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All Journal Jurnal Akuakultur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pembangunan Pedesaan Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Riset Akuakultur Jurnal Pengabdian Magister Pendidikan IPA Aquacultura Indonesiana Journal of Aquaculture and Fish Health MARLIN : Marine and Fisheries Science Technology Journal Jurnal Pengabdian kepada Masyarakat Nusantara Jurnal MAIYAH Molekul: Jurnal Ilmiah Kimia Jurnal Perikanan Journal of Artha Biological Engineering

Purnama Sukardi

Fakultas Perikanan dan Ilmu Kelautan Universitas Jenderal Soedirman

Author-ID : 436594

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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MODEL HUBUNGAN INTI-PLASMA PADA INDUSTRI AKUAKULTUR TAMBAK UDANG A RELATIONSHIP MODEL OF NUCLEAR-PLASMA IN AQUACULTURE INDUSTRY OF PRAWN POND Sukardi, Purnama; Margiwiyatno, Agus ; Rosyad, Anisur; , Tobari; Santoso, Jarot; Anwar, Nurul
Pembangunan Pedesaan Vol 6, No 1 (2006)
Publisher : Pembangunan Pedesaan

Show Abstract | Download Original | Original Source | Check in Google Scholar

The relationship between nuclear and plasma in aquaculture businesses as one of several agricultural businesses were observed in two regions, i.e., Lampung and West Java. CP Bahari and Triasta Citarate Ltd. were used as a sample of this relationship. The results showed that the education level of aqua-culturist was 80% senior high school and 20% secondary school. CP Bahari (nuclear) had already fulfilled the duties to the plasma aqua-culturist. However, cooperation (Bima Utama) as a channelling agent did not work properly. There was not self-help group; the only group was a neighborhood from which the company controlled aqua-culturist. Extension from Fisheries Agency Office was not properly done. Local government autonomy resulted in too many tax regulations from which overlapping taxes had been happened. Aqua-culturist cooperation (KUD) did not work as in agreement with Central Bank of Indonesia. The information of plasma credit was not clear as well as the balance position of their money. In Triasta Citarate, the existing of KUD was just to speed up the process of money transfer to the nuclear.

Evaluation Of Local Carbon Source In The Biofloc System For Juvenile Pangasius-Pangasius Culture Using Small-Scale Plastic Pond In Central Java, Indonesia Sukardi, Purnama; Prayogo, Norman A.; Pramono, Taufik Budhi; Sudaryono, Agung; Harisman, Taufan
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

The aim of the study was to investigate the effect of different types of carbohydrates on growth, survival, feed efficiency and fish production (Pangasius pangasius), in the biofloc system with zero-water exchange. Added carbohydrates were tapioca, molasses, bran and cornstarch which were set at level of 25% of the theoretical adding quantity. A total of 6000 fish larvae used in this experiments. Complete Randomized Designed was used with four treatments and three replications. Twelve tanks used in which each tank was a cylinder tank (1814.92 L) and each tank contained 500 fish. The results showed that the concentrations of ammonia and nitrite differ significantly in the experimental tank added maize when compared to other carbohydrate sources. The nitrate levels showed that highly concentration was observed in the maize treatments compare to tapioca and molasses treatments, however it was not significantly different than that of rice bran treatments. The floc volume stabilized after about 3 weeks in the BFT tanks. Different carbon sources had resulted in different proximate composition tendencies. The highest yield obtained in molasses treatments which were highest compare to all carbohydrate treatments, whilst tapioca, rice bran, and maize treatments were not significantly different

Digestive Enzyme Activities of Osteochilus vittatus with Spirulina platensis Feed Supplementation in Biofloc System Yunida, Rizka; Sukardi, Purnama; Simanjuntak, Sorta Basar Ida
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.19108

