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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
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Back Cover Squalen Bulletin Vol. 10 No. 2 Tahun 2015 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): August 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i2.335

Abstract

Alginate Lyases: Sources, Mechanism of Activity and Potencial Application Subaryono Subaryono; Rosmawaty Peranginangin; Maggy Thenawidjaja Suhartono; Fransiska Rungkat Zakaria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i3.39

Abstract

Alginate lyases are group of enzymes which catalyze depolymerization of alginate into oligosaccharides. Alginate lyase have been widely used in many applications such as in production of bioactive oligosaccharides, control of polysaccharide rheological properties, and polysaccharide structure analysis. The products of alginate lyase, polysaccharide structure analysis, alginate oligosaccharides (AOS) have many biological activities including act as prebiotics, immune modulator, anticoagulation, antioxidant, anticancer, growth promoting activities, promote production of antibiotics and ethanol. In relation to the importance of alginate lyases, their potential aplications and prospect in development of new bioactive products, we present review of the enzymes, sources, mechanism of activity and potential applications. This paper also discussed some new biological engineering in alginate lyase production.
Nutritional and Albumin Content of Swamp Fishes from Merauke, Papua, Indonesia Susilowati, Rini; Sugiyono, Sugiyono; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.268

Abstract

 Study on chemical content of swamp fishes from Merauke has been conducted to obtain nutritional status  of these fishes. Sampling was conducted  twice (August and November 2015) in Merauke. There were six dominant fish species, namely striped snakehead (Channa striata), nile tilapia (Oreochromis niloticus), tade gray mullet (Liza tade), philippine catfish (Clarias batrachus), barramundi (Lates calcarifer), and climbing perch (Anabas testudineus). Result on proximate analysis showed that the fishes had moisture content of 75.73±0.25%-81.45±0.04%, ash 0.94±0.01%-1.26±0.21%, protein 17.11±0.09%-18.92±0.05%, and lipid 0.59±0.19%-3.80±0.63%. The dominant essential amino acid of 6 swamp fishes from Merauke was lysine followed by leucine. Meanwhile the dominant non-essential amino acid was glutamic acid (32.26+0.61 mg/g), followed by aspartic acid. Calcium (Ca) was the dominant macro mineral for all fishes studied, being the highest was in nile tilapia (328.76±8.14 mg/100 g). Micro mineral was dominated by the presence of Selenium (Se) which the highest was in nile tilapia (0.084±0.005 mg/100g). These fishes were also rich in albumin, being the highest was stripe snakehead (138.59±1.68 mg/g). The average of total fatty acid showed that the fishes had saturated fatty acid (SFA) content of 46.30±0.27%, monosaturated fatty acid (MUFA) 9.20±1.6%  and polyunsaturate fatty acid (PUFA) 34.49±0.44% of total fatty acid. From the result, it can be concluded that fish harvested from swamp of Merauke and surrounding area had a good nutritional value.
Feasibility of metabolomic analysis in assessing coral reefs environments. Hedi Indra Januar; Boedi Hendrarto; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 2 (2010): August 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i2.45

Abstract

This study aimed to explain the feasibility of fingerprint and chemical marker metabolomicanalysis in assessment of coral reefs environments. Based on literature review, both analysismight be implemented on coral reef environment assessment, with their specific strength andweakness. Fingerprint metabolomic is a comprehensive approach in assessing the condition ofcoral reefs bioindicator that is having environmental stresses. It require high technology chemicalinstrumentation and complicated statistical approach in post data analysis. On the other hand,chemical marker metabolomic is more simplified in data analysis and instrumentation, but notcomprehensively assess the metabolite changes of bioindicator that is having environmentalstresses. To apply as high throughput screening in coral reefs assessment, subjectively the chemicalmarker method would be more feasible. One of the potential chemical marker that used iscembranoids, a bioactive compound from soft corals.
TEKNIK ISOLASI SENYAWA BIOAKTIF DARI KAPANG YANG BERASAL DARI LINGKUNGAN LAUT Noviendri, Dedi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 3, No 2 (2008): December 2008
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v3i2.161

