Medical Laboratory Technology Journal
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
Articles
193 Documents
<![CDATA[Comparative study of diagnostic methods for determining the incidence of malaria parasite among children attending the State Specialist Hospital Ikare-Akoko, Ondo State, Nigeria ]]>
<![CDATA[Festus Abiose Olajubu]]>;
<![CDATA[Esther Olubunmi Ojo]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i1.195
<![CDATA[Malaria is an endemic disease in Sub-Saharan Africa, and it accounts for the death of more than 1million children annually. The objective of this study was to determine the incidence of malaria parasite among children between 2-15 years of age visiting State Specialist Hospital Ikare-Akoko, Ondo State, Nigeria, using Rapid diagnostic test kits and staining methods. The study does from July to December 2017. Two millimeters of venous blood samples were collected from each of the 300 enlisted children and stored in an anticoagulated specimen bottle. Thick and thin blood films prepared from the collected samples, stained and examined for the presence of malaria parasite use the Microscope. Three Rapid diagnostic test kits were also used. Two hundred and thirty-six (78.68%) of the 300 children investigated were found to have malaria parasite infection. Gender distribution showed that more male (45.67%) were infected than females (33.01%). Infections due to age distribution revealed that children within the ages of 2-5 years were more infected (39.3%) than children in other age range (6-10 years, 23.34% and 11-15 years, 17%). There was no statistical difference in the results obtained from the various techniques used (p]]>
<![CDATA[Acute Toxicity Test of Black Pomegranate Peel Extract (Granati Fructus Cortex) Against Larvae of Shrimp (Artemia salina Leach) ]]>
<![CDATA[Rofiatu Sholihah Achmad]]>;
<![CDATA[Lingga Aditya Aditya]]>;
<![CDATA[Nosa Ika Cahyariza]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.242
<![CDATA[Toxicity test on red pomegranate has done, while in black pomegranate is not widely known. Black pomegranate peel (Granati Fructus Cortex) has some chemical ingredients such as saponins, flavonoids, tannins, and alkaloids. Alkaloids, saponins, and flavonoids are thought to be toxic at certain levels. This study aimed to determine the effects of toxic black pomegranate peel extract (Granati Fructus Cortex) against larvae of shrimp (Artemia salina Leach) indicated LC50 values below 1000 µg / ml. This study is purely experimental by using Brine Shrimp Lethality Test (BSLT). The experiments divided into five groups, namely ethanol extract of black pomegranate peel (Granati Fructus Cortex) with a concentration of 100, 150, 200, 250 ppm, and negative control (seawater). Mortality data percentage of Artemia salina Leach analyzed by probit analysis. Results showed that the extract of black pomegranate peel (Granati Fructus Cortex) has a toxic effect on larvae of Artemia salina Leach with LC50 values of 114.090 µg/ml. From these results, it can conclude that black pomegranate peel extract has a potential acute toxic to larval shrimp (Artemia salina Leach) with Brine Shrimp Lethality Test (BSLT) method.]]>
<![CDATA[Serum Uric Acid Level in Unipolar and Bipolar Depression ]]>
<![CDATA[Gülçin Elboğa]]>;
<![CDATA[Muhammet Berkay Özyürek]]>;
<![CDATA[Zeynel Abidin Sayıner]]>;
<![CDATA[Şengül Kocamer Şahin]]>;
<![CDATA[Abdurrahman Altındağ]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i1.202
<![CDATA[The purinergic system plays a role in the regulation of mood, motor activity, cognitive function, sleep, and behavior. Purinergic mechanisms can also play a role in various neuropsychiatric diseases. The objective of this study is to assess whether uric acid levels in patients with unipolar and bipolar depression are different in comparison to healthy controls and to determine the clinical parameters that can be associated with the uric acid level. This retrospective study consisted of 31 patients with major depressive disorder and 31 patients with bipolar disorder depressive episode and 31 healthy control subjects. The mean serum uric acid levels were found as follows: major depression patient group 4.56 (± 1.53) mg/dL, bipolar depression patient group 5.38 (± 1.43) mg/dL and control group 4.86 (± 1.56) mg/dL. There was no significant difference between patients and the control groups in terms of serum uric acid levels (P=0.075). Serum uric acid levels do not differ significantly in bipolar and unipolar depression. Also, there was no difference between patients and control. Therefore, studying the uric acid metabolism in major depression and bipolar disorder depressive episode according to the symptom severity with larger sample groups is suggested.]]>
