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THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

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Published by Universitas Muhammadiyah Surabaya

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Core Subject : Health,

Health Professions

Merupakan media publikasi ilmiah yang memuat berbagai artikel ilmiah hasil tinjauan pustaka, dan hasil penelitian dalam bidang ilmu Patologi Klinik, Hematologi, Mikrobiologi, Parasitologi, Imunologi, Sitohistoteknologi, Kimia Kesehatan dan Ilmu Teknologi Laboratorium Medik

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Articles 202 Documents

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Uji Toksisitas Akut Kulit Kentang (Solanum tuberosum L.) Terhadap Larva Artemia salina Leach Dengan Metode BSLT (Brine Shrimp Lethality Test) Baterun Kunsah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Tanggal Submit:28 Januari 2019 Tanggal Review:30 April 2019 Tanggal Publish Online:17 Mei 2019 Potatoes are one of the vegetables that contain substances that are important for the formation of body tissues, such as protein and fat. Potatoes also form red blood cells or hemoglobin (Ca, P and Fe). In Indonesia tend to process potatoes using only meat. The skin is removed, even though the nutritional content of the skin is five times greater than the meat. Potato peels that supply querestin, antioxidants and flavonoids that act as free radical acceptors. (Free radicals are reactive molecules that cause damage to the body that can lead to diseases such as heart disease and cancer) in the skin of potatoes found also effective antioxidants, chlorogenic acid. Potato skin is also proven to increase hemoglobin levels (Khomsan, A, 2009). However, that does not mean potato skin does not have adverse side effects, if the use is not appropriate. In order to use it optimally, you need to know enough information about the advantages and disadvantages and the possibility of abuse. However, the level of toxicity from potato skin is still unknown. Brine Shrimp Lethality Test (BSLT) is one of the oxicity test methods that is widely used in tracing bioactive compounds that are toxic from natural materials. This research is an experimental research with post test-only control group design approach. Treatment with giving potato skin juice to Artemia salina Leach larvae with the aim to determine the potential of plant biological activity based on the toxicity of secondary metabolites contained in it, and at the same time as the initial screening test of anticancer activity of chemical compounds in potato skin juice. The results showed the LC50 value was at a concentration of 0.5% to 1% and based on the results of SPSS 21 analysis with probit analysis obtained LC50 price data of 0.746 gr / ml (7460 μgr / ml), based on the results of research and data analysis it can be concluded that potato skin has an LC50 value at a concentration of 0.746 gr / ml (7460 µgr / ml). With an LC50 price of more than 1000 µg / ml according to the BST method the potato skin does not have the potential for toxicity so the skin of the potato has the potential as an alternative to increasing hemoglobin levels.Keywords: potato skin, toxicity, BSlT

Pemeriksaan Jumlah Kapang Sebagai Indikator Kualitas Terasi Di Pasar Tambaksari Surabaya Dita Artanti; Fitrotin Azizah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

The main problem that occurs in the community is the lack of awareness of the importance of hygiene during the manufacture and storage of shrimp paste. This can trigger one of the contaminations originating from microorganisms including mold. The aimed of this study was to determine the number of molds as an indicator of shrimp paste quality in the Tambaksari market in Surabaya. This research is descriptive. The population of this research is shrimp paste traders in Tambaksari market in Surabaya. The research variable is the number of molds in the shrimp paste in the packaging without a brand in the Tambaksari market in Surabaya. Data on the number of molds was tabulated and analyzed descriptively by calculating the percentage of samples that were of good quality and not good. The results of the research on the number of mold colonies on shrimp paste sold in the Tambaksari market in Surabaya showed that the number of mold colonies in shrimp paste samples was safe to consumed because the amount was <2 x 102 colonies / gr, the average percentage of all shrimp paste samples was 100% good quality. The highest growth rate of mold colonies was 19 x 101 colonies / gr and the lowest growth rate of mold colonies was 0 x 101 colony / gr. Keywords : The Number of Mold, Shrimp paste

Analisis Kadar Trigliserida dan HDL (High Density Lipoprotein) Pada Tenaga Farmasi di Apotek Indobat Denpasar A.A. Sagung Mas Indrayani
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

AbstractNight shift can cause health problems one of which is the body's circadian rhythm disturbance which can lead to increased triglyceride levels and decreased levels of HDL (High Density Lipoprotein). This study to find out differences in triglyceride and HDL levels in pharmacist before and after night shift in Apotek Indobat Denpasar. This study method is pre-experimental one group pretest-posttest design. Blood collection was carried out on 28 pharmacists before and after the night shift. Triglyceride levels were measured using the GPO-PAP (Glycerol Peroxidase Phosphate Acid) method and HDL measured using the CHOD-PAP (Cholesterol Oxidase Diaminase Peroxidase Aminoantipyrine) method with a chemistry analyzer. The average triglyceride levels before the night shift were 87.05 ± 0.53 mg/dL, and levels after the night shift were 105.77 ± 0.50 mg/dL with a percentage increase in triglyceride levels was 24.66%. The average HDL levels before the night shift were 59.84 ± 0.47 mg/dL, and the levels after the night shift were 48.61 ± 0.42 mg/dL with a percentage decrease in HDL levels was 27.24%. Based on the results of the study, the night shift can increase triglyceride levels and decrease HDL levels. Keywords: chemistry analyzer, CHOD-PAP, HDL, GPO-PAP, pharmacist, triglycerides.

