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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Dinamika Kerajinan dan Batik: Majalah Ilmiah
Rosyida, Vita Taufika
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Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Computer Science & IT Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary Other

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EKSTRAK KAYU TEGERAN (Cudrania javanensis Trécul) SEBAGAI ANTI JAMUR Peniophora sp. Darsih, Cici; Ilyas, Muhammad; Rosyida, Vita Taufika; Pratiwi, Diah; Indrianingsih, A. Wheni; Hernawan, .; Apriyana, Wuri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.684 KB) | DOI: 10.29122/jbbi.v6i1.3168

Abstract

Antifungal Activity of Tegeran Wood (Cudrania javanensis Trécul) Extracts against Fungus Peniophora sp.  ABSTRACTThe fungus Peniophora sp. can degrade natural dyes, causing discoloration on batik cloth. This study was aimed to evaluate the antifungal activity of tegeran wood (Cudrania javanensis) extracts against Peniophora sp. isolated from the aqueous extract of mahogany (Swietenia mahagoni) bark. Aqueous and methanol extraction procedures gave tegeran wood extracts whose concentrations were then varied into 100, 250 and 500 ppm. Phytochemical analysis of the extracts were carried out, and the results showed that the average values of the total polysaccharides and terpenoids of both aqueous and methanol extracts did not differ significantly (p<0.05), whereas those of polyphenols did (p<0.05). The total polyphenols of the methanol extract (484.723 mg GAE/g) was higher than that of the aqueous extract (389.903 mg GAE/g). Results showed that the methanol extract of tegeran wood showed higher antifungal activity against Peniophora sp. than the aqueous extract, and was also higher than ketoconazole control (100 ppm). The minimum inhibitory concentration of the methanol extract against Peniophora sp. growth was of 500 ppm with the antifungal activity value (AFA) of 76.30%.Keywords: antifungal activity, Cudrania javanensis, Peniophora sp., Swietenia mahagoni, wood extract ABSTRAKJamur Peniophora sp. dapat mendegradasi zat warna alam sehingga warna yang dihasilkan pada kain batik lebih pudar. Penelitian ini dilakukan untuk mempelajari aktivitas anti jamur dari ekstrak kayu tegeran (Cudrania javanensis) terhadap Peniophora sp. yang diisolasi dari ekstrak air kulit mahoni (Swietenia mahagoni). Ekstraksi menggunakan metanol dan air, menghasilkan ekstrak kayu tegeran, yang kemudian konsentrasinya divariasi menjadi 100, 250 dan 500 ppm. Analisis fitokimia ekstrak kayu tegeran dilakukan, dan hasilnya menunjukkan bahwa nilai rata-rata total polisakarida dan terpenoid ekstrak metanol dan air kayu tegeran tidak berbeda nyata (p<0,05). Sedangkan total polifenol kedua ekstrak berbeda nyata (p<0,05). Total polifenol ekstrak metanol kayu tegeran (484,723 mg GAE/g) lebih besar dibandingkan dengan ekstrak air (389,903 mg GAE/g). Hasil menunjukkan bahwa aktivitas anti jamur ekstrak metanol lebih baik dibandingkan dengan ekstrak air kayu tegeran, dan juga lebih tinggi dibandingkan dengan kontrol ketoconazole (100 ppm). Konsentrasi minimum ekstrak metanol kayu tegeran yang dapat menghambat pertumbuhan jamur Peniophora sp. adalah 500 ppm dengan nilai penghambatan (AFA) sebesar 76,30%.Kata Kunci: aktivitas anti jamur, Cudrania javanensis, ekstrak kayu, Peniophora sp., Swietenia mahagoni
White Rot Fungus (Marasmius sp.) Delignification on Sugarcane Bagasse for Bioethanol Production Darsih, Cici; Wahono, Satriyo Krido; Rosyida, Vita Taufika; Kismurtono, M.
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2015: Proceeding ISETH (International Conference on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.2355

Abstract

Sugarcane bagasse is one of the potential lignocellulose materials for alternative energy especially on the development of bioethanol, the second generation technology. This paper investigates the delignification of sugarcane bagasse by the white rot fungus (Marasmius sp.). Sugarcane bagasse was delignified by fungus; then hydrolyzed by xylane enzyme and fermented by Saccharomyces cerevisiae through simultaneous processes of saccharification and fermentation (SSF). Pretreatment of the sugarcane bagasse was conducted by fungus with various time periods of incubations for 15, 30, and 45 days. SSF of pretreated sugarcane bagasse was conducted for 3, 6, 9, 12, and 15 days. Lignin, cellulose, and hemicellulose were determined by Chesson method. Bagasse structure before and after delignification were determined by Fourier Transform Infrared (FTIR) Spectroscopy. The results showed that the best result of bagasse delignification, containing 16.04% of cellulose, 26.61% of hemicellulose, and 51.89% of cellulose, was on 15 days of incubation. The bioethanol was obtained in 3 days of incubation with bioethanol concentration of 0.85% or 0.4 g/L.
DYEING OF COTTON FABRIC WITH NATURAL DYE FROM CUDRANIA JAVANENSIS USING SOKA LEAVES EXTRACT AS BIOMORDANT Darsih, Cici; Lestari, Dwi Wiji; Pratiwi, Diah; Indrianingsih, A Wheni; Rosyida, Vita Taufika; Apriana, Wuri; Nisa, Khoirun; Hayati, Septi Nur; Handayani, Sri; Wulanjati, Martha Purnami
Dinamika Kerajinan dan Batik: Majalah Ilmiah Vol. 36 No. 2 (2019): Dinamika Kerajinan dan Batik : Majalah Ilmiah
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Industri Kerajinan dan Batik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22322/dkb.v36i2.5446

Abstract

Tegeran wood (Cudrania javanensis) has been used for dyeing process of batik in Indonesian textile small medium scale enterprise. This wood gave soga color when it was mixed with others natural dye. This study was aim to evaluate the caharacteristic, fastness properties, and strength color of dyed cotton fabrics using C. javanensis with bio-mordant soka leaves extract. Extraction of C. javanensis was carried out using water. The post mordant process was carried out by applying alum (KAl(SO4)2•12H2O) solution and soka leaves extract on cotton fabrics with the concentration of 0.5 and 1% b/v. Characteristic of dyed cotton fabrics then was evaluated using Fourier-Transform Infrared (F-TIR) Spectroscopy. The results exhibited that, binding between tegeran wood on cotton using mordant alum and soka leaves extract was similar.The fastness properties both dyed cottons toward light and  washing at 40ºC exhibited poor and very poor values with values of 1-2 and 1. The dry and wet rubbing of dyed cotton using soka leaves extract mordant better than using alum. The soka leaves extract gave yellow and brightness color on cotton.The results suggested that soka leaves extract can be used as mordant substitute the alum mordant and apllied with others natural dyes.
Antifungal Citrus hystrix Extract as Natural Food Preservative Nisa, Khoirun; Herawati, Ervika Rahayu Novita; Nurhayati, Septi; Rosyida, Vita Taufika
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.433 KB) | DOI: 10.11598/btb.0.0.0.1154

Abstract

During their storage, the traditional ready-to-eat food, such as sticky rice cake, is easily contaminated by spoilage pathogens. Hence, this study aims to evaluate the effect of Citrus hystrix extract in reducing spoilage pathogens in sticky rice cake during storage. The experimental sticky rice cake was prepared and formulated with Citrus hystrix extract at varied concentrations of 0.65%, 1.26%, and 1.82% (w/w). Treated samples were stored at room temperature for 28 days and evaluated periodically for their microbial activity (total plate count), thiobarbituric acid reactive substances (TBARS), and sensory analysis. For its antifungal activity, the Citrus hystrix extract was also compared against Penicillium sp. and Aspergillus nidulans prior to formulation. Results exhibited a significant advantage of the added extracts to the sticky rice cake. All extract levels effectively eliminated the spoilage microorganisms and significantly lowered the TBARS values. The physico-chemical properties of sticky rice cake including pH, water activity, and moisture content were equal among all the formulated samples and slightly different at 1.82% (w/w) extract level. Moreover, the addition of Citrus hystrix extract up to 1.82% did not affect the acceptability sensory attributes of the sticky rice cake as compared to the control which had no Citrus hystrix extract (p > 0.05).
Co-Authors Apriana, Wuri Cici Darsih Diah Pratiwi Dwi Wiji Lestari, Dwi Wiji Handayani, Sri Hayati, Septi Nur Herawati, Ervika Rahayu Novita Hernawan, . Indrianingsih, A Wheni Indrianingsih, A. Wheni Khoirun Nisa Kismurtono, M. Martha Purnami Wulanjati, Martha Purnami Muhammad Ilyas Septi Nurhayati Wahono, Satriyo Krido Wuri Apriyana, Wuri