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INDONESIA
EUGENIA
Published by Universitas Sam Ratulangi
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Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The aims of this journal is to provide a venue for academicians, researchers and practitioners for publishing the original research articles or review articles. The scope of the articles published in this journal deal with a broad range of topics, including: Agronomi, Protection, Soil, Forestry, Agroecotecnology.
Arjuna Subject : -
Articles 198 Documents
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HUBUNGAN SPESIALISASI SEL DENGAN KANDUNGAN IAA PADA KULTUR SEL Catharanthus roseus DENGAN PENAMBAHAN TRIPTOFAN Pandiangan, Dingse; Tilaar, Wenny; Nainggolan, Nelson
EUGENIA Vol 18, No 2 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.2.2012.3952

Abstract

ABSTRACT The corelation cell specialization with IAA (indole acetic acid) content  of Catharanthus roseus cell culture by the addition of tryptophan has been done. This research is part of a strategy to increased the catharanthine content. One strategy used is the addition of tryptophan as a precursor treatment. IAA was other compound of the effect of the addition of tryptophan. Research was conducted in the laboratory using MS medium with tryptophan 50-250 mg/L. The cell morphology observations made by Halogen Nikon microscope and documented with a digital camera Nikon DXM 1200F. The results showed that cell morphology is essentially the same for each treatment. The differences seen is the presence of more the long cells in the treatment of tryptophan. The longest ratio is the treatment of 150 mg/L tryptophan at day 14 of culture. Changes in cell morphology relationship more long (run specialization) has something to do with the IAA content after treatment tryptophan. The highest IAA content equal 214,79±0,90  µg/g dw  was occurred at day 14th after being treated tryptophan 150 mg/L with 75% long cells or cells specialization. The relationship between the content of IAA specialized cells showed a positive correlation of 0.80 or 80% of each other. Key words: Cell specialization, IAA content, catharanthine, tryptophan, Catharanthus roseus ABSTRAK   Hubungan antara spesialisasi sel dengan kandungan IAA (asam indol asetat) kultur sel Catharanthus roseus dengan perlakuan triptofan telah dilakukan. Penelitian ini merupakan bagian dari strategi peningkatan kandungan katarantin. Salah satu strategi yang digunakan adalah penambahan perlakuan triptofan sebagai prekursor. IAA merupakan hasil sampingan sebagai pengaruh penambahan triptofan. Penelitian ini dilakukan di laboratorium dengan menggunakan media MS dengan triptofan 50-250 mg/L. Pengamatan spesialisasi sel dilakukan dengan mikroskop Nikon Halogen  dan didokumentasikan dengan kamera digital Nikon DXM 1200F. Bentuk sel pada dasarnya sama untuk setiap perlakuan. Perbedaan yang terlihat nyata adalah adanya sel-sel panjang lebih banyak pada perlakukan triptofan. Perlakuan yang paling panjang rationya adalah pada perlakuan 150 mg/L triptofan pada hari ke-14 kultur. Hubungan perubahan bentuk sel yang lebih banyak panjang (mengalami spesialisasi) ada hubungannya dengan kandungan IAA dan katarantin yang meningkat selelah perlakuan triptofan. Kandungan IAA paling tinggi yaitu sebesar 214,79±0,90  µg/g bk  terjadi pada hari ke 14 selelah diberi perlakuan triptofan 150 mg/L dengan persentasi sel panjang atau sel mengalami spesialisasi 75%. Hubungan antara spesialisasi sel dengan kandungan IAA menunjukkan hubungan yang positif sebesar 0,80 atau 80% saling menunjang. Kata kunci: Spesialisasi sel, IAA, katarantin, triptofan, Catharanthus roseus
PENGGUNAAN INSEKTISIDA BOTANIS UNTUK MENGENDALIKAN HAMA PADA TANAMAN TOMAT Rante, C. S.; Sembel, D. T.; Meray, E. R.M.; Ratulangi, M. M.; Dien, M. F.; Kandowangko, D. S.
EUGENIA Vol 19, No 2 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.19.2.2013.7374

