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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Ekologi Kesehatan AL KAUNIYAH Elkawnie Journal of Science and Applicative Technology Civil Engineering Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Agro Bali: Agricultural Journal Journal of Multidisciplinary Applied Natural Science

Muhammad Asril

Department Of Biology, Institut Teknologi Sumatera, Bandar Lampung-35365 (Indonesia)

Author-ID : 1038249

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Public Health

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Isolasi dan Seleksi Bakteri Proteolitik Asal Limbah Cair Tahu Sebagai Dasar Penentuan Agen Pembuatan Biofertilizer Muhammad Asril; Sovia Santi Leksikowati
Elkawnie: Journal of Islamic Science and Technology Vol 5, No 2 (2019)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v5i2.4356

Liquid tofu waste has the effect of damaging the environment if treated poorly. The waste contains a reasonably high protein so that it can be used as a substrate for isolation of proteolytic bacteria. Proteases produced by proteolytic bacteria can hydrolyze proteins in liquid tofu waste. The results of protein hydrolysis are amino acids in the form of nitrogen. The nitrogen can be used by plants for nutritional growth so that bioconversion products from proteolytic bacteria can be used as biofertilizers. The purpose of this study was to isolate and select potential proteolytic isolates from tofu liquid waste, which could be used as biofertilizer agents. The method used was bacterial isolation, proteolytic activity test, potato decay test, hemolysis test, and antagonism test among isolates. The results showed that there were two potential isolates from 28 isolates that had been successfully isolated, namely BLT-12 and BLT 21 with each Proteolytic Index of 3.20 and 3.16, respectively. The two isolates were gram-positive in the form of basil, did not have hemolysis activity, could not decompose potatoes so that they did not have a pathogenic effect on the test plants and did not have antagonistic properties between the two others. Therefore, BLT 12 and BLT 21 isolates can be used as candidates for potential isolates of biofertilizer agents.

KUALITAS BAKTERIOLOGIS MINUMAN THAI TEA PINGGIR JALAN: STUDI KASUS EMPAT KECAMATAN SEKITAR KAWASAN INSTITUT TEKNOLOGI SUMATERA DI PROVINSI LAMPUNG Muhammad Asril; Ika Agus Rini; Revi Agustin; Tarra Ivanka; Azisa Nabila Putri
JURNAL EKOLOGI KESEHATAN Vol 20 No 1 (2021): JURNAL EKOLOGI KESEHATAN VOLUME 20 NOMOR 1 TAHUN 2021
Publisher : Puslitbang Upaya Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jek.v20i1.4636

ABSTRACT Beverages that are widely sold on the roadside are very easily contaminated by various types of bacteria that are harmful to health. This study aims to detect the presence of bacteria in Thai tea drinks sold on the roadside along the Sumatran Institute of Technology (ITERA) area. The research design was cross-sectional using a descriptive approach which was carried out in October 2019. Detection of bacteria using specific medium Desoxycholate Lactose Sucrose (DCLS) and Cystine Lactose Electrolyte Deficient (CLED) from 25 samples located in 4 villages around the ITERA area. A total of 25 samples were tested, all Thai tea samples were contaminated with bacteria and did not qualify as a drink fit for consumption. Thai tea from Tanjung Happy (TS) District has the highest Enterobacteriaceae contamination, namely 55-65x104 cfu/ml, namely Klebsiella sp. (30x105 cfu/ml), E. coli (15x105 cfu/ml), Salmonella sp. (15x105 cfu/ml) and Proteus sp. (15x105 cfu/ml). The presence of these bacteria is an early indication of the potential for disease and the seller's lack of personal hygiene which includes water sources and raw materials for making Thai tea. Therefore, knowledge to sellers regarding overall cleanliness needs to be improved. Keywords: Beverages, bacteria Contaminant, Lampung Province, Thai tea ABSTRAK Berbagai jenis minuman yang banyak dijual dipinggir jalan sangat mudah terkontaminasi oleh berbagai jenis bakteri yang membahayakan kesehatan. Penelitian ini bertujuan untuk mendeteksi keberadaan bakteri dalam minuman Thai tea yang dijual dipinggir jalan sepanjang kawasan Institut Teknologi Sumatera (ITERA). Disain penelitian adalah potong lintang menggunakan pendekatan deskriptif yang dilakukan pada bulan Oktober 2019. Deteksi bakteri menggunakan medium spesifik Desoxycholate Lactose Sucrose (DCLS) dan Cystine Lactose Electrolyte Deficient (CLED) dari 25 sampel yang berada di 4 kelurahan di sekitar wilayah ITERA. Sebanyak 25 sampel yang diuji, seluruh sampel Thai tea terkontaminasi oleh bakteri dan tidak memenuhi syarat sebagai minuman layak konsumsi. Thai tea yang berasal dari Kecamatan Tanjung Senang (TS) memiliki cemaran Enterobacteriaceae tertinggi yaitu 55-65x104 cfu/ml, yaitu Klebsiella sp. (30x105 cfu/ml), E. coli (15x105 cfu/ml), Salmonella sp. (15x105 cfu/ml) dan Proteus sp. (15x105 cfu/ml). Keberadaan bakteri ini merupakan indikasi awal adanya potensi munculnya penyakit dan rendahnya kebersihan pribadi penjual yang melingkupi, sumber air dan bahan baku pembuatan Thai tea. Pengetahuan penjual terkait kebersihan secara keseluruhan perlu ditingkatkan. Kata kunci: Minuman, bakteri kontaminan, Provinsi Lampung, Thai tea

