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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

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Published by Universitas Muhammadiyah Surabaya

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Core Subject : Health,

Health Professions

Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik

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Articles 202 Documents

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Keanekaragaman Genetik Nyamuk Vektor Filariasis Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) di Kota dan Kabupaten Pekalongan Dengan Metode PCR-RAPD Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Mosquito Culex quinquefasciatus is a vector of nematode worms, namely Wuchereria bancrofti which is the cause of filariasis in tropical and subtropical countries. Distributed of Cx. quinquefasciatus is widely in Indonesia with differences the geographical, resulting in the adaptation to the environment and may results in a high variation, both phenotypic (morphology) and genotypic (genetic) variation. This study aims was to determine the genetic diversity of mosquitoes Cx.quinquefasciatus as vector filariasis in Pekalongan City and Regent. Genetic characterization performed by PCR-RAPD using three primers, ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using UPGMA algorithm and Simple Matching Coefficient and presented as dendrogram. The results showed a high genetic diversity with the polymorphisms up to 100%. Keywords: Culex quinquefasciatus, vector, filariasis, PCR-RAPD

Bioaktif Belimbing Wuluh (Averhoa bilimbi (Linn.)) Terhadap Kadar Formalin Dalam Tahu Rinza Rahmawati Samsudin
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

This study aims to determine the influence of the juice of belimbing wuluh to the content of formalin in tofu and know the concentration of belimbing wuluh feeling is most effective in reducing the content of formalin in tofu. This study used a complete randomized design. The sample of this study was 30 tofu containing formalin which was divided into 6 groups. The weight of each sample is 15 grams.The data of the research were analyzed by using anova test with 95% confidence level, then Duncan test with 95% confidence level. From the result of this research can be concluded that there is influence to formalin content in tofu. The content of formaldehyde in the highest to the lowest yields was found in soaking the belimbing wuluh with the concentrations is the group without treatment (8269.40 mg / Kg), 0% (1434.20 mg / Kg), 100% (762.00mg / Kg), 25% (573.80 mg / Kg), 75% (442.20 mg / kg), and 50% (162.00 mg / kg). The highest decrease in formaldehyde content in tofu by 98.40% occurred in the group K3 with the most effective 50% concentration to decrease the formalin content in tofu. Keyword : belimbing wuluh, tofu, formalin

Gambaran Anti-Plasmodium sp. Pada Anggota Tentara Nasional Indonesia Angkatan Darat Di Kota Cimahi Patricia Gita Naully; Gina Khairinisa; Dewi Ratih Saputri
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Malaria is a disease caused by Plasmodium sp. The presence of such parasites in body can be characterized by the presence of anti-Plasmodium sp. antibodies. Malaria cases are still prevalent in Indonesia and generally occur in people who travel to endemic areas. One of the jobs that obliges its members to serve to various regions, including endemic areas is the Indonesian Army (TNI AD). Many Army personnel residing in Cimahi City. The purpose of this study is to determine the overview of malaria in members of the Army in Cimahi City. This study used a sample of 30 TNI AD who are residing in Cimahi City and have been assigned to various regions in Indonesia. Venipucture is only performed on respondents who have signed informed consent and filled out the interview form. Malaria screening is performed using immunochromatography that can detect anti-P. vivax and anti-P. falciparum antibodies, with a high degree of sensitivity and specificity. Of the 30 people, there were 4 people (13.3%) who had been infected with malaria and 2 (6.7%) of whom had experienced mixed malaria. All respondents who tested positive for malaria claimed to have been assigned to malaria endemic areas such as Papua and Maluku for several years.