Abstract

ABSTRACT   The experiment was conducted in the village of Touure, Tompaso District, Minahasa regency since April to August 2013. Designed studies using randomized block design with 3 treatments, namely fruit extract Bitung (treatment A), tuba root extract (treatment B) and water (control treatment), each treatment was repeated 3 times. Results showed that that the extract of Barringtonia asiatica and Derris elliptica has a botanical insecticide to control the properties of N. tenuis. Applications B. asiatica extract and D. elliptica were not significantly different, but both are significantly different from controls. In addition to N. tenuis found several Plant Pest Organisms (OPT) that attack tomato plants including pest Liriomyza sp., leafhoppers tomatoes., black stem base diseases, leaf spot diseases and viral diseases. Key words : Botanic Insecticide, Tomato pest control ABSTRAK   Penelitian dilaksanakan di Desa Toure, Kecamatan Tompaso, Kabupaten Minahasa sejak bulan April sampai dengan Agustus 2013.  Penelitian dirancang menggunakan Rancangan Acak Kelompok dengan 3 perlakuan yaitu ekstrak buah bitung (perlakuan A), ekstrak akar tuba (perlakuan B) dan air (perlakuan kontrol) yang masing-masing perlakuan diulang sebanyak 3 kali. Hasil penelitian menunjukkan bahwa bahwa ekstrak Barringtonia asiatica dan Derris elliptica memiliki sifat insektisida botanis untuk mengendalikan N. tenuis. Aplikasi ekstrak B. asiatica dan D. elliptica tidak berbeda nyata, namun keduanya berbeda nyata dengan kontrol.  Selain N. tenuis ditemukan juga beberapa Organisme Pengganggu Tanaman (OPT) yang menyerang tanaman tomat diantaranya hama Liriomyza sp., wereng tomat., penyakit pangkal batang hitam, penyakit bercak daun dan penyakit virus. Kata kunci : Insektisida botanis, pengendalian hama tanaman tomat
IDENTIFIKASI PENANDA ISOZIM PER-7, PER-8, DAN RAPD OB17375 PADA KELAPA GENJAH SALAK (GSK) DAN BEBERAPA HASIL SILANGANNYA DENGAN KELAPA DALAM Runtunuwu, Semuel D.; Lengkong, Eddy F.
EUGENIA Vol 11, No 1 (2005)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.11.1.2005.11882

Abstract

ABSTRACT Runtunuwu, S.D. and E.F. Lengkong. 2005. Identification of Isozyme Markers (PER-7, PER-8, and RAPD OBI7375) of Dwarf Salak Coconut (GSK) and its Tall Hibrids. Eugenia 11 (1): 8-17. The objective of this research was to identify PER-7 and PER-8 isozymes, and OB17 375 RAPD as molecular markers for resistance to NF Phytophthora disease in Genjah Salak (GSK) dwarf coconut and its several hybrids with Tall coconut at The Research Institute for Coconut and Palmae (RICP) Manado. Coconut resistance to NF Phytophthora disease was determined based on disease lesion size of inoculated coconut fruits at 7 days after inoculation. PER-7 and PER-8 isozymes marker were identified using horizontal starch gel electrophoresis, and OB17375 RAPD marker was identified based on polymerase chain reaction (PCR) using kit B number 17 primer (Operon Technologies, California). PER-7 isozyme marker could be used to select GSK x DTA (Dalam Tenga) and GSK x RLT (Rennell Tall) hybrid coconuts that resistant to NF Phytophthora disease. PER-7 isozyme without OB17375 markers (PER-7/-) could be used to select resistant GSK x WAT (West African Tall) hybrid coconut to the disease. Keywords: Isozymes, RAPD, Molecular marker, Phytophthora disease, coconut
PENGGUNAAN PATI SAGU MODIFIKASI FOSFAT TERHADAP SIFAT ORGANOLEPTIK SOSIS IKAN PATIN (Pangasius hypophtalmus) Koapaha, Teltje; Langi, Teneke; Lalujan, Lana E.
EUGENIA Vol 17, No 1 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.1.2011.103

