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Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera Asril, Muhammad; Lisafitri, Yuni
Jurnal Teknologi Lingkungan Vol. 21 No. 1 (2020)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.532 KB) | DOI: 10.29122/jtl.v21i1.3743

Abstract

ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam 
Rapid Land Cover Change in The South Sumatera Peat Area Associated With 2015 Peat Fires Raden Putra; Tastaptyani K Nufutomo; Yuni Lisafitri; Novi K Sari; Alfian Zurfi; Deni O Lestari; Muhammad U Nuha
Journal of Geoscience, Engineering, Environment, and Technology Vol. 7 No. 1 (2022): JGEET Vol 07 No 01 : March (2022)
Publisher : UIR PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/jgeet.2022.7.1.6395

Abstract

The peat fire events in Indonesia, particularly the South Sumatra area, changed the appearance of surface vegetation. The fires usually occur during the dry season from July to October. This study aims to evaluate land cover changes due to 2015’s peat fire in the South Sumatra peatlands. Remote sensing techniques using a Normalized Difference Vegetation Index (NDVI) method were used to identify the change of vegetation density in the study area. The results showed that 69% of the total South Sumatra peatland was burned due to the 2015 peat fire event. The level of vegetation density was considerably decreased by fire events. The degradation in the burned area was dominated by land cover class of ferns/shrub. The Peat fires during the observation period have a negative impact on the peat ecosystem, so improvements are needed in peatland management practices. Improvements need to be made in fire prevention and management practices, as well as restoration of burnt land.
DINAMIKA KELIMPAHAN ORIBATIDA PADA AREA PERKEBUNAN KELAPA SAWIT DI KECAMATAN BAJUBANG BATANGHARI JAMBI Yuni Lisafitri; Rahayu Widyastuti; Dwi Andreas Santosa
Jurnal Ilmu Tanah dan Lingkungan Vol 17 No 1 (2015): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.432 KB) | DOI: 10.29244/jitl.17.1.33-38

Abstract

Oribatids are one of the important fauna groups in the soil. They have an important role in the decomposition of organic matter in the soil. The objection of this research was to study population dynamics of Oribatids through determining the abundance and diversity of Oribatids and to know the effect of environmental factors on Oribatids in oil palm plantation at Bajubang, Batanghari, Jambi. Soil and litter samples were taken from four different locations (25 m x 25 m) and from each location three samples were taken from litter lanes and open lanes, respectively (the size was 16 x 16 cm with a depth of soil is 5 cm). The samples were extracted using Kempson extractor. Identification of Oribatids was done to the family level and the diversity of Oribatids was measured using Shannon’s diversity index. The population dynamic of Oribatids showed a fluctuation in the sampling period. When rainfall increased >200 mm did not follow by increasing abundance and diversity (number of the family) of Oribatid mites. When rainfall between 100 – 200 mm was the optimal condition for Oribatids so abundance and diversity (the number of the family) of Oribatids increased. But, when rainfall <100 mm especially in February 2014 (the lowest rainfall 1 mm), abundance and diversity (number of the family) of Oribatids was lowest (330 individual m-2; 7 families) compared to another month. It was assumed that Oribatids may migrate into the deep soil to avoid a harsh environmental condition. Abundance and diversity (number of the family) of oribatids were significantly higher in litter lanes (4,560 individual m-2; 25 families) than in open lanes (1,570 individual m-2; 14 families). The availability of litter in the litter lanes was higher than in open lanes. In this research, There are 3 families that most abundance and always found on the sampling period, they are Scheloribatidae, Mycobatidae and Galumniidae. Keywords: Abundance, diversity, oil palm plantation, Oribatids, soil fauna
Keterkaitan Sistem Penyediaan Air Bersih dan Angka Penyakit Diare di Daerah Pesisir Kelurahan Kangkung Esya Desfia Putri; Alfian Zurfi; Endang Setiawati; Yuni Lisafitri
Jurnal Ilmu Lingkungan Vol 20, No 1 (2022): January 2022
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.20.1.158-167

