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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 193 Documents

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Utility of Neutrophil Lymphocyte Ratio (NLR) and Platelet Lymphocyte Ratio (PLR) as A Predictor of Mortality in COVİD-19 Riya Sunil; Preethi Chikkanayakanahalli Ramesh; Ashwini Hosahalli Nagaraj; Krupa Kadarappa
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Coronavirus-19 pandemic has stricken our world since December 2019; the disease, first reported in China, is now a pandemic. More than 400 million people have been affected, and 5 million people have succumbed to the disease. Hence, it is the need of the era to find readily available laboratory parameters to assess the mortality chances in these patients. Our study aims to determine the utility of NLR and PLR ratios as a predictor of severity and clinical outcome of COVID-19 patients.100 patients admitted to a tertiary care hospital in Karnataka, India, during the months April to July 2020 were studied. Only patients with a positive RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) report for COVID-19 were included. Demographic data, comorbidities, and mortality status were collected from electronic hospital records. Lab parameters including- Total Count (TC), Absolute count of neutrophils and lymphocytes, platelet count were taken. NLR and PLR were derived from available lab parameters. Patients were categorized into varying severity depending on their SpO2 levels at admission. Neutrophil count (P=0.001) and NLR (P=0.002) were associated with an increased risk of mortality and disease severity. An increase in PLR ratio (P=0.05) shows a mild association with mortality but not with disease severity (P=0.096). In contrast, comorbidities, increasing age, and gender did not show any statistical significance for mortality. The presence of statistical significance concerning NLR and PLR should be utilized as an aid by clinicians to assess disease severity and chances of mortality. As new variants of the disease are uprising and a single therapeutic measure is not available currently for the treatment of COVID-19, clinicians should be well informed about how to monitor the disease in a cost-effective and easily accessible way to reduce the disease mortality and morbidity.

Assessment of the RIPASA Score in the Diagnosis of Acute Appendicitis in Khartoum State Hospitals, Sudan Mohammed Hashim Yousif Ahmed; Aamir Abdallah Hamza; Alawia Mohammed Mansour; Khalid Yousif Abuaagla
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Appendectomy is a common cause of emergency hospitalization, and appendectomy is one of the most common emergency procedures performed in modern medicine. Although it is a common problem, acute appendicitis remains a complex condition to diagnose, especially in the young, the elderly, and women of childbearing age. The aim was to evaluate the accuracy of the RIPASA score for patients with right iliac fossa pain (RIF) and suspected appendicitis. This study is an observational, analytic, and prospective cohort study conducted for one year. All patients suspected of having acute appendicitis and who underwent appendectomy were included in this study. All resected appendages were sent for histological examination. RIPASA scores were calculated for all patients. The study results in the 105 patients studied, 60 (57.1%) were predominantly male. The M/F ratio is 1.3/1. Most of the 98 patients (93.3%) were 40 years old. Most patients had symptoms within 48 hours 79 (75.2%). The most common symptom was anorexia (83.8%). All patients had RIF tenderness (100%). 95 (90.5%) patients had a score >7.5. On gross examination, three patients (2.9%) had a non-inflammatory appendix, 83 patients (79.0%) had a simple inflammatory appendix, the perforated appendix was found in 9 patients (8.6%), appendix mass was found in 6 patients (5,7%), and only four patients (3.8%) had an appendiceal abscess. The appendix was histologically inflamed in 69 patients (65.7%). The sensitivity of the RIPASA scoring system in our study was 69.5%, and the specificity was 70%. The diagnostic accuracy was calculated and was 69.5%. Our study's positive and negative predictive values (PPV, NPV) were 95.7% and 19.4%, respectively. Research conclusion RIPASA value of 7.5 is beneficial and easy to use. Therefore, surgeons can use it as a diagnostic guide for acute appendicitis to reduce the rate of misdiagnosis.

