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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 193 Documents

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Animal Models with Metabolic Syndrome Markers Induced by High Fat Diet and Fructose Larantika Hidayati; Agung Dwi Wahyu Widodo; Boerhan Hidayat
Medical Laboratory Technology Journal Vol. 6 No. 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Metabolic syndrome is lipid and non-lipid metabolism disorder due to the association of several factors such as physiological, clinical, biochemical, and interrelated factors. People with metabolic syndrome can be diagnosed by fulfilling 3 of 5 criteria, including obesity and increased waist circumference, increased TG levels, increased blood pressure, hyperglycemia, and increased High-Density Lipoprotein (HDL) serum. The high-fat diet disrupts tissue lipid metabolism, so insulin resistance occurs due to lipotoxicity. Besides, some studies use a combination of mixtures (fructose, sucrose) and fat-rich food components to build metabolic characteristics in mice that affect human characteristics. The purpose of this study was to make an animal model with a metabolic syndrome marker induced by the High Fat Diet (HFD) consisting of pork oil and chicken egg yolk, as well as fructose from simple and economical ingredients. This study was an experimental study using experimental animals of male Rattus norvegicus strain wistar, which were grouped into three random treatment groups, namely the control group, HFD group, and High Fat Diet Fructose (HFDF) group. The number of samples used was 27 rats, with nine rats in each group. The animal was induced for four weeks, then measured levels of FPG, HD, and TG. From the research that has been done, it is found that there are significant differences in levels of Fasting Plasma Glucose (FPG), High-Density Lipoprotein (HDL), and Triglycerides (TG) (p

Hemoglobin Levels on Trichuris trichiura Infection in Children Ni Putu Aryadnyani; Dewi Inderiati; Fauzia Ulfah
Medical Laboratory Technology Journal Vol. 6 No. 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Worm infections often caused by worms transmitted through the soil (Soil-Transmitted Helminths). Types of STH worms include roundworms (Ascaris lumbricoides), whipworms (Trichuris trichiura) and hookworms (Ancylostoma duodenale and Necator Americanus). Worms commonly suffered by school-age children with the most aged between 5-14 years. Worms can affect the intake, digestion, absorption, and metabolism of food, which can result in loss of protein, carbohydrates, fats, and vitamins. Trichuris trichiura and hookworm can cause massive amounts of blood loss and decreased hemoglobin levels. This study aims to determine the relationship of Trichuris trichiura infection with hemoglobin levels in children in the village of East Serang Kilasah, Banten. This research is an analytic survey with a cross-sectional design with 42 respondents. The sampling technique used was purposive sampling. The results of the fecal examination by the Kato-Katz method found 23 children (54.76%) with mild worm levels and 19 children (45.24%) with moderate worm levels. The results of blood tests found 36 children (85.71%) with normal hemoglobin levels and six children (14.79%) with low hemoglobin levels. Based on the results of statistical tests, the results obtained P-value 0.004 (P-value

Biolarvasides of Cananga odorata Flower and Zyzygium polyanthum Leaves Ethanol Extract on Aedes aegypti Larva Nurlailah Nurlailah; Anny Thuraidah
Medical Laboratory Technology Journal Vol. 6 No. 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than α (0.001

Comparative Test of Ret-He Examination in Diagnosis of Iron Deficiency in Pregnant Women Gilang Nugraha; Nur Masruroh; Diyan Wahyu Kurniasari
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The most common cause of anemia is iron deficiency, which found in 50% of anemia cases. There are 66.7% of pregnant women in Indonesia that still lack iron intake. Reticulocyte hemoglobin equivalent (Ret-He) examination as a single parameter is the initial indicator of iron deficiency, considered more sensitive in detecting iron reserves in the body. This study aims to determine the effectivity of the Ret-He examination as a single parameter in establishing the diagnosis of iron deficiency in pregnant women. This research was conducted on 30 pregnant women. The sensitivity, specificity, and predictive value of Ret-He were determined by comparing the results of the biochemical examination of iron profiles. Iron deficiency defined as an abnormal examination result on two or more biochemical parameters, which were SI 425 µg/dL, serum ferritin

