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Wibowo, Risky Hadi
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EVALUATION OF VARIOUS NATURAL DIETS FOR MASS REARING OF Spodoptera frugiperda J.E SMITH (LEPIDOPTERA: NOCTUIDAE) Ginting, Sempurna; Sunardi, Tri; Sari, Chaincin Buana; Wibowo, Risky Hadi
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12143-48

Abstract

Evaluation of various natural diets for mass rearing of Spodoptera frugiperda J.E Smith (Lepidoptera: Noctuidae).Spodoptera frugiperda is one of the pests that attack corn in Indonesia. This study aimed to evaluate the most suitable dietfor rearing of S. frugiperda from various natural diets. The study was conducted in vitro. The treatments were consisted ofvariation on S. frugiperda natural diets, such as maize leaf, green mustard leaf, water spinach, sweet potato leaf, sugar caneleaf, and soybeans leaf. The observed variables were life cycle period, pupa size, and pupa weight. The results showed thatthe shortest life cycle period was on corn leaves diet (40.92 days), and the longest was on sugarcane leaves (45.01 days). Thelongest size of pupa were S. frugiperda on mustard leaves diet (12.86 mm) and corn leaves (12.56 mm), The heaviest pupaweights were observed in S. frugiperda on mustard leaves diet (0.18 mg), and corn leaves (0.16 mg). Based on the data, it couldbe concluded that corn leaves were the most suitable type of diet for the growth and development of S. frugiperda.
Aktivitas Antibakteri Membran Nanokomposit Kitosan/Nanopartikel Perak Notriawan, Doni; Nesbah, Nesbah; Ernis, Gustria; Fadhila, Muhammad Adeng; Wibowo, Risky Hadi; Pertiwi, Reza; Ilfanisari, Vinolla
ALCHEMY Vol 9, No 1 (2021): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v9i1.11146

Abstract

The aim of this study was to make and test the antibacterial activity of chitosan/silver nanoparticles nanocomposite membranes. Nanocomposite membranes were synthesized by chitosan with pluronic using acetic acid as a solvent. Silver nanoparticles were synthesized using the green synthesis method and the makasar fruit (Brucea javanica L. Merr) peel extract as a bioreductor. The silver nanoparticles were composited with a chitosan/pluronic mixture and printed on a glass plate. Nanocomposite membranes were characterized using the FTIR spectrophotometer and the scanning electron microscope (SEM). Nanocomposite membrane were tested for antibacterial activity against Escherechia coli. The UV-Vis spectra showed the formation of silver nanoparticles which were indicated by the absorption at 454 nm and the absorbance value of 0.405. Characterization using FTIR showed no new functional groups formed in the composites of chitosan and pluronic. SEM results showed the difference between the chitosan membrane and the nanocomposite membrane. The surface of nanocomposite membrane showed uneven compared to the chitosan membrane. Nanocomposite membranes have antibacterial activity to inhibit E. coli growth.   Keywords: nanocomposite, chitosan/silver nanoparticles, antibacterial Penelitian ini bertujuan untuk membuat dan menguji aktivitas antibakteri membran nanokomposit kitosan/nanopartikel perak. Membran nanokomposit dibuat dengan mensintesis kitosan dengan pluronik menggunakan pelarut asam asetat. Nanopartikel perak disintesis menggunakan metode green synthesis dengan ekstrak kulit buah makasar (Brucea javanica L. Merr) sebagai bioreduktor. Nanopartikel perak dikompositkan dengan campuran kitosan/pluronik dan dicetak di atas plat kaca. Membran nanokomposit dikarakterisasi menggunakan spektrofotometer FTIR dan scanning electron microscope (SEM). Membran nanokomposit dilakukan uji aktivitas antibakteri terhadap Escherechia coli. Spektra UV-Vis menunjukkan terbentuknya nanopartikel perak yang ditandai adanya serapan pada panjang gelombang 454 nm dan absorbansi 0,405. Karakterisasi dengan FTIR menunjukkan tidak adanya gugus fungsi baru yang terbentuk pada komposit dari kitosan dan pluronik. Hasil SEM menunjukkan adanya perbedaan antara membran kitosan dengan membran nanokomposit. Permukaan membran nanokomposit terlihat tidak rata dibandingkan membran kitosan. Membran nanokomposit memiliki aktivitas bakteri sehingga dapat mengambat pertumbuhan bakteri E. coli. Kata kunci: nanokomposit, kitosan/nanopartikel perak, antibakteri
Potency of Ketapang (Terminalia catappa L.) Leaves Methanol Extract Against Pathogenic Bacteria of Catfish (Clarias batrachus L.) Wibowo, Risky Hadi; Darwis, Welly; Sipriyadi, Sipriyadi; Wahyuni, Reza; Sari, Dhea Amelia; Silvia, Elsi; Adriansyah, Aulia; Trianda, Aldy; Setiawan, Redo
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 1: Jurnal Pembelajaran Dan Biologi Nukleus Maret 2022
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i1.2475