Abstract

Pada prinsipnya, teknik isolasi senyawa bioaktif dari kapang yang berasal dari laut mirip dengan teknik isolasi senyawa bioaktif dari invetebrata laut dan juga biota-biota yang ada di lingkungan darat. Namun ada beberapa hal yang berbeda, diantaranya cara isolasi dan pengkulturan terutama pada komposisi media pertumbuhannya, serta cara-cara pemanenannya. Sedangkan cara ekstraksi, fraksinasi, dan purifikasinya sampai mendapatkan suatu senyawa bioaktif yang murni adalah relatif sama. Untuk proses pemisahan dan purifikasi dapat digunakan alat Kromatografi Cair Kinerja   Medium (KCKM) dan Kromatografi Cair Kinerja Tinggi (KCKT), serta disertai pengujian kemurnian dengan Kromatografi Lapis Tipis (KLT).  Jika dari hasil analisis dengan KCKM atau KCKT memberikan hasil satu puncak (peak) yang tunggal, maka terhadap sampel tersebut  dapat dilakukan analisis dengan alat MS, dan hasilnya dibandingkan dengan database untuk menguji apakah senyawa murni yang diperoleh adalah senyawa baru atau bukan. Selanjutnya dengan semua data analisis  yang diperoleh dari sampel murni tersebut, yaitu data fisika dan hasil spektrofotometer UV, hasil IR (KBr), MS, NMR 1 dimensi (proton, karbon, dan DEPT), dan NMR 2 dimensi (1H-1H COSY, 13C-1H COSY, HMBC, HMQC, dan NOESY), maka dilanjutkan dengan penentuan struktur molekul (dikenal dengan elusidasi struktur).
IDENTIFICATION OF VOLATILE FLAVOUR COMPOUNDS OF HOKI (Macruronus novaezelandiae) AND ORANGE ROUGHY (Hoplostethus atlanticus) OILS Hari Eko Irianto; Carmen C. Fernandez; G. J. Shaw
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 2 (2014): August 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i2.105

Abstract

Identification of volatile flavour compounds of hoki (Macruronus novaezelandiae) and orange roughy (Hoplostethus atlanticus) oils has been carried out.  Flavour compounds were extracted by a purging system and collected using a porous polymer Tenax TA trap.  The gas chromatography-mass spectrometry (GC-MS) was used to identify the volatile flavour compounds.  The predominant compounds contributing to the volatile flavour of hoki oil were methyl ethyl benzoate, ethyl benzoate and 1,1-dimethylethyl-2-propionic acid.  Meanwhile, the main volatile flavour components of orange roughy oil were toluene, cyclohexane, 1,1-dimethylethyl-2-methyl propionic acid and tetrachloroethane.  
Proximate Composition and Sensory Characteristics of Traditional and Oven-Drying Smoked Tilapia Fillets Enriched With Olive Oil Ikasari, Diah; Suryanti, Suryanti; Suryaningrum, Theresia Dwi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.291

Abstract

The study aimed to investigate the chemical and organoleptic characteristics of smoked tilapia fish fillets which were produced using different drying process and enriched with olive oil. Smoked fish were made using the following method: the fish were fasted overnight, shocked with cold water (4-5 oC) and then slaughtered and filleted. The fillets were soaked for 30 minutes in a brine solution containing spices and 5% (v/v) of liquid smoke. 5% (v/v) of olive oil was added into the solution and compared with control without olive oil addition. After 30 minutes, fish were cleaned from the spices using water and smoked at a temperature of 80 oC for 4-5 hours. The drying process was conducted in two different methods, i.e using oven-drying and traditional smoking. The smoked tilapia products were analyzed for proximate composition and sensory profiles. The result showed that addition of olive oil and methods of drying affected the proximate composition as well as sensory characteristics of the tilapia fillets. The fillets with addition of 5% olive oil and processed with oven-drying were considered as the best treatment due to low moisture content but high ash, fat and protein contents with 45.6±0.4%; 6.8±0.1%; 7.9±0.1% and 82±1.6%,  respectively. Based on panelist evaluation, the fillets with 5% olive oil and processed with oven-drying also had high performances in appearance and texture.         
Non-Target Screening Method for the Identification of Persistent and Emerging Organic Contaminants in Seafood and Sediment from Jakarta Bay Dwiyitno Dwiyitno; Larissa Dsikowitzky; Nuri Andarwulan; Hari Eko Irianto; Hanifah Nuryani Lioe; Farida Ariyani; Jan Schwarzbauer
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 3 (2015): December 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i3.175

Abstract

Identification of persistent and emerging organic contaminants in green mussels (Perna viridis), various fishes, banana shrimp (Penaeus merguiensis) and sediment samples from Jakarta Bay has been employed. A non-target GC-MS screening approach has identified more than 60 individual organic compounds from the whole fractions either non-polar, semi-polar or acidic-polar compounds. The substances comprised as persistent organic pollutants (POPs) including DDT (dichlorodiphenyl trichloroethane) and its metabolites as well as high molecular weight polycyclic aromatic hydrocarbons (HMW-PAHs). Noteworthy, a number of emerging contaminants detected in the present study have never been reported previously either from the same location or from Asian waters. They include some priority contaminants of non-persistence halogens and emission of technical products, such as di-iso-propylnaphthalenes (DIPNs) dichlorobenzene (DCB), dichlorodiphenyl chloroethene (DDMU) and phenylmethoxynaphthalene (PMN). In general, the concentration order of the priority organic contaminants was sediment green mussel fishes shrimp. Further analysis based on the spatial distribution, individual concentrations and bioavailability suggested that some contaminants are applicable as molecular marker for the assessment of anthropogenic emission in Jakarta Bay, i.e. DIPNs, linear alkylbenzenes (LABs), phenylmethoxynaphthalene (PMN), PAHs, dichlorobenzene, DDT and its metabolites.
Capturing Natural Product Biosynthetic Pathways from Uncultivated Symbiotic Bacteria of Marine Sponges Through Metagenome Mining: A Mini-Review Agustinus Robert Uria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.131

Abstract

Symbiotic bacteria associated with marine sponges have frequently been proposed as the true producer of many bioactive natural products with potent anticancer activities. However, the majority of these complex symbiotic bacteria cannot be cultivated under laboratory conditions, hampering efforts to access and develop their potent compounds for therapeutic applications. Metagenome mining is a powerful cultivation-independent tool that can be used to search for new natural product biosynthetic pathways from highly complex bacterial consortia. Some notable examples of natural products, in which their biosynthetic pathways have been cloned by metagenome mining are onnamide A, psymberin, polytheonamides, calyculin, and misakinolide A. Subsequent expression of the pathways in easily culturable bacteria, such as Escherichia coli, could lead to the sustainable production of rare promising natural products. This review discusses principles of metagenome mining developed to gain access to natural product biosynthetic pathways from uncultured symbiotic bacteria of marine sponges. This includes detecting biosynthetic genes in sponge metagenome, creating large metagenomic library, rapid screening of metagenomic library, and clone sequencing. For many natural products made by modular polyketide synthases (PKSs) and hybrids with non-ribosomal peptide synthetases (NRPSs), their biosynthetic pathways as well as structures of final products can be predicted with high accuracy through bioinformatic analysis and sometimes combined with functional proof. Further metagenome sequencing integrated with single-cell analysis and chemical studies could provide insights into the remarkable biosynthetic capacity of uncultivated bacterial symbionts, thereby facilitating the discovery and sustainable production of a wide diversity of sponge-derived complex compounds.
TEKNOLOGI DNA REKOMBINAN DAN APLIKASINYA DALAM EKSPLORASI MIKROBA LAUT Dedi Noviendri
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 2, No 2 (2007): December 2007
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v2i2.138

Abstract

Teknologi DNA rekombinan merupakan teknik penggabungan DNA dari spesies yang berbeda sehingga akan diperoleh organisme baru dengan sifat-sifat yang diinginkan. Pada pengembangan bioteknologi kelautan dan perikanan, teknik DNA rekombinan ini dapat digunakan antara lain untuk melakukan eksplorasi potensi dan biodiversitas organisme laut, seperti mikroba laut. Mikroba laut yang sebelumnya hanya merupakan kekayaan alam laut yang potensial dan belum memberikan nilai tambah, maka dengan teknologi DNA rekombinan dapat ditingkatkan nilai tambahnya untuk menghasilkan produk yang sangat prospektif. Secara garis besar, teknologi DNA rekombinan (rekayasa genetika) melibatkan penyisipan informasi genetik baru ke dalam organisme, biasanya bakteri, untuk memberikan kemampuan baru. Metode ini tidak mengikuti rangkaian prosedur yang pasti. Pemilihan metode bergantung kepada gen mana yang akan dipindahkan dan jenis organisme mana yang akan menerima informasi genetik baru. Pilihan tersebut bergantung pada sampai sejauh mana keterlibatan pilihan pribadi ilmuwan yang bersangkutan.

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