Osteochilus vittatus is a freshwater fish that needs to be developed because it tastes savory and can be used as a biocleaning agent. Research on the effect of supplementation of Spirulina platensis in nilem fish that is maintained in a biofloc system on digestive enzyme activity has never been done before. The objectives of this study were to determine the effect of S. platensis supplementation on Osteochilus vittatus digestive enzyme activity maintained in the biofloc system and to obtain the most optimum level of S. platensis supplementation in improving the digestive enzymes activity. The study was conducted experimentally with Completely Randomized Design with four treatments and five replications. Osteochilus vittatus were fed with Spirulina platensis level of 0, 2, 4, and 6 g kg-1 for 56 days. Enzyme activity was measured on days 0 and 56 with three kinds of buffer solutions. Amylase and protease activity was analyzed using ANOVA with a confidence level of 95%. The results showed that the highest amylase activity in the liver was 4.764 ± 1.705 U mg-1 protein and proximal intestine was 2.328 ± 0.838 U mg-1 protein. The highest protease activity was found in the liver and proximal intestine with activity of 6.536 ± 0.911 U mg-1 protein and 6.207 ± 2.195 U mg-1protein. Spirulina platensis supplementation level of 6 g kg-1 feed is the most optimum level (p<0.05). Cultivation of fish with S. platensis supplementation in feed can increase enzyme activity and the fish maintenance in biofloc systems can improve water quality.

Blood Hematological and Biochemical Parameters of Osteochilus vittatus with Spirulina platensis Supplementation in Biofloc System Rijal, Muhammad Azharul; Susanto, Susanto; Simanjuntak, Sorta Basar Ida; Hernayanti, Hernayanti; Sukardi, Purnama
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.20936

Spirulina platensis is a microalgae that contains nutrients such us iron and phycocianin higher than other microalgae. Research on suplementation of S. platensis in Osteochilus vittatus cultured in biofloc system has never been done. The aim of this study was to determine the blood hematological and biochemical parameters of O. vittatus fed with S. platensis supplementation and maintained in biofloc system. The study was conducted experimentally with Completely Randomized Design, four treatments and five replications. O. vittatus were fed with S. platensis level 0, 2, 4, and 6 g.kg-1 for 56 days. Blood hematological and biochemical parameters were measured on days 0 and 56. Values of those parameters were then analyzed using ANOVA with a confidence level of 95%. The result showed that the highest red blood cells, white blood cells, and hemoglobin counts were 1.57x106ÂÂÂÂ cell.mm-3, 2.37x105 cell.mm-3, and 6.77 g.dl-1 respectivley, while the highest hematocrit value was 17.5 %. The highest total protein, albumin, and globulin in blood were 7.96 g.dL-1, 4.31 g.dL-1, and 3.79 g.dL-1 respectively, and the best for ratio A/G was 1.14. S. platensis suplementation level of 4g.kg-1 feed was the most optimum level (P<0.05). Cultivation of fish with S. platensis supplementation in feed can increase fish health indicated by the hematological and biochemical parameters of the blood and the fish maintenance in biofloc systems can improve water quality. The benefit of this research is to make a good condition for cultivation and efficiently used not only water but also feed.

Identification and Expression of cGnRH-II Gene in Three Strains Osphronemus gouramy (Soang, Jepun and Bluesafir) Rosita, Rita Eka; Syakuri, Hamdan; Nuryanto, Agus; Hilmi, Endang; Sukardi, Purnama; Prayogo, Norman Arie
Molekul Vol 18 No 2 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.2.7616

Gouramy (Osphronemus gouramy) has very high economic value and is easy to cultivate. Currently there are about six strains that have been successfully cultivated based on their reproductive ability to produce eggs, namely goose (soang, goose gouramy), jepun (japan, japonica), blue sapphire, paris, bastar (broiler) and porcelain. One of the reasons for these differences in ability is internal factors which can be seen through the identification and expression of the cGnRH gene that each of these gouramy strains have. The cGnRH gene functions in signaling the pituitary gland to secrete the hormone GtH. This study aims to identify sequences and gene expression values resulting from three strains of gouramyat different age levels. The research method used was the exploration of three gouramy strains (soang, jepun, blue sapphire) at different age levels (4 months, 8 months, 12 months), and three gouramy strains were taken for each age level. This research was conducted through several stages, namely organ preparation, isolation, sequence identification and measurement of cGnRH gene expression. Sequence data was analyzed using phylogenetic trees and gene expression was analyzed using One Way ANOVA test. The sequence results showed that the soang strain had a sequence that was more similar to the jepun strain than the blue sapphire strain, and the resulting gene expression showed that the three gouramy strains with three different age levels did not give different results.

