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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Articles 38 Documents
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Search results for , issue "Vol. 6 No. 1 (2019): June 2019" : 38 Documents clear
Preface JBBI Vol 6, No 1, June 2019: Foreword and Acknowledgement Tajudin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.343 KB) | DOI: 10.29122/jbbi.v6i1.3694

Abstract

EFFECT OF TROGLITAZONE ON MORPHOLOGICAL CHANGES AND PPAR-γ GENE EXPRESSION IN 3T3-L1 ADIPOCYTE CELLS Sulfianti, Asri; Triwulansari, Mayriska; Nuralih, .; Churiyah, .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (820.23 KB) | DOI: 10.29122/jbbi.v6i1.3117

Abstract

Efek Troglitazone terhadap Perubahan Morfologi dan Ekspresi Gen PPAR- γ di Dalam Sel Adiposa 3T3-L1 ABSTRACT3T3-L1 cells are extensively used as a model to study adipogenesis. However, one major concern is the prolonged period of time it takes the cells to differentiate into adipocytes form. To induce this differentiation, the adipogenic induction media is required. In this study, troglitazone, a hypoglycemic agent was added to adipogenic induction media and observed in order to determine the morphological changes and peroxisome proliferator-activated receptor gamma (PPAR-γ) gene expression in 3T3-L1 differentiation. It is generally known that PPAR-ꝩ plays an important role as a transcription factor in adipocyte differentiation. Based on Oil Red O Staining, adipogenic induction with or without troglitazone changed the 3T3-L1 pre-adipocytes into mature round fat cells characterized by red droplet lipids. This cell also had a high absorbance level and degree of droplet accumulation of P≤ 0.05 in each group. In addition, cells treated by troglitazone had the highest PPAR-ꝩ mRNA level (1.9 fold) than those treated by adipogenic induction media without troglitazone or cells un-treated at all. Keywords: 3T3-L1, adipocyte, differentiation, PPAR-ꝩ, troglitazone ABSTRAKSel 3T3-L1 adalah jenis sel yang banyak digunakan dalam studi adipogenesis. Namun, salah satu kelemahan sel tersebut adalah lamanya waktu yang dibutuhkan bagi sel pre-adiposa untuk berdiferensiasi menjadi sel adiposa. Selain itu, dibutuhkan pula media induksi khusus untuk mengubah sel menjadi sel adiposa. Pada penelitian ini, kami mengobservasi fungsi troglitazone, sebagai antidiabetes terhadap perubahan morfologi dan ekspresi gen peroxisome proliferator-activated receptor gamma (PPAR-γ). Telah diketahui bahwa PPAR-ꝩ berperan penting sebagai factor transkripsi dalam diferensasi sel adiposa. Berdasarkan pewarnaan ORO, induksi sel pre-adiposa 3T3-L1 dengan media induksi dengan dan tanpa troglitazone merubah sel preadiposa menjadi sel berbentuk bulat yang dikarakterisasi dengan akumulasi droplet lemak. Nilai absorbansi sel adiposa juga menandakan adanya perbedaan yang signifikan antara kelompok sel yang diberi troglitazone dan tidak, dan sel tanpa diberi media induksi. Sementara, pada kelompok sel yang diberi troglitazone memiliki ekspresi mRNA PPAR-ꝩ (1,9 kali) tertinggi jika dibandingkan dengan sel yang diberi media induksi tanpa troglitazone, dan tanpa media induksi sama sekali.Kata Kunci: 3T3-L1, adiposa, diferensiasi, PPAR-ꝩ, troglitazone
PERBANDINGAN TIGA KIT EKSTRAKSI RNA UNTUK ANALISIS TRANSKRIPTOMIKA PADA KELAPA SAWIT (Elaeis guineensis Jacq.) Zulaeha, Siti; Purwoko, Devit; Cartealy, Imam; Tajuddin, Teuku; Karyanti, .; Khairiyah, Hayat
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.283 KB) | DOI: 10.29122/jbbi.v6i1.3372