Abstract

Beef and chicken sausages have been widely popular in all classes of the society, howeverfish sausage has not being recognized and not available in the market yet. In the production offish sausage, the ratio between fish flesh and tapioca as well as other igredients such ascooking salt, sugar, skim-milk , ice and flavoring agent played important role on the quality offish sausages. This study aimed to find out the effect ratio of composite sago starch on theorganoleptic quality of fish sausage. The randomized design were used in this experiment.The experiment was repilicated three times and the parameters observed organoleptic weretaste, texture, colour, flavor of properties fish sausage.The modification treatment were theusing 0.25% STPP. Result showed that the composite sago starch of 50:50 and10% of it usedin fish sausage production gave the most preferable organoleptic properties with the texture0.04 mm/g.sec, taste, colour, flavor of properties fish sausage. ABSTRAKSosis dari daging ayam dan sapi sudah dikenal masyarakat luas , namun sosis dari dagingikan belum dikenal bahkan belum dijual di pasaran. Pada proses pembuatan sosis daridaging ikan , rasio daging ikan dan pati serta bahan tambahan lain seperti gula garam , sususkim, es dan bumbu penyedap berperan penting dalam menentukan kwalitas sosis ikan . Halini erat hubungannya dengan pembentukan mtriks gel protein, pati lemak dalam bentuk systememulsi sosis yang baik. Tujuan dari penelitian yaitu mempelajari pengaruh rasio pati sagukomposit terhadap sifat organoleptik. Menggunakan Rancangan Acak Lengkap faktor tunggalyaitu pati sagu modifikasi fosfat konsetrasi 0,25% STPP dalam pati sagu komposit 50:50 danRasio pati sagu komposit terhadap daging ikan 10%; 20%; 30%, diulang 3 kali. Pengamatanorganoleptik meliputi :rasa, tekstur, warna, bau sosis ikan. Hasil penelitian perlakuan terbaikdiperoleh pada pati sagu komposit yaitu pati sagu alami diambah dengan pati sagu modifikasi50:50, rasio 10% terhadap daging ikan dengan hasil uji organoleptik terhadap rasa, tekstur,warna, baus paling disukai panelis, nilai tekstur dengan alat penetrometer yaitu 0,04 mm/g.det.
PEMUPUKAN NPK MAJEMUK PADA BIBIT JABON MERAH (Anthocephalus macrophyllus (Roxb.) Havil) Luhulima, F. S.; Lasut, M. T.; Kainde, R. P.; Thomas, A.
EUGENIA Vol 23, No 3 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.23.3.2017.18967

