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INDONESIA
Medical Laboratory Technology Journal
Published by Poltekkes Kemenkes Banjarmasin
ISSN : -     EISSN : 24610879     DOI : https://doi.org/10.31964/mltj
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 193 Documents
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Hematological Effects of Vigna unguiculata subsp. cylindrica in Anemic Mice: An Experimental Study Norsiah, Wahdah; Thuraidah, Anny; Herlina, Tini Elyn
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.687

Abstract

Anemia is a global health problem that impairs cognitive function, physical performance, and maternal–child health. This study aimed to evaluate the hematological effects of Vigna unguiculata subsp. cylindrica (Nagara Bean) as a potential functional food in sodium nitrite (NaNO₂)-induced anemic mice. Thirty-three male BALB/c mice (8–12 weeks, 25–30 g) were randomly assigned into six groups: normal control (K), negative control (K−, anemia-induced), positive control (K+, iron-supplemented), and three treatment groups (P1, P2, P3) that received Nagara Bean-based feed for 1, 2, and 3 weeks, respectively. Hemoglobin (Hb), hematocrit (Hct), and red blood cell (RBC) levels were measured using Point-of-Care Testing (POCT) and manual cell counting. The results showed increases in Hb, Hct, and RBC across the treatment groups; however, statistical analysis indicated that these improvements were primarily associated with the duration of feed administration rather than treatment type. The greatest hematological improvement was observed after two weeks of feeding (P2). These findings indicate that Nagara Bean supplementation supports hematological recovery in anemic mice. The results suggest that Vigna unguiculata subsp. cylindrica has potential as a natural, affordable, and locally available source of bioavailable iron for anemia prevention and dietary intervention programs.
Salıva pH, Carıes and OHI-S Score in Stuntıng Pre-School Chıldren in The Swamp Area Utami, Naning Kisworo; Andrestian, Meilla Dwi; Insana, Aima
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.688

Abstract

Stunting remains a major global health issue and is associated with impaired physical and cognitive development, as well as increased susceptibility to oral diseases such as dental caries. Environmental factors, including acidic peatland water, may further aggravate oral health conditions among children living in swamp areas. This study aimed to analyze the relationship between salivary pH, dental caries severity (def-t index), and oral hygiene status (OHI-S) in preschool children with stunting living in peatland regions of South Kalimantan, Indonesia. An analytical observational study with a cross-sectional design was conducted involving 32 preschool children aged 2–5 years residing in the working area of the Martapura Timur Public Health Center. Data collection included clinical examination of caries using the WHO criteria, salivary pH measurement with a Krisbow digital pH meter, and assessment of oral hygiene using the Simplified Oral Hygiene Index (OHI-S). Statistical analysis was performed using the Spearman correlation test. The results showed that salivary pH ranged from 5.5 to 7.0, def-t scores ranged from 2 to 8 (mean = 5.34 ± 1.78), and OHI-S scores were 0.00 in 22 children (68.7%) and >0.00 in 10 children (31.3%). A significant association was found between salivary pH and dental caries severity (p = 0.005; α = 0.05), indicating that lower salivary pH is associated with higher caries prevalence. In conclusion, stunted preschool children in peatland areas exhibited acidic salivary conditions and a high caries burden despite relatively good oral hygiene status. These findings highlight the importance of integrating nutritional rehabilitation and oral health education programs to reduce the risk of dental caries in children living in environmentally vulnerable swamp regions.
Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Abstract

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.

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