Determinasi Resiko Keamanan Pangan Berdasarkan Kandungan Escherichia coli Pada Ikan Budi Daya Mina Padi Banyumas Syuraihanah, Khotoh; Mulandari, Kusriyah; Sukardi, Purnama; Mardiyana, Mardiyana
MAIYAH Vol 3 No 1 (2024): Maiyah : Vol. 3 No. 1 Maret 2024
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2024.3.1.11711

Mina padi is a way of raising fish between rice plants, as an interval between the two rice planting seasons or raising fish as a substitute for crops in the rice fields. Panembangan Village is one of the areas in Banyumas Regency which carries out fish farming using the mina padi system with the fish reared in this cultivation being Tilapia Fish (Oreochromis niloticus) where this fish is a superior commodity from Banyumas Regency. The purpose of this study is determine the content of E. coli bacteria and how the food safety conditions in tilapia fish (O. niloticus) from the cultivation of mina padi in Panembangan Village are based on applicable standards. The analytical method used is the MPN (Most Probable Number) method with the SNI 01-2332.1-2006 and SNI 7388:2009. The results of this study found that tilapia (O. niloticus) from mina padi cultivation contained E. coli bacteria ranging from 9.1x101 – 7.3x103 colonies/gr. The content of E. coli bacteria in the cultivation of mina padi in Panembangan Village comes from the water used in the mina padi system and has exceeded the standard (<3 APM/gr) which indicates that tilapia fish (O. niloticus) is unsafe for consumption. So based on the results of this study it is necessary to monitor the water sources used and the management of fishery products so that the fish produced by Mina Padi cultivation are suitable for consumption.

Microencapsulation of single-cell protein from various microalgae species Sukardi, Purnama; Winanto, Tjahjo; Hartoyo, ,; Pramono, Taufik Budhi; Wibowo, Eko Setyo
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The objective of the research was to evaluate nutritional values of microencapsulated diet made from single cell protein of microalgae. Complete randomized design was applied using three different types of microalgae for inclusion trials i.e. (A) Nannochloropsis sp., (B) Chlorella sp., and (C) Spirulina sp. with five replications respectively. Microencapsulated diet was produced by a modification method based on thermal cross-linking with stable temperature. Phytoplankton was cultured in sea water for which fertilized by a modification of Walne and Guillard fertilizer. The results showed that the highest value of nutrition content was Spirulina sp. and the average composition of protein, crude lipid, carbohydrate, ash, nitrogen free extract, and water content was 34.80%, 0.30%, 18.53%, 20.09%, 26.29%, and 13.32%, respectively. Organoleptically, microcapsule showed that the color of capsule was dark green and smell fresh phytoplankton. Keywords: microcapsule, single-cell protein, thermal cross-linking, microalgae, phytoplankton ABSTRAK Tujuan penelitian adalah mengevaluasi kandungan nutrisi pakan mikrokapsul protein sel tunggal (single cell protein) yang berasal dari berbagai jenis mikroalga (fitoplankton). Rancangan percobaan yang digunakan adalah rancangan acak lengkap, dengan perlakuan inklusi mikrokapsul dari jenis fitoplankton (A) Nannochloropsis sp., (B) Chlorella sp., dan (C) Spirulina sp., masing-masing diulang lima kali. Pembuatan mikrokapsul dilakukan dengan menggunakan modifikasi metode dasar thermal cross-linking, serta menerapkan teknik pengeringan suhu konstan. Proses pembuatan mikrokapsul protein diawali dengan kultur fitoplankton jenis Nannochloropsis sp., Chlorella sp., dan Spirulina sp. Kultur dilakukan di dalam laboratorium menggunakan media air laut dan modifikasi pupuk Walne dan Guillard. Hasil penelitian menunjukkan bahwa kandungan nutrisi tertinggi terdapat pada jenis mikrokapsul protein sel tunggal yang berasal dari Spirulina sp., dengan rata-rata komposisi kandungan protein 34,80%, lemak 0,30%, karbohidrat 18,53%, abu 20,09%, dan BETN 26,29%. Mikrokapsul berwarna hijau tua dan aroma fitoplankton segar. Kata kunci: mikrokapsul, protein sel tunggal, thermal cross-linking, mikroalga, fitoplankton

