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Jurnal Biologi Tropis
Published by Universitas Mataram
ISSN : 14119587     EISSN : 25497863     DOI : -
Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi Sains.
Arjuna Subject : -
Articles 2,520 Documents
Phytochemical Profiling of Tampoi (Baccaurea macrocarpa (Miq.) Mull. Arg.) Leaves Using Liquid Chromatography-Tandem Mass Spectrometry Gaol, Agnes Della Lumban; Sujarwati
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.8964

Abstract

Tampoi leaves (Baccaurea macrocarpa (Miq.) Mull. Arg.) have been reported to contain a diverse array of phytochemicals associated with various biological activities, including antioxidant and antibacterial effects, as well as the enhancement of erythrocyte and hemoglobin levels in anemic mouse models. This study aimed to comprehensively characterize the phytochemical profile of tampoi leaves to establish a foundation for elucidating the mechanisms underlying their biological activities. Extraction was conducted using a solid phase method with methanol as the solvent. Phytochemical profiling was performed using Liquid Chromatography Tandem Mass Spectrometry (LCMS/MS) in positive ionization mode, and data analysis was carried out using MassLynx software. The analysis revealed the presence of 39 phytochemical compounds in the methanolic extract of tampoi leaves. These compounds were classified into 12 major groups: coumarin, amino acid, glycosides, flavonoids, inositol, tannin, terpenoids, jasmonic acids, alkaloids, saponin, phenols, and fatty acids. Among these, terpenoids constituted the most abundant class, comprising nine compounds. Vitexin and linolenic acid exhibited the highest chromatographic peaks, indicating relatively high abundances compared to other constituents in the extract. The phytochemical profile of tampoi leaves offers a valuable basis for understanding the interactions between bioactive compounds and biological targets through in silico approaches, thereby supporting the development of phytopharmaceutical candidates.
Growth and Yield of Cowpea Microgreens (Vigna unguiculata L. Walp) under Different LED Light Intensities and Growing Media Wiliem, Steven De; Zumani, Darul
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9062

Abstract

Cowpea microgreens (Vigna unguiculata L. Walp) are nutrient-rich foods with high nutritional content. The objective of this research was to ascertain how growing medium and 6500K white LED light intensity affected the development and production of cowpea microgreens. This investigation was conducted in January and February of 2025 in Nagarawangi Village, Cihideung Subdistrict, Tasikmalaya City, at a height of 355 meters above sea level.  Using a factorial randomised block design, the research used an experimental approach with two components: two kinds of growth medium (m1 (rockwool) and m2 (vermicompost)) and five degrees of light intensity (i0 (control), i1 (3000 lux), i2 (4000 lux), i3 (5000 lux), and i4 (6000 lux).  Every therapy was carried out three times.   The observational data were analyzed at a significance level of 5% using the Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT).  The findings indicated that fresh weight and germination speed parameters were influenced by both growth material and light intensity.  Microgreens' height and germination rate were influenced by light intensity and growth medium separately. Based on the results, the treatment of 5000 lux light intensity with rockwool growing media produced the best growth and yield of cowpea microgreens (Vigna unguiculata L. Walp).
Primer Construction of Transporter Genes Based on Almt1 for The Analysis of Cmp-Sialic Acid Transporter Gene in Derendan Taufikurrahman, Taufikurrahman; Putri, Fatma Jumaita; Nurbaiti, Nurbaiti; Ramadhani, Putri Oktavia; Wahibah, Ninik Nihayatul
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9069

Abstract

Derendan (Lansium sp.) is an underutilized endemic fruit tree species from Bengkalis Island that grows in acidic peatland soils with high concentrations of toxic aluminum ions (Al³⁺). This study aimed to construct gene-specific primers to detect stress tolerance genes in derendan, with an initial focus on the ALMT1 (Aluminum-activated malate transporter) gene, which is known to mediate aluminum detoxification through organic acid exudation. Primers were designed based on ALMT1 sequences from model species and tested using PCR amplification and sequencing of derendan genomic DNA. Surprisingly, the amplified DNA fragment showed high similarity with CMP-sialic acid transporter 1 genes in several plant species, based on BLASTn analysis. The CMP-sialic acid transporter gene encodes a protein involved in the transport of sialic acid, an organic acid known to play roles in abiotic stress responses. These findings suggest that the constructed primers may target conserved transporter domains related to stress adaptation. The resulting primers have potential applications in molecular studies and breeding programs aimed at enhancing peatland stress tolerance in derendan and related species.
The Effect of Basil Essential Oil Addition on the Functional Properties of Gelatin-Based Edible Film Ayu Lestari, Dwi; Fahrullah, Fahrullah
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9123

