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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
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CARAGEENAN OLIGOSACCHARIDES : BIOLOGICAL ACTIVITY AND ITS DEVELOPMENT OPPORTUNITIES IN INDONESIA Subaryono, Subaryono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.347

Abstract

Oligosaccharides from hydrocolloids especially carrageen now get a lot of attention because of the abundant raw material availability and various biological activities so it is potentially used in various fields both in the field of food and non food. Like oligosaccharides of terrestrial materials, carageenan oligosaccharides have many biological activities such as antibacterial, antiviral, antioxidant, immunomodulatory, antiinflamatory, anticancer, and other biological activity. Oligosaccharide carrageen can be produced either physically, chemically or enzymatically. This paper reviews articles on oligosaccharide carageenan, how it is produced, biological activity and development opportunities in Indonesia.
CYTOTOXIC SATURATED FATTY ACIDS FROM THE INDONESIAN SEA CUCUMBER Holothuria sp. Hedi Indra Januar; Muhammad Nursid; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 1 (2014): May 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i1.69

Abstract

Sea cucumbers are one of Indonesia’s marine organism with the potential to be developed as possible herbal medicines. In a preliminary study of cytotoxic activity of ethanol extracts of 14Indonesian sea cucumber species, the most active extract came from the Holothuria sp. Thecurrent research aimed to identify the major cytotoxic component in the sea cucumber Holothuriasp., that yielded the most active extract. The samples were collected from South Lampung beach.Isolation of the cytotoxic component was done employing liquid flash and preparative reversedphase (C 18) chromatography. Cytotoxic ity evalu atio n of fractio ns collected durin g thechromatography was conducted using the MCF-7 tumor cell line. Elucidation of the structure ofmost active isolate was done by NMR (Nuclear Magnetic Resonance) spectroscopy and GC-FID(Gas-Chromatography-Flame Ionisation Detector) analysis. The results of these analyses showedthe most active compound to be stearic acid; IC 50towards MCF-7 cells 10.32 ppm.
Evaluation of Non-Saccharomyces Cerevisiae Strains Isolated from Sea Water Against Inhibitory Compounds for Ethanol Production Thontowi, Ahmad; Putra, Filemon Jalu Nusantara; Yopi, Yopi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i2.284

Abstract

An important parameter in industrial bioethanol fermentation is the resistance of yeast to osmotic pressure and inhibitor compounds. Aureobasidium pullulans LBF-3-0074 and Schwanniomyces etchellsii LBF-3-0034 are reported capable to produce ethanol. LBF-3-0034 and LBF-3-0074 are yeast strains isolated from Bali and Lombok sea water. This study aimed to evaluate characteristics of both LBF-3-0034 and LBF-3-0074 strains under the effects of glucose and inhibitor compounds. Both strains were allowed to consume glucose up to 120 mM. Then, these strains were grown with the present of several inhibitors, i.e. 5-hydroxymethyl-2-furaldehyde (5-HMF), furfural, acetic acid, formic acid, and levulinic acid. Results showed that the two yeast strains studied could grow and ferment the sugars under both osmotic and inhibitor stress conditions. As conclusion, Schwanniomyces etchellsii LBF-3-0034 and Aureobasidium pullulans LBF-3-0074 are potential for direct fermentation of lignocellulosic hydrolysate to ethanol.
Chitosan nanoparticles: process and their application as drug delivery. Hari Eko Irianto; Ijah Muljanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 1 (2011): May 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i1.55

Abstract

Chitosan has wide ranges of potential applications and Indonesia possesses abundant rawmaterial sources such as crab and shrimp shells to produce it. Chitosan have beneficial properties,i.e. biocompatible, biodegradable, non-toxic and inexpensive. Nowadays, several experimentson the preparation of chitosan into nanoparticles and its utilization as drug delivery for varioustargeted therapies have been conducted. Chitosan nanoparticles can be prepared using variousmethods, e.g. emulsion cross-linking, precipitation, spray drying, emulsion-droplet coalescencemethod, ionic gelation, reverse micellar method and polyelectrolyte complex. Applications ofchitosan nanoparticles as drug delivery can be performed through diverse administrations includingparenteral, peroral and ocular, non-viral gene delivery vectors, vaccine delivery, and photodynamictherapy.
IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STAR FISH (ACANTHASTER PLANCI) ON LYMPHOCYTE PROLIFERATION IN-VITRO M. Janib Achmad; Alim Isnansetyo; Noer Kasanah; Ustadi Ustadi; Kamiso Kamiso
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 3 (2014): December 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i3.109

Abstract

The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88% (160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml) increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that star fish A. planci from Ternate Island has a potential source of immunostimulator.
Fungal Community Structure of Macroalga Ulva Intestinalis Revealed by Miseq Sequencing Agusman, Agusman; Danqing, Feng
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.315