<![CDATA[Young and Old Sugar Apple (Annona squamosa Linn) Leaf Extracts As an Aedes aegypti Larva Insecticide ]]>
<![CDATA[Abdul Khair]]>;
<![CDATA[Noraida Noraida]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.231
<![CDATA[Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).]]>
<![CDATA[Investigation of the Pathogenesis and Treatment Efficiency of Bevacizumab-Induced Hypertension in the Rat Model ]]>
<![CDATA[Mehmet Ali Balci]]>;
<![CDATA[Musa Özgür Özyiğit]]>;
<![CDATA[Volkan İpek]]>;
<![CDATA[İlker Mustafa Kafa]]>;
<![CDATA[Ender Kurt]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i1.209
<![CDATA[Bevacizumab is known to reduce Vascular Endothelial Growth Factor (VEGF) to undetectable levels when used in conjunction with chemotherapy. Hypertension is a frequent adverse effect of bevacizumab, although its mechanism(s) remain unclear. In this study, our aim was to examine the pathogenesis of bevacizumab-induced hypertension and to investigate the treatment efficacy of valsartan. A total of 24 Wistar Albino female rats were included in the study. Rats were divided into three groups with 8 rats in each, as follows: The control group, bevacizumab group and bevacizumab + valsartan group. Blood pressure, blood urea nitrogen and serum creatinine levels were measured, urine samples were collected for 24 hours statistical analyses were performed using IBM SPSS 20 software pack. Nephrectomy specimens in bevacizumab and bevacizumab + valsartan groups exhibited varying degrees of renal injury. Although valsartan was able to reduce the bevacizumab-induced rise in blood pressure, it could not prevent the development of nephropathy. Conclusions these findings suggest that hypertension occurring secondary to bevacizumab treatment in rats may be associated with mechanisms involving renal injury.]]>
<![CDATA[Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte ]]>
<![CDATA[Puspitasari Puspitasari]]>;
<![CDATA[Evi Rinata]]>;
<![CDATA[Rohman Dijaya]]>;
<![CDATA[Siska Aprilia]]>;
<![CDATA[Dina Trikumalasari]]>;
<![CDATA[Livia Nur Azzah]]>;
<![CDATA[Qilmia Fanani]]>;
<![CDATA[Miftahul Mushlih]]>;
<![CDATA[Andika Aliviameita]]>;
<![CDATA[Dian Delta]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.229
<![CDATA[Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p]]>
<![CDATA[The Comparison of Widal Slide Examination Results between Tubex TF on Febrile Observation Patients Over 3 Days ]]>
<![CDATA[Nosa Ika Cahyariza]]>;
<![CDATA[Rofiatu Sholihah]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i1.212
<![CDATA[Typhoid fever is systematic bacterial disease usually occurs and has a high mortality rate each year, a disease transmitted from person to person due to contamination of feces, food, and water. The cause is bacterium Salmonella enterica serovar Typhi (S. Typhi) which is a natural host and reservoir for human. The limitations of the diagnostic test led to the increasing mortality rate due to typhoid fever. Besides ensuring infection in individuals, accurate serological tests are needed to ascertain the actual burden of the disease. Serological tests which are usually carried out in Puskesmas and hospital are Widal and Tubex Tf examinations. This study aims to determine whether there are differences in Widal and Tubex TF serological examinations in febrile patients over three days non-typhoid so patients can immediately find out whether they have typhoid fever or not. This study used a laboratory exploration method by examining 24 samples using Widal TYDAL and TUBEX® TF IDL Biotech. As many as 24 samples were examined