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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
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PENGGUNAAN RETORT POUCH UNTUK PRODUK PANGAN SIAP SAJI Murniyati, Murniyati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): August 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i2.148

Abstract

Retort pouch adalah kemasan fleksibel berbentuk pouch atau kantong yang digunakan untuk mengemas produk pangan siap santap (Meal Ready to Eat). Kemasan tersebut dibuat dari laminasi aluminium foil dan polimer, tahan terhadap proses sterilisasi. Pengemas retort pouch telah banyak digunakan pada produk sterilisasi ikan untuk menggantikan kaleng. Pengemas semacam kaleng harganya relatif mahal, sehingga menyebabkan harga produk yang dikemas dengan pengemas tersebut menjadi mahal. Dengan berkembangnya retort pouch processing, proses menjadi lebih efisien, produk bisa dipanaskan terlebih dahulu dengan memasukkan ke dalam air mendidih sebelum dikonsumsi. Retort pouch dengan bentuk yang tipis memungkinkan untuk mengurangi waktu pemanasan, dengan demikian dapat menghindari over cooking. Produk yang dihasilkan mempunyai warna yang lebih baik, tekstur kompak, dan tidak terjadi susut gizi. Produsen dapat mengurangi energi yang diperlukan untuk produksi retort pouch dibandingkan dengan kaleng. Selain beberapa keunggulannya, retort pouch juga memiliki kelemahan atau kendala. Kendala yang utama adalah modal yang besar untuk penyediaan mesin khusus dan proses pengisian yang lebih lambat dan kompleks dibandingkan dengan kaleng.
Back Cover Squalen Bulletin Vol. 13 No. 1 Tahun 2018 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.349

Abstract

Identification of SGS 1609 Cellulolytic Bacteria Isolated from Sargassum spec. and Characterization of The Cellulase Produced Yusro Nuri Fawzya; Stenny Putri; Nita Noriko; Gintung Patantis
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i2.87

Abstract

Bacterial isolate from seaweed designated as SGS 1609 was previously found to be able to produce cellulase represented by formation of clear zone on solid medium containing carboxymethylcellulose (CMC). This research was conducted to identify the isolate and determine optimum production time as well as characterize the cellulase produced. The isolate was identified using  16s-rRNA gene analysis. Cellulase production was conducted by cultivating the isolate in the liquid medium containing CMC followed by centrifuging to get supernatant as the crude enzyme. The enzyme was then concentrated using ammonium sulfate precipitation and ultra filtration. The concentrated enzyme having higher activity produced from the concentration process was then characterized  to determine its optimum pH and temperature, heat stabilization, metal ions effect and substrate specificity. The result showed that the SGS 1609 isolate was identified as Serratia marcescens with 99%  similarity. The isolate produced cellulase optimally at 4 days incubation. Ultra filtration produced higher enzyme activity compared to NH4-sulfate precipitation. The enzyme concentrated by ultra filtration worked optimally at the  pH of 7, temperature of 50 oC, stable at the temperature of 60 oC for 240 minutes and was increased its activity by Ca2+ and Mg2+ ions. On the other hand, the enzyme was inhibited by Fe3+, Zn2+ and Na+ ions, but was not relatively affected by K+ and EDTA. The use of conventional agar producer waste  treated with 6% NaOH gave highest activity compared to other substrates.
Metagenomics-Based Cloning of Amilase-Encoding Genes from the Uncultured Symbiotic Bacteria of a Marine Sponge Theonella swinhoei from Kapoposang Island, South Sulawesi Priyono, Franciscus Edi; Zilda, Dewi Seswita; Kusnadi, Yudi; Hadi, Tri A; Nurrachmi, Irvina; Uria, Agustinus Robert
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.272