Uji Efektivitas Formula Pedikulosida Berbahan Aktif Minyak Atsiri Terhadap Mortalitas Kutu Kepala (Pediculus humanus capitis De Geer) Secara In Vitro Tanendri Arrizqiyani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Pediculosis is a disease caused by Pediculus humanus capitis de Geer or often called head lice which is an obligate ectoparasite in the human head. The prevalence of pediculosis is quite high and there are reports of resistance and negative side effects regarding the use of synthetic pediculosides trigger research and development of vegetable pediculosides. This study aims to examine the effectiveness of the formula of pediculoside with active ingredients of essential oils on head lice mortality in vitro. The study was conducted by testing 3 formulas with variations in the composition of essential oils made from cassava oil, fennel oil, lemon oil, water and vegetable glycerine. Comparison of the ingredients in formula A is 10%, 10%, 5%, 50% and 20%; formula B is 5%, 15%, 5%, 50% and 20%; while formula C is 15%, 5%, 5%, 50% and 20%. The test method is an experiment with a completely randomized design. The test is done by placing 10 adult head lice on filter paper which has been saturated with a formula made in a petri dish. Mortality of head lice is observed based on time. Test results data will be compared with negative controls and positive controls in the form of testing using permethrin-active pediculoside drugs. The results showed that the pediculoside formula A, B and C had a high mortality rate which caused 100% of the deaths of head lice tested in less than 5 minutes. Statistically there is a significance to the results of testing formulas A, B, and C when compared to controls. Based on the results of multiple statistical statistics, it was found that formula C was the most effective as a pediculoside formula

Efek Pemberian Gentamisin Secara Oral Terhadap Kadar Asam Urat, Ureum, dan Kreatinin Tikus Wistar Syahrul Ardiansyah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

ABSTRACTGentamicin is an aminoglycoside class of antibiotics which at high doses can cause damage to the kidneys. This research was to determine the levels of BUN, creatinine and amino acids after gentamicin induction. This research is experimental with gentamicin test material given to male rats as much as 0.3 ml/day for 7 and 10 days. Blood serum levels were then measured for BUN, creatinine and amino acids using a photometer microlab 300. The results showed that after doing treatment for 7 days the average value of BUN levels increase from 21.7 to 36.3 mg/dl, creatinine increase from 0, 88 to 1.9 mg/dl, uric acid increase from 1.54 to 4.29 mg/dl. It was concluded that giving of gentamicin as much as 0.3 ml/day for 7 and 10 days increased BUN levels, creatinine and uric acid exceeded the normal limit. Keywords : Gentamicin, urea, creatinine, uric acid, rat.

Analisa Asam Salisilat Pada Perlengkapan Persedian Bermerk Bayi Di Pasar Blauran Surabaya Nastiti Kartikorini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Salicylic acid is a substance that is often added to skin care products for acne. In the regulation of the National Agency of Drug and Food Control No. Hk.03.1.23.08.11.07517 in 2011 concerning the technical requirements of cosmetics, salicylic acid levels are limited to 3% for rinse production and 2% for other production and are not used for children under 3 years. Using at high doses can cause the baby to bleed, mute and deaf. This is because the structure of the baby's skin is still thin, so it becomes susceptible to irritation and infection. The title of this research is Analysis of Salicylic Acid in the mains supplies barnded baby in the market blauran in Surabaya Region. The purpose identified salicylic acid content with qualitative tests on baby cosmetics circulating in Surabaya. The formulation of this problem is the presence or absence of Salicylic Acid in baby cosmetics. The type of research used descriptive, there were 26 baby cosmetics products in 4 types of products in the form of powder, soap, shampoo, and lotion which were conducted in the Laboratory of Muhammadiyah University of Surabaya in June 2018. Based on the results that found from 26 samples of baby cosmetics in the form of powder as much as 100% of soap, shampoo and lotions sold in Surabaya were not found for Salicylic Acid. So that all samples met the requirements based on the regulation of the National Agency of Drug and Food Control No.Hk.03.1.23.08.11.07517 in 2011 concerning the technical requirements of cosmetics materials, salicylic acid levels were limited to 3% not used for children under 3 years. Keywords: Baby supplies, Salicylic Acid

Gambaran Bakteri Kontaminan pada Sikat Gigi Perdina Nursidika; Patricia Gita Naully; Linda Ayu Lestari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 1 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Abstract: DESCRIPTION OF BACTERIAL CONTAMINTANT IN TOOTHBRUSH. Brushing activity is an effort to keep oral hygiene from pathogenic microorganisms that can cause infection. Oral infection has been an increasingly common risk factor for systemic disease. Oral infections, especially periodontitis, can affect travel and pathogenesis of a number of systemic diseases, such as cardiovascular disease, bacterial pneumonia, diabetes mellitus, and low birth weight. Keeping your teeth and mouth clean is not enough just brushing your teeth, but replace the toothbrush for a maximum of three months. This study aims to identify the contaminant bacteria found on toothbrushes that have been used for more than 3 months. The method of identification by planting the isolation result of 20 toothbrushes has been used for more than three months on selective media ie blood agar (AD), McConkey Agar (MCA), Eosin Methylen Blue (EMB), Manitol Salt Agar (MSA) . The results showed that all samples of toothbrush were contaminated with Streptococcus and Staphylococcus, 16 samples were Klebsiella (80%), four samples (20%) were Proteus, and one sample (5%) was contaminated with Escherichia. All of the toothbrush samples showed to have been contaminated by bacteria. Bacterial contamination of the toothbrush can be caused by the storage of a toothbrush that is too close to the toilet and toilet, possibly exposed through aerosols containing intestinal bacteria. This toothbrush contamination plays an important role in both oral and systemic diseases, including septicemia and gastrointestinal, respiratory cardiovascularmy, and kidney problems. Abstrak: GAMBARAN BAKTERI KONTAMINAN PADA SIKAT GIGI. Kegiatan menyikat gigi merupakan upaya untuk menjaga kebersihan gigi dan mulut dari mikroorganisme pathogen yang dapat menyebabkan infeksi. Infeksi mulut telah menjadi faktor risiko yang semakin umum untuk penyakit sistemik. Infeksi mulut, terutama periodontitis, dapat mempengaruhi patogenesis dari sejumlah penyakit sistemik, seperti penyakit kardiovaskular, pneumonia bakteri, diabetes mellitus, dan berat badan lahir rendah. Menjaga kebersihan gigi dan mulut tidak cukup hanya menyikat gigi saja, melainkan mengganti sikat gigi maksimal tiga bulan sekali. Penelitian ini bertujuan untuk mengidentifikasi bakteri kontaminan yang terdapat pada pada sikat gigi yang telah digunakan selama lebih dari 3 bulan. Metode identifikasi dengan melakukan penanaman hasil isolasi 20 sikat gigi yang telah digunakan selama lebih dari tiga bulan pada media selektif yaitu agar darah (AD), McConkey Agar (MCA), Eosin Methylen Blue (EMB), Manitol Salt Agar (MSA) dengan metode gores. Hasil penelitian menunjukkan semua sampel sikat gigi terkontaminasi Streptococcus dan Staphylococcus, 16 sampel terdapat Klebsiella (80%), pada empat sampel (20%) terdapat Proteus, dan satu sampel (5%) terkontaminasi Escherichia. Semua sampel sikat gigi menunjukkan telah terkontaminasi oleh bakteri. Kontaminasi bakteri pada sikat gigi ini dapat disebabkan oleh penyimpanan sikat gigi yang terlalu dekat dengan jamban, sehingga kemungkinan terpapar melalui aerosol yang mengandung bakteri usus. Kontaminasi sikat gigi ini memegang peranan penting pada penyakit oral maupun sistemik, termasuk septikemia dan gastrointestinal, kardiovaskularm pernapasan, dan masalah ginjal.

Efektivitas Vitamin C Pada Daun Kelor Terhadap Bilangan Peroksida Dari Minyak Jelantah Nastiti Kartikorini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Used cooking oil is a cooking oil that has been used several times. Used cooking oil undergoes an oxidation reaction to produce high peroxide compounds. Phenolic compounds are primary antioxidants contained in Moringa leaves which slow the oxidation down in used cooking oil so that it can be stored longer. Formulation of the problem in this study was whether there was an inhibitory test on Moringa oleifera Leaves powder on the peroxide value in used cooking oil. The purpose of this study was to determine the inhibition of Moringa leaves powder to peroxide value on used cooking oil. This type of research was an experimental i.e. to determine whether there was the inhibition of Moringa leaves powder to the peroxide value in used cooking oil. The sample in this study was 5 samples from 6 fried traders, and repeated 5 times so that the total sample was 25 samples. Peroxide levels could be decreased due to the antioxidant content found in Moringa leaves. Antioxidants found in Moringa leaves are phenolic components. Phenolic components can inhibit free radicals and inhibit peroxide value to decrease. Moringa was suitable for areas where strong and long winds, drought occur simultaneously, and cause serious soil erosion. Nutrient content in deciduous leaves could fertilize and improve marginal soil quality (Dudi, 2015). Therefore, Moringa leaves not only have high antioxidants but also could be found easily in Surabaya. From the results of peroxide levels test, it was known that the average peroxide value in used cooking oil without Moringa leaves was equal to 23,9957 mEq and with Moringa leaves by different time variation i.e. 1 day (14,3182 mEq), 2 days (10,7987 mEq), 3 days (6,1593 mEq), 4 days (3,0397 mEq). Statistical analysis with Anova test showed the inhibition of Moringa leaves powder to decrease in peroxide value on used cooking oil with a value of p (sig) = 0,000 which was less than 0.05. Based on the results of the Tukey HSD test, the most effective soaking of Moringa leaves powder for 4 days was to reduce peroxide levels in used cooking oil by giving 5gr Moringa leaf powder with 100ml used cooking oil. Keywords : Levels of Peroxide Value, Moringa Leaves, Used Cooking Oil

HUBUNGAN FREKUENSI MENGURAS TERHADAP PERTUMBUHAN JAMUR PADA AIR BAK TOILET TEMPAT WISATA DI WILAYAH KOTA KEDIRI Durroh Humairoh; Endrik Asmarani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Tempat wisata merupakan suatu tempat menarik yang dituju bersama rekan, teman, ataupun keluarga untuk berlibur. Setiap tempat wisata selalu dilengkapi toilet yang bersih demi kenyamanan pengunjung. Pada tempat wisata yang menyediakan toilet umum harus selalu dibersihkan dengan teratur supaya tidak terinfeksi oleh mikroorganisme yang dapat membahayakan kesehatan. Pada penelitian ini, dilakukan penelitian pada beberapa toilet tempat wisata di wilayah kota Kediri. Metode yang dilakukan pada penelitian ini dengan centrifugasi sebanyak 15 tempat wisata di kota Kediri. Hasil penelitian dengan uji korelasi spearman’s rho menunjukkan terdapat hubungan frekuensi menguras dengan jumlah jamur yang tumbuh pada air bak toilet dan hasil tersebut menunjukkan bahwa semakin lama masa frekuensi menguras bak toilet, maka semakin tinggi pertumbuhan jamur yang muncul di air bak toilet tersebut. Kata kunci: frekuensi menguras, pertumbuhan kapang, tempat wisata

Uji Daya Serap Diapers Anti Ruam (Diapers Rash) Modifikasi Sabut Kelapa (Cocosnucifera) Siti Mardiyah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

The World Health Organization (WHO) reported in 2012 that the prevalence of skin irritation (diaper rash) in infants was quite high at 25% due to the use of diapers. One of the causes of diaper rash is caused by low diaper absorption. Increased absorption of diaper can be done by increasing the absorbent ability of diapers to absorb baby urine. The natural absorbent that can be modified in baby diapers is coconut fiber. Cellulose content in coconut fiber has the ability to absorb liquid high enough. This potential can be applied to absorb urine and sweat so that it can reduce moisture in baby diapers. Diapers that have high absorption can prevent the occurrence of rashes in infants. This study aims to examine differences in absorbency of diapers. Without coconut fiber and coconut husk modification diapers. This type of research is experimental. The population and sample of the study were baby diapers with a total sample of 32 which were divided into 2 groups namely baby diapers without coconut fiber and with modification of coconut fiber. Testing for diaper absorption is carried out in a laboratory manner by measuring the volume and time of absorption of fluids given to diapers to the saturation limit (leak). The data analysis technique uses the independent t-test. Based on the results of the independent t-test showed that there were significant differences in the amount of volume and time of absorption of fluids given to bunch diapers and with coconut fiber. Thus it can be concluded that there is a significant influence on the addition of coconut fiber to the absorption of fluid in baby diapers. Keywords: Diapers, Rash, Coconut Fiber.

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