Abstract

Ikan lele (Clarias batrachus L.) merupakan salah satu spesies penting sebagai komoditas air tawar di Indonesia dengan produksi yang terus meningkat setiap tahunnya. Namun, ikan lele rentan terhadap beberapa infeksi bakteri, terutama bila dipelihara dalam kondisi kepadatan tinggi, salah satunya oleh bakteri patogen Bacillus cereus. Banyak sumber bahan baku lokal yang dapat dimanfaatkan sebagai pakan ikan untuk mengatasi masalah tersebut dan mengatasi penyakit yang muncul pada budidaya ikan, salah satunya adalah tanaman tradisional ketapang (Terminalia catappa L.) yang merupakan tanaman yang memiliki potensi sebagai zat antibakteri. Tujuan penelitian ini untuk mengetahui dan menguji aktivitas antibakteri ekstrak daun ketapang (T. catappa L.) terhadap bakteri patogen B. cereus dari isolat hati ikan lele (Clarias batrachus L.) penyebab penyakit pada budidaya. Metode yang digunakan yaitu maserasi dengan pelarut metanol perbandingan 1:10 yang dimaserasi 7 x 24 jam. Uji aktivitas antibakteri dengan metode cakram difusi dengan konsentrasi 45%, 52,5%, 60%, 67,5%, dan 75%. Hasil uji aktivitas antibakteri ekstrak daun ketapang mampu menghambat pertumbuhan bakteri B. cereus pada konsentrasi 45% dengan rata-rata diameter zona hambat sebesar 13,51 mm (kategori kuat). Dari hasil yang diperoleh, dapat disimpulkan bahwa ekstrak daun ketapang memiliki kemampuan dalam menghambat pertumbuhan bakteri patogen B. cereus  penyebab penyakit pada hati ikan lele (C. batrachus L.)
Screening and Identification of Mangrove Plant Sonneratia alba Sm. Endophytic Bacteria From Enggano Island, Bengkulu Province Fatimatuzzahra, Fatimatuzzahra; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Simanjuntak, Putri Hezekiel Claracia; Johan, Yar
Elkawnie: Journal of Islamic Science and Technology Vol 10, No 2 (2024)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v10i2.19120