Hydrolysed Fish Peptide, Turmeric and Garlic Powder Supplementation to Reducing Vibrio Bacteria in Litopenaeus vannamei Setiyaningsih, Lilik; Fitriadi, Ren; Kusuma, Baruna; Anjaini, Jefri; Sukardi, Purnama
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8958

Vibriosis disease caused by Vibrio bacteria is one of the main challenges in vaname shrimp (Litopenaeus vannamei) farming, as it can cause high mortality rates and decreased productivity. This study aims to evaluate the effectiveness of a combination of natural immunostimulants in turmeric (Curcuma longa) and garlic (Allium sativum) combined with hydrolysis of fish peptides in suppressing Vibrio spp. bacterial populations in the hepatopancreas organs. The study was conducted for 30 days using a completely randomised design of four treatments and three replications. Treatment A (feed+turmeric75%+garlic25%+fish peptide hydrolysis2%), Treatment B (feed+turmeric50%+garlic50%+fish peptide hydrolysis2%), Treatment C (feed+turmeric25%+garlic75%+fish peptide hydrolysis2%), and Treatment K (feed without immunostimulant and fish peptide hydrolysis). Total Vibrio spp. bacteria were counted using the total plate count method on the hepatopancreas organ of the shrimp. The results showed that the immunostimulant combination of turmeric 75%, garlic 25%, and fish peptide hydrolysis 2% (treatment A) significantly reduced the total number of Vibrio spp. bacteria in the hepatopancreas to 2.45 ± 0.213 log CFU/ml compared to the control. The combination of turmeric and garlic immunostimulants and fish peptide hydrolysis has the potential to be an effective natural strategy in controlling vibriosis disease in shrimp farming.

Penerapan Fisheye dan Microbloom Pada Kelompok Pembudidaya Ikan Desa Babakan Saputra, Wahyu Andi; Sukardi, Purnama; septiadi, Abednego Dwi
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 6 No. 1 (2025): Jurnal Pengabdian kepada Masyarakat Nusantara Edisi Januari - Maret
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v6i1.4939

Pokdakan “Samawa Fish” didirikan pada 2023 dengan tujuan meningkatkan kesejahteraan anggota dan masyarakat Desa Babakan melalui budidaya ikan gurami. Namun, mereka menghadapi berbagai masalah seperti penurunan kualitas air, penyakit ikan, efisiensi pakan rendah, dan proses jual beli tradisional. PKM ini bertujuan untuk mengatasi masalah kualitas air dengan menerapkan teknologi bioflok, mengoptimalkan perhitungan telur ikan melalui sistem FISHEYE, serta meningkatkan efisiensi pakan menggunakan teknologi mikroenkapsulasi MICROBLOOM. 3 solusi yang diajukan untuk mengatasi permasalahan yang ditemui di mitra adalah 1) Penerapan bioflok untuk meningkatkan kualitas air dan mengurangi limbah, 2) Penggunaan sistem FISHEYE untuk perhitungan telur ikan secara otomatis, 3) Implementasi teknologi mikroenkapsulasi MICROBLOOM untuk meningkatkan penyerapan nutrisi pada pakan ikan. Implementasi teknologi FISHEYE dan MICROBLOOM diharapkan menjadi solusi inovatif bagi pembudidaya ikan, peningkatan produktivitas ikan, serta keterlibatan mahasiswa melalui MBKM dalam kegiatan pembangunan desa dan penelitian