Keanekaragaman Jenis Burung di Kawasan Pengembangan Institut Teknologi Sumatera (ITERA) Indah Oktaviani; Yanti Ariyanti; Sovia Santi Leksikowati; Muhammad Asril
Al-Kauniyah: Jurnal Biologi Vol 14, No 1 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i1.12323

AbstrakKawasan kampus Institut Teknologi Sumatera (ITERA) sedang mengalami perkembangan fisik yang pesat, seperti pembangunan gedung perkuliahan, laboratorium, asrama, juga embung untuk sumber air. Informasi mengenai keanekaragaman hayati di ITERA sendiri belum banyak diteliti, salah satunya adalah keanekaragaman burung. Burung merupakan hewan besar yang cukup sensitif dengan perubahan lingkungan. Penelitian ini dilakukan untuk mendapatkan data mengenai keanekaragaman burung di ITERA untuk memberikan informasi dasar. Metode yang digunakan adalah teknik point count, diterapkan pada lima stasiun utama yang dipilih berdasarkan pusat aktivitas pembangunan. Analisis yang digunakan adalah indeks keanekaragaman, kemerataan, juga kelimpahan. Selain itu, status konservasi dan tipe pakan jenis burung dianalisis menggunakan data sekunder. Hasil penelitian menunjukkan terdapat 19 jenis burung dari 16 famili. Keanekaragaman jenis burung berdasarkan indeks Shannon-Wiener tergolong sedang dan kemerataan jenis merata. Dari kategori kelimpahan yang digunakan terdapat dua jenis burung yang umum ditemukan. Bersumber pada PP No. 7 tahun 1999, terdapat empat jenis burung di ITERA yang termasuk ke dalam kategori dilindungi dan satu jenis termasuk kategori Appendix II di CITES. Feeding guild burung di ITERA didominasi tipe omnivora dan insektivora. AbstractInstitut Teknologi Sumatera (ITERA) campus area is undergoing rapid physical development, such as the construction of lecture buildings, laboratories, dormitories, as well as reservoirs for water sources. Information on biodiversity in ITERA has not much studied, one of which is bird diversity. Birds are large animals that are quite sensitive to environmental changes. This study aims to obtain data on bird diversity at ITERA to provide the baseline information. The method used is a point count technique, applied to five main stations selected based on the center of development activities. The analysis used is an index of diversity, evenness, and abundance. Besides, the conservation status and feed type of bird species were analyzed using secondary data. The results showed that there were 19 species of birds from 16 families. The Shannon-Wiener diversity index classified the bird community as a moderate, and the community evenness index was stable. From the abundance category, two types of birds commonly found. Based on PP No. 7 of 1999, there are four species of birds in ITERA, which included in the protected category and one species, including the Appendix II category in CITES. Omnivorous and insectivorous types dominate bird guild feeding in ITERA.

