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Fermentasi Kopi Robusta Dengan Lactococcus lactis subsp. Untuk Meningkatkan Kualitas Rasa Kopi Nurmalasari, Enny; Kusmiyati, Kusmiyati; Yahya, Agung Kurnia; Heratri, Agnes; Kubikazari, Sinju
REACTOR: Journal of Research on Chemistry and Engineering Vol 5, No 2: December 2024
Publisher : Politeknik ATI Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52759/reactor.v5i2.157

Abstract

Fermentation in coffee provides new modulation to the taste and sensory profile of coffee, thereby enhancing its flavor quality. Lactic Acid Bacteria (LAB) strains are used for fermentation in the wet process and have been proven to improve the overall flavor of the coffee. Therefore, in this study, coffee fermentation will be carried out using Lactococcus lactis subsp. to improve coffee flavor quality. The study begins with preparing the starter medium, starter culture, and fermentation. Subsequently, proximate analysis and a cupping test are conducted to assess the flavor profile of the coffee. The results of this study show that coffee fermentation can increase the number of LAB with longer fermentation times. Furthermore, proximate results indicate that fermented Robusta coffee contains 7.86% less fat compared to non-fermented Robusta coffee, which has 15.02% fat. The cupping test results for fermented Robusta coffee reveal a total score of 81, with the clean cup and uniformity aspects scoring 10. This total is higher than that of non-fermented Robusta coffee, thus proving that fermentation using Lactococcus lactis subsp. can improve coffee flavor quality.
EFEKTIVITAS HERBSPROBIOTIK DALAM AIR MINUM TERHADAP KONSUMSI DAN KONVERSI PAKAN, PRODUKSI TELUR DAN PROFIL DARAH PUYUH Tugiyanti, Elly; Heratri, Agnes; Dewi, Ratna Stia; Ismoyowati, Ismoyowati; Sobryan, Arief; Sanjayasari, Dyahruri; Kurniawati, Any
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 13 No 2 (2025): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2025.13.2.137-146

Abstract

Penggunaan herbsprobiotic, kombinasi senyawa bioaktif herbal (jahe, kunyit, temulawak, temu ireng, dan kencur) dan mikroorganisme probiotik (Bacillus subtilis, Bifidobacterium longum, Lactobacillus bulgaricus, dan Saccharomyces cerevisiae), diteliti sebagai alternatif pemacu pertumbuhan yang ramah lingkungan. Penelitian ini bertujuan mengevaluasi pengaruh herbsprobiotic dalam air minum terhadap profil darah puyuh sebagai indikator kesehatan fisiologis. Empat perlakuan diberikan: P0 (kontrol), P1 (0,5 ml/l), P2 (1 ml/l), dan P3 (1,5 ml/l). Peubah yang diamati adalah konsumsi pakan, konsumsi minum, bobot badan, umur pertama kali bertelur, produksi telur dan bobot telur, ketebalan kerabang, jumlah eritrosit, jumlah leukosit dan rasio heterophyl/limposit (H/L). Data yang diperoleh dianalisis menggunakan analisis ragam dan dilanjutkan dengan uji beda nyata jujur. Hasil menunjukkan herbsprobiotic berpengaruh nyata terhadap peningkatan eritrosit dan hemoglobin. Peningkatan jumlah eritrosit terjadi dari 2,72 ± 0,01 x10⁶/mm³ (P0) menjadi 2,85 ± 0,06 x10⁶/mm³ (P3), dan kadar hemoglobin meningkat dari 8,96 ± 0,04 g/dL (P0) menjadi 12,49 ± 0,02 g/dL (P3). Jumlah leukosit dan rasio heterofil/limfosit (H/L) tidak menunjukkan perbedaan nyata antar perlakuan, dengan kisaran leukosit 14,92 ± 0,94 – 16,88 ± 0,77 ribu/mm³ dan rasio H/L 0,54 ± 0,18 – 0,61 ± 0,03. Peningkatan eritropoiesis diduga terjadi melalui stimulasi sumsum tulang oleh mikroba probiotik dan senyawa aktif herbal. Dengan demikian, suplementasi herbsprobiotic melalui air minum efektif meningkatkan parameter hematologis tertentu dan mendukung kesehatan puyuh. ABSTRACT The use of herbal probiotics, a combination of bioactive herbal ingredients (ginger, turmeric, temulawak, temu ireng and kencur) and probiotic microorganisms (Bacillus subtilis, Bifidobacterium longum, Lactobacillus bulgaricus and Saccharomyces cerevisiae), was investigated as an environmentally friendly alternative for growth promotion. This study aimed to evaluate the effect of herbal probiotics in drinking water on the blood profile of quail as an indicator of physiological health. Four treatments were given: P0 (control), P1 (0.5 ml/l), P2 (1 ml/l) and P3 (1.5 ml/l). The observed variables are feed consumption, water intake, body weight, age at first laying, egg production and egg weight, shell thickness, erythrocyte count, leukocyte count, and the Heterophil/Lymphocyte ratio. The obtained data were analyzed using analysis of variance and followed by the honest significant difference test. The results showed that herbal probiotic had a significant effect (P<0.05) on the increase of erythrocytes and hemoglobin. Erythrocyte count increased from 2.72 ± 0.01 x10⁶/mm³ (P0) to 2.85 ± 0.06 x10⁶/mm³ (P3), and hemoglobin level increased from 8.96 ± 0.04 g/dL (P0) to 12.49 ± 0.02 g/dL (P3). The leukocyte count and heterophil/lymphocyte (H/L) ratio showed no significant difference between treatments (P>0.05), with a leukocyte range of 14.92 ± 0.94 - 16.88 ± 0.77 thousand/mm³ and an H/L ratio of 0.54 ± 0.18 - 0.61 ± 0.03. It is thought that the increase in erythropoiesis occurs due to the stimulation of the bone marrow by probiotic microbes and active herbal compounds. Herbal probiotic supplementation via drinking water was thus effective in improving certain hematological parameters and supporting.
Effectiveness of Minaraya Probiotics on The Growth Rate and Immune Response of Catfish (Clarias gariepinus) fitriadi, ren; Sukardi, Purnama; Muhammad Riady, reza; nurhafid, mohmammad; Heratri, Agnes; Selia Irawati, Maghfira
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/bdxbgh67

Abstract

The growing demand for catfish in the market is prompting farmers to consistently enhance their production capacity. Several obstacles are encountered during production growth, such as pathogen attacks, suboptimal growth, and low survival rates. This study aimed to determine the effectiveness of Minaraya probiotics with different doses in feed on the growth and immune response of catfish. The method used in this study was an experimental method with a completely randomized design (CRD) consisting of four treatments: control (without probiotics), and treatments P1, P2, and P3 with the addition of Minaraya probiotics at 2, 5, and 10 mL per 2.5 kg of feed, respectively, each with three replications. The parameters observed included survival rate, SGR, DGR, FCR, PER, AF, RB, and leukocyte differentiation. The results showed that the survival rate in all treatments was relatively high, ranging from 95% to 98.89%. The results also showed that the addition of Minaraya probiotics significantly (p<0.05) increased growth parameters (SGR and DGR) and feed efficiency (FCR and PER), with the best dose being in treatment 3 (P3) at a dose of 10 mL/2.5 kg of feed. On the other hand, the non-specific immune response also increased in the treatment given Minaraya probiotics, as indicated by increased phagocytic activity, respiratory burst, and proportion of lymphocytes, neutrophils, and monocytes, as well as an increase in the total number of erythrocytes after Aeromonas hydrophila infection. The application of Minaraya probiotics in a sustainable cultivation system can improve catfish productivity and health.