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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
ISSN : 20895690     EISSN : 24069272     DOI : -
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
CORRELATION OF DEMERIT POINT SCORE (DPS) AND CHEMICAL PARAMETERS ON QUALITY OF FRESH ESTUARY GROUPER (Epinephelus tauvina) DURING STORAGE IN ICE Farida Ariyani; Yusma Yenni; Rudi Riyanto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 2 (2014): August 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i2.106

Abstract

Quality changes of fresh estuary grouper (Epinephelus tauvina) fish kept in ice was evaluated for sensory and chemical parameters during 21 days of storage. Fish was killed by  immersing in ice water (hypothermia). Fresh fish was placed in insulated boxes, and covered with ice flake. The ratio of ice and fish was 2:1 (w/w) and the boxes were kept at ambient temperature.  Evaluation was conducted every 3 days for sensory characteristics by Demerit Point Score /DPS for appearance, eyes, gills, belly, vent, and belly cavity. Moreover, Total Volatile Base/TVB, Trimethyl Amine/TMA , and the K value were measured until fish was rejected by panelists. The results showed that estuary grouper fish stored in ice was rejected by panelists on 21 days of storage with TVB value of 29.43 mgN%, TMA value of 22.10mgN% and K value of 40.54% (max level of 60%). Related to correlation between sensory and chemical parameters, it showed that Demerit Point Score correlated well with TVB, TMA and K value.
Prevalence of Aflatoxin B1 in Commercial Dried Fish from Some Regions of Java Ninoek Indriati; Irma Hermana; Izhamil Hidayah; Endang Sri Rahayu
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.290

Abstract

The purpose of this study is to study the prevalence of aflatoxin B1 in commercial dried salted fish and other related information. A total of 150 samples were classified into 3 groups, based on the salt content. Low salt content (0-5%) were dried anchovies (Stolephorus sp.) and commerson’s anchovy (Stolephorus commersonii); moderate salt content (6-10%) were medan anchovy (Stolephorus bataviensis) and  whipfin silverbiddy (Gerres filamentosus) while high salt content (10%) were moonlight gouramy (Trichogaster microlepis) and snakehead fish (Channa striata). The samples were collected from different seller in Java Island and then determined for Aspergillus flavus, Aflatoxin B1, salt content, moisture content, pH, water activity and total mold count. Results showed that dried salted fish were contaminated with A. flavus at temperature of 25.2-32.2 oC, 65-84% humidity, 17-50% moisture content, 0.25-19.88% salt content, and 0.73-0.86 aw. The prevalence of A. flavus in dried salted fish was 9.33% (14/150) and the prevalence of aflatoxin B1 was 8% (12/150) with detectable concentrations of 10.71-33.6 ppb.
Algal Lectins and their Potential Uses Praseptiangga, Danar
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): August 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i2.125

Abstract

Lectins (hemagglutinins), or carbohydrate-binding proteins, are ubiquitous in nature and play important roles in many biological processes. They bind mono- and oligosaccharides reversibly with high specificity, but are devoid of catalytic activity, and in contrast to antibodies, are not products of an immune response. The erythrocyte agglutination or hemagglutination activity of lectins is a major attribute of these proteins and is used routinely for their detection and characterization. Due to their biochemical and biological properties, lectins attract a great deal of attention in the fields of medicine, molecular biology, biochemistry, and glycobiology. Lectins have been isolated and characterized from marine algae. Many of algal lectins generally have common characteristics of low molecular weight, no divalent cations requirements for their activity, and have an affinity for glycoproteins but not for monosaccharides. These properties imply that they may possess molecular structures and carbohydrate-binding specificities distinct from known lectins from other sources. Recent investigations revealed that algal lectins have the strict binding specificity to some definite oligosaccharide structures and are grouped into several types on the basis of oligosaccharide-binding specificity. Thus, marine algae are promising sources of novel lectin molecules for basic research and application. In spite of the progress made in biochemical characterization of algal lectins, additional information are still needed for a more comprehensive understanding of their molecular structures and possible biological functions for the future applications.
Chemical Composition and Fatty Acid Profile of Some Indonesian Sea Cucumbers Yusro Nuri Fawzya; Hedi Indra Januar; Rini Susilowati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.118

Abstract

Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was  intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca),  one  of  sea  cucumbers  producing area  in Indonesia. The samples coded as  H-03, H-04, H-05 and H-10, were identified as Bohadschia  argus, Holothuria  fuscogilva, Thelenota  ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA.  Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49–4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of w6/w3 fatty acid was 1.2–3.2 showing that total w6 fatty acid was higher than total w3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.
PROTEIN DARI BIOTA LAUT DAN POTENSINYA DALAM INDUSTRI YANG MENGGUNAKAN TEKNOLOGI NANO-SILIKA Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 2, No 2 (2007): December 2007
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v2i2.137