by widal with antisera O, H, AH, and BH. Twenty-four of the same samples analyzed by TUBEX® TF. Results comparison of diagnostic from both methods will be compared using Mc Nemar test with significance = 0.05. Based on the examination which had done, it showed the difference in the results of Widal slide and lg M Anti Salmonella (Tubex Tf) in patients with febrile observation over three days. So, it can conclude that Tubex Tf examinations were better that widal slide examination because Tubex Tf uses Salmonella typhi anti-O9 antigen which can distinguish these organisms from >99% other Salmonella bacteria serotypes so that Tubex Tf examination is more specific.]]>
<![CDATA[Anti-hyperglycemic Effect of Torbangun (Coleus amboinicus Lour) Leaves Extract Through Liver and Muscle Glycogen Deposits in Streptozotocin-induced Hyperglycmic Sprague-Dawley Rats Model ]]>
<![CDATA[Meilla Dwi Andrestian]]>;
<![CDATA[Rizal Damanik]]>;
<![CDATA[Faisal Anwar]]>;
<![CDATA[Nancy Dewi Yuliana]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.227
<![CDATA[The association of liver and muscle glycogen deposits with serum insulin levels, β-cells pancreas, and fasting blood glucose (FBG) of streptozotocin (STZ)-induced hyperglycemic rats receiving Torbangun leaves extract (TE) investigated. The intervention performed on 25 8-week-old Sprague-Dawley rats divided into four groups. Seven rats separated as a normal group (N), and other rats injected with streptozotocin (STZ). Confirmation of hyperglycemic was characterized by fasting blood glucose >126 mg/dl. Treatment group which is NG (hyperglycemic rats); N (normal rats); H-IM (62.5 mg/kg BW metformin); and H-IT (620 mg/kg BW TE) for 14 days. This study revealed that TE significantly decreased FBG levels, increased insulin production, and the amount of liver glycogen deposits (a=0.01). However, the intervention did not significantly increase the amount of muscle glycogen deposits. TE administration improves β-cells, increases the liver and muscle glycogen deposits. TE was shown to have antihyperglycemic activity by improving the β-cell, increasing blood serum insulin levels, decreasing blood glucose levels, and increasing the liver glycogen deposits.]]>
<![CDATA[Prognostic Value of Neutrophil to Lymphocyte Ratio and Platelet to Lymphocyte Ratio in Lung Cancer ]]>
<![CDATA[Seda Beyhan Sagmen]]>;
<![CDATA[Coskun Dogan]]>;
<![CDATA[Sevda Comert]]>;
<![CDATA[Nesrin Kiral]]>;
<![CDATA[Ali Fidan]]>;
<![CDATA[Elif Torun Parmaksiz]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i1.211
<![CDATA[Numerous studies determined that the neutrophil/lymphocyte (NLR) and platelet/lymphocyte ratios (PLR) had prognostic value in several cancer types. This study aimed to evaluate the relationship between NLR and PLR values with the survival time of lung cancer patients. Patients diagnosed with lung cancer between January 2014 and December 2016, were retrospectively evaluated. Demographic characteristics, disease stages, laboratory parameters recorded, and the relationship of NLR and PLR values with the survival time and the disease stage evaluated. NLR and PLR were categorized into two groups. SPSS 17.0 software package was used for the statistical analysis. ROC analysis, Student T-test, Chi-square, and Mann-Whitney U test were used. Mean age of 62±8 years were included in the study. In the NSCLC group, the average NLR and PLR values were 4±3.35 and 194.6±144.4 respectively. Regarding the NSCLC group, the overall survival time was shorter in the subgroup with an NLR >3.43 (13.1 months) compared to the subgroup with an NLR ≤3.43 (24.3 months). The mean survival time was shorter in the group with a PLR > 136.9 compared to the group with a PLR ≤136.9 (15.9 and 24.6 months respectively). Subgroups consisting of survivors and non-survivors in the NSCLC group showed a statistically significant difference considering neutrophil and lymphocyte count, CRP, NLR, and PLR values (p]]>
<![CDATA[Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia ]]>
<![CDATA[Rubiati Hipni]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v5i2.247
<![CDATA[This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p]]>