Pemberdayaan Kelompok Masyarakat Pengawas (POKMAKWAS) melalui Pelatihan Restocking Ikan Nilem untuk Mendukung Pelestarian Ikan Endemik di Desa Negarajati, Kabupaten Cilacap Sukardi, Purnama; Anjaini, Jefri; Baruna Kusuma; Asro Nurhabib; Lilik Setiyaningsih; Frentina Murti Sujadi; Rose Dewi; Arif Yulianto
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 3 (2025): Juli-September 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i3.12309

The Nilem fish (Osteochilus hasselti), an indigenous freshwater species in Indonesia, has experienced a significant population decline due to various factors including habitat degradation from land conversion, water pollution, and overfishing. This decline threatens the stability of local aquatic ecosystems and biodiversity. To address this critical issue, a Restocking program was implemented in Negarajati Village, Cilacap Regency, specifically targeting the restoration of the Nilem fish population and the improvement of damaged aquatic ecosystems. The program adopted an integrated approach emphasizing active community involvement, particularly through the empowerment of Community Supervisory Groups (POKMAKWAS). Training provided to POKMAKWAS encompassed crucial aspects such as Nilem fish restocking techniques, water quality monitoring, sustainable natural resource management, waste management, and eco-friendly aquaculture. The methodology included local breeding and seed procurement (16,000 Nilem fish measuring 8-9 cm were stocked), periodic stocking at appropriate times and locations, continuous environmental supervision and monitoring, and robust community engagement. Academics from the Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman (FPIK UNSOED), facilitated and provided technical and scientific knowledge. The Restocking initiative aims to ensure the optimal growth and development of the stocked fish, fulfilling local fish resource needs, and supporting regional food security. The empowerment of POKMAKWAS is crucial as it fosters community understanding of endemic fish conservation rules and ecosystem sustainability, enabling them to act as effective supervisory agents against habitat destruction. This program is expected to become a routine, sustainable activity for POKMAKWAS, with continuous monitoring and evaluation by the local Fisheries Department and government support. The success of this program could lead to the expansion of stocking points and significantly increase community awareness regarding the importance of managing endemic fish and aquatic ecosystems sustainably. This collaborative effort between the community, government, and academia holds potential for positive economic impacts on local fishermen and serves as a model for endemic fish conservation in other regions.

Co-Authors , Hartoyo, , -, Andreas Eka Saputra . HERNAYANTI Abednego Dwi Septiadi Achmad Iqbal Agung Sudaryono Agus Margiwiyatno Agus Nuryanto ahmad, ananda Amron Amron, Amron Anisur Rosyad Arif Yulianto Asrul Sahri Siregar Athari, Hafizha Nur Bessho, Yasumasa Ekasanti, Anandita Eko Setyo Wibowo, Eko Setyo Emyliana Listiowati, Emyliana Endang Hilmi Fitriadi, Ren Frentina Murti Sujadi Harisam, Taufan Harisman, Taufan Haryono, Florencius Eko Dwi Heratri, Agnes Insyira, Athalla Rania Jarot Santoso Kasprijo, Kasprijo Khoerudin, Fatah Kurnia, Dhimas Ragil Kusuma, Baruna Lilik Setiyaningsih Lilik Setiyaningsih Mardika, Hanisya Putri Kania Mardiyana Mardiyana Marhaeni, Bintang Masashi Kawaichi, Masashi Muhammad Riady, Reza Mulandari, Kusriyah Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nuning Vita Hidayati Nurhabib, Asro nurhafid, mohmammad Nurul Anwar Pramono, Taufik Budi Rijal, Muhammad Azharul Rose Dewi Rosita, Rita Eka Selia Irawati, Maghfira Setyawan, Agung Cahyo Soedibya, Hary Tjahja Soedibya, Petrus Hary Tjahja Sorta Basar Ida Simanjuntak Sri Marnani Susanto Susanto Syakuri, Hamdan Syuraihanah, Khotoh Taufik Budhi Pramono Tjahjo Winanto, Tjahjo Tobari Tohap Simangunsong Trianingtyas, Laela Wahyu Andi Saputra Wibowo, Satrio Haryu Wijaya, Rudy Winanto, Tjahyo Yansah, Noprie Yohanes Harvinda Yunida, Rizka Yusup, Dandi Diana

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