Antagonism Activity of Phosphate Solubilizing Bacteria Against Ganoderma philippii and Fusarium oxysporum of Acacia Plants Muhammad Asril; Yuni Lisafitri; Bayo Alhusaeri Siregar
Journal of Multidisciplinary Applied Natural Science Vol. 2 No. 2 (2022): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.118

Ganoderma philippii and Fusarium oxysporum 0148c are the primary pathogenic fungi that causes root rot and damping-off in young acacia plants. The best treatment to date is the use of biological control agents. Phosphate solubilizing bacteria (PSB) isolated from acid soil is a bacterial isolate classified as plant growth-promoting bacteria (PGPB). PGPB has an indirect function as a biocontrol agent for fungal pathogens. This study aimed to determine the potential of PSB isolate EF.NAP 8 in inhibiting G. philippii and F. oxysporum 0148c from acacia plants. The method used is a dual culture antagonism test and observation of abnormal hyphae after the antagonism process. The results showed that the isolate EF.NAP 8 inhibited G. philippii by 34.44% and F. oxysporum 0148c by 33.33%. The abnormality of hyphae after antagonistic activity results in hyphal malformations such as hyphae lysis and hyphae coiling. The antagonistic activity of PSB EF.NAP 8 isolate is one of part of the ability of a bacterium classified as PGPB in the form of biocontrol activity against pathogenic fungi. This provides information regarding the opportunity to utilize EF.NAP 8 as a candidate agent for controlling fungal pathogens on acacia plants.

Isolasi Cendawan yang Berperan dalam Proses Pembuatan Pliek U (Makanan Fermentasi Khas Aceh) Muhammad Asril; Analekta Tiara Perdana; Mahyarudin Mahyarudin; Anja Asmarany; Qurrota A’yun
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 1 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Pliek u merupakan produk residu hasil fermentasi kelapa oleh mikroorganisme tertentu secara spontan. Produk hasil fermentasi ini telah dimanfaatkan secara turun temurun oleh masyarakat Aceh sebagai bahan bumbu masak seperti pembuatan sayur, olahan ikan dan sambal buah-buahan, sedangkan minyak yang dihasilkan selama proses fermentasi dijadikan sebagai obat-obatan. Penelitian ini bertujuan untuk mengetahui jenis cendawan yang berperan sebagai fermentor alami selama proses pembuatan pliek u. Cendawan diisolasi dari substrat kelapa yang telah terfermentasi selama 5 hari dan ditumbuhkan di media Potato Dextrose Agar. Pengamatan makroskopis dilakukan dengan mengamati warna spora dan warna misellium, sedangkan pengamatan mikroskopis dilakukan dengan teknik slide culture yaitu mengamati bentuk dan ukuran spora. Hasil penelitian menunjukkan bahwa terdapat 9 isolat cendawan yang terdiri dari 6 isolat dari genus Aspergillus yaitu A. niger 1, A. niger 4.1, A. niger 5, A. flavus 3, A. flavus 4.2, A. flavus 6. Sedangkan 3 isolat lainnya merupakan Penicillium citrinum, Thielaviopsis paradoxa dan Geotrichum candidum. Keberadaan cendawan ini dipengaruhi oleh substrat yang digunakan pada fermentasi pliek u. Selain itu, juga diperoleh minyeuk simplah yang berwarna kuning dan merupakan produk samping selama proses fermentasi pliek u. Hasil ini menegaskan bahwa cendawan yang berperan dalam proses pembuatan pliek u sangat beragam tergantung proses pembuatannya.

ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL IAA (INDOLE ACETIC ACID) DARI RHIZOSFER TANAMAN AKASIA (Acacia mangium) Ika Agus Rini; Indah Oktaviani; Muhammad Asril; Revi Agustin; Fina Khaerunissa Frima
Agro Bali : Agricultural Journal Vol 3, No 2 (2020)
Publisher : Universitas Panji Sakti