Abstract

Abstract: The mangrove plants from Enggano Island are found in a well-preserved ecosystem. Mangroves contain secondary metabolite compounds that result from the interaction between plants and endophytic bacteria. This study aims to obtain endophytic bacterial isolates from the mangrove plant Sonneratia alba Sm. from Enggano Island and to assess bacterial diversity. Endophytic bacteria were isolated using grinding and then diluted with serial dilutions of 10-1, 10-3, and 10-5. The growing bacteria were purified using the quadrant streak method. They were then identified by observing colony morphology, Gram staining, and biochemical tests including catalase, motility, citrate, urea, and sugar fermentation tests. Bacterial identification was carried out using Bergey's Manual of Determinative Bacteriology. A total of 22 endophytic bacterial isolates were collected from the mangrove plant Sonneratia alba from Enggano Island, consisting of 4 genera: Bacillus, Marinococcus, Micrococcus, and Pseudomonas. There were 17 isolates closely related to the genus of Bacillus, 1 isolate closely related to the Marinococcus, 2 isolates closely related to the Micrococcus, and 2 isolates closely related to the Pseudomonas.Abstrak: Tumbuhan mangrove asal Pulau Enggano berada pada ekosistem yang masih terjaga. Mangrove memiliki kandungan senyawa metabolit sekunder yang merupakan hasil interaksi antara tumbuhan dan bakteri endofit. Penelitian ini bertujuan untuk memperoleh isolat bakteri endofit tumbuhan mangrove Sonneratia alba Sm. asal Pulau Enggano dan mendapatkan keanekaragaman bakteri. Isolasi bakteri endofit dilakukan dengan metode gerus, kemudian diencerkan dengan pengenceran berseri dari 10-1, 10-3, dan 10-5. Bakteri yang tumbuh dimurnikan dengan metode gores kuadran. Kemudian diidentifikasi dengan mengamati morfologi koloni, pewarnaan Gram, dan uji biokimia meliputi uji katalase, uji motilitas, uji sitrat, uji urea, uji gula-gula. Kemudian dilakukan identifikasi bakteri dengan buku Bergey’s Manual of Determinative Bacteriology. Total isolat bakteri endofit yang berhasil dikoleksi dari tumbuhan mangrove Sonneratia alba asal Pulau Enggano adalah sebanyak 22 isolat dengan keanekaragaman bakteri endofit yang terdiri dari 4 genus yaitu Bacillus, Marinococcus, Micrococcus, dan Pseudomonas. Isolat yang memiliki kedekatan dengan genus Bacilus adalah sebanyak 17, yang memiliki kedekatan genus Marinococcus hanya 1 isolat, yang memiliki kedekatan dengan genus Micrococcus adalah 2 isolat, dan 2 isolat memiliki kedekatan dengan genus Pseudomonas.
Identification of Pathogenic Bacteria on Carp Commodities (Cyprinus carpio) at Quality Control and Fishery Product Safety Agency (BKIPM) of Bengkulu Wibowo, Risky Hadi
Operations Research: International Conference Series Vol. 1 No. 2 (2020): Operations Research International Conference Series (ORICS), June 2020
Publisher : Indonesian Operations Research Association (IORA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47194/orics.v1i2.72

Abstract

Carp (Cyprinus carpio) is a type of freshwater fish that is widely cultivated. The increase in the amount of production and trade in freshwater fishery commodities both for consumption in Bengkulu will potentially increase the risk of entry and spread of pests and diseases in fish, which at the same time will be a threat that can endanger and damage the sustainability of fishery biological resources. Bacteria that infect fish can inhibit the expected production targets, which is an outbreak of pathogenic fish disease caused by bacteria. This study aims to identify pathogenic bacteria that infect Carp (C. carpio). Carp were obtained from fish traders at Panorama Market, Bengkulu City. Carp samples were selected based on clinical symptoms that were no longer healthy. Isolation of bacteria from Carp’s organs using Triptic Soy Agar (TSA) media. The isolates were screened by morphological characters and biochemical test. The results of this study showed that total of 2 bacteria were isolated. Based on biochemical tests carried out such as the Simmons Citrate test, Triple Sugar Iron Agar, Oxidative-Fermentative, Motility Indol Ornithine, Lysine Iron Agar, MR-VP, urea, catalase, oxidase, gelatin, confectionery test, and Rimmler-Shotts test, pathogenic isolates Sp 1. in the sample have a close relationship with Plesiomonas shigelloides while the pathogenic isolates Sp 2. and Sp 3. have a close relationship with Aeromonas hydrophila.
Antibacterial Activities of Pseudomonas orientalis APD 16 Isolate Sponge-Associated Aplysina sp. from Enggano Island Against Escherichia coli and Staphylococcus aureus Wibowo, Risky Hadi
Operations Research: International Conference Series Vol. 1 No. 2 (2020): Operations Research International Conference Series (ORICS), June 2020
Publisher : Indonesian Operations Research Association (IORA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47194/orics.v1i2.74

Abstract

Sponge is one of the invertebrates from the Porifera phylum. Sponge bodies have structural complexity with different cell layers. The sponge has many pores (ostium) on the surface of their body as a filter feeder. Sponge is recognized as organisms that have the potential because they can produce metabolites. Secondary metabolites produced by sponges are the result of the association of sponges with bacteria. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp. sponge is known to contain metabolites with antibacterial, antifungal and cytotoxic activity on cancer cells. This study aims to identify of potential isolate associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolate was screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD 16 isolate could inhibit Escherichia coli and Staphylococcus aureus in Vitro. APD 16 isolate was identified molecularly using of 16S rRNAgenes analysis and it genetically close with Pseudomonas orientalis.
Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro Wibowo, Risky Hadi; Mubarik, Nisa Rachmania; Rusmana, Iman; Thenawidjaya, Maggy
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.406 KB) | DOI: 10.14692/jfi.13.3.105