Abstract

ABSTRACT Various doses of NPK fertilization  could increase plant growth. This research studied  the effect of NPK compound fertilizer with some kind of dose on the growth of seedlings of red jabon. This research was conducted at the Laboratory of Silviculture Faculty of Agriculture, University of Sam  Rarulangi which lasted from May until June 2012, The objectives of this study were to determine the effect of compound NPK fertilizers on the growth of seedlings of red jabon. The research used experimental design of Randomized Block Design (RBD), Each treatment was replicated 5 times and each replication consisted of 5 red jabon seedlings. The treatments being given were: (1) without fertilizer (control), (2) 1 g of NPK/ plant, (3) 2 g of NPK/plant, (4)  3 g of NPK/plant and (5) 4 g of NPK/plant.  The results of this research showed that compound fertilizer NPK influence to high growth, diameter,  number of leaves, root volume and dry weight of seedlings red jabon canopy. NPK fertilizer with a dose of 3 g/plant  (D) can increase height growth,  diameter and number of leaves. While the parameters of the root volume and dry weight of the canopy, NPK treatment media with 4 g/plant (E) gives better results.Keywords : NPK fertilization, growth seedling, red jabon ABSTRAK Pemupukan NPK dengan berbagai dosis dapat meningkatkan pertumbuhan tanaman. Dalam penelitian ini dipelajari pengaruh pemupukan majemuk NPK dengan beberapa macam dosis terhadap pertumbuhan bibit jabon merah. Penelitian ini dilaksanakan di Laboratorium Silvikultur Fakultas Pertanian Universitas Sam Rarulangi yang berlangsung mulai Mei s/d Juni 2012. Tujuan dari penelitian ini ialah untuk mengetahui pengaruh pemupukan majemuk NPK terhadap pertumbuhan bibit jabon merah. Penelitian ini menggunakan metode eksperimen Rancangan Acak Kelompok (RAK), setiap perlakuan diulang sebanyak 5 kali dan setiap ulangan terdiri atas 6 bibit jabon. Perlakuan yang diberikan adalah : (1) tanpa pupuk (kontrol), (2) pupuk NPK 1 gram/tanaman, (3) pupuk NPK 2 gram/tanaman, (4) pupuk NPK 3 gram/tanaman dan (5) pupuk NPK 4 gram/tanaman. Hasil penelitian menunjukkan bahwa terdapat pengaruh dosis pupuk majemuk NPK terhadap pertumbuhan tinggi, diameter, jumlah daun, volume akar dan berat kering tajuk bibit jabon merah. Pemupukan NPK dengan dosis 3 gram/tanaman (D)  dapat meningkatkan pertumbuhan tinggi, diameter dan jumlah daun. Sedangkan pada parameter volume akar dan berat kering tajuk, media dengan perlakuan NPK 4 gram/tanaman (E) memberikan hasil yang lebih baik.Kata kunci :  pemupukan NPK, pertumbuhan bibit, jabon merah
PENAMBAHAN GIBERALIN (GA3) DAN SUHU DINGIN TERHADAP MASA SIMPAN BUNGA GLADIOL DALAM KEMASAN PLASTIK Longdong, Ireine A.; Lengkey, Lady; Kairupan, Stella
EUGENIA Vol 17, No 3 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.3.2011.3549

Abstract

ABSTRACT   This study aimed to obtain the concentration and duration of immersion Giberalin type GA 3 the most effective way to extend the shelf life of gladiolus flowers, and to get the type of packaging and the right temperature in prolonging the shelf life of gladiolus flowers to prolong shelf life. Descriptive method was used in two experiments. The first experiment was  GA3 concentration and immersion time on the shelf life of gladiolus flowers. The second experiment was  the type of packaging and cold temperatures on the shelf life of gladiolus flowers.  The result of first experiment showed that  gladiolus flowers  soaked at a concentration of 250 ppm GA3 and 1 hour immersion time gives had the lowest value. CO2 production was ranged from mlCO2/kg.hours 18:43  to 33.30 mlCO2/kg.hours. Severe shrinkage rates in day two ,four and six were    27.92%, 36.31% and 40.27% respectively. The degree of gladiolus flowers bloom in the day two was  42.85%  and degree of  on day four was 33.63%. The results of second experiment showed that gladiolus flowers packed in polypropylene plastic storage temperature of 50C for 12 days resulted  the lowest value. CO2 production were ranged from 8.68 mlCO2/kg.hours - 15:10 mlCO2/kg.hours. The severe shrinkage in first  day was  1 27.29%. The degree of blooming in the 8 th day was  9.63%. Degree of wilting during 12 days storage was  0%. Keywords: Flowers gladiolus, Giberalin, cold temperatures, shelf life, plastic packaging
HASIL KACANG TANAH (Arachys hypogaea L.) PADA BEBERAPA JENIS PUPUK ORGANIK Sondakh, Tommy D.; Joroh, Djuhardi N.; Tulungen, A. G.; Sumampow, D. M.F.; Kapugu, Lita B.; Mamarimbing, Rinny
EUGENIA Vol 18, No 1 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.1.2012.4150

