Article Per Year (5 Year)
p-Index From 2021 - 2026
0.888
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Fahmi, Riziq Ilham Nur
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Materials Science & Nanotechnology Other

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Potential of marine sponge Jaspis sp.‐associated bacteria as an antimicrobial producer in Enggano Island Sipriyadi Sipriyadi; Riziq Ilham Nurfahmi; Uci Cahlia; Risky Hadi Wibowo; Welly Darwis; Enny Nugraheni
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.65943

Abstract

Sponges, a group of marine multicellular animals with a porous body structure, show potential for the production of bioactive compounds. Sponge‐associated bacteria are an alternative antimicrobial producer due to their high content of bioactive compounds. This study aimed to identify the highest‐potential antimicrobial‐producing bacteria isolate associated with Jaspis sp. sponges from Enggano island. The isolated bacteria were screened for antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans using cultures, supernatants, pellets, and crude extracts. The study also conducted genetic identification to determine the identity of the isolate with the greatest potency and its closest relationship using the 16S rRNA gene. The antimicrobial activity was determined by monitoring and measuring the diameter of the formed clear zones. The results of the observations of morphological characteristics revealed nine isolates from Jaspis sp. that each consisting of 6 JABS isolates and 3 JABB isolates. Based on isolates that had antimicrobial activity, JABS6 isolates had the best antimicrobial activity, with the diameter of inhibition zones of 24.7, 8.2, 4.6, and 33.7 mm for E. coli, P. aeruginosa, S. aureus and C. albicans, respectively. The genome sequencing of the JABS6 isolate confirmed that it was identical to Bacillus thuringiensis strain USS‐CAP‐1. The study concludes that this finding shows promise for the further development of future antimicrobial agents.
The Potential of Clove Rhizospheric Bacteria to Produce Vanillin from Eugenol Nurfahmi, Riziq Ilham; Mubarik, Nisa Rachmania; Rusmana, Iman; Tambunan, Ika Roostika; Akhdiya, Alina
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.702-711

Abstract

Vanillin is one of the most important flavoring agents worldwide. Currently, consumers' awareness and concern for biovanillin production has been increasing. This study aimed to screen the potential of clove rhizospheric bacteria isolates producing vanillin through a biotransformation process of eugenol and to conduct the preliminary optimization of the biotransformation condition. Twenty-eight bacteria isolates were screened for their capability to transform eugenol into vanillin. BKL 15 isolate, which was identified as Lysinibacillus xylanilyticus, was selected as the highest vanillin producer among the isolates. The optimum molar yield of vanillin produced by the selected isolate was 4.99% (1.11 g/L) after 168 hours of biotransformation process in the medium consisting of TSB (30 g/L), eugenol (24 g/L), yeast extract (20 g/L), and concentration of casamino acid (20 g/L). Throughout the publications we have read, this is the first report of L. xylanolyticus that produces vanillin.
Bioprospeksi Senyawa Antibakteri dari Bakteri yang Berasosiasi pada Bulu Babi asal Kabupaten Kaur, Bengkulu Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Adfa, Morina; Prastya, M. Eka; Cahlia, Uci; Fahmi, Riziq Ilham Nur; Rothman, Dita Delia
Jurnal Kelautan Tropis Vol 28, No 3 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i3.29412

Abstract

The digestive system of sea urchins functions to process organic material from food into bioactive compounds. . However, the direct exploitation of bioactive compounds from sea urchins may trigger ecological imbalances. These bioactive compounds can also be produced through interactions between sea urchins and their diverse associated bacteria within the body. Therefore, alternative methods are needed to obtain bioactive compounds without disrupting the aquatic ecosystem. This study aims to explore bioactive compounds from the associated bacteria of sea urchins (Stomopneustes variolaris Lamarck and Tripneustes ventricosus Lamarck) originating from Kaur Regency, Bengkulu. A total of 30 bacterial isolates were successfully obtained using Zobell Marine Agar (ZMA) medium. Potential isolates were identified through morphological observation, Gram staining, and biochemical tests. Antibacterial activity assays showed that 12 isolates had potential as antibacterial producers based on tests against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. Two isolates, TVL 6 and TVL 11, were able to inhibit all test bacteria. Additionally, assays using pellets and supernatants showed that all 12 isolates exhibited antibacterial activity, with 5 isolates showing activity in the supernatant. Isolates TVL 6 and TVL 11 were proven effective in inhibiting all test bacteria in both pellet and supernatant forms, with the highest inhibition observed against Staphylococcus aureus. These results indicate that the associated bacteria in the digestive tract of sea urchins have potential as candidates for antibacterial compound producers.   Sistem pencernaan bulu babi berfungsi untuk mengolah bahan organik dari makanan menjadi senyawa bioaktif. Namun, eksploitasi senyawa bioaktif dari bulu babi secara langsung dapat memicu ketidakseimbangan ekosistem. Senyawa bioaktif ini juga dapat dihasilkan melalui interaksi antara bulu babi dengan bakteri asosiasi yang beragam di dalam tubuhnya. Oleh karena itu, diperlukan metode alternatif untuk memperoleh senyawa bioaktif tanpa merusak keseimbangan ekosistem perairan. Penelitian ini bertujuan untuk mengeksplorasi senyawa bioaktif dari bakteri asosiasi bulu babi (Stomopneustes variolaris Lamarck dan Tripneustes ventricosus Lamarck) asal Kabupaten Kaur, Bengkulu. Sebanyak 30 isolat bakteri berhasil diisolasi menggunakan media Zobell Marine Agar (ZMA). Identifikasi isolat potensial dilakukan melalui pengamatan morfologi, pewarnaan Gram, dan uji biokimia. Uji aktivitas antibakteri menunjukkan bahwa 12 isolat memiliki potensi sebagai penghasil antibakteri berdasarkan pengujian terhadap kultur uji Bacillus subtilis, Escherichia coli, dan Staphylococcus aureus. Dua isolat, TVL 6 dan TVL 11, mampu menghambat semua bakteri uji tersebut. Selain itu, pengujian menggunakan pelet dan supernatan menunjukkan bahwa 12 isolat mampu menghasilkan aktivitas antibakteri, dengan 5 isolat menunjukkan aktivitas pada supernatan. Isolat TVL 6 dan TVL 11 terbukti efektif menghambat semua bakteri uji baik pada pelet maupun supernatan, dengan penghambatan tertinggi terhadap Stapylococcus aureus. Hasil ini mengindikasikan bahwa bakteri asosiasi dalam saluran cerna bulu babi memiliki potensi sebagai kandidat penghasil antibakteri. 
Identifikasi dan Patogenisitas Agen Bakteri Penyebab Penyakit Busuk Batang pada Buah Naga (Hylocereus sp.) Fahmi, Riziq Ilham Nur; Cahlia, Uci; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.705

Abstract

The increasing intensification of dragon fruit (Hylocereus sp.) cultivation is followed by an increase in disease attacks that have the potential to reduce plant productivity. This study aims to isolate, identify, and test the pathogenicity of bacteria that cause dragon fruit stem rot in Pematang Donok Village, Kepahiang, Bengkulu. Bacterial isolation was carried out from disease-infected stem tissue, followed by isolation purification. Bacterial identification was carried out based on colony morphology and Gram staining, while pathogenicity and hemolysis tests were carried out on potatoes and blood agar media, respectively. The data were analyzed descriptively qualitatively through observation of colony morphological characteristics and Gram staining, as well as pathogenicity tests on potatoes and hemolysis tests on blood agar. The isolation results obtained two bacterial isolates, namely isolates BBNK1 and BBNK2 with different colony morphology characteristics. Both isolates are Gram-positive bacteria with bacillus and coccus cell shapes. Pathogenicity tests on potatoes showed that both isolates were able to cause soft rot symptoms such as tissue softening, discoloration, and mucus formation compared to the control. Hemolysis tests showed that only isolate BBNK1 showed hemolytic activity with the formation of a clear zone around the isolate. The findings of this study offer foundational data to support future research on molecular identification and the development of more environmentally friendly and sustainable disease management strategies.
Co-Authors ALINA AKHDIYA Enny Nugraheni Ika Roostika Tambunan, Ika Roostika Iman Rusmana Morina Adfa NISA RACHMANIA MUBARIK Prastya, M. Eka Risky Hadi Wibowo Rothman, Dita Delia Sipriyadi Sipriyadi Sipriyadi Uci Cahlia Welly Darwis Wibowo, Risky Hadi