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All Journal Kwaghe International Journal of Sciences and Technology African Journal of Clinical Medicine and Pharmacy Research
Maianguwa Abdulrashid Dauda
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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Isolation and Identification of Phenol Degrading Bacteria in Refuse Dumping Site Muhammad Bashir Muhammad; Abubakar Aisami; Maianguwa Abdulrashid Dauda; Abdullahi Sa’adu Adeseye; Isaac John Umaru; Helen Oluwabunmi; Bilyaminu Habibu; Gloria Omonefe Oladele
Kwaghe International Journal of Sciences and Technology Vol 1 No 1 (2024): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v1i1.3686

Abstract

Phenol is a harmful compound found in soil, and its removal is crucial for human health. Phenol or hydroxybenzene, is both a synthetically and naturally produced aromatic compound. Microorganisms capable of degrading phenol are common and include both aerobes and anaerobes. In this study, researchers collected soil samples from INEX refuse dumping site along Bajoga road of kwami, Gombe state, and determined the abundance of heterotrophic and degradation bacteria using serial dilution and most probable number (MPN) methods. This experimental research study was carried out in order to isolate and identify phenol-degrading bacteria in polluted soil, the effect of concentration and pH was analysed during the procedure. Bacteria was isolated from contaminated soil and sub-cultured in a Mineral-salt media which was prepared with phenol added as the only carbon source needed for the bacterial growth, The bacterial isolate was identified as Escherichia coli a gram-negative bacteria. The favourable concentration and pH required for the growth of the bacteria was determined as 200mg/L and pH 7 respectively. These findings have significance in bioremediation for employing suitable bacteria in suitable condition for solving environmental pollution crises.
In-Vitro Estimation of Total Phenolic, Flavonoid, Antioxidant Contents and Determination of Flavonoid, Amino Acid, and Vitamin Profiles of Ethanol Leaf Extract of Annona squamosa L. Ojochenemi Ejeh Yakubu; Jankada Patience Audu; Arowora Kayode Adebisi; Danjuma Janya; Abu Michael Sunday; Shitta Nasisi Namuma; Fidelis Okii Ekpo; Maianguwa Abdulrashid Dauda; Josephine Maina Sule; Isaac John Umaru
African Journal of Clinical Medicine and Pharmacy Research Vol 1 No 1 (2024): African Journal of Clinical Medicine and Pharmacy Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajcmpr.v1i1.3865

Abstract

Since the beginning of human civilisation, plants have been one of the main sources of medicines. The demand for pharmaceuticals, health products, food supplements, cosmetics, and medications made from plants is expanding. Since ancient times, traditional medicine has made use of the medicinal plant Annona squamosa. The aim of this research is to evaluate the total phenolic, flavonoid, antioxidant contents and the amino acid, and vitamin profiles of ethanol leaf extract of Annona squamosa L. By using the DPPH free radical scavenging experiment, the antioxidant potential of the ethanol leaf extracts was calculated, and the absorbance was determined at 517 nm. The IC50 value of the leaf was estimated to be 4.24 μg/mL. The Folin Ciocalteu method was used to measure the total phenolic content (TPC) spectrophotometrically. Total flavonoid content (TFC) was measured by aluminium chloride colorimetric assay and Quercetin was used as standard, the absorbance was measured at 520nm. The results showed that A. squamosa leaf is a rich source of phenolics and flavonoid (total Phenolic content: 26.01±1.45mg GAE/g and total flavonoid content: 71.20±35.74 mg QE/g). The high contents of phenolic compounds and flavonoid contents indicated that these compounds contribute to the antioxidant activity. A. squamosa can be regarded as promising plant species for natural plant sources of antioxidants with high potential value for drug preparation. The total amount of amino acids was found to be 331.887 uL Several detection and peak resolution were obtained from the HPLC analysis. The result showed the presence of eight amino acids including Asparagine (2.419uL), Threonine (50.828uL), Phenylalanine (37.533uL), Aspartic Acid (144.558uL), Leucine (8.650uL), Iso-Leucine (9.728uL), Glycine (54.060uL) and four vitamins Vitamin K (2.289mg), Vitamin B1 (958.597mg), Vitamin A (13.568mg) and Vitamin E (62.103mg).
Co-Authors Abdullahi Sa’adu Adeseye Abu Michael Sunday Abubakar Aisami Arowora Kayode Adebisi Bilyaminu Habibu Danjuma Janya Fidelis Okii Ekpo Gloria Omonefe Oladele Helen Oluwabunmi Isaac John Umaru Jankada Patience Audu Josephine Maina Sule Muhammad Bashir Muhammad Ojochenemi Ejeh Yakubu Shitta Nasisi Namuma