Abstract

Since algae are well known as the host of fungi, the information about total fungal community associated with alga is needed to give the initial data that is useful for natural product discovery. The study on total fungal community associated with marine macroalga U. intestinalis from Xiamen-China was carried out using MiSeq, a powerful platform for microbial ecology studies. Metagenomics DNA was isolated from surfaces of the U. intestinalis thallus and rocks colonized by this alga and  18S ribosomal RNA region was sequenced using MiSeq Illumina to yield 81360 sequences from 162720 reads, which average read length is 245.61 bp. The sequences were assigned to operational taxonomic units (OTUs), where 101 genera represent 5 phyla, 17 classes and 64 families of fungal. Ascomycota was the highest abundance share of fungal community at phylum level. Meanwhile Aspergillus, Blastobotrys, Alternaria, Knufia, and Fusarium were some of dominant genera found associated with U. intestinalis which have been reported as the promising source of natural products. By using MiSeq platform, this study revealed the total community structure of algicolous fungi associated with U. intestinalis.
Front Cover Squalen Bulletin Vol. 11 No. 3 Tahun 2016 Squalen, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.320

Abstract

Astaxanthin: Senyawa Antioksidan Karoten Bersumber dari Biota Laut Ifah Munifah; Thamrin Wikanta
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 1 (2006): December 2006
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v1i1.71

Abstract

Meskipun berbagai jenis antioksidan dalam makanan telah berkontribusi dalam pencegahan penyakit, namun sejumlah riset yang telah dilakukan lebih memfokuskan pada tiga jenis antioksidan yakni vitamin E, vitamin C dan karotenoid karena berperan penting sebagai nutrien yang diperlukan dalam metabolisme tubuh manusia.
Composition of Fatty Acids in Evaluation of Sea Cucumber Potency for Nutraceutical Product Development Susilowati, Rini; Pratitis, Asri; Januar, Hedi Indra
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.238

Abstract

Unsaturated fatty acid in food has an effect to improve human health, while saturated fatty acid has the opposite impact. Ratio between both types of fatty acids is important to evaluate the biological material for nutraceutical product development. This paper aimed to evaluate the nutraceutical potency of sea cucumber from Kendari Bay Waters, South East Sulawesi, based on their fatty acids composition. The analysis was conducted by the 1H-NMR (Hidrogen-Nuclear Magnetic Resonance) method. Several species within 4 genera (Holothuria, Bohadschia, Actinopyga, and Stichopus) were selected as the subject of the study. Comparison of fatty acid composition has been done in genera level, the preliminary study found that variation between species among the same genera was insignificant (p >0.05). Results of the study detected that Holothuria contained with an even ratio of Poly Unsaturated Fatty Acids (PUFA) and Saturated Fatty Acids (SFA), but Actinopyga was detected with low PUFA/SFA ratio. It may suggest that Holothuria from this region is the most potential raw material for nutraceutical product development. Meanwhile, precaution is needed for genera that contained with low PUFA/SFA ratio. Furthermore, comparison with other studies detected that fatty acids composition is not morphological specific, but vary, according to food viability, environmental and geographic location. Therefore, preliminary screening is important in evaluation of sea cucumber material for nutraceutical product development.  
APPLICATION OF MODIFIED ATMOSPHERE PACKAGING (MAP) ON FRESH FISH Putri Wullandari; Diini Fithriani
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 1 (2012): May 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i1.14

Abstract

Packaging has many functions, e.g. as containment, to protect products from physical damage,and from H2O, O2, and CO2 exposure, as well as to attract the consumers. Based on the technology,packaging  can  be  divided  into  3 types i.e.  passive  packaging,  active  packaging,  and smartpackaging. Modified Atmosphere Packaging  (MAP)  is the most  common  active  packaging  foundin  the market. MAP has been used  to extend  the shelf  life of several  fish such as  raw whiting  (codfamily), mackerel,  salmon  fillet, cod  fillet,  fresh bluefin  tuna  fillet,  etc. MAP which was combinedwith  freeze-chilling has been proved  to extend the shelf  life   of  raw whiting, mackerel, and  salmonfillet. MAP also  increased  cod  fillet’s  shelf  life  up  to 20  days, whiting  fillets packed  in  100% CO2and stored at 4°C temperature up to 15 days. MAP combined with antioxidant on fresh bluefin tunafillets stored at 3°C  for 18  days was able to extend products shelf  life  from 2 days  (control)  to 18days, meanwhile 100% N2 in packaging has  protective effect  on haemoglobin  and  lipid oxidation.Packaging innovations and ingenuity will continuously develop MAP  that is oriented  for consumer,enhance  the product, environmentally  responsive, friendly, and  cost effective.

Page 13 of 37 | Total Record : 363

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