Abstract

Comparison of Three RNA Extraction Kits for Transcriptome Analysis of Oil Palm (Elaeis guineensis Jacq.) ABSTRACTObtaining high-quality RNA is very important at an early stage of molecular biology research. To isolate RNA, high skill and caution are required in following laboratory procedures because RNA is easily degraded, especially samples from plant tissue culture. One of the parameters used to check the total RNA quality is RIN (RNA Integrity Number). The aim of this study was to obtain RNA extraction methods on oil palm leaves, callus and somatic embryos that were of good quality and high concentrations for transcriptomic analysis. RNA extraction was carried out using Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) and RibospinTM Plant (Geneall) kit methods. The results showed that oil palm leaf, callus and somatic embryo RNA were successfully extracted using the RibospinTM (Geneall) kit. Based on the total RNA number of more than 4 μg and the RIN value of more than 7, the extracted RNA could be used in RNA sequencing for transcriptomic analysis. Keywords: callus, oil palm, RNA analysis, RNA quality, somatic embryo ABSTRAKMenghasilkan RNA berkualitas tinggi sangatlah penting pada tahap awal penelitian biologi molekuler. Untuk mengisolasi RNA diperlukan keterampilan dan kehati-hatian tinggi dalam mengikuti prosedur di laboratorium karena RNA lebih mudah terdegradasi, khususnya sampel hasil kultur jaringan tanaman. Salah satu parameter yang digunakan pada pengecekan kualitas RNA total adalah RIN (RNA Integrity Number). Penelitian bertujuan mendapatkan metode ekstraksi RNA pada daun, kalus dan embrio somatik kelapa sawit yang berkualitas baik dan memiliki konsentrasi tinggi untuk analisa transkriptomika.  Ekstraksi RNA dilakukan menggunakan metode kit Plant RNA PureLink (Ambion), Genezol RNA Extraction (Geneaid) dan RibospinTM Plant (Geneall). Hasil menunjukkan bahwa RNA daun, kalus dan embrio somatik kelapa sawit telah berhasil diekstraksi dengan menggunakan kit RibospinTM (Geneall). RNA hasil ekstraksi tersebut dapat digunakan untuk sekuensing RNA dengan tujuan analisis transkriptomika, dilihat dari jumlah total RNA yang lebih dari 4 μg dan nilai RIN lebih dari 7. Kata Kunci: analisis RNA, embrio somatic, kalus, kelapa sawit, kualitas RNA 
METODE EKSTRAKSI DNA TANAMAN TANPA PRESIPITASI ETANOL UNTUK KEGIATAN POLYMERASE CHAIN REACTION (PCR) Nugroho, Kristianto; Terryana, Rerenstradika Tizar; Reflinur, .; Lestari, Puji
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.537 KB) | DOI: 10.29122/jbbi.v6i1.3082

Abstract

A Simplified Plant DNA Extraction Protocol without Ethanol Precipitation for Polymerase Chain Reaction (PCR) Activities ABSTRACTMolecular-based research in agriculture includes DNA extraction stage involving DNA precipitation using ethanol or isopropanol which tends to take a long time. The purpose of this study was to obtain a plant DNA extraction method for Polymerase Chain Reaction (PCR) activities without going through the ethanol precipitation stage. Five important agricultural commodity crops, namely rice, corn, soybeans, chilies, and shallots were extracted by DNA using the modified Doyle and Doyle method. After the extraction phase using chloroform and isoamil alcohol solvents, the supernatant obtained was not precipitated using ethanol but was directly diluted and used as a template in PCR activities using two pairs of Simple Sequence Repeat (SSR) markers. The results showed that all samples could be well amplified, and amplicon tape visualized in both 1% agarose gel and 6% polyacrylamide gel were clearly visible. This method could save time and material, and reduce the dependence on liquid nitrogen. But this method is still limited to PCR requirements only, and cannot be used for activities that require high quality and quantity of DNA such as Next Generation Sequencing (NGS), digestion, and hybridization.Keywords: DNA extraction, ethanol precipitation, liquid nitrogen, PCR, SSR,  ABSTRAKPenelitian berbasis molekuler pada bidang pertanian mencakup tahapan ekstraksi DNA yang melibatkan presipitasi DNA menggunakan etanol atau isopropanol yang cenderung memakan waktu lama. Tujuan penelitian ini adalah untuk memperoleh metode ekstraksi DNA tanaman untuk kegiatan Polymerase Chain Reaction (PCR) tanpa melalui tahapan presipitasi etanol. Lima tanaman komoditas pertanian penting yaitu padi, jagung, kedelai, cabai, dan bawang merah diekstraksi DNA-nya menggunakan metode Doyle and Doyle yang dimodifikasi. Setelah tahap ekstraksi menggunakan pelarut kloroform dan isoamil alkohol, supernatan yang terbentuk tidak dipresipistasi menggunakan etanol melainkan langsung diencerkan dan digunakan sebagai template dalam kegiatan PCR menggunakan dua pasang marka Simple Sequence Repeat (SSR). Hasil menunjukkan bahwa seluruh sampel dapat teramplifikasi dengan baik serta pita hasil amplikon yang tervisualisasi baik pada gel agarosa 1% maupun gel poliakrilamid 6% terlihat jelas. Metode ini dapat menghemat waktu dan bahan serta mengurangi ketergantungan pemakaian nitrogen cair. Tetapi metode ini masih terbatas hanya untuk kebutuhan PCR saja dan tidak dapat digunakan untuk kegiatan yang membutuhkan DNA dengan kualitas serta kuantitas tinggi seperti Next Generation Sequencing (NGS), digesti, maupun hibridisasi.Kata Kunci: ekstraksi DNA, nitrogen cair, PCR, presipitasi etanol, SSR
Front Cover JBBI Vol 6, No 1, June 2019 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (885.549 KB) | DOI: 10.29122/jbbi.v6i1.3704

Abstract

EKSTRAK KAYU TEGERAN (Cudrania javanensis Trécul) SEBAGAI ANTI JAMUR Peniophora sp. Darsih, Cici; Ilyas, Muhammad; Rosyida, Vita Taufika; Pratiwi, Diah; Indrianingsih, A. Wheni; Hernawan, .; Apriyana, Wuri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.684 KB) | DOI: 10.29122/jbbi.v6i1.3168

Abstract

Antifungal Activity of Tegeran Wood (Cudrania javanensis Trécul) Extracts against Fungus Peniophora sp.  ABSTRACTThe fungus Peniophora sp. can degrade natural dyes, causing discoloration on batik cloth. This study was aimed to evaluate the antifungal activity of tegeran wood (Cudrania javanensis) extracts against Peniophora sp. isolated from the aqueous extract of mahogany (Swietenia mahagoni) bark. Aqueous and methanol extraction procedures gave tegeran wood extracts whose concentrations were then varied into 100, 250 and 500 ppm. Phytochemical analysis of the extracts were carried out, and the results showed that the average values of the total polysaccharides and terpenoids of both aqueous and methanol extracts did not differ significantly (p<0.05), whereas those of polyphenols did (p<0.05). The total polyphenols of the methanol extract (484.723 mg GAE/g) was higher than that of the aqueous extract (389.903 mg GAE/g). Results showed that the methanol extract of tegeran wood showed higher antifungal activity against Peniophora sp. than the aqueous extract, and was also higher than ketoconazole control (100 ppm). The minimum inhibitory concentration of the methanol extract against Peniophora sp. growth was of 500 ppm with the antifungal activity value (AFA) of 76.30%.Keywords: antifungal activity, Cudrania javanensis, Peniophora sp., Swietenia mahagoni, wood extract ABSTRAKJamur Peniophora sp. dapat mendegradasi zat warna alam sehingga warna yang dihasilkan pada kain batik lebih pudar. Penelitian ini dilakukan untuk mempelajari aktivitas anti jamur dari ekstrak kayu tegeran (Cudrania javanensis) terhadap Peniophora sp. yang diisolasi dari ekstrak air kulit mahoni (Swietenia mahagoni). Ekstraksi menggunakan metanol dan air, menghasilkan ekstrak kayu tegeran, yang kemudian konsentrasinya divariasi menjadi 100, 250 dan 500 ppm. Analisis fitokimia ekstrak kayu tegeran dilakukan, dan hasilnya menunjukkan bahwa nilai rata-rata total polisakarida dan terpenoid ekstrak metanol dan air kayu tegeran tidak berbeda nyata (p<0,05). Sedangkan total polifenol kedua ekstrak berbeda nyata (p<0,05). Total polifenol ekstrak metanol kayu tegeran (484,723 mg GAE/g) lebih besar dibandingkan dengan ekstrak air (389,903 mg GAE/g). Hasil menunjukkan bahwa aktivitas anti jamur ekstrak metanol lebih baik dibandingkan dengan ekstrak air kayu tegeran, dan juga lebih tinggi dibandingkan dengan kontrol ketoconazole (100 ppm). Konsentrasi minimum ekstrak metanol kayu tegeran yang dapat menghambat pertumbuhan jamur Peniophora sp. adalah 500 ppm dengan nilai penghambatan (AFA) sebesar 76,30%.Kata Kunci: aktivitas anti jamur, Cudrania javanensis, ekstrak kayu, Peniophora sp., Swietenia mahagoni
MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT Davallia denticulata dan Davallia trichomanoides Meliza, Rezika; Chikmawati, Tatik; Sulistijorini, Sulistijorini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.519 KB) | DOI: 10.29122/jbbi.v6i1.2607