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Diare merupakan penyakit berbasis lingkungan yang sering dikaitkan dengan kejadian kematian. Kurang baiknya sistem penyediaan air bersih dapat meningkatkan kejadian diare. Tujuan penelitian ini untuk mengidentifikasi kejadian diare, mengkaji kualitas mikrobiologi sampel air, status sistem yang menyediakan air bersih, dan menganalisis korelasi antara angka penyakit diare dan sistem penyediaan air di daerah pesisir Kangkung, Bandar Lampung. Populasi pada kajian ini adalah seluruh masyarakat di wilayah pesisir Kelurahan Kangkung. Penentuan sampel dengan teknik random sampling. Data penelitian didapatkan melalui kuesioner dan analisis dilakukan dengan uji chi-square, sedangkan total coliform dilakukan dengan metode Most Probable Number. Hasil penelitian menunjukkan proporsi terjadinya diare sebanyak 22,2%, kandungan Coliform pada air PAM 0 MPN/ml, air suteng sebanyak 1100 MPN/ml, dan air sumur sebanyak 1100 MPN/ml. Daerah pesisir kelurahan Kangkung, sistem penyediaan air bersihnya cukup baik karena lebih banyak responden yang masuk dalam katagori memenuhi syarat dibandingkan yang tidak. Namun, hasil analisis menunjukkan tidak ada keterkaitan yang signifikan antara sistem yang menyediakan air bersih dengan kejadian diare di wilayah pesisir Kelurahan Kangkung.ABSTRACTDiarrhea is one of the disease based in environmentally that is often associated with death. Poor system of clean water supply can increase the incidence of diarrhea. The purpose of this research to identify frequency of diarrhea, to examine the microbiological quality of water samples, the status of the system that ptovides clean water, and to analize the correlation between diarrhea disease rate and the water supply system in the coastal areas of Kangkung, Bandar Lampung City. The population in this research were an entire comunity in the coastal area of Kangkung Village. Determination of the sample selection by the random sampling technique. The research data was obtained through a questionnaire and then analyzed was carried out using the chi-square test. Meanwhile, the total coliform test was carried out using the Most Probable Number method. The results showed the proportion of diarrhea was  22.2%, total Coliform in water from drink water company was 0 MPN/ml, Suteng water was 1100 MPN/ml, and well water was 1100 MPN/ml. In the coastal area of the Kangkung, the clean water supply system is quite good because more respondents are included in the eligible category than those who do not. However, the results of the analysis show that there is no significant correlation between the system that provides clean water and the incidence of diarrhea in the coastal area of Kangkung Village.
Preliminary Studies of Cassava Leaves’ Ability to Remove Dyes from Water Chairunnisa Chairunnisa; Yuni Lisafitri
Journal of Mathematical and Fundamental Sciences Vol. 52 No. 1 (2020)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2020.52.1.5

Abstract

Low-cost adsorbents from cassava leaves (CLP) were prepared by simply washing the leaf powder with deionized water. The adsorption of methylene blue (MB) from the aqueous solution was measured in order to examine the adsorbent performance. The effect of the pH of the solution was studied by varying pH from 2 to 10. The kinetics of MB adsorption onto CLP followed the pseudo-second-order kinetic model with a k-value of 0.087907 g mg-1 min-1 and 0.094081 g mg-1 min-1 for CLP before and after washing, respectively. The adsorption isotherm for both adsorbents followed the Langmuir isotherm model with qm values of 178.5 mg g-1 and 243.9 mg g-1. The existence of disturber ions was investigated by adding NaCl into the MB solutions at a variety of concentrations.
Identification of Land Cover Changes in The MK-PHR Conservation Area Associated With Fire Events Raden Putra; Tastaptyani Kurnia Nufutomo; Yuni Lisafitri; Novi Kartika Sari; Alfian Zurfi; Yanies Meiyanti
Indonesian Journal of Environmental Management and Sustainability Vol. 5 No. 1 (2021): March
Publisher : Research Centre of Inorganic Materials and Complexs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/ijems.2021.5.1.1-4

Abstract

Peat fire is one of the most environmental issues in Indonesia, particularly in the region of South Sumatra. The fires usually occur during the dry season from Juni to November. This study is designed to evaluate the impact of peat fires on changes in land cover in the Merang Kepahyang Peat Hydrological Region (MK-PHR) for the period of 2002 to 2015. The changes identified by the Normalized Difference Vegetation Index (NDVI) method using Landsat satellite imagery. The results of the study show that extreme peat fire associated with climate anomalies events occurred in 2006, 2012, and 2015. The largest peat fires during the observation period occurred in 2015 coincided with extreme climate anomalies generating negative changes in land cover in the most of MK-PHR areas. The changes occurred in almost half (49%) of the MK-PHR area, where it moved from the dense to moderate vegetation class. The spatial and temporal land cover changes will be discussed in more detail.
Antagonism Activity of Phosphate Solubilizing Bacteria Against Ganoderma philippii and Fusarium oxysporum of Acacia Plants Muhammad Asril; Yuni Lisafitri; Bayo Alhusaeri Siregar
Journal of Multidisciplinary Applied Natural Science Vol. 2 No. 2 (2022): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.118