Abstract

Marine sponges have recently been recognized as the source ofenzymes, including members of hydrolases. Hydrolytic enzymes are extracellularly produced by sponge-associated bacteria to mediate the metabolism of complex organic matters, thereby assisting the sponge hosts in nutrition and metabolic processes. Among hydrolytic enzymes, amilaseshas attracted increasing attention due to their potential industrial applications. This research work was aimed atutilizing functional metagenomicsapproach for the discovery of amilases derived from the uncultured symbiotic bacteriaof the Indonesian marine sponge Theonella swinhoei. Weinitially constructed a small-insert metagenomiclibrary in Escherichia coliby cloning of metagenome in the size range of5-20 kb prepared from the sponge’s microbiome. Further functional screening of the resulting metagenomic library led to the isolation of two recombinant E. coli clones potentially harboring amilase genes, as indicated by the presence of clearing zones surroinding the selective medium containing 1% amilum. 
Biopreservative nisin: its application to fishery products Yusro Nuri Fawzya
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 3 (2010): December 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i3.50

Abstract

The need for safe food products amid increasingly perceived widespread use of various foodadditives which are sometimes hazardous to health. Preservative is one of food additives intendedto extend the shelf life of food products. The preservative produced by lactic acid bacteria isbacteriocin. Nisin, one of bacteriocin produced by LAB Lactococcus lactis,is considered safeand allowed its use in many countries, including Indonesia. This preservative is widely used forfood products due to its ability in inhibiting bacteria, especially Gram-positive bacteria such asClostridium botulinum, Staphylococcus aureus, Streptococcus hemolyticus, Listeriamonocytogenes, Bacillus stearothermophilus, and Bacillus subtilis.Nisin works actively at lowpH and can be used singly or in combination with other preservation treatments. Based on itsphysico chemical properties and bioactivity, the use of nisin in fishery products is more suitable infermentation process as well as fish canned in acidic medium such as tomato sauce.
Produksi Mikroalga sebagai Biofuel Basmal, Jamal
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 3, No 1 (2008): June 2008
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v3i1.168

Abstract

Biofuel  yang berasal dari  tanaman  darat  atau  laut  seperti mikroalga  sudah mulai  diupayakanuntuk menjadi  energi  alternatif  pengganti minyak  bumi. Berdasarkan  hasil  penelitian,  diketahuibahwa minimal  14  spesies mikroalga  berpotensi untuk menghasilkan biofuel dengan  kandunganminyak  antara  15–77%  dari  bobot  kering mikroalga. Untuk menghasilkan  1  kg  (bk)  biomassadiperlukan media  tumbuh  air  laut  sebanyak  1m3. Di  samping  kandungan minyaknya  dapatdiekstrak,  limbah  padatnya  juga  dapat  dipergunakan  untuk  pakan  ternak  atau  bahan  bakufermentasi  untuk menghasilkan  biomethane.  Tantangan  yang  dihadapi  dalam meningkatkanproduksi  biomassa mikroalga  adalah  ketersediaan  karbon  dioksida. Selain  itu,  dalam  prosesekstraksi  juga  diperlukan  suatu  alat  yang  dapat memecah  dinding  sel mikroalga  sehinggakandungan minyaknya  dapat  diekstrak  semaksimal mungkin.
Distribution of brown seaweed producing alginate in Indonesia and the potential utilization Subaryono Subaryono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 2 (2011): August 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i2.61

Abstract

Brown seaweed producing alginate frequently obtained in Indonesian waters , especially fromgenus of Sargassum and Turbinaria. These seaweeds are wide distributed from Aceh to Papuawaters. There are 15 species of Sargassum and some species of Turbinaria in Indonesia. Thisseaweed were very potential used as a row material of alginate industry, with main market Chinaand Malaysia. Quality requirement for seaweed as row material for alginate industry in China werealginate content not less than 17%, viscosity 47 cP, and gel strength 139 g/cm2. Expansion ofalginate industry in Indonesia was limited by low continuity of row material production. Prospectiveutilization of this brown seaweed as a row material of drug/traditional herbal medicine were sohigh because its ability to reducing high blood glucose in diabetes patient and decelerate thegrowth of cancer cell. Natural seaweed collection were done at peak season production on dryseason, and help out to increase the welfare of seaweed collection farmer.
Logic Structure Determination (LSD) as a Computer Assisted Structure Elucidation (CASE) for Molecular Structure Determination of Cytotoxic Cembranoids from Soft Coral Januar, Hedi Indra; Zamani, Neviaty Putri; Soedharma, Dedi; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.177