Abstract

Spore Morphology and Gametophyte Development of Davallia denticulata and Davallia trichomanoidesABSTRACTDavallia denticulata and D. trichomanoides are two attractive and decorative fern species for ornamental. Spore morphology has an important role in fern taxonomy, while media composition has important role in the growth and development of their gametophytes. Such information on the two fern species was lacking. Therefore, this study aimed to reveal the information of the spore morphology and gametophyte developmental stages of D. denticulata and D. trichomanoides on three different media. The spores were collected from Bogor, West Java. The spores were sown in three sterile media. Spore morphology and gametophyte development were observed under a stereoscopic microscope. Both gametophyte species reached their mature stage at 25 weeks after planting on the different media compositions. D. denticulata showed the best gametophyte development, and formed mature gametophytes on the media of vermiculite, sphagnum moss, and perlite, while D. trichomanoides grew best into maturity stage on the media containing vermiculite, and sphagnum moss. Thus, the presence of sphagnum moss in the media is an important material for the growth and development of Davallia gametophyte.Keywords: Davallia, development, gametophyte, growth, media  ABSTRAKDavallia denticulata dan D. trichomanoides merupakan dua spesies tumbuhan paku yang menarik dan indah untuk tanaman hias. Morfologi spora memiliki arti penting dalam taksonomi tumbuhan paku, sedangkan komposisi media berperan penting untuk pertumbuhan dan perkembangan gametofitnya. Informasi seputar hal ini terkait dua spesies tumbuhan paku tersebut belumlah ada. Penelitian ini bertujuan untuk mengungkap informasi mengenai ciri morfologi spora dan tahapan perkembangan gametofit D. denticulata dan D. trichomanoides pada tiga komposisi media berbeda. Pengambilan spora dilakukan di Bogor, Jawa Barat. Spora ditumbuhkan pada tiga media steril. Morfologi spora dan perkembangan gametofit diamati menggunakan mikroskop stereo. Kedua spesies memiliki waktu perkembangan terbaik untuk mencapai tahap gametofit dewasa yaitu 25 minggu pada komposisi media yang berbeda. D. denticulata berkembang dengan baik, dan membentuk gametofit dewasa pada media vermiculite, lumut sphagnum, dan perlite. D. trichomanoides berkembang hingga tahap gametofit dewasa dengan baik pada media vermiculite, dan lumut sphagnum. Dengan demikian keberadaan lumut sphagnum pada media sangat penting untuk pertumbuhan dan perkembangan gametofit Davallia.Kata Kunci: Davallia, gametofit, media, perkembangan, pertumbuhan
SKRINING DAN IDENTIFIKASI MIKROBA LIGNINOLITIK PADA PENGOMPOSAN ALAMI TANDAN KOSONG KELAPA SAWIT Rupaedah, Bedah; Purwoko, Devit; Safarrida, Anna; Tajuddin, Teuku; Wahid, Abdul; Sugianto, Mahmud; Sudjai, Imam; Suyono, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (713.706 KB) | DOI: 10.29122/jbbi.v6i1.3237

