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All Journal Journal of Artha Biological Engineering Artha Imperium: Jurnal Pengabdian Kepada Masyarakat
Muhammad Riady, Reza
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Medicine & Pharmacology

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Journal : journal of artha biological engineering

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Screening of proteolytic bacteria from the intestines of Tilapia Fish (Oreochromis niloticus) in the mina padi area of ​​Panembangan Village, Cilongok. Syifa Febrianti, Naily; Kasprijo, Kasprijo; Zainuddin, Muhammad; Muhammad Riady, Reza
Journal Of Artha Biological Engineering Vol. 3 No. 1 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/pm6jjq40

Abstract

Tilapia is a commodity cultivated in the rice-fish farming area of Panembangan, Banyumas. Information related to the presence of potential bacteria in the intestines of fish cultivated in rice-fish areas is still low, especially proteolytic bacteria. The purpose of this study was to determine the proportion and activity index of proteolytic bacteria in the intestines of fish. This study used an observation method with a purposive sampling technique. Proteolytic bacteria were isolated from the intestines of tilapia in blocks A and E. The bacteria that were successfully isolated were grown on 2% skim milk agar media with an incubation time of 48 hours and observed at 24-hour intervals. Based on the results of the study, the proportion of proteolytic bacteria in the intestines of tilapia from both blocks was 28% in block A and 44% in block E, respectively. The proteolytic activity index of both blocks had an average of 0.48 for block A and 0.75 for block E. The highest proteolytic activity index was from block A, namely 0.83, and in block E 3.0. Meanwhile, the smallest proteolytic index is 0.17 block E and 0.22 block A.
Effectiveness of Minaraya Probiotics on The Growth Rate and Immune Response of Catfish (Clarias gariepinus) fitriadi, ren; Sukardi, Purnama; Muhammad Riady, reza; nurhafid, mohmammad; Heratri, Agnes; Selia Irawati, Maghfira
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/bdxbgh67

Abstract

The growing demand for catfish in the market is prompting farmers to consistently enhance their production capacity. Several obstacles are encountered during production growth, such as pathogen attacks, suboptimal growth, and low survival rates. This study aimed to determine the effectiveness of Minaraya probiotics with different doses in feed on the growth and immune response of catfish. The method used in this study was an experimental method with a completely randomized design (CRD) consisting of four treatments: control (without probiotics), and treatments P1, P2, and P3 with the addition of Minaraya probiotics at 2, 5, and 10 mL per 2.5 kg of feed, respectively, each with three replications. The parameters observed included survival rate, SGR, DGR, FCR, PER, AF, RB, and leukocyte differentiation. The results showed that the survival rate in all treatments was relatively high, ranging from 95% to 98.89%. The results also showed that the addition of Minaraya probiotics significantly (p<0.05) increased growth parameters (SGR and DGR) and feed efficiency (FCR and PER), with the best dose being in treatment 3 (P3) at a dose of 10 mL/2.5 kg of feed. On the other hand, the non-specific immune response also increased in the treatment given Minaraya probiotics, as indicated by increased phagocytic activity, respiratory burst, and proportion of lymphocytes, neutrophils, and monocytes, as well as an increase in the total number of erythrocytes after Aeromonas hydrophila infection. The application of Minaraya probiotics in a sustainable cultivation system can improve catfish productivity and health.
Co-Authors Fitriadi, Ren Heratri, Agnes Kasprijo, Kasprijo Muhammad Zainuddin Nurhafid, Mohammad nurhafid, mohmammad Palupi, Mustika Purnama Sukardi Riviani Selia Irawati, Maghfira Soedibya, Petrus Hary Tjahja Sutanto Syifa Febrianti, Naily