Abstract

Chitinolytic bacteria have been reported as biocontrol agents and have the ability to produce chitinase enzymes. The objective of the research was to obtain chitinase producing bacteria that had antagonistic activity to Ganoderma boninense, a causal agent of basal stem rot on oil palm. A total of 63 isolates of chitinase producing bacteria were isolated from soil of Bukit Dua Belas National Park and oil palm plantation in Jambi Province; all was screened for their potency in inhibiting G. boninense in vitro. Three isolates designated TB04-05, SW01-11, and SW02-08 were potentially suppressed and inhibited the mycelium growth of G. boninense in vitro. Based on their specific chitinase activity, these three isolates produced the highest level of chitinase enzyme of 6.3072 U mg-1 protein, 6.0385 U mg-1 protein and 6.1279 U mg-1 protein, respectively after 24 hr incubation. Based on 16S RNA identification, strain TB04-05 had similarity with Bacillus cereus, whereas strains SW01 and SW02-08 had similarity with Bacillus thuringiensis.
Isolation and Identification of Endophytic Bacteria from Euphorbia hirta L. (Patikan Kebo) Rindiyani, Rindiyani; Anugrah, Ratu Intan; Nabila, Bella; Salsabilla, Sisilia; Susanti, Elia; Rofiani, Desi; Puspytha, Yolanda; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Darwis, Welly
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6688

Abstract

Patikan Kebo (Euphorbia hirta L.) is a plant that lives scattered in the yard, has potential as an antibacterial, one way to extract plants is by isolating endophytic bacteria. Endophytes are bacteria found in plant tissue. The purpose of this study was to isolate and identify the morphology of endophytic bacteria from  plant Patikan Kebo (Euphorbia hirta L.). The method used is the Total Plate Count (TPC) method by taking samples, isolating bacteria, pure culture, morphological identification, and Gram staining. Endophytic bacteria were obtained by isolating parts of the plant Patikan Kebo (E. hirta L.), namely the roots of patikan kebo (PRE), Patikan kebo stem endophyte (PSE) and Patikan kebo leaves endophyte (PLE). The results of the study found 22 isolates, endophytic bacterial isolates have diverse morphological characteristics including appearance, elevation, edges and color. There are 20 isolates of endophytic bacteria that belong to the Gram positive group and 2 isolates of endophytic bacteria that belong to the Gram negative group. It can be concluded that endophytic bacterial isolates from Patikan Kebo (Euphorbia hirta L.) plants have different morphologies
Genetic Characteristics of Chloropsis cochinchinensis Gmelin, 1789 Based on The Mitochondrial DNA COI Gene Jarulis, Jarulis; Saputra, Apin; Wibowo, Risky Hadi
Jurnal Biodjati Vol 8 No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.20260

Abstract

The rate of illegal poaching of blue-winged leafbirds (Chloropsis cochinchinensis) throughout Indonesia, particularly Bengkulu, is quite high. However, only minimal molecular information is available for this species. We performed mtDNA COI gene sequencing to explore genetic characters (conservative site, variable site, parsimony site, and singleton site) of blue-winged leafbirds. Using Qiagen's DNeasy® Blood and Tissue Kit based on the Spin-Column Protocol, total DNA was isolated, and PCR amplification methods were performed. DNA derived from a PCR reaction was forwarded to PT. First Base Malaysia for sequencing. Using MEGA 10.0 and BIOEDIT, the COI gene nucleotide sequence data were assembled, edited, and analyzed to explore of single nucleotide polymorphism, genetic distance, and phylogeny. The 616 bp COI genes contained 566 conservative sites (C), 50 variation sites (V), 24 information parsimony sites (Pi), and 26 singleton sites (S), as indicated by the results. The greatest nucleotide base composition was cytosine (34.1–34.9%), while the lowest was guanine (15.7–16.2%). The proportion of adenine-thymine nucleotide base pairs was greater than that of guanine-cytosine (50.3%). There were 26 barcode-specific mutation sites, 17 transition substitution mutation sites, and 9 transverse substitution mutation sites. The average genetic distance between C. cochinchinensis individuals was 2.2%, but the average genetic difference between species was 9.0%. All C. cochinchinensis individuals in our sample clustered within the same clade and were distinguished from other species within the same genus. The COI gene sequences of C. cochinchinensis that we acquired are novel and can be utilized for molecular identification of the species.
Pemberdayaan Masyarakat Nelayan di Daerah 3T melalui Kegiatan Produksi dan Pelatihan Pemasaran Jompol (Moolgarda seheli) Presto “Ya U Waika” Sipriyadi, Sipriyadi; Hayu, Rina Suthia; Yuliza, Elfi; Wibowo, Risky Hadi; Setiawan, Redo; Wulandari, Gustina Dwi; Aini, Nurul
Jurnal Pengabdian Pada Masyarakat Vol 10 No 2 (2025): Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/jppm.v10i2.1139

