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All Journal Buletin Veteriner Udayana
I Gusti Ngurah Bagus Trilaksana
Laboratorium Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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LEVEL OF KNOWLEDGE, ATTITUDE AND BEHAVIOR OF THE COMMUNITY IN BANJAR PANDE AND BANJAR PASEKAN TOWARDS THE ROLE OF WOLBACHIA MOSQUITOES AS A STRATEGY TO CONTROL DENGUE FEVER I Made Gede Asta Ginawan; I Made Sukada; Romy Muhammad Dary Mufa; Kadek Karang Agustina; I Wayan Masa Tenaya; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p26

Abstract

Wolbachia is a naturally occurring bacterium found in various insects, such as fruit flies, butterflies, and moths. It has been utilized as an innovative strategy to control the spread of dengue fever by releasing Wolbachia-infected Aedes aegypti mosquitoes. This study aimed to assess the public’s understanding of the benefits of Wolbachia-carrying mosquitoes as a dengue control measure. Data were collected through questionnaire-based interviews with residents of Banjar Pande and Banjar Pasekan in Kaba-Kaba Village, Kediri District, Tabanan Regency. Pre-test results revealed that the level of knowledge among Banjar Pande residents was 50%, attitude 45%, and behavior 66%. Meanwhile, in Banjar Pasekan, pre-test scores for knowledge, attitude, and behavior were 47%, 47%, and 60%, respectively. These findings indicate that, prior to educational interventions, community awareness, attitudes, and behavior regarding Wolbachia mosquitoes as a dengue control strategy were at a moderate level. Post-test results demonstrated significant improvements, with the community exhibiting deeper knowledge, increased concern, and greater acceptance of Wolbachia technology. This study concludes that targeted education effectively enhances public awareness and support for Wolbachia as a sustainable dengue prevention approach.
QUALITY OF DIUTED BOAR SEMEN USING PALMYRA FRUIT WATER WITH THE ADDITION OF VARIOUS CONCENTRATIONS OF EGG YOLK AND MORINGA LEAF EXTRACT I Made Gede Raditya Darmayoga; Wayan Bebas; Luh Gde Sri Surya Heryani; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; I Wayan Sukernayasa
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p30

Abstract

Diluent is a substance used to maintain and protect spermatozoa during the storage process. Natural-based diluents are still rarely found, making it necessary to develop diluents derived from naturally available ingredients. This study aims to evaluate the effect of palmyra fruit water diluent with the addition of various concentrations of egg yolk and moringa leaf extract on the quality of boar semen. This study used one Landrace boar as the semen source, approximately 1.5 years old, in healthy condition. The study employed a Completely Randomized Design CRD with seven treatments: T0 as the control palmyra fruit water diluent only, T1 palmyra fruit water + 10% egg yolk + 5% moringa leaf extract, T2 palmyra fruit water + 10% egg yolk + 10% moringa leaf extract, T3 palmyra fruit water + 10% egg yolk + 15% moringa leaf extract, T4 palmyra fruit water + 20% egg yolk + 5% moringa leaf extract, T5 palmyra fruit water + 20% egg yolk + 10% moringa leaf extract, and T6 palmyra fruit water + 20% egg yolk + 15% moringa leaf extract. The semen was stored at a temperature of approximately 15-20°C for 48 hours. The observed semen quality parameters included motility, viability, and spermatozoa abnormalities, with each treatment repeated four times. The obtained data were analysed using ANOVA, followed by Duncan's test if significant differences were found. The results of Duncan's test indicated that the T6 treatment produced the best semen quality compared to other treatments, with motility at 59.50±1.291%, viability at 59.25±1.708%, and abnormalities at 7.25±0.500%. It can be concluded that the diluent formulation that resulted in the best spermatozoa quality after 48 hours of storage was treatment T6, composed of palmyra fruit water + 20% egg yolk + 15% moringa leaf extract. Further research is needed to determine the fertility potential of Landrace boar semen diluted with palmyra fruit water extender with the addition of various concentrations of egg yolk and moringa leaf extract to evaluate the effectiveness of the extender in an artificial insemination program.
QUALITY OF BOAR SEMEN EXTENDED IN PALM WATER-EGG YOLK DILUENT SUPPLEMENTED WITH A. DORSATA AND TRIGONA SP. HONEY Maria Patrisila Naibina; Wayan Bebas; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun; Desak Nyoman Dewi Indira Laksmi; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p27

Abstract

One of the factors that determine the success of artificial insemination in pig livestock is the quality of the semen used. The use of fresh semen without going through the dilution process will result in a decrease in the quality of spermatozoa. This study aims to determine the quality of Landrace pig semen in egg yolk palm water dilution with the addition of Apis dorsata and Trigona Sp. bee honey with a concentration of 3% each. This study used a complete random design pattern with five treatment groups, namely Beltsville Thawing Solution (BTS) diluent as a control (P0), palm fruit water diluent (P1), egg yolk palm water diluent (P2), egg yolk palm water diluent plus A. dorsata honey (P3) and egg yolk palm water diluent plus Trigona Sp. honey (P4). The diluted cement is stored at 150C for 48 hours. The observed semen quality parameters are motility, abnormality, viability, intact plasma membrane and intact acrosome membrane. The results showed that the water semen dilution of egg yolk palm fruit with the addition of A. dorsata honey with a concentration of 3% resulted in the highest motility, viability, intact plasma membrane and intact acrosome membrane and the lowest spermatozoa abnormalities when compared to Trigona Sp. honey and other treatments. It can be concluded that the egg yolk palm fruit water dilutor with the addition of A. dorsata honey with a concentration of 3% is able to maintain the quality of Landrace pig spermatozoa stored at 150C for 48 hours. Therefore, it is necessary to conduct further research on the fertility and litter size of Landrace pigs that are inseminating with egg yolk palm fruit water diluent with the addition of A. dorsata honey with a concentration of 3%.
Co-Authors Desak Nyoman Dewi Indira Laksmi Desak Nyoman Dewi Indira Laksmi I Made Gede Asta Ginawan I Made Gede Raditya Darmayoga I Made Sukada I Wayan Masa Tenaya I Wayan Sukernayasa Kadek Karang Agustina Luh Gde Sri Surya Heryani Maria Patrisila Naibina Ni Nyoman Werdi Susari Romy Muhammad Dary Mufa Tjok Gde Oka Pemayun Wayan Bebas Wayan Bebas