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Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
ISSN : 20895690     EISSN : 24069272     DOI : -
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 363 Documents
Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria Sedjati, Sri; Ambariyanto, Ambariyanto; Trianto, Agus; Supriyantini, Endang; Ridlo, Ali; Bahry, Muhammad Syaifudien; Wismayanti, Gita; Radjasa, ocky Karna; McCauley, Erin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.438

Abstract

This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.  Meanwhile, the  ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria
Bioactive Compounds, Cosmeceutical And Nutraceutical Applications of Green Seaweed Species (Chlorophyta) Muhammad Taher; Fatin Syazwani Ruslan; Deny Susanti; Normawaty Mohammad Noor; Nurul Iman Aminudin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 1 (2021): May 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.514

Abstract

Seaweeds are valuable marine plants that have garnered much attention from the public due to their high bioactive, nutrients and minerals content. Seaweeds have been used in multiple applications, including in cosmeceutical, nutraceutical and pharmaceutical industries. Nevertheless, this review will focus on the bioactive compounds of Chlorophyta and their potential application in nutraceutical and cosmeceutical industries. Chlorophyta are believed to possess a significant amount of nutrients and minerals, sufficient to meet the daily requirements of nutrients and minerals in the human body.  Considering the nutritional aspect, deficiency in nutrients may lead to severe ailments, including heart disease, neurological disorder and cancer. The main compounds studied in this review are polysaccharides, proteins, amino acids, lipids, fatty acids, pigments, minerals, vitamins and secondary metabolites. Among all, polysaccharides are the most exploited compounds and used in many advanced applications in the nutraceutical and cosmeceutical industries. This review also offers insights into the beneficial biological properties of Chlorophyta, highlighting their potential in cosmeceutical and nutraceutical applications. Further research is required to highlight the Chlorophyta sp. aquaculture, its extraction method, and the most targeted bioactive compounds from the species. Therefore, the challenge is to increase public awareness of the promising application of this species in the nutraceutical and cosmeceutical  industries.
Nutrient Composition, Antioxidant and Antibacterial Activities of Ulva prolifera O. F. Müller Arguelles, Eldrin De Los Reyes; B. Sapin, Arsenia
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Article in Press
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.550

Abstract

Seaweeds are marine organisms capable of producing diverse kinds of chemical compounds with promising pharmacological use. The study evaluated the proximate and elemental composition and the potential antioxidant (using CUPRAC, ABTS+, and DPPH assays) and antibacterial activities (using microtiter plate dilution assay) of Ulva prolifera O. F. Müller. The seaweed has a total phenolic content of 829 ± 2.00 mg GAE/g. Antioxidant efficiency of U. prolifera exerted high ability of reducing copper ions, potent ABTS+ and DPPH scavenging activities in a concentration-dependent manner with IC50 value of 24.7 mg GAE/mL, 43.52 mg GAE/mL, and 54.1 mg GAE/mL, respectively, more effective than ascorbic acid. In vitro antibacterial activity assay showed that U. prolifera exhibited inhibitory activity against bacterial pathogens such as Methicillin-resistant Staphylococcus aureus (MIC = 125 mg/mL), S. epidermidis (MIC = 125 mg/mL), and Pseudomonas fluorescens (MIC = 500 mg/mL). This investigation is the first documented report regarding antibacterial activity of U. prolifera against P. fluorescens. In addition, results showed elemental composition to be in decreasing order of Ca K Mg Na Mn Fe Zn Cu Pb Cd Cr. Proximate composition of U. prolifera showed high carbohydrate and protein content with a percentage composition of 36.20 ± 0.27% and 23.72 ± 0.31% (dry weight), respectively. This study is the first report in the Philippines that shows the potential of U. prolifera as an excellent candidate organism as source of chemical compounds with relevant application to the pharmacological industry.
Microencapsulation of Fucoxanthin by Water-in-Oil-in-Water (W/O/W) Double Emulsion Solvent Evaporation Method: A Review Noviendri, Dedi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 3 (2014): December 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i3.114

Abstract

Fucoxanthin is a major xanthophyll present in brown seaweeds such as Sargassum binderi, S. duplicatum, Turbinaria turbinata, Padina australis, Undaria pinnatifida and Hijkia fusiformis. This carotenoid has a unique structure including oxygenic functional group such as, two hydroxy, keto, epoxy (5,6-monoepoxide), and an allenic bond. Fucoxanthin has some anticancer activities such as, exhibits inhibitory property on colon cancer cells and human hepatic carcinoma HepG2 cell line. This xanthophyll also induces apoptosis of human leukemia cancer HL-60 cells, human prostate cancer PC-3 cell, human lung cancer H1299 cell line etc. Unfortunately, the poor solubility of this carotenoid in water hinders it to be a drug candidate. Fucoxanthin is also a pigment that is sensitive to temperature and light. One of the possible ways to circumvent the problem with light and temperature is by microencapsulating it. Microencapsulation (ME) in biodegradable polymers, e.g. poly(D,L-lactic-co-glycolic acid) (PLGA) is a promising approach to protect any potential drug from rapid degradation. Solvent evaporation method is the most popular technique of preparing PLGA microsphere (MS) and this technique has been extensively studied in recent years for the preparation of MS. In the water-in-oil-in-water (w/o/w) double emulsion solvent evaporation method, stability of the primary emulsion (PE) is a critical factor. When the PE is unstable, encapsulation efficiency (EE) is low. Stability of PE can be enhanced by including emulsifying agent or stabilizers such as polyvinyl alcohol (PVA). The presence of a stabilizer/ emulsifier plays a significant role in influencing particle size (PS), external morphology of microsphere and colloidal stability.  
Back Cover Squalen Bulletin Vol. 16 No. 3 Tahun 2021 bulletin squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 3 (2021): December 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Molecular Characterization of Caulerpa racemosa (Caulerpales, Chlorophyta) from Indonesia Based on the Plastid tufA Gene Muhamad Darmawan; Neviaty Putri Zamani; Hari Eko Irianto; Hawis H Madduppa
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 3 (2021): December 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.588

Abstract

The green seaweed Caulerpa racemosa is  a seaweed  of  high prospect that is being given emphasis by the Indonesian government. However, C. racemosa in Indonesia may include multiple species level-entity exhibiting morphological overlap and require molecular analysis for species identification. Molecular documentation of species richness of indigenous populations of C. racemosa is essential to underpin cultivar development and conservation of the species to avoid overharvesting. The present study aimed to determine the genetic diversity of C. racemosa and document the haplotype network of the specimens from four different locations (Bintan Island, Jepara, Takalar and Osi Island) using the chloroplast tufA gene. Twenty individuals from four areas were collected and amplified with the chloroplast-encoded gene tufA for species identification against publicly available data. The identification of C. racemosa based on the tufA gene showed that the species found in four locations were C. cylindracea (previously C. racemosa var. cylindracea), C. macra (previously C. racemosa var. macra), C. racemosa, and C. oligophylla (previously C. racemosa var. lamourouxii). This study records the existence of C. cylindracea in Takalar and Jepara, Indonesia for the first time. The most diverse C. racemosa species was in Osi Island, where the exploitation of this seaweed is very low. In contrast, the lowest number of C. racemosa varieties were found in Takalar, where exploitation is very high. There were only minor light variations of Caulerpa species in the tufA gene in four different sites with only four haplotypes found, and each haplotype corresponded to another species. 
Preface Squalen Bulletin Vol. 16 No. 2 Tahun 2021 squalen bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 16, No 2 (2021): August 2021
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v16i2.594

Abstract

Properties of Sodium Alginate–Polyvinyl Alcohol Hydrogels Irradiated By Gamma Ray For Wound Dressing Materials Thamrin Wikanta; Erizal Erizal
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): May 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i1.97

Abstract

A copolymer of sodium alginate (SA)–polyvinyl alcohol (PVA) was synthesized by gamma irradiation  to  make  a  hydrogel  and  study  on its properties has been carried out. A  series of 0.5% -2.0% (w/v) of SA solution and 10% (w/v) of PVA solution were prepared in separate tubes. Each of the SA solution was mixed with PVA solution, then it was irradiated with gamma ray at the doses of 30 kGy, 40 kGy, and 50 kGy (dose rate of 10 kGy/hour), respectively. The properties of hydrogels were evaluated comprising of the physical performance, gel fraction, swelling ratio, water evaporation, and pH. The results showed that increase of irradiation dose can increase the gel fraction, but  increase of SA concentration can decrease the gel fraction. SA concentration of 1-2% with gamma ray irradiation dose of 30 kGy was a suitable condition to produce a good hydrogel with properties as follow, gel fraction of 75.83%-78.70%, swelling ratio of 69.12-80.14 g/g, and maximum water evaporated of 54.21-76.41%, the pH value ranged 5.60-5.65. The hydrogels produced are suitable to be used for  wound dressing, especially for wet wounds, and can be applied for two days.
Peran Mutu Dalam Menunjang Ekspor Udang Nasional Sumpeno Putro
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 3, No 1 (2008): June 2008
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v3i1.164

Abstract

Udang merupakan  komoditi  andalan  ekspor  dari  sektor  perikanan  Indonesia. Akan tetapi, volume ekspor udang  Indonesia dalam  beberapa  tahun  terakhir  ini  cenderung menurun. Hal  ini terutama disebabkan karena semakin ketatnya persyaratan impor yang diberlakukan oleh negara-negara maju,  khususnya  standar mutu  dan  sanitasi. Ekspor udang  ke Uni Eropa merosot dan dikenakan RAS  (Rapid Alert System)  karena dicurigai mengandung  residu  antibiotik  dan bakteri patogen. Ekspor udang ke Amerika Serikat, juga masih dikenakan automatic detention. Sementara itu, pada  tahun 2006 dan  2007  ekspor udang  ke  Jepang  dan Cina  juga ditolak  karena  dicurigai mengandung  residu  antibiotik. Oleh  karena  itu,  pengembangan  sistem  pembinaan mutu  dan sertifikasi secara terpadu dengan konsep from farm to table sangat diperlukan untuk meningkatkan daya saing dan akses pasar dalam menghadapi era globalisasi. Dalam kaitan  ini, pengembangan industri  udang budidaya  ke depan  sebaiknya  tidak  hanya berorientasi  kepada upaya peningkatan produksi,  tetapi  juga memperhatikan  aspek mutu  dan  keamanan  pangan  (food safety)  agar produk  yang  dihasilkan  layak  dan  aman  untuk  dikonsumsi  serta memenuhi  standar mutu  yang berlaku  di  pasar  internasional.
PROSPEK PEMANFAATAN LIMBAH PERIKANAN SEBAGAI SUMBER KOLAGEN Nurhayati Nurhayati; Rosmawaty Peranginangin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 3 (2009): December 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i3.157

Abstract

Pengolahan hasil perikanan seperti pengalengan dan filet ikan biasanya memiliki produk samping berupa kepala, sirip, tulang, sisik maupun kulit ikan dalam jumlah yang melimpah. Limbah perikanan tersebut dapat diproses lebih lanjut menjadi kolagen yang bernilai ekonomis tinggi. Kolagen terdapat pada jaringan ikat dalam kulit, tendon, tulang keras, dan tulang rawan. Pelarutan protein non kolagen, mineral maupun lemak diperlukan untuk memudahkan proses ekstraksi kolagen. Umumnya proses ekstraksi kolagen dilakukan dalam suasana asam. Namun, penggunaan pepsin pada proses ekstraksi ternyata diketahui dapat meningkatkan rendemen kolagen yang diperoleh. Karakteristik kolagen dapat dilihat dari kelarutan, viskositas, suhu denaturasi, dan kandungan asam amino yang diperoleh. Saat ini, penggunaan kolagen pada industri telah meluas, khususnya pada bidang medis, pangan, dan kosmetik. Menurut data statistik, diperkirakan bahwa limbah perikanan sebesar 1,81 juta ton pertahun dihasilkan oleh perikanan tangkap. Jika rendemen kolagen berkisar antara 11–63% maka diperkirakan dapat dihasilkan kolagen dalam jumlah yang besar, yaitu sekitar 0,20–1,14 juta ton. Hal ini menunjukkan bahwa limbah perikanan dapat menjadi sumber kolagen yang potensial.

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