Abstract

Pulau Enggano sebagai daerah 3T di Indonesia yang masuk wilayah administrasi Bengkulu Utara menyimpan berbagai potensi sumber daya alam yang sangat besar. Posisi wilayah di sepanjang pesisir Pantai dengan kondisi alam yang masih alami menyebabkan hasil tangkapan ikan nelayan sebagai perekonomian utama masyarakat Enggano, sangat melimpah. Tujuan dari pengabdian ini adalah meningkatkan kreativitas dan kualitas produksi variasi olahan ikan hasil tangkapan nelayan mempercepat proses pengolahan menggunakan presto, dengan packaging yang menarik melalui pemasaran online dan offline sehingga meningkatkan perekonomian masyarakat sekitar. Metode yang digunakan yaitu dengan penerapan difusi iptek dengan menghasilkan suatu produk bagi konsumen meliputi pemilihan bahan dan produksi Jompol Presto, Packaging, dan Pemasaran Produk. Dari hasil pengabdian yang telah dilaksanakan diperoleh bahwa warga telah mengetahui dan memahami penggunaan panci presto sebagai alat pemasak ikan jompol dan penggunaan plastik vacuum press sebagai packaging ikan jompol presto. Enggano Island as 3T region in Indonesia which is included in the North Bengkulu administrative area holds a variety of enormous natural resource potential. The position of the area along the coast with natural conditions that are still natural causes the catch of fishermen as the main economy of the Enggano community, very abundant. The purpose of this research is to increase the creativity and quality of production of processed fish variations of fishermen's catches by accelerating the processing process using pressure cook, with attractive packaging through online and offline marketing to improve the economy of the surrounding community. The method used is the application of science and technology diffusion by producing a product for consumers including material selection and production of Jompol Presto, Packaging, and Product Marketing. The results of the service that has been carried out, it is obtained that residents already know and understand the use of pressure cookers as a means of cooking jompol fish and the use of vacuum press plastic as packaging for presto jompol fish.
Co-Authors : Samwilson Slamet, : Samwilson ACENG RUYANI Adriansyah, Aulia Aini Nurul Anggraini, Putri Lisya Anisah, Saskia Anugrah, Ratu Intan Azhar, Afra Wafiqah Berutu, Ali Sadikin Berutu, Mei Awbina Cahlia, Uci Darmi, Darmi Dominica, Dwi Elfi Yuliza Ernis, Gustria Fadhila, Muhammad Adeng Fahmi, Riziq Ilham Nur Fatimatuzzahra Fatimatuzzahra Fitri, Sabrina Helmiyetti Hidayah, Thoriqul Ilfanisari, Vinolla Iman Rusmana Indah Medani, Della Jarulis Jarulis Jody, Regina Lingga, Rahmad Mashudi Mashudi Masrijal, Camelia Dwi Putri Morina Adfa Nabella, Inastia Nabila, Bella Nasution, Riski Padilah Nesbah, Nesbah NISA RACHMANIA MUBARIK Notriawan, Doni Oky Hermansyah Prastya, M. Eka Puspytha, Yolanda Reagen, Muhamad Alvin Reza Pertiwi Rina Suthia Hayu Rindiyani, Rindiyani Rizwar, Rizwar Rofiani, Desi Rothman, Dita Delia Safniyeti SALPRIMA YUDHA S Salsabilla, Sisilia Saputra, Apin Sari, Chaincin Buana Sari, Dhea Amelia Sasmita, Anggun SEMPURNA GINTING Setiawan, Redo Shufyani, Fahma Silvia, Elsi Simanjuntak, Putri Hezekiel Claracia Sinisuka, Alfredi Anis Fadhila Ginting Sipriyadi Suci Rahmawati, Suci Susanti, Elia Taifur, Aghus Thamara, Monika Thenawidjaya, Maggy Tri Sunardi, Tri Trianda, Aldy Wahyuni, Reza Welly Darwis Wulandari, Gustina Dwi Yar Johan, Yar