EFEKTIVITAS BUNGA WIJAYA KUSUMA (Epiphyllum oxypetalum) TERHADAP PENURUNAN KADAR ASAM URAT TIKUS WISTAR rahayu artini yuan
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 2, No 2 (2019): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

ABSTRACT The isolation of wijaya kusuma (Epiphyllum oxypetalum) flower was carried out in this study. Methanol was applied to maceration an 27 g sample of extract its was produced from 250 g of sample powder. In vivo test results, methanol extract of wijaya kusuma flower (Epiphyllum oxypetalum) dose of 100 mg / kg BB, 200 mg / kg BB, and 400 mg / kg BW can decreasing uric acid concentration. From this research, it was obtained that the best dose of extract of wijaya kusuma flower (Epiphyllum oxypetalum) applied to decrease uric acid was 400 mg/Kg BW with a decrease percentage of 63,50%. The probit test results obtained the value of ED50 methanol extract of wijaya kusuma flower (Epiphyllum oxypetalum) of 221 mg / Kg BB. In this study it can be concluded that soursop leaf extract (Annona muricata L.) can reduce uric acid levels in research mice. For future research, this method could be applied to human in decreasing uric acid concentration in blood.Keywords: kusuma wijaya flower (Epiphyllum oxypetalum), extraction, maceration, and uric acid.

Perbedaan Pertumbuhan Bakteri Staphylococcus aureus Dengan Pemberian Perasan Daun Anting-Anting Dan Perasan Daun Bahagia Dita Artanti; Eka Radiawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3 No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

ABSTRACTÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Gram Positive Bacteria, one of which is Staphylococcus aureus is a type of bacteria that is a major cause of nosocomial infection due to surgical procedures and treatment equipment in hospitals and poisoning in several regions in Indonesia. Staphylococcus aureus is commonly found in skin, nose, mouth, eye, finger, intestinal, and liver lesions. Juice of Anting-anting leaves (Acalyhpa indica L.) and happy leaf plants (Dieffenbachia bowmanii) are known to have medical benefits. Especially in its ability to produce metabolites that function as anti-bacterial compounds. This study aims to determine the differences in the growth of Staphylococcus aureus colonies which were given the leaves of Anting-anting and the leaves of Happy leaves. The research method uses the liquid dilution method in which the Staphylococcus aureus bacteria is incubated with the leaves of the Anting-anting and the leaves of the Happy leaves with a concentration of 100% at 37 Â° C overnight. Then grown on Mannitol Salt Agar (MSA) media. The results showed that there was no difference in the growth of Staphylococcus aureus bacteria colonies that had been given the treatment of Juice of Anting-anting leaf (Acalyhpa indica L.) and happy leaf plants (Dieffenbachia bowmanii) which were marked by the absence of colony growth. So that the concentration of 100% in both leaves of juice is the best concentration that can be used to control the growth of Staphylococcus aureus.Â Â Keywords : Anting-anting Leaves, Bahagia Leaves, Staphylococcus aureus

Hubungan Paparan Pestisida Terhadap Kadar Trigliserida Pada Petani Di Daerah Mojokerto Andreas Putro Ragil Santoso; Devyana Dyah Wulandari; Devyani Dyah Wulansari
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Alzheimer’s chronic generational neuro disease is indicated by the loss of neurons and synapses, factors that cause Alzheimer’s include cholesterol such as triglycerides and poisoning due to pesticides due to pesticides due to spraying activity. This study aimed to determine the extent of pesticide exposure to triglyceride levels in farmers in the Mojokerto area. This research is an observational type of research with an experimental approach. This research was conducted in Sumbersono Village, Dlanggu Subdistrict, Mojokerto Regency with 25 farmers who carried out spraying activities. This study uses a correlation test that is the Spearman's test with a confidence level of p < 0.05. Based on results of the study showed that farmers who carried out pesticide spraying activities with a working period of up to more than 15 years with a long time under the field around 3-6 hours but did not use personal protective equipment properly. The results of the average triglyceride levels of 158.2 mg / dL Spearman's test showed triglyceride hash to work mas amounted to 0.899, to the length of work of 0.442 and to the use of PPE 0.811 it showed p > 0.05 which means there was no relationship between farmers who carried out spraying activities with triglyceride levels.Keyword: Farmer, Spraying activity, Triglycerides

Status Kadar Kolesterol Terhadap Kebiasaan Orang Yang Mengonsumsi Teh Nur Vita Purwaningsih
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

ABSTRACT Lifestyle must be a trend nowadays, many high cholesterol foods needed in daily life can increase blood cholesterol and increase the risk of cardiovascular disease. One way to reduce cholesterol in the body is by consuming tea. Tea has a very beneficial content for the body. One of the contents of the tea is catechins. Catechins are secondary metabolites that are naturally produced by plants belonging to the flavonoid class, which are made to help absorb calcium in the intestine. This type of research is observational analytic samples collected are students who have the habit of consuming tea by doing two examinations that is before and observing observations for 3 months. Based on the results of the study obtained an average cholesterol level before the observation of the results obtained 152.9 mg / dl while for the temporary examination of the results obtained 141.3 mg / dl. The results of data analysis using the Paired T Test with sig 0.00 which means less than 0.05. So, it can be concluded that there is an influence of tea consumption habits on cholesterol levels. Keywords: cholesterol, habits in consuming tea

Perbandingan Hasil Pemeriksaan Glukosa Darah Dengan Menggunakan Serum Dan Plasma Natrium Fluorida (NaF) Di Laboratorium Klinik Medika Jayapura Herlando Sinaga; Cuti Irianti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

The purpose of this research was to compare the results of examination of blood glucose levels using serum and plasma Sodium Fluoride (NaF). The population used in this study were all patients who came to check blood glucose while at the Medika Clinic Laboratory. The number of samples in this research was 67 (total population). The method used in examination of blood glucose is the enzymatic method of Glucose Oxidase (GOD - PAP). This research was conducted for 1 month, from April 1 to April 30, 2017. To compare the values between serum and plasma sodium fluoride (NaF) specimens, it was analyzed statistically using the Social Science Stastistial Program (SPSS) for windows version 21 with the test method Mann Whitney and obtained a mean of 154.49 mg/dl in serum specimens and 145.36 mg/dl in plasma specimens of sodium fluoride (NaF). The results of statistical tests of blood glucose levels with serum and plasma NaF specimens showed no significant difference and obtained a significance value of 0.196 which means a probability >0.05. The conclusion of this research is that there is no significant difference between the examination of blood glucose using serum specimens and plasma Sodium Fluoride (NaF).Keywords : Blood Glucose, Serum, Sodium fluorida (NaF)

Validasi Dan Verifikasi Hasil Uji Sun Protection Factor (Spf) Pada Sediaan Sunblock Dan Sunscreen Bermerk Dengan Metode Spektrofotometri rahayu artini yuan
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Tests for the determination of SPF value were calculated from the seven sunblock and brandes sunscreen preparation which were analyzed using 96% etanol solvent and kloroform, ranging from 17.21-26.88. The results of the VA-14 sample calculation have the lowest SPF value (SPF = 17.21) and the ES-30 sample has the largest SPF value calculation results from the seven samples (SPF ES-30 = 26.88). The seven samples are samples that have well-known brands in Indonesia. Of the seven cosmetics samples, six samples had a% recovery in accordance with the standard between 80-120%, but one product, namely SA-35, had a recovery rate of 73.23%. Organoleptic test results showed that the overall type of cream was m / a, fragrance, color from milky white, light brown, and light green. The pH value between 6.8-7.5 according to the quality standard of sunscreen preparations in SNI 16-4399-1966, the pH value for sunscreen preparations is 4.5-8.0. Thus, the results of the seventh pH of the sample are still within good standard limits for cream preparations containing sunscreen.

Gambaran Parasit Cacing Nematoda Usus Dan Cestoda Pada Feses Sapi (Boss sp.) Di Peternakan Sumber Jaya Ternak, Kecamatan Tikung, Kabupaten Lamongan, Jawa Timur Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Cows are important animals for Indonesian farmers who have high economic value. Cow breeding business has many benefits for human life, especially meat, milk, bones, skin, offal and feces (cow dung). Cow feces can be used as an organic fertilizer manufacture by cattle farmers. But it needs to be considered in making organic fertilizer derived from cows because it contains many diseases and parasites. If the cow's feces are not treated properly, it will cause environmental pollution to the local residents. Cow infected with parasites can experience a decrease in body weight, decreased endurance, impaired growth and death. To find out intestinal helminth parasitic infections, one of them is by identifying worms in cow feces. The majority of the population of Tikung Subdistrict, Lamongan Regency, earn their living as farmers and ranchers. The purpose of this study was to identify intestinal Nematode worms in cow feces in Tikung Subdistrict, Lamongan Regency. The method used in this study was Nacl Saturated to determine the morphology and forms of parasites. The results showed that cattle feces samples taken from the Tikung Subdistrict of Lamongan District contained the Nematoda parasite, which was found the presence of Hookworm eggs in 3 samples with a percentage of 6% and found 1 positive sample (+) containing Taenia saginata eggs, with a percentage of 2% of the total 50 samples in Sumber Jaya Livestock Farm in cattle on Tikung Subdistrict, Lamongan Regency is still relatively low. Keywords: Cow Feces (Bos sp.), Intestinal Nematodes, Cestodes

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