Abstract

ABSTRACT   This study aimed to find out the effects of several types of organic fertilizers on peanut’s yield. This study was conducted in the greenhouse environment using a randomized block design with of four treatments of organic fertilizer. Each treatment was repeated five times. Treatment A = without fertilizer; B = Liquid Organic Fertilizer Super Aci 4 cc / liter of water; C = Granular Organic matter 1000 kg / ha; and D = Humagrow 500 grams / ha. Observed variables included  1) plant dry weight, 2) numbers of pods , 3) numbers of empty pods, and 4) weight of pods. The results showed that some types of organic fertilizers influenced  the yield of peanuts. Organic fertilizers are super Aci 4 cc / liter of water and 20 tons of granular NPK / ha gave the highest yield. Key words:  Peanuts, super leveling compound organic fertilizer, granules organic matter   ABSTRAK Hasil penelitian kacang tanah (Arachys hypogaea L.) Pada Beberapa Jenis Pupuk Organik bertujuan untuk mempelajari pengaruh beberapa jenis pupuk organik pada tanaman kacang tanah. Penelitian menggunakan Rancangan Acak Kelompok (RAK) yang terdiri dari empat perlakuan pupuk organik. Tiap perlakuan diulang lima kali. Perlakuan tersebut adalah A = Tanpa pemupukan, B = Pupuk Organik Cair Super Aci 4 cc / liter air, C = pupuk Organik granular 1000 kg/ha, dan D = Humagrow 500 gram/ ha. Variabel yang diamati adalah 1) berat kering tanaman, 2) Jumlah polong berisi, 3) Jumlah polong hampa, dan 3) berat polong per tanaman. Hasil penelitian menunjukkan bahwa beberapa jenis pupuk organik mempengaruhi hasil kacang tanah. Pupuk organik Super Aci 4 cc / liter air dan 1000 kg pupuk organik granular / ha memberikan hasil kacang tanah tertinggi. Kata kunci: Tanaman kacang tanah, pupuk organik super aci, pupuk organik granul, dan humagrow
KERAGAMAN BUAH PALA (Myristica fragrans Houtt) DI KABUPATEN KEPULAUAN SANGIHE DAN KABUPATEN SITARO ., Robert; Runtunuwu, S. D.; Rogi, J. E.X.; Pamandungan, Yefta
EUGENIA Vol 21, No 3 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.21.3.2015.9721

Abstract

ABSTRACT   This study aims to determine the nutmeg crop diversity is based on the character of the fruit, seeds, nutmeg mace in Sangihe Island Regency (Island of Sangihe) and District SITARO (Siau Island). The method used is the method of direct observation at the farmer's planting area nutmeg. Location research based on secondary data, namely the districts and villages producer of nutmeg, then determined the three observation points in each study site. Each observation point is determined 10 samples to be observed fruit trees, and every tree observed 20 fruit ready for harvest. Samples of plants is determined by: 1) Age of plants, which is about 15-30 years, and 2) The state of the plant, which is being fruitful and healthy tree. Observations carried out on: 1) The shape of fruit, 2) Color rind 3) Thick flesh, 4) Weight pieces, 5) The length of the fruit, 6) diameter pieces, 7) The color of the fruit flesh, 8) Weight seed fresh, 9) Lengh seed, 10) diameter seed, 11) weight  mace fresh, 12) Color mace. The results showed: 1) The shape of fruit and nutmeg seeds in Sangihe Islands Regency is more diverse than in the District Sitaro. 2) The highest weight of fresh nutmeg obtained from the location Talawid weighing 57.95 g fresh weight and r lowest nutmeg obtained from the location Karatung I is weighing 35.91 g fresh weight nutmeg while the highest was obtained from the location that is weighing 9.45 g Talawid and the lowest seed fresh weight was obtained from the location Karatung I are weighing 6.75g. 3) The same colors are red blood mace. Keywords: nutmeg plant, diversity of fruit, Sangihe, SITARO
ISOLASI DAN KARAKTERISASI BAKTERI INDIGENOUS (Bacillus Cereus FRANK.) SEBAGAI AGENSIA PENGENDALI HAYATI HAMA KUBIS Salaki, Christina L.
EUGENIA Vol 17, No 1 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.1.2011.94