Abstract

Edible film is a thin film that can be consumed immediately and can be used as a food packaging material. The incorporation of basil essential oil is anticipated to enhance the functional characteristics of edible film. The objective of this study is to evaluate the impact of incorporating basil essential oil on the water vapor transmission rate, gelation time, and the chemical structure of gelatin-based edible films. The research method employed a completely randomized design, incorporating three distinct treatments and three replications to ensure the robustness of the findings. The treatments comprised K1, which did not include the addition of basil essential oil; K2, which incorporated 4% basil essential oil; and K3, which incorporated 8% basil essential oil. The results demonstrated that the incorporation of basil essential oil led to a significant (P<0.05) increase in the WVTR value, with elevated concentrations resulting in increased WVTR. However, the incorporation of basil essential oil did not demonstrate a statistically significant impact on the gelation duration (P>0.05). FTIR analysis revealed an interaction between essential oil and gelatin that affected the film structure, thereby increasing the flexibility and antibacterial potential of the film. In conclusion, the incorporation of basil essential oil has been demonstrated to enhance the functional properties of gelatin-based edible films.
Biomagnification of Mercury Through the Aquatic Food Chain in Lake Lebo Taliwang West Sumbawa Rahman, Abdul; Maria Ulfa, Andi; Junaidi efendi; Supriastuti, Eko
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9452

Abstract

Lebo Taliwang Lake has been reported to be polluted by mercury compounds. This study aims to determine the occurrence of mercury biomagnification in the food chain in Lebo Lake Taliwang. Samples of water, tilapia, water strider and japan pond heron were taken at two sampling locations: inlet and outlet. Samples were tested for mercury content with a mercury analyzer. The results show that there is mercury accumulation in the Lebo Taliwang Lake ecosystem. The average of each sample was 1.95 in water, 99.5 in tilapia, 79.8 in water srider, and 948.09 and 143 in feathers and blood, respectively. Furthermore, the results of the biomagnification factor (BMF) value show the BMF>1. This means that there is biomagnification of mercury in Lebo Taliwang Lake. The biomagnification value of japan pond heron in feather and blood with nile tilapia is 9.52 and 1.43. Meanwhile, with water stider, the values are 11.87 and 1.81. This study recommends enhanced monitoring and regulation of illegal gold mining (PETI) activities, as well as environmental rehabilitation efforts around Lake Lebo
The Potential of Gelam (Melaleuca leucadendra) Bark Extract as an Antibacterial Agent Against Pseudomonas aeruginosa and Enterococcus faecalis Yusro, Fathul; Mariani, Yeni
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9012

Abstract

This study aims to evaluate the antibacterial activity of Melaleuca leucadendra (Gelam) bark extract against Pseudomonas aeruginosa and Enterococcus faecalis. Bark samples were collected from Karimunting Village, Bengkayang Regency, and extracted using maceration with 96% ethanol. Antibacterial testing was conducted using the disc diffusion method on MHA (Mueller Hinton Agar), with bacterial suspensions adjusted to McFarland standard 1. The results showed that the extract exhibited concentration-dependent antibacterial activity. The highest inhibition zones were observed at a concentration of 200 mg/mL, measuring 4.5 mm for P. aeruginosa and 3.5 mm for E. faecalis. Although the inhibitory effect was relatively low compared to the positive control (amoksilin), these findings indicate the potential of Gelam bark extract as a natural antibacterial agent. Further studies are recommended to isolate active compounds, assess toxicity, and develop formulations to enhance its antibacterial efficacy.
Identification of Family Members Gobiidae in the Mangrove Forest Nature Reserve on the East Coast of Jambi Province Rosalia, Ipana; Nugraha, Anggit Prima; Setiyaningrum, Dewi Aswin; Uljannah, Lulu’
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9031