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Ganoderma philippii and Fusarium oxysporum 0148c are the primary pathogenic fungi that causes root rot and damping-off in young acacia plants. The best treatment to date is the use of biological control agents. Phosphate solubilizing bacteria (PSB) isolated from acid soil is a bacterial isolate classified as plant growth-promoting bacteria (PGPB). PGPB has an indirect function as a biocontrol agent for fungal pathogens. This study aimed to determine the potential of PSB isolate EF.NAP 8 in inhibiting G. philippii and F. oxysporum 0148c from acacia plants. The method used is a dual culture antagonism test and observation of abnormal hyphae after the antagonism process. The results showed that the isolate EF.NAP 8 inhibited G. philippii by 34.44% and F. oxysporum 0148c by 33.33%. The abnormality of hyphae after antagonistic activity results in hyphal malformations such as hyphae lysis and hyphae coiling. The antagonistic activity of PSB EF.NAP 8 isolate is one of part of the ability of a bacterium classified as PGPB in the form of biocontrol activity against pathogenic fungi. This provides information regarding the opportunity to utilize EF.NAP 8 as a candidate agent for controlling fungal pathogens on acacia plants.
PEMETAAN CADANGAN KARBON MENGGUNAKAN CITRA RESOLUSI TINGGI UNTUK PENGELOLAAN TAHURA WAN ABDUL RACHMAN LAMPUNG (Carbon Stock Mapping Using High Resolution Image For Management Of Tahura Wan Abdul Rachman Lampung) Nirmawana Simarmata; Yuni Lisafitri; Dudung Muhally Hakim
Jurnal Sains Informasi Geografi Vol 2, No 1 (2019): Edisi Mei
Publisher : Universitas Muhammadiyah Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.744 KB) | DOI: 10.31314/jsig.v2i1.227

Abstract

Konversi lahan merupakan ancaman yang serius untuk kawasan TAHURA Wan Abdul Rachman dan berdasarkan peta citra landsat tahun 2010, kerusakan TAHURA mencapai 72%. Berdasarkan hasil perhitungan biofisik yang memungkinkanuntuk melakukan penilaian jasa pelayanan ekosistem terhadap hutan di kawasan TAHURA yang akan digunakan dalam pembangunan kawasan tersebut. Jasa pelayanan ekosistem hutan dapat dihitung melalui besaran biomassa dan serapan karbon yang hilang pada hutan tersebut. Estimasi kandungan biomassa dan karbon dapat dilakukan dengan memanfaatkan teknologi penginderaan jauh, melalui analisis regresi dari nilai NDVI Citra SPOT 6/7 yang dapat dikorelasikan dengan biomassa aktual. Distribusi biomassa di atas permukaan terdiri atas dua model yaitu peta model 1 dan model 2 yang diklasifikasikan menjadi 5 kelas kerapatan biomassa yakni kelas biomassa 1 model 1 berkisar antara -2,03 – 763,72 ton/ha, kelas 2 antara 763.72 – 1.658,09 ton/ha, kelas 3 antara 1.658,09 – 2.431,60 ton/ha, kelas 4 antara 2.431,60 – 2.963,38 ton/ha dan kelas 5 antara 2.963,38 – 3857,75. Kerapatan biomassa model 2, kelas 1 antara -1,242 - -7,71 ton/ha, kelas 2 antara -7,71 – 352,02 ton/ha, kelas 3 antara 353,02 – 663,14 ton/ha, kelas 4 antara 663,14 – 877,04 ton/ha, dan kelas 5 antara 877,04 – 1.236,77 ton/ha. Berdasarkan hasil estimasi Stok karbon model 1 memiliki nilai maksimum yaitu 616,36. Sementara stok karbon model 2 terdapat sekitar 964,435 ton/ha.
Education and Innovative Septic Tank: The Answer of Coastal Area Sanitation Problem Chairunnisa Chairunnisa; Yuni Lisafitri
Journal of Multidisciplinary Academic Vol 2, No 1 (2018): Science, Engineering and Social Science Series
Publisher : Penerbit Kemala Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Development of innovative septic tank for Sebesi island has been conducted followed by education the danger of defecation improperly. The reactor was made by using the concept of anaerobic baffled reactor with polyethylene based materials. In the other hand, the education project was successfully conducted that can be seen from the public enthusiasm built the sanitation system followed by the change of community behavior
Evaluation of hospital’s obedience in medical solid waste management Yuni Lisafitri; Merza Rahmawati; Rizka Firdausi Pertiwi; Bambang Prasetio
Journal of Multidisciplinary Academic Vol 1, No 1 (2017): Science, Engineering and Social Science Series
Publisher : Penerbit Kemala Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Waste is an environmental issue that quite unsettling right now. Hospitals are health facilities for community but also become one of waste contributor. Waste from hospital activities was called medical waste. Medical waste can be a source of disease for humans and other living organism and can disrupt environmental stability if not managed properly. The management of medical waste must be appropriate with regulations, but there are still many health facilities that have not managed according to the regulations. This study was conducted to evaluate of medical waste management in hospital to government regulation No. 101/2014 and Regulation of environment and forestry minister No. 56/2015. The research was conducted through several step from literature study to direct survey in the field through interviews and observations of the medical waste management. That information is evaluated in accordance with the waste management that has been regulated in government regulation No. 101/2014 and regulation of environment and forestry minister No. 56/2015. There are seven activities in the management of hazardous and toxic waste but this hospital only carry out two steps, such as collection and storage activities. Some of their doing in collection and storage activities was still not accordance with the regulations.