Abstract

Indonesian tropical soft corals are valuable resources that produce pharmacological cytotoxic cembranoids. However,  the manual structure determination in these compounds requires adequate knowledge of organic chemistry. This study presents the application of Logic Structure Determination (LSD) as a freeware Computer Assisted Structure Elucidation (CASE) for automatic molecular structure determination of cembranoid compounds from soft corals species. 12 Nuclear Magnetic Resonance (NMR) datasets of cytotoxic cembranoids were used to evaluate the accuracy of LSD in generating the possible structures. The results of this study shows that LSD generated numerous possible molecular structures as the data input files were only derived from 2D-NMR HMQC (Heteronuclear Multiple-Quantum Correlation), COSY (Homonuclear Correlation Spectroscopy), and HMBC (Heteronuclear Multiple Bond Correlation). The accuracy was significantly enhanced (only 2-4 possible cembranoid structures from each NMR dataset) with the addition of H2BC (Heteronuclear 2 Bond Correlation) experimental data. This may indicate that VLRC (Very Long-Range Correlations) significantly affects LSD capability. Furthermore, LSD with direct 2 bond NMR experimental data is a reliable CASE technique for cembranoid compounds identification. In general, this freeware-CASE has the potential to be applied  on other types of small molecule compounds and may serves as a solution for elucidation bottleneck step in studies on Indonesian natural products.
FUCOIDAN FROM BROWN SEAWEED AND ITS BIOACTIVITY Ellya Sinurat; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.8

Abstract

Fucoidan is a polysaccharide which  substantially consists of L-fucosa and ester sulphate group and is mainly contained in brown seaweed. For the past ten years, bioactivity studies of fucoidan has been conducted. Recently, fucoidan has been examined for its application in drugs. In a couple of years, fucoidan structure was succesfully identified and its bioactivity was revealed. Fucoidan exhibits various bioactivities such as anticoagulant, antioxidant, anticomplementary, anti-inflamation, gastric protector, and blood lipid level control. This review gives some brief progress in isolation and bioactivity study of fucoidan from brown seaweeds.
PENGEMBANGAN DRUG DISCOVERY DARI BAHAN ALAMI LAUT UNTUK IMUNOTERAPI KANKER Dewi, Ariyanti Suhita
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 3 (2009): December 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i3.152

Abstract

Penyakit kanker adalah permasalahan kesehatan global yang mengancam setiap individu baik di negara maju maupun di negara berkembang. Penanganan kanker sejauh ini dilakukan berdasarkan metode konvensional yang cenderung menurunkan kualitas hidup para penderitanya. Pendekatan imunoterapi kanker adalah suatu metode pengobatan kanker dengan cara menstimulasi sistem kekebalan hidup pasien untuk melawan tumor melalui regulasi enzim-enzim yang berperan penting pada jalur molekuler tertentu dengan tujuan untuk meminimalisasi terjadinya efek samping yang tidak diinginkan yang umumnya terjadi pada terapi konvensional. Beberapa target molekuler telah dikembangkan saat ini, diantaranya adalah penghambatan enzim Indoleamine-2,3-dioxygenase  (IDO)  dan  pengaktifan  enzim  SHIP (SH2-containing-inositol-5-phosphatase). Senyawa-senyawa bioaktif dari bahan alam laut yang telah dilaporkan sebagai regulator kedua enzim di atas akan dijabarkan.

Page 14 of 37 | Total Record : 363

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