Abstract

Jambi Province has abundant natural resources, including mangrove forests that serve as habitats for various animal species. This study aimed to increase understanding of conservation and to identify types of mudskippers and the characteristics of their habitats in the East Coast Mangrove Forest Reserve. The research used purposive sampling at three different spots: residential areas, rivers, and mangrove zones. The study found two fish species (Boleophthalmus boddarti and Periophthalmodon schlosseri) each found in different areas depending on their preferred habitats. In conclusion, each type of habitat supports the growth of mudskippers, and it's recommended that the mangrove area’s sustainability continues to be protected.
Effect of Lemongrass Essential Oil on Gelatin Edible Film: Water Vapor Transmission, Gelation, and Chemical Structure Fahrullah, Fahrullah; Rosida, Abidatur
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9124

Abstract

Edible film constitutes a thin layer that is capable of biodegradation and is therefore considered safe for consumption, thus providing a more environmentally friendly alternative to conventional plastics. The objective of the present study was to ascertain the impact of lemongrass essential oil incorporation on the water vapour transmission rate, gelation time and chemical structure of gelatin edible film. The present study employed a randomised experiment, incorporating 3 distinct treatments and 3 replicates for each treatment. The treatments comprised three groups: P1, which did not include the addition of lemongrass essential oil; P2, which incorporated the addition of lemongrass essential oil at a rate of 4%; and P3, which incorporated the addition of lemongrass essential oil at a rate of 8%. The findings demonstrated that the incorporation of lemongrass essential oil did not exert a substantial influence on the WVTR value (P > 0.05), with the mean value remaining within the parameters stipulated by the Japanese Industrial Standard. Conversely, the gelation time exhibited a significant increase (P<0.01) with rising lemongrass oil concentrations, attributable to the disruption of gelatin intermolecular bonds. FTIR analysis demonstrated interactions between the film components, with an increase in the intensity of certain functional groups indicating alterations in the chemical structure, particularly at a concentration of 4%, which resulted in optimal interactions without compromising the gelatin protein structure. The study concluded that the incorporation of lemongrass oil at concentrations up to 4% yielded optimal outcomes in enhancing the flexibility and antimicrobial properties of edible film, without compromising its structural stability.
Identification of Neisseria gonorrhoeae in Commercial Sex Worker Clients with and without Condom Use Maulandi, Andi; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9142

Abstract

This study investigates the prevalence of Neisseria gonorrhoeae among commercial sex workers and their clients in Pulau Baai, Bengkulu, with a focus on comparing infection rates between individuals who use condoms and those who do not. The primary objective is to assess the impact of condom usage on the prevalence of gonorrhea in this high-risk population. A cross-sectional observational design was employed, involving 40 male participants who had engaged with commercial sex workers in the Pulau Baai area. The participants were divided into two groups based on their condom use. Urethral secretion samples were collected and analyzed via Gram staining for Neisseria gonorrhoeae detection. The study found no cases of Neisseria gonorrhoeae in any of the samples, with all 36 condom users and 4 non-users testing negative. These results suggest no gonorrhea infections in the studied population, regardless of condom use. However, due to the limitations of the Gram staining method, further research using more sensitive diagnostic techniques and a larger sample size is recommended to verify these findings.
Identification of Neisseria Gonorrhoeae in Female Sexual Workers (FSW) Localized on Baai Island, Bengkulu City Lenzu, Henrek Miko; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9152

Abstract

Sexually Transmitted Infections (STIs) pose a significant global health challenge, particularly among high-risk populations such as female sex workers (FSWs). Neisseria gonorrhoeae, a causative agent of gonorrhea, is a major concern due to its prevalence and transmission via sexual contact. This study aims to identify the presence of Neisseria gonorrhoeae in female sex workers (FSWs) at the Pulau Baai locality, Bengkulu City, and examine the correlation between infection rates and factors such as duration of work and frequency of client visits. A descriptive observational study with a cross-sectional design was conducted, involving 31 FSWs who participated in the study after providing informed consent. Samples were collected through vaginal swabs and examined using Gram staining to identify Neisseria gonorrhoeae. The findings revealed that 3.2% (1 out of 31) of the participants tested positive for Neisseria gonorrhoeae. Statistical analysis showed that the risk of infection increased with the duration of work, as well as the frequency of client visits. The study concludes that while the overall infection rate is low, the prevalence of Neisseria gonorrhoeae remains a concern in this high-risk group. Regular screenings and preventive measures, including consistent condom use, are recommended to reduce transmission among FSWs.

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