Abstract

Screening and Identification of Ligninolytic Microbes in the Natural Decomposition of Oil Palm Empty Fruit Bunch  ABSTRACTOPEFB (oil palm empty fruit bunch)could potentially be utilized as organic fertilizer or animal feed through composting. Information on microorganisms that play important roles in the natural decomposition of OPEFB is to date not much known yet. This research was aimed to obtain and, subsequently, to molecularly identify lignin-degrading microbial isolates responsible for naturally decomposing OPEFB in the Oil Plant Plantation and Palm Oil Refinery Plant, PTPN VIII Cikasungka, Bogor. Screening for active lignin-degrading isolates was carried out on 17 naturally decomposing OPEFB samples. A total of 19 isolates of fungi and 80 isolates of bacteria were obtained. Ligninolytic activity was measured by Sundman and Nase testing methods. Ligninolytic activity was found on 13 fungal isolates and 15 bacterial isolates. The active isolates were subsequently identified molecularly based on ITS sequence in the ribosome DNA area for fungi and in 16S rRNA genes for bacteria. The results showed that the lignin-degrading microorganisms obtained consisted of 5 bacterial isolates from the genus Bacillus and 3 fungal isolates from the genus Rhizopus and Aspergillus. Keywords: composting, lignin, microbes, OPEFB, 16S rRNA ABSTRAKTKKS (tandan kosong kelapa sawit) berpotensi dimanfaatkan sebagai pupuk organik atau pakan ternak dengan cara pengomposan. Informasi mikroba yang berperan dalam pengomposan alami TKKS hingga saat ini belum banyak diketahui. Penelitian ini bertujuan mendapatkan isolat mikroba pendegradasi lignin dalam pengomposan alami TKKS asal Perkebunan dan Pabrik Pemerasan Kelapa Sawit, PTPN VIII Cikasungka, Bogor, serta mengidentifikasi mikroba tersebut secara molekuler. Skrining mikroba aktif pendegradasi lignin dilakukan terhadap 17 sampel TKKS yang sudah lapuk secara alami. Sebanyak 19 isolat jamur dan 80 isolat bakteri telah dihasilkan. Aktivitas ligninolitik diukur dengan metode pengujian Sundman dan Nase. Isolat jamur yang memiliki aktivitas ligninolitik sebanyak 13 isolat, sedangkan bakteri sebanyak 15 isolat. Isolat-isolat aktif tersebut selanjutnya diidentifikasi secara molekuler berdasarkan pada sekuen ITS di daerah DNA ribosom untuk jamur dan menggunakan gen 16S rRNA untuk bakteri. Hasil menunjukkan bahwa 5 isolat bakteri yang memiliki kemampuan mendegradasi lignin berasal dari genus Bacillus, sedangkan 3 isolat jamur pendegradasi lignin berasal dari genus Rhizopus dan Aspergillus Kata Kunci: lignin, mikroba, pengomposan, TKKS, 16S rRNA 
ISOLASI KHAMIR DARI BATANG TANAMAN TEBU DAN IDENTIFIKASINYA BERDASARKAN SEKUENS INTERNAL TRANSCRIBED SPACER Anggraini, Ika; Ferniah, Rejeki Siti; Kusdiyantini, Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1938.168 KB) | DOI: 10.29122/jbbi.v6i1.3276

Abstract

Isolation of Yeasts from Sugarcane Stems and Their Identification Based on Internal Transcribed Spacer Sequences ABSTRACTFermentative yeasts used in food, health, and energy industries need to be explored to discover their potential. The purpose of this study was to obtain fermentative yeast isolates from sugarcane stems and subsequently to undertake morphological, biochemical, and molecular identification. The isolation of epiphytic and endophytic yeasts was carried out by spread plate method using sugarcane soak water and sugarcane juice on potato dextrose agar (PDA) and yeast-glucose-peptone (YGP) agar media. Morphological identification was based on macroscopic and microscopic observations. Biochemical identification was performed using carbohydrate fermentation and 50%-glucose media tests. Selected isolates were identified molecularly using Internal Transcribed Spacer (ITS). Seven yeast isolates were obtained, of which isolate Ed 1B was selected. Isolate ED 1B was of round colonies, creamy white colour, shiny, embossed, and wavy appearance, ovoid cell shape with a cell diameter of 4.74 µm. It had budding cells, was able to ferment glucose and sucrose (but not lactose), and grew on 50 %-glucose media. Results of BLAST showed that isolates Ed 1B had 99% homology with Kodamaea ohmeri.Keywords: isolation, ITS, molecular identification, Saccharum officinarum L., yeast ABSTRAKKhamir fermentatif yang digunakan dalam industri pangan, kesehatan dan energi perlu dieksplorasi untuk mengetahui potensinya. Tujuan penelitian ini adalah untuk memperoleh isolat khamir fermentatif dari batang tebu dan untuk kemudian diidentifikasi secara morfologi, biokimia dan molekuler. Isolasi khamir epifit dan endofit dilakukan dengan metode cawan sebar dari air rendaman tebu dan jus tebu pada media potato dextrose agar (PDA) dan yeast-glucose-peptone (YGP). Identifikasi morfologi berdasarkan pengamatan makroskopis dan mikroskopis. Identifikasi biokimia menggunakan uji fermentasi karbohidrat dan uji media glukosa 50%. Isolat terpilih diidentifikasi molekuler menggunakan Internal Transcribed Spacer (ITS). Hasil isolasi memperoleh 7 isolat khamir. Satu isolat terpilih (Ed 1B) didapatkan dan memiliki ciri-ciri koloni bulat, putih krem, mengkilap, timbul, bergelombang, bentuk sel ovoid dengan diameter sel 4,74 µm, memiliki budding cell, mampu memfermentasi glukosa dan sukrosa, tidak memfermentasi laktosa, serta tumbuh pada media glukosa 50%. Hasil BLAST menunjukkan bahwa isolat Ed 1B memiliki homologi 99% dengan Kodamaea ohmeri.Kata Kunci: identifikasi molekuler, isolasi, ITS, khamir, Saccharum officinarum L.
MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT Davallia denticulata dan Davallia trichomanoides Rezika Meliza; Tatik Chikmawati; Sulistijorini Sulistijorini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.519 KB) | DOI: 10.29122/jbbi.v6i1.2607

Abstract

Spore Morphology and Gametophyte Development of Davallia denticulata and Davallia trichomanoidesABSTRACTDavallia denticulata and D. trichomanoides are two attractive and decorative fern species for ornamental. Spore morphology has an important role in fern taxonomy, while media composition has important role in the growth and development of their gametophytes. Such information on the two fern species was lacking. Therefore, this study aimed to reveal the information of the spore morphology and gametophyte developmental stages of D. denticulata and D. trichomanoides on three different media. The spores were collected from Bogor, West Java. The spores were sown in three sterile media. Spore morphology and gametophyte development were observed under a stereoscopic microscope. Both gametophyte species reached their mature stage at 25 weeks after planting on the different media compositions. D. denticulata showed the best gametophyte development, and formed mature gametophytes on the media of vermiculite, sphagnum moss, and perlite, while D. trichomanoides grew best into maturity stage on the media containing vermiculite, and sphagnum moss. Thus, the presence of sphagnum moss in the media is an important material for the growth and development of Davallia gametophyte.Keywords: Davallia, development, gametophyte, growth, media  ABSTRAKDavallia denticulata dan D. trichomanoides merupakan dua spesies tumbuhan paku yang menarik dan indah untuk tanaman hias. Morfologi spora memiliki arti penting dalam taksonomi tumbuhan paku, sedangkan komposisi media berperan penting untuk pertumbuhan dan perkembangan gametofitnya. Informasi seputar hal ini terkait dua spesies tumbuhan paku tersebut belumlah ada. Penelitian ini bertujuan untuk mengungkap informasi mengenai ciri morfologi spora dan tahapan perkembangan gametofit D. denticulata dan D. trichomanoides pada tiga komposisi media berbeda. Pengambilan spora dilakukan di Bogor, Jawa Barat. Spora ditumbuhkan pada tiga media steril. Morfologi spora dan perkembangan gametofit diamati menggunakan mikroskop stereo. Kedua spesies memiliki waktu perkembangan terbaik untuk mencapai tahap gametofit dewasa yaitu 25 minggu pada komposisi media yang berbeda. D. denticulata berkembang dengan baik, dan membentuk gametofit dewasa pada media vermiculite, lumut sphagnum, dan perlite. D. trichomanoides berkembang hingga tahap gametofit dewasa dengan baik pada media vermiculite, dan lumut sphagnum. Dengan demikian keberadaan lumut sphagnum pada media sangat penting untuk pertumbuhan dan perkembangan gametofit Davallia.Kata Kunci: Davallia, gametofit, media, perkembangan, pertumbuhan

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