IAA adalah produk paling umum dari metabolisme L-triptofan yang dapat diproduksi oleh beberapa mikroorganisme. Beberapa mikroorganisme yang memiliki potensi menghasilkan IAA adalah bakteri rhizosfer pada tanaman Leguminosae, salah satunya adalah akasia. Acacia mangium, juga dikenal sebagai akasia, adalah pohon yang tumbuh cepat. Namun, akasia adalah tanaman invasif. Tanaman akasia memiliki bintil yang merupakan hasil simbiosis akar tanaman dan bakteri. Simbiosis ini dapat mempengaruhi kesuburan tanah. Banyak potensi yang dapat digali dari bakteri tanah, khususnya di rhizosfer. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri pada rizosfer tanaman akasia yang mampu menghasilkan IAA sebagai salah satu potensi untuk kandidat PGPR (Plant Growth Promoting Rhizobacteria). Metode yang digunakan meliputi pengambilan sampel, isolasi bakteri penghasil IAA, pemurnian bakteri, identifikasi bakteri dan uji biokimia, pembuatan kurva tumbuha bakteri, dan uji isolat bakteri pelarut fosfat. Hasil isolasi bakteri rhizosfer diperoleh sebanyak 10 isolat bakteri yang memiliki karakteristik berbeda secara morfologi. Berdasarkan hasil identifikasi bakteri berdasarkan pewarnaan Gram, bakteri tersebut masuk ke dalam genus Bacillus dan terdapat 5 isolat bakteri yang memiliki kemampuan menghasilkan IAA dan melarutkan fosfat sehingga bakteri tersebut memiliki potensi sebagai pupuk hayati.

Airborne Microbial Quality Assessment in the Educational Buildings during the COVID-19 Pandemic Muhammad Asril; Salsabila Sugiarto; Alfian Zurfi
Civil Engineering Journal Vol 9, No 1 (2023): January
Publisher : Salehan Institute of Higher Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/CEJ-2023-09-01-09

Rooms with pollutants have a poor impact of 2-5 times greater than outdoors. The lecture hall had the potential to experience a decrease in air quality. This study was conducted to assess microbiological air quality in the general lecture building I Institut Teknologi Sumatra, Lampung, Indonesia, during the COVID-19 pandemic and its relationship with environmental conditions. This study was conducted using a settling sampling technique to count the number of bacteria and fungi in the air. Samples were collected twice daily for five working days. The results showed that the highest concentrations of bacteria and fungi were found at the wifi corner location, at 36.7–1237.2 CFU/m3 and 225.4–1431.2 CFU/m3, respectively. The highest average concentrations of bacteria and fungi at the wifi corner location were found in the afternoon at 479.1 ± 438.1 CFU/m3 and 800 ± 548.4 CFU/m3, respectively. The three locations did not meet the standards of room suitability for humans with immunodeficiencies based on the ACGIH. The location of the wifi corner did not meet the standards of the Minister of Manpower of the Republic of Indonesia No. 5 of 2018 and the WHO regarding microbial standards in the room. The highest value of the Global Microbial Contamination Index (GIMC/m3) was found in the wifi corner (G4: ≥2000 – ≤4000), which is closely related to population density and ventilation. Environmental factors influence the density of bacteria and fungi at the sampling sites. The relative humidity strongly influenced the concentration of fungi. In addition to relative humidity, bacterial density is also affected by light intensity and the number of people. This indicates that despite restrictions on activities during the COVID-19 pandemic, the room at GKU 1 did not meet the eligibility requirements for students with immune disorders. Doi: 10.28991/CEJ-2023-09-01-09 Full Text: PDF

A systematic survey of plant biodiversity study within the land of Institut Teknologi Sumatera (ITERA) I. Oktaviani; M. Asril; Y. Aryanti; S. S. Leksikowati
Journal of Science and Applicative Technology Vol 2 No 1 (2019): ICoSITeR Special Edition
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

The conversion of agricultural land and plantation into an area with high human activity can affect the biodiversity contained in it. The biodiversity of a region can be surveyed and collect in a systematic database to know the wealth of flora and fauna resources within a certain period of time. The aim of this research is to identify and inventory the vegetation database of plant, which can then be used as one of the important information related to the environmental change that happened from time to time following the impact of the growing development expansion. This study was conducted within the campus of the Institut Teknologi Sumatra (ITERA) which is undergoing physical construction in the form of buildings, roads, and some retention basin. Data retrieval was done by direct observation using the cruising method. The observations of each species are documented, identified, recorded and arranged in the collection catalog.

UJI POTENSI Bacillus sp. DAN Escherichia coli DALAM MENGHASILKAN INDOLE ACETIC ACID (IAA) TANPA MENGGUNAKAN TRIPTOFAN PADA MEDIA PERTUMBUHAN Muhammad Asril
Journal of Science and Applicative Technology Vol 1 No 2 (2017): Journal of Science and Applicative Technology December Chapter
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Indole Acetic Acid (IAA) is the important phytohormone for boosting plant-growth promotion. It is related with amino acid tryptophan as a precursor for building IAA. However some bacteria such as Bacillus sp. and Escherichia coli enable to produce IAA without L-tryptophan on culture medium via the tryptophan independent pathways. This study aims to determine the potential of indigenous Bacillus sp. and E. coli isolate in producing IAA via Trp-independent pathways (without L- tryptophan). The stage of this study consists of growing of isolate on liquid media without tryptophan and measuring the levels of IAA using Salkowsky method. Bacillus sp and Escherichia coli could produce IAA with the concentration of 0,0236 mmol/L and 0,024 mmol/L, respectively. The concentration of IAA produced by these bacteria is quite low because of their ability to synthesis IAA from the independent tryptophan pathway. The independent tryptophan pathway means these bacteria could synthesize IAA without using tryptophan as a precursor on their growth medium.

Bioprospeksi Bakteri Asal Akar Nanas (Ananas comosus L. Merr) Lahan Gambut Kayu Agung, Sumatra Selatan, sebagai Agen Biostimulan dan Bioprotektan Erma Suryanti; Dewi Chusniasih; Muhammad Asril; Ika Agus Rini; Wulandari Putri Antika; Nadia Rahmah
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 3 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.3.352

Pineapple (Ananas comosus L. Merr) is a plant that can live well on nutrient-poor lands such as peatlands. However, plant resistance to nutrient-poor conditions is caused by symbiosis with endophytic bacteria capable of plant growth-promoting bacteria (PGPB) through biostimulant and bioprotective mechanisms. This study aims to characterize the potential of endophytic bacteria from pineapple roots from peatlands as biostimulants and bioprotective agents. Thirteen endophytic bacteria from pineapple root were characterized by their ability as biostimulants through phosphate dissolution tests using Pikovskaya media qualitatively and quantitatively, and the production of indole-3-acetic acid (IAA) upon additional 0.1% tryptophan. Furthermore, bacteria as bioprotectors were characterized by qualitative production of chitinase enzyme and antifungal tests against Fusarium proliferatum using dual culture techniques. The results showed the presence of 2 positive bacteria in all tests, namely the ANAP3 and ANAP5 isolates. ANPA3 bacteria show the highest activity in IAA production (26.3 ppm), and the highest antifungal activity in inhibiting F. proliferatum, with an inhibitory index reaching 52.6%. Meanwhile, ANAP5 is an endophytic bacterium with the highest phosphate dissolving activity, with a dissolved phosphate value of 253.5 ppm. This finding shows that some endophytic bacteria from pineapple roots on peatlands can be biostimulants and bioprotectors that can be developed as PGPB. Keywords: Ananas comosus L. Merr, endophytic bacteria, bioprotectant, biostimulant, Fusarium proliferatum

Co-Authors Alfian Zurfi Analekta Tiara Perdana Anja Asmarany Apriza Yuswan Azisa Nabila Putri Bayo Alhusaeri Siregar Dewi Chusniasih Dwi Retnowati Erma Suryanti Fina Khaerunissa Frima I. Oktaviani Ika Agus Rini Ika Agus RINI Ika Agus Rini Indah D. Dewijanti Indri Yati Irmanida Batubara Leksikowati, Sovia Santi Mahyarudin, Mahyarudin Muhammad Eka Prastya Nadia Rahmah Novita Ariani Oktaviani, Indah Qurrota Ayun Revi Agustin Revi Agustin Riana, Elisa Nurma Salsabila Sugiarto Tarra Ivanka Tjandrawati Mozef Vera Permatasari Wulandari Putri Antika Y. Aryanti Yanti Ariyanti Yuni Lisafitri

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