangka pernyebaran pengetahuan pembuatan tepung limbah ikan.