Abstract

Peran protein dalam proses pembentukan biosilika yang terjadi di dalam tubuh beberapa biota laut telah menginspirasi peneliti untuk mempelajari dan meniru teknologi tersebut untuk aplikasi industri. Dengan proses biologi biasa, protein dari spikula spons dan dinding sel diatom dapat berfungsi menjadi cetakan dan mendirect proses biosilika berskala nano. Isolasi dan identifikasi protein tersebut, yang dilanjutkan dengan uji coba proses biosilika secara in vitro, memperlihatkan potensi protein tersebut sebagai biokatalis/agen biologi dalam sintesis silika. Sintesis silika secara kimia, seperti dalam produksi bahan resin, katalis, dll, selama ini dikenal merupakan proses yang tidak ramah lingkungan dan boros energi (diperlukan suhu, pH dan tekanan tinggi). Karena itu, eksplorasi dan riset lebih lanjut mengenai protein ini dirasa sangat penting mengingat bahan dasar silika, termasuk didalamnya teknologi nano, telah dipergunakan dalam berbagai bidang industri termasuk diantaranya industri pangan, elektronika, otomotif, dan lain-lain. Perkembangan riset tentang protein ini, baik yang telah dilakukan oleh peneliti luar negeri maupun yang dilakukan di Indonesia, serta aplikasi teknologi nano-silika akan disampaikan pada makalah ini.
Back Cover Squalen Bulletin Vol. 11 No. 1 Tahun 2016 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.328

Abstract

Production and characterization of bacteriocin produced by lactic acid bacteria isolated from rusip Arifah Kusumarwati; Ninoek Indriati; Irma Hermana
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): May 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i1.77

Abstract

Research was conducted to produce and characterize bacteriocin produced by lactic acid bacteria (LAB) isolated from rusip, a traditional Bangkanese fermented fish product. Experiment was started by isolation of lactic acid bacteria from rusip, followed by screening to obtain the best isolate which has the highest bacterial inhibition activity. The selected isolate was then identified and used to produce crude bacteriocin. The crude bacteriocin was characterized through its stability in high temperature and proteolytic enzymes, inhibitory spectrum, pH sensitivity and effect of surfactants. The result showed that CN1.10a isolate which was identified as Lactococcus lactis subsp lactis has the highest bacterial inhibition activities; therefore it was selected to produce crude bacteriocin. The bacteriocin produced was heat stable, sensitive to proteolytic enzymes i.e. proteinase-K and papain but not to RNase. It inhibited Escherichia coli, Listeria monocytogenes and Lactobacillus plantarum. It stable at pH 2.0 to 6.0. Among surfactans used sodium dodecyl sulphate (SDS), lauryl sarcosine and EDTA were able to stimulate bacteriocin production, while the production were strongly inhibited by Tween 20, Tween 80, Triton X-100 and urea. Based on the above characteristic, the bacteriocin was suitable to be used as a preservative of food which has to be processed at high temperature.
Front Cover Squalen Vol 11 no 2 h, erki
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AN EMERGING MARINE BIOTECHNOLOGY: MARINE DRUG DISCOVERY Nurrahmi Dewi Fajarningsih
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 2 (2012): August 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i2.19

Abstract

Marine natural resources offer an opportunity to discover a novel chemical diversity withinterest ing pharmacologically active compounds to treat many diseases such as cancer,inflammation, bacterial and parasitic infections, and many other diseases. Marine drug discoveryis a rising area in marine biotechnology. Several hits of marine-derived drug compounds wereapproved; two of them are Ziconotide and Trabectedin. In 2004, Ziconotide was approved as paintreatment drugs in the United States and Europe. Then, in 2007, Trabectedin was also approvedas anticancer drug in Europe. The main problem in marine drug discovery research is materialsupply problem. Up till now, strategies to overcome the problem are “Pharmaceutical aquaculture”of biologically active marine biota and chemical synthesis approach. Chemical synthesis approachis feasible solution to be used, especially when working with less complex structure of compounds.However, when working with structurally complex compounds where total or even semi synthesiswas very difficult to be provided, aquaculture can be a solution. Currently, the use of microbiology,biochemistry, genetic, bioinformatics, genomic and meta-genomic has been intensifying in orderto have a better result in marine natural product drug discovery. As chemical synthesis needs anexpensive investment of advanced technology and highly skilled human resources, thuspharmaceutical aquaculture is more practicable to overcome the material supply insufficiency inIndonesia. Up till now, many Indonesian marine bioprospectors have been working with culturablemarine microorganism to produce bioactive compounds and some others starting to work withgenomic and metagenomic-based drug discovery.
Implementasi Sistem Ketertelusuran pada Produk Perikanan Dwiyitno, Dwiyitno
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 3 (2009): December 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i3.155

Abstract

Ketertelusuran adalah kemampuan suatu sistem untuk mengenali dan menelusuri suatu produk pada setiap tahapan produksi, pengolahan maupun pemasaran. Untuk produk pangan, tujuan utama sistem ketertelusuran adalah untuk mencatat/mendokumentasikan produk mulai dari bahan baku sampai kepada konsumen (from farm to fork). Aplikasi sistem ketertelusuran sangat diperlukan guna menghasilkan produk-produk yang terjamin mutu dan keamanannya. Pada prinsipnya, terdapat  2 aspek penting pada implementasi sistem ketertelusuran, yaitu tracking dan tracing, yang digunakan sebagai alat untuk menelusuri riwayat produksi suatu produk. Pada  prakteknya,  implementasi  sistem ketertelusuran  pada produk perikanan di Indonesia masih terbatas pada produk hasil industri maupun produk ekspor. Komitmen bersama antara pemerintah, pelaku usaha perikanan/produsen, dan konsumen sangat penting dalam membangun sistem ketertelusuran  pada produk perikanan, termasuk pada unit usaha kecil dan menengah.

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