Abstract

B. cereus strains were isolated from three district of North Sulawesi. The B. cereus strains are potentialindigenous bacteria for biological control agent against P. xylostella. The B. cereus strains were isolatedusing Ohba and Aizawa methods. Two hundred and two isolates putatively were isolated and identifiedas member of species B. cereus. Subseguently, representing isolates were selected to be screened forthe ability to kill P. xylostella. The screening test was conducted by using leaf depped methods with theconcentration of 1,22x108 spore/ml. The potential to kill test insect was assessed by mortality value. Theresult indicated that 10 isolates out of 15 of could produce mortality of insect from 50 to 100% afterexposure of 96 hours. It was confirmed that based on symptoms produced as well as bacterialcharacteristics reisolated from cadaver of test insect, the death of the test insects was certainly causedby application of bacterial isolates. It could be concluded that indigenous bacterial isolates identified tobe member of B. cereusare potential for biological control agent against P. xylostella. ABSTRAKUntuk memperoleh bakteri yang berpotensi sebagai agensia pengendali hayati hama Plutela.xylostelladilakukan isolasi dan karakterisasi bakteri indigenous. (Bacillus cereus) yang diperoleh dari sampeltanah yang berasal dari tiga kabupaten di Sulawesi utara. Bakteri diisolasi dengan metode Ohba danaizawa. Dari 103 sampel tanah yang digunakan dapat diperoleh 202 isolat yang diidentifikasi sebagaianggota spesies B.cereus. Sebanyak 15 isolat dipilih untuk mewakili seluruh isolat dalam sereening ujikemampuan membunuh P.xylostella. Pengujian dilakukan dengan metode pencelupan daun (leafdipped method) pada konsentrasi 1,22x108 spora/ml. Kemampuan membunuh serangga uji dinyatakandengan nilai mortalitas setelah pendedahan selama 96 jam. Ternyata hanya 10 isolat yang dapatmenimbulkan mortalitas serangga uji dengan kisaran 50%-100%. Berdasarkan gejala penyakit yangmuncul, karakteristik bakteri hasil reisolasi dari cadaver serangga mati menunjukkan bahwa kematianserangga uji dipastikan disebabkan oleh isolat bakteri yang diberikan. Disimpulkan, bahwa isolat bakteriindigenous anggota spesies B.cereus yang diperoleh berpotensi untuk dikembangkan menjadi kandidatagensia pengendali hayati bagi serangga hama kubis P.xylostella.
REDESAIN RUANG TERBUKA HIJAU KAMPUS UNSRAT BERDASARKAN EVALUASI KENYAMANAN TERMAL DENGAN INDEKS DISC Saroinsong, Fabiola B.; Kalangi, J. I.; Babo, Prestin
EUGENIA Vol 23, No 2 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.23.2.2017.16778

Abstract

ABSTRACT Outdoor spaces are important to sustainable cities because they accommodate outdoor activities and contribute greatly to the quality of urban living.During the last decade, interest in the assessment of thermal comfort has increased because of climate changes and increased heat stress in cities. Greenspaces that provide a pleasurable thermal comfort experience for users effectively improve their quality of livability and productivity. However, current investigations lack a general framework for the use of thermal comfort assessment, such as in making master plan or designing better greenspace. This paper discusses greenspace of Sam Ratulangi University Campus relating to thermal comfort. It provides knowledge of thermal comfort investigation and landscape design review. The results of thermal comfort assessment, among 11 observed plots, only greenspace at FMIPA is categorized as comfortablespot. Greenspaces at FAPET and FEB are not really comfortable, those at BNI and FKM A and FKM B are not comfortable and those at FATEK, FAPERTA, FISIP, FH, FKM A are categorized as very uncomfortable spots. We suggest some options for redesigningof uncomfortable and very uncomfortable greenspace spots.Keywords: greenspace, outdoor, termal comfort

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