Early Relapse After Autologous Stem Cell Transplantation in Multiple Myeloma is Still Prognostic in The Era of Novel Agents Semih Başcı; Tuğçe Nur Yiğenoğlu; Bahar Uncu Ulu; Mehmet Bakırtaş; Derya Şahin; Tahir Darçın; Jale Yıldız; Alparslan Merdin; Nuran Ahu Baysal; Dicle İskender; Merih Kızıl Çakar; Mehmet Sinan Dal; Tuba Hacıbekiroğlu; Fevzi Altuntaş
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Significant improvements in the prognosis of Multiple Myeloma(MM) have recently observed in the era of novel agents. Induction treatment, including new agents followed by conditioning regimen and upfront autologous stem cell transplantation(ASCT), has been accepted as the standard treatment approach for newly diagnosed eligible MM patients. Despite novel agents, upfront ASCT is still superior to conventional chemotherapy alone. Previous studies revealed that the duration between ASCT and relapse had predicted overall survival(OS), and meantime, it was widely used to determine the potential benefit from a second ASCT. However, the majority of the data collected reflects the treatment modalities before novel agents. In this study, we aimed to investigate the impact of post-transplantation early relapse(ER) on survival in the era of novel agents. The results of 155 MM patients that underwent ASCT at our center between January 2010 and May 2018 were analyzed retrospectively. The median follow-up duration was 20 months in the ER group, 27 months in the non-ER group, and 24 months in all patients. 33.3% of patients in the ER group and 71.4% of patients in the non-ER group were alive at the time of analysis. Median OS was 20.77±3.66 months in the ER group and 40.89±4.21 months in the non-ER group. We found a statistically significant relationship between the ER and the poor OS (p:

Increased Monocyte Count is Related to the Development of Atrial Fibrillation in Subjects with Heart Failure Recep Kurt; Anıl Şahin
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Determining subjects with heart failure (HF), who have a high risk of developing atrial fibrillation (AF), is crucial since it is related to an increase in morbidity and mortality. The importance of an increase in inflammatory response cannot be ignored in developing AF in subjects with HF. This study’s goal was to evaluate the relationship between the development of AF and monocyte count, the main components of the inflammatory response, in subjects with HF. Medical data of 158 subjects in total with sinus rhythm, admitted to the emergency department and hospitalized with systolic HF between January 2009 and February 2014, were analyzed prospectively. All the subjects followed up in terms of the development of AF. The subjects separated into two groups according to their rhythm at the end of the follow-up period. Group 1 included subjects who maintained sinus rhythm, while Group 2 included subjects with AF. Echocardiographic findings and laboratory data were collected. No statistically significant difference was determined between the groups concerning age, sex (p=0.056), hypertension, diabetes mellitus, and smoking status. The two groups differed significantly regarding the presence of moderate-to-severe mitral regurgitation (MR) [32 (28%) vs. 22 (51%), p=0.010] and monocyte count [560 (20-3100) vs. 800 (380-1510) μL, p

Stability of Routine Hematology Sample Using The Medonic M-Series Analyzer Eva Ayu Maharani; Dewi Astuti
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Routine hematology tests (Hb, Hct, RBC, WBC, PLT) generally done by automation methods using a hematology analyzer. Ideally, the examinations should be done as soon as possible, although, in some circumstances, it can be delayed. Based on the literature, the sample for routine hematology testing should be processed within 4 – 8 hours of venesection. Some studies revealed that the sample could be stored for up to 48 hours, and it can be influenced by the technology applied to the hematology analyzer. Studies conducted to see the stability of the samples using a hematology analyzer with impedance technology. Tests performed on whole blood samples collected from 12 ostensibly healthy individuals, immediately after collection (fresh sample,