Clinicopathological Study of Mucormycosis in Post Covid Patients, an Epidemic in Pandemic Divya Pujari Jayakeerthy; Pradeep Kumar Reddy Mereddy; Adicherla Govardhan; Seema Bijjaragi
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Mucormycosis is an angio-invasive disease caused by saprophytic fungi of the order Mucorales. Mucormycosis has been established and recognized as a complication of the SARS-CoV-2 infection. Mucormycosis, well known as "black fungus" is creating an epidemic within a global pandemic. The use of different dose regimens, prominent steroids, and multiple comorbidities like Diabetes Mellitus and post-COVID could predispose to mucormycosis. The aim of the study was to study the increase in cases and morphological features of mucormycosis infection in hospitalized post-COVID patients. This prospective study was done for four months, from May 1st, 2021, to August 31st, 2021. All the suspected cases of mucormycosis specimens sent to the Department of Pathology, J.J.M. Medical College, Davangere, for histopathological diagnosis were considered. For microscopic examination, the collected samples were fixed in 10% formalin solution, processed, and stained with Haematoxylin and Eosin. Periodic Acid-Schiff special staining was done. Thirty cases of mucormycosis were collected from patients who had recovered from COVID-19. Mean age 63±12 (37-80) years with Male:Female ratio being 1.3:1. The mean duration between diagnosis of COVID-19 and development of symptoms of mucor was 23.5±10 (7–60) days. Rhino-orbital mucormycosis was the most typical presentation. Out of 30 cases, 28 cases (93.3%) were Type 2 Diabetes Mellitus, and 2 cases (6.6%)were nondiabetic. All patients received corticosteroids for the treatment of COVID-19. Trinity of COVID -19, Diabetes Mellitus, and Corticosteroid treatment was significant for the rise in mucormycosis cases. Mucormycosis is an invasive fungal infection and had seen a sudden surge during the second wave of COVID-19. In our study, most patients had a history of diabetes, and corticosteroid therapy with rhino-orbital mucormycosis was the most typical presentation. As the severity of the necrosis increases, the presence of fungal hyphae also increases, which leads to extensive destruction of tissue. Extra vigilance in immunosuppressed patients helps in reducing the morbidity and mortality due to mucormycosis in Post COVID era.

Effectiveness of Xylene and Mineral Oil in DNA Extraction from Formalin-fixed Paraffin-embedded Diffuse Astrocytic Tumor Edward Jaya Hadi; Hasrayati Agustina; Hermin Aminah Usman
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Formalin-fixed paraffin-embedded (FFPE) tissue is an important source of material for molecular analysis. In the anatomical pathology field, molecular testing is needed in certain tumors, such as astrocytic tumors, to confirm the diagnosis. Extracting DNA from FFPE material is still challenging. The first important step in the extraction process is deparaffinization. This study aims to compare two types of deparaffinization methods. The first method used xylene, and the second one used mineral oil. The results of this study can be used to develop a reliable protocol for DNA extraction from FFPE tissue. DNA from 28 FFPE diffuse astrocytic tumor tissue blocks was extracted. The quantity and A260/A280 ratio of the DNA was measured by spectrophotometer. PCR assays were performed to assess the suitability of extracted DNA for molecular analysis. The results showed that the xylene group has significantly higher DNA concentrations than the mineral oil group (p<0.01). In both groups, average and median A260/A280 scores were between 1.8-2.0. In PCR assay, both groups show a similar result (27 of 28 samples were successfully amplified). In conclusion, xylene and mineral oil can be used effectively as deparaffinization agents. Both methods generated good quality extracted DNA. The advantage of mineral oil is that it is non-toxic and has shorter hands-on time. Xylene is preferable for a small-sized sample because it produces a higher DNA yield.

Significance of D-dimer in Assessment of Severity and Mortality of COVID-19 Patients A Tertiary Care Hospital Study Ashwini Suruthy; Neethu Gowdru Vishwanathan; Seema Bijjaragi
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The outbreak of COVID-19 has brought public health emergency worldwide and has been the biggest challenge to date. This study aims to study the correlation between D-dimer levels with severity and mortality in COVID-19 patients. This study will help us understand the disease's progression and monitor the disease severity and mortality in Covid-19 patients. This retrospective descriptive study was conducted over four months from April 2021- to June 2021 in a tertiary care hospital in Central Karnataka. Demographic details and clinical and laboratory parameters were obtained from electronic hospital records. Inclusion criteria are Patients who were RT-PCR confirmed positive cases were only included in this study. Exclusion criteria are Patients who had cancer, hematologic malignancies, and acute coronary syndrome were all excluded. Results of the research are A total of 75 cases were evaluated and analyzed in this study. Patients were categorized based on severity as mild to moderate and severe. Out of which 50 patients had recovered from the illness, and 25 patients have succumbed to death. Our study revealed that elevated D-dimer levels were significant (p<0.02) with the outcome of the disease, and with the mean level of 929.27 in severe patients and 466.47 in mild-moderate patients, elevated D-dimer were highly significant (p<0.001) with the severity of the disease. Conclusion research detailed investigations of 75 COVID-19 patients suggests that a significant increase in D-dimer levels was associated with mortality and severity of the disease. Therefore, we strongly recommend that the D-dimer be used to screen patients with COVID-19 to evaluate the severity and predict the prognosis and mortality in hospitalized COVID-19 patients during admission and follow-up throughout hospitalization.

Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli Ratih Dewi Dwiyanti; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Dracontomelon dao (Blanco) Merr. & Rofe has antimicrobial potential, but Dracontomelon dao (Blanco) Merr. & Rofe, originating from Kalimantan, Indonesia, has not been tested for its antibacterial properties and secondary metabolite content. This study aimed to analyze the secondary metabolite content of various Dracontomelon dao (Blanco) Merr leaf extracts. & Rofe and their antibacterial effectiveness against Escherichia coli. The study used 96% ethanol extract, ethyl acetate, petroleum ether, and chloroform by maceration simplicia leaves. Dracontomelon dao (Blanco) Merr. & Rofe is from South Kalimantan, Indonesia. Antibacterial testing was carried out using the agar diffusion method, the Minimum Inhibitory Concentration (MIC) method, and the Minimum Bactericidal Concentration (MBC) method. The results showed that the triterpenoid content was the highest in all extracts, namely ethanol extract 669.8 ± 2,000 mg/ml, ethyl acetate 90 ± 7,638 mg/ml, petroleum ether 792, 800 ± 4,583 mg/ml, chloroform 615, 467 ± 0,577 mg/ml. ml. The antibacterial test using the well diffusion method showed the best inhibition of bacterial growth in ethanol extract, with the largest inhibition zone of 24 mm at a concentration of 500 mg/ml. The study's conclusion was the extract of ethanol, ethyl acetate, petroleum ether and chloroform from the leaves of Dracontomelon dao (Blanco) Merr. & Rofe contains the same five secondary metabolites: flavonoids, alkaloids, saponins, tannins and triterpenoids. All types of extracts showed varying abilities to inhibit the growth of Escherichia coli. Further research is needed on the antibacterial properties of Dracontomelon dao (Blanco) Merr extract. & Rofe, especially on the active substance triterpenoid, which is the largest content in all types of extracts.

Survival and Clinicopathological Features of Cervical Carcinoma Patients with Radiation Therapy Yahya Irwanto; Aliesya Patricia Wulandari; Ineke Permatasari; Ainun Ganisia; Lilik Zuhriyah; I Wayan Agung Indrawan; Sutrisno .
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Cervical cancer has a high incidence with a low survival rate. The clinicopathological profile and risk factors cause this. Most cervical cancer sufferers are late in realizing this, contributing to the severity suffered. Radiation has a very important role because it is a therapy that can be done in cervical cancer patients who have experienced an advanced level of severity. This study aims to determine the survival and influence of the clinicopathological and risk factors of radiation patients as a consideration in selecting effective therapy to increase the survival rate of cervical cancer patients. The benefit of this research is to help evaluate cervical cancer management procedures with radiation therapy applied in many hospitals in Indonesia to increase the success of therapy for cervical cancer. An analytical observational study with a retrospective cohort type using medical record data with samples of all patients diagnosed with cervical cancer who received radiation therapy from January to December 2017. Samples in this study were 111 patients. This study revealed that the five-year survival rate of cervical cancer patients was only 53.1%. There was an influence on the clinicopathological stage (p<0.000), histopathology (p<0.000), lymph node status (p<0.000), degree of differentiation (p<0.001), and risk factors for age (p<0.038), parity (p<0.001), education (0.022), marital status (0.001), contraceptive use (p<0.000), and BMI (p<0.000) which resulted in a log-rank p-value <0.05, so it was concluded that there was an effect between these variables on cervical carcinoma survival rate. Multivariate, it was found that radiation, histopathology, lymph node status, contraception, and BMI had a significant effect (p<0.005) on the survival rate of cervical cancer patients. The results of this study indicate that the survival rate of cervical cancer patients is still low, and there is a clinicopathological and risk factors that influence the survival rate of cervical cancer patients.

Effect of Papaya Leaf Extract Gel (Carica papaya) on Incision Wound Healing in Rattus norvegicus Iin Tri Marlinawati; Siti Nurhidayah; Sanarto Santoso; Yahya Irwanto
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Carica papaya is one kind of herbal plant that can help the wound healing process. Scientifically, the leaves of papaya are proven to have anti-inflammatory effects. Flavonoid, alkaloid, tannin, and saponin compounds in papaya leaves have antimicrobial and antioxidant properties to accelerate the re-epithelization and cell migration needed for wound healing. This research aims to determine the effect of papaya leaf extract gel (Carica papaya) on the healing of incision wounds in Rattus norvegicus based on changes in wound morphology (signs of redness, edema, pus, granulation, moisture, and wound length). This research used female Rattus norvegicus aged 12-16 weeks weighing 180-200 grams, divided into five groups (NaCl 0,9%, povidone-iodine, papaya leaf extract gel with concentrations of 10%, 20%, and 30%). Incision wounds on the back of the rat with a length of 2 cm and depth of 1 cm. The normality test used the Shapiro-Wilk test, and the homogeneity test used the Levene test. The research data were not normally distributed nor homogeneous, so they were analyzed using the Kruskal Wallis and Mann-Whitney tests. The results showed that the extract of papaya leaves with concentrations of 10%, 20%, and 30% had significant differences in positive and negative control groups in the wound healing process with the differences in redness (p=0.038), granulation tissue (p=0.039), moistures (0,48), and wound length (p=0,049). It can be concluded that papaya leaf extract gel (Carica papaya) is effective in accelerating the healing process of incision wounds in Rattus norwegicus at concentrations of 10%, 20%, and 30%, so for further research, it is hoped that a histopathological examination can be carried out so that changes that occur in collagen, neutrophile cells, monocytes, and lymphocytes are seen.

Effect of Time and Temperature Variables on Prothrombin Time and International Normalized Ratio of Plasma in Patients in Gampaha, Sri Lanka Vıraj Ranasınghe; Sameera Maduranga Jayasundara; Sanjaya De Sılva; Faiz Marikar; Randıka Wımalasırı Yapa
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Generating accurate results for Prothrombin time (PT) and International Normalized Ratio (INR), PT/INR test is paramount in treatment monitoring. The diagnosis process measures how long it takes blood to clot. The main objective of this study was to determine the effect of storage time and temperature variables on PT and INR results in patients attending Hematologic clinics in Sri Lanka. This cross-sectional study was conducted during June and July 2021. PT was measured in the plasma samples, stored at different temperatures at baseline (0 hours), and then after 12 hours, 18 hours, and 24 hours after specimen collection. INR was calculated from each result, and the deviation percentage of PT and INR was calculated compared to the baseline result (0 hours). Then PT and INR percentage deviation is grouped as <10% and >10% to find biologically significant variations while paired t-test was performed to find statistical significance using GraphPad prism 8.4.3. At refrigerated temperature, both PT and INR results showed>10% variation once kept 24 hours, and this variation was statistically significant (p<0.01, Wilcoxon matched paired sign ranked test). The plasma specimens kept at laboratory temperature and specimen collection room temperature for 18 hours indicated >10% variation of PT and INR values, while these variations were significant at the same criteria above. These results suggest that the separated plasma could only be recommended to test up to 12 hours. Therefore, the laboratories that operate in the same climatic conditions can now use these results to plan the specimen collection accordingly.

Potential Benefit of Flavonoid in Papaya Leave Gel in Neutrophil, Angiogenesis, and Wound size in Rattus Norvegicus Siti Nurhidayah; Iin Tri Marlinawati; Sri Winarsih; Tatit Nurseta
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

An incision wound is the destruction of several cells in the skin area that has experienced an incision. Several factors influencing wound healing are neutrophils as causative agents of inflammation, angiogenesis, and wound closure. These three factors should undergo physiologically to obtain the optimal healing process. Currently, herbal products are preferred because they have lower side effects than synthetic ones. Papaya leaves contain flavonoids with various functions, including wound closure and anti-inflammatory and antioxidant effects. Topical intervention, especially gel, is good for healing because it can keep the wound moist. This research aimed to determine the effect of the administration of papaya leaf gel on wound closure, neutrophil, and angiogenesis. Thirty white rats were used as the model animals in a randomized experimental study divided into five groups comprising negative control (0,9% NaCl), positive control (10% Povidone-iodine), and three intervention groups treated with gel of Carica papaya leaves at a dosage 10%, 20%, and 30%. All groups were topically administered treatment once a day on the dorsal surface for seven days. Carica papaya leaves contain flavonoids, saponin, tannin, and alkaloids qualitatively and 7,734 mg/kg of the flavonoid quantitatively. There were no significant differences in wound size in all three groups on three days, but Carica papaya leave gel had a superior effect compared to 10% PI and 0,9% NaCl in the wound closure process. There were significant differences in all groups in neutrophil and angiogenesis. Nevertheless, 20% papaya leave gel showed the lowest number of neutrophils and had a superior effect on improving the new blood vessels significantly compared to negative and positive groups.

Page 13 of 20 | Total Record : 193

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Ricko Dharmadi Utama

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Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

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Abstract

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Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA