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All Journal Jurnal Sain Veteriner Jurnal Agripet
Maulana, Tulus
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Agriculture, Biological Sciences & Forestry Veterinary

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Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.) Gustina, Sri; Hasbi, Hasbi; Sonjaya, Herry; Baco, Sudirman; Qhatimah, Husnul; Saputra, Wandi; Mutmainna, Mutmainna; Kaiin, Ekayanti Mulyawati; Maulana, Tulus
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86154

Abstract

            The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P<0.01). Motility and acrosome integrity of frozen semen were not significantly difference (P>0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity.
Kinematic Characteristics of Spermatozoa in Different Goat Breeds Using Computer Assisted Semen Analysis (CASA) Hanifi, Hirzi; Umar, Sayed; Handarini, Ristika; Maulana, Tulus
Jurnal Agripet Vol 25, No 2 (2025): Volume 25, No. 2 October 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i2.47621

Abstract

Advances in semen quality analysis technology are developing rapidly. Computer-based semen analysis technology provides a more objective assessment of spermatozoa motility by measuring various kinematic movements of spermatozoa cells. This study aimed to analyze differences in spermatozoa movement patterns using Computer-Assisted Semen Analysis (CASA) in various goat breeds. This study used frozen semen from four goat breeds: Boer, Ettawa crossbred (PE), Saanen, and Anglo-Nubian. Parameter observations were conducted on thawed frozen semen from two straw samples for each group, consisting of six replicates from each breed. The observed sperm kinetic movement characteristics were VAP, VCL, VSL, DAP, DCL, DSL, STR, LIN, WOB, ALH, and BCF. The results showed that sperm movements did not differ significantly (P0.05) in VAP, VCL, VSL, DAP, DCL, and WOB. There were significant differences in DSL, the highest in Saanen 21.993.01 m/second, the highest STR and LIN values in Boer 0.690.04% and 0.450.03%, the longest ALH Anglo Nubian 6.190.39 m, and the largest BCF Boer 25.561.87 Hz. This study concludes that differences in goat breeds affect the kinematic movement of spermatozoa as measured by CASA in the parameters of DSL, STR, LIN, ALH, and BCF.
Co-Authors Hasbi Herry Sonjaya Hirzi Hanifi, Hirzi Kaiin, Ekayanti Mulyawati Mutmainna, Mutmainna Qhatimah, Husnul Ristika Handarini Saputra, Wandi Sri Gustina Sudirman Baco, Sudirman Umar, Sayed