The Effects of Hyperbaric Oxygen Therapy on Blood Glucose Levels and Lipid Profile in Diabetes Mellitus Patients Andika Aliviameita; Nevy Elensya; Puspitasari Puspitasari; Sri Mukhodim Faridah Hanum
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diabetes mellitus and its complications are still a major health problem in the world. Diabetes mellitus is closely related to the lipid profile, especially concerning the cardiovascular disease. A method that can be used to treat diabetes mellitus is hyperbaric oxygen therapy (HBOT). This study aims to observe changes in blood glucose levels and lipid profiles in patients with diabetes mellitus with hyperbaric oxygen therapy. The determination of blood glucose levels and lipid profiles is done by photometry. A total of 30 patients with diabetes mellitus examined for their blood glucose level and lipid profile before and after hyperbaric oxygen therapy. In the Paired T-test, there were significant decrease in blood glucose levels (p= 0,000), total cholesterol (p= 0,000), and triglycerides (p= 0,000) after hyperbaric oxygen therapy. The conclusion of this study shows there is a significant decrease in blood glucose level and lipid profile (total cholesterol, and triglycerides) after hyperbaric oxygen therapy.

Increased Mean Platelet Volume (MPV) in Patients with ST Elevation Myocardial Infarction (STEMI) Compared to Non-ST Elevation Myocardial Infarction (NSTEMI) Patients Tri Ratnaningsih; Istiqomah Istiqomah
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Platelets are known to have a role major in the pathogenesis of atherothrombosis. More giant and hyperreactive platelets accelerate the formation of an intracoronary thrombus. An increased MPV as an indicator of larger and more reactive platelets represents a risk factor for overall vascular mortality, including myocardial infarction. This study aimed to identify the increase of Mean Platelet Volume in Patients with Acute Coronary Syndromes, especially in ST-elevation myocardial infarction and Non-ST-elevation myocardial infarction. Thus this parameter can be used as consideration for diagnosis and treatment decisions. This research is an analytic observational with a cross-sectional method. The subjects were patients with STEMI and NSTEMI who were hospitalized in ICCU Dr.Sardjito Hospital Yogyakarta. Thirty-four subjects STEMI and NSTEMI patients at Dr. Sardjito Hospital, consisting of 28 males (82,35%) and six women (17,65%) with a mean age of 55,5 ± 10,3 years. The results showed that MPV in AMI was higher in patients with STEMI than NSTEMI, but this difference is not significant (p = 0,091). This study concludes that MPV to be higher in patients with STEMI compared to NSTEMI. MPV may be used as a marker of myocardial infarction in clinically appropriate situations.

The Place of Histochemical Stains for Differential Diagnosis in Duodenal Biopsies Ebru Altındal Susem; Mehtap Eroğlu; Elif Acar; İrfan Bayram
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The biopsy is the primary examination method in diagnosing duodenal pathologies and Gluten enteropathy that can cause symptoms such as gas, diarrhea, weight loss, anemia, osteoporosis. Allows monitoring of response to therapy. Histochemical studies are inevitable in specific findings such as reactive changes, giardia, subepithelial collagen thickening, fibrosis, and gastric metaplasia. We aimed to understand the contribution of histochemical applications to differential diagnosis and to show their superiority compared to hematoxylin-eosin sections in routine use by simultaneously making histomorphological evaluations on hematoxylin and eosin sections. This research is a retrospective study conducted in 2011, using the archives of Yüzüncü Yıl University, Faculty of Medicine, Department of Pathology, between 2001-2010. Ten normal, 50 Gluten enteropathy and 50 duodenitis samples; Along with histopathological examinations, we applied Masson Trichrome, Periodic acid-Schiff, Periodic acid-Schiff with diastasis, Periodic acid Schiff-Alcian Blue, High Iron Diamine-Alcian Blue. Chi-square, likelihood ratio tests, and SPSS (ver: 13) statistical package program used. We detected microscopic findings parallel to diagnostic criteria. While the number of goblet cells and intraepithelial neutrophils did not differ in the groups (P =0.176 and P=0.096), there was a significant variation in the intraepithelial lymphocyte count (P=0.010). The frequency of flattening and blunting of the villi, crypt hyperplasia, and Brunner gland hyperplasia were significant in gluten enteropathies (P=0.000). Conclusion We could not find any difference in specific pathologies associated with histochemical studies in our group, so hematoxylin-eosin sections are more valuable in the differential diagnosis.

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Ricko Dharmadi Utama

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Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA