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Agrin : Jurnal Penelitian Pertanian
Published by Universitas Jenderal Soedirman
ISSN : 14100029     EISSN : 25496786     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Agrin provides facilities for publishing articles or quality papers in the form of research results in various aspects of agriculture and agricultural commodities widely including ; agronomy, agroecology, plant breeding, horticulture, soil science, plant protection, agribusiness, agroforestry, food science and technology , agricultural techniques, agricultural innovations, agricultural models and agricultural biotechnology. This journal is published twice a year, ie the April and October. The Agrin Journal invites researchers, academics and intellectuals to contribute critical writing and contribute to the development of agricultural science.
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VIRULENSI 9 ISOLAT FUSARIUM OXYSPORUM F.SP. LYCOPERSICI DAN PERKEMBANGAN GEJALA LAYU FUSARIUM PADA DUA VARIETAS TOMAT DI RUMAH KACA Ambar, Abdul Azis; Priyatmojo, Achmadi; Hadisutrisno, Bambang; Pusposendjojo, Nursamsi
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.104

Abstract

Penyakit layu fusarium disebabkan oleh Fusarium oxysporum f.sp. lycopersici (Fol), merupakan penyakitpenting tomat. Penelitian ini bertujuan mengetahui tingkat virulensi Fusarium oxysporum f.sp. lycopersici darilokasi berbeda pada varietas Roma dan Money Maker, dan perkembangan penyakit layu fusarium di rumahkaca. Penelitian ini menggunakan suspensi Fol hasil monospora (1 x 107 konidium/ml air steril) dan tomat yangberumur 4 minggu. Akar direndam dalam suspensi Fol selama 30 menit, kemudian di tanam dalam pot yangtelah berisi campuran tanah dan pupuk. Hasil uji virulensi Fol isolat (A1) dan (A2) memperlihatkan virulensitinggi pada 2 varietas tomat yaitu (78%; 75%) untuk Roma dan (92%; 85%) untuk Money Maker. Keduaperlakuan tersebut berbeda nyata dengan kontrol. Rata-rata virulensi isolat Fol pada varietas Roma lebih rendah(< 50%) dibanding varietas Money Maker (> 50%). Hasil ini mengindikasikan bahwa varietas Roma lebih tahandibanding varietas Money Maker. Hasil pengamatan untuk perkembangan gejala layu fusarium di rumah kacaberupa menguningnya kotiledon kemudian kotiledon layu, diikuti mengeringnya ujung daun pertama. Gejalaberlanjut, mengering sampai gugurnya kotiledon yang diiringi dengan awal menguningnya daun, semua ujungdaun kering dan daun menguning, dan akhirnya daun layu. Gejala lain tampak dari penelitian adalah pengerdilantanaman pada kedua varietas.Kata kunci: virulensi fol, perkembangan gejala layu, varietas tomat ABSTRACTFusarium wilt diseases caused by Fusarium oxysporum f.sp. lycopersici (Fol) as important diseases ontomato. The aim of research was to know the levef of Fol virulence from 9 different origin (Centra Java, SouthSulawesi, and DI. Yogyakarta) and development of fusarium wilt diseases in green house. Monosporic colonysuspension (1x 107 conidia/ml) are used in this research. The test of virulence by pouring the monosporicsuspension of Fol isolates for 30 minutes on both varieties Roma and Money Maker were 4 weeks old. The resultshowed that A1 and A2 isolates more severity (78% and75% on Roma variety) and (92% and 85% on MoneyMaker variety) than other isolates. Amount of virulence showed Roma variety (< 50%) lower than MoneyMaker variety (> 50%). This result indicated that Roma variety more resistant than Money Maker variety. Theobservation of fusarium wilt development showed that early symptom of yellow – wilt cotyledont followed withdry of leaf tip. By the time, development of symptom was cotyledon senesence to followed yellow of leaf and theend wilt. The other symptom was inhibition of plant growth on tomato.Key words: fol virulency, development of wilt symptom, tomato variety
KERAGAAN PENDAPATAN PETANI LAHAN KERING DI KECAMATAN TARAKAN TIMUR KOTA TARAKAN MS, Etty Wahyuni
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.109

Abstract

Tujuan penelitian ini adalah untuk mengidentifikasi keragaan Pendapatan Petani di lahan keringberdasarkan umur petani, tingkat pendidikan, luas lahan, jumlah tanggungan, usahatani dan non usahatani.Penelitian telah dilaksanakan pada petani lahan kering di Mamburungan Timur dan Kampung Enam pada bulanOktober sampai Desember 2008, dengan penelitian kombinasi antara penelitian menerangkan dan penelitiandeskriptif. Hasil penelitian menunjukkan bahwa umur rata-rata petani secara umum adalah 48-53 tahun,menunjukkan bahwa petani dalam usia produktif, tingkat pendidikan secara umum adalah SMP (42,5%)menunjukkan kondisi yang cukup memadai untuk menerima serta menerapkan paket teknologi, luas lahanberagam dari dari 1-5 ha tetapi belum diusahakan maksimal oleh petani, jumlah tanggunganper kk petani antara4-5 orang-orang, sumber pendapatan petani masih bertumpu pada sektor pertanian khususnya komoditashortikultura sayur-sayuran dan buah-buahan dan campuran antara hortikultura dan tanaman pangan( 72,5%)hanya 27,5% menjawab memiliki usaha lain diluar sektor pertanian, sedangkan tingkat pendapatan petaniberkisar dari Rp. 750.000-Rp. 1.000.000 per bulan. Analisis model menggunakan metode Chi-Squaremenunjukkan bahwa umur, luas lahan dan sumber pendapatan memiliki hubungan dengan tingkat pendapatanpetani, namun tingkat pendidikan tidak memiliki hubungan terhadap tingkat pendapatan petani.Kata kunci: pendapatan petani,karakteristik petani, pertanian lahan kering ABSTRACTThis research aimed to identify the Income Performance of Farmers Dryland pursuant to characteristic offarmer cover age, mount education, wide farm, sum up responsibility, source of earnings and mount earnings.This research have been done for farmers dryland in chief of village Mamburungan and Kampung Enam onOctober until December 2008, with combination between explanatory research and descriptive research. Resultof research indicate that age of farmer in general range from 48-53 year representing productive age, mounteducation in general SMP (42,5%) representing condition which adequate enough to accept and also applytechnological packet fomentation, wide farm range from 1-5 ha but not yet maximal laboured by farmer, whileamount of responsibility per kk of farmer of responder between 4-5 people. Source of Earnings of farmer still beconvergent effort agriculture chosenly commodity of horticulture of vegetables and fruits or joined to plant foodcrop (72,5%) only 27,5% responder owning the effort peripheral outside agricultural activity, while storey ;level of earnings of farmer of responder range from Rp. 750.000-Rp. 1.000.000 per month. The model foranalysis was that of Chi-Square method show there are relation between age, wide farm and source of earningsto storey ; level of earnings of responder farmer, but mount education not own relation with storey; level ofearnings of responder farmer.Keyword : mount earnings, farmer characteristic, dry farming
ANALISIS PEMASARAN MANGGA “GEDONG GINCU” (Studi kasus di Kabupaten Cirebon, Jawa Barat) Supriatna, Ade
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.105

Abstract

Mangga Gedong Gincu mempunyai prospek baik untuk dikembangkan karena mempunyai karakteristik yangsesuai dengan permintaan pasar, yaitu mempunyai kulit mangga berwarna merah, komponen serat pada daging buahcukup banyak dan mempunyai aroma sangat tajam. Penelitian ini dilaksanakan tahun 2006 di Kabupaten Cirebon,sebagai sentra produksi mangga di Jawa Barat. Tujuan penelitian, yaitu: (i) menggambarkan rantai tataniaga mangga(ii) mempelajari karakteristik pelaku lembaga pemasaran dan (iii) menganalisis margin tataniaga. Penelitianmenggunakan metoda survai. Dimana data primer dikumpulkan dari 50 petani yang diambil secara acak (RandomSampling) dan pelaku lembaga pemasaran dengan metode snowball sampling. Data sekunder diperoleh dari DinasPertanian, Badan Pusat Statistik dan Lembaga Penelitian. Hasil penelitian menginformasikan, bahwa buah GedongGincu dipasarkan dalam bentuk grade A/B dan grade C (non grade). Pemasaran grade A/B melalui dua saluran,yaitu, pertama: petani – pengumpul - pedagang besar – agen - toko/kios buah – konsumen dan kedua: petani –pengumpul – pedagang besar – agen – suplayer – supermarket – konsumen sedangkan grade C melalui satu saluran,yaitu saluran ketiga: petani – pengumpul – pedagang besar – pedagang pasar tradisional – konsumen. Marjinpemasaran saluran pertama Rp.10.920,-/kg, berasal dari pedagang besar (48,1%), toko/kios (35,4%), agen (14,2%)dan pengumpul (2,3%). Marjin pemasaran saluran kedua Rp.15.000,-/kg, berasal dari pedagang besar (34,9%),suplayer (26,6%), supermarket (26,6%), agen (10,2%) dan pengumpul (1,7%). Keuntungan pedagang besar lebihtinggi dibandingkan agen, yaitu masing-masing Rp.3.350,-/kg dan Rp.1.460,-/kg. Permasalahan pemasaran manggaGedong Gincu yaitu posisi petani seringkali lemah dalam penentuan harga jual, jumlah serta mutu produk yangdihasilkan tidak selalu sesuai permintaan pasar, petani bermodal lemah sering terperangkap ke pelepas uang (moneylender) dan ditemukan pungutan-pungutan liar dalam kegiatan transportasi pengiriman mangga ke agen di pasar-pasarinduk. Dalam hal ini, perlu peningkatan aksesibilitas petani terhadap informasi pasar termasuk permintaan, variasi hargamusiman dan trend harga dengan demikian mereka dapat menyesuaikan rencana penjualan mangga untuk mencapaipenjualan efisien dan menguntungkan.Kata kunci: mangga, analisis pemasaran, Jawa Barat ABSTRACTGedong Gincu have a good prospect to be developed because it has some characteristics as good as marketrequest, namely mango’s skin is red, mango contains a lot of fiber components and aroma of ripe mango is verysharply This study was conducted in 2006 and took place in Cirebon District, as centre of mango production of WestJava. The objectives of study were; (i) to describe the marketing channel, (ii) to learn the characteristics ofmarketing institution and (iii) to analyze marketing margin of mango. This research used method of survey applyingstructured questionnaires. Primary data were collected from 50 farmers selected by random sampling and somemarketing institutions using snowball method. Secondary data were collected from the Agriculture Office, the CentreAgency of Statistic and the Research Institutions. Results showed, that mango was marketed in the form of grade A/Band grade C (non grade). Grade A/B had two marketing channels, namely; (i) farmers – collector – wholesaler –agent – fruit shop – consumer and (ii) farmers – collector – wholesaler – agent – supplier – supermarket –consumer. While grade C had one channel, namely (iii) farmers – collector – wholesaler – nonstore retailer –consumer. The first channel got marketing margin of Rp.10,920,- coming from wholesaler (48.1%), fruit shop(35.4%), agent (14.2%) and collector (2.3%). The second channel got marketing margin of Rp.15,000,- comingfrom wholesaler (34.9%), supplier (26.6%), supermarket (26.6%), agent (10.2%) and collector (1.7%). Theproblems in marketing were the farmers have a low bargaining position in determining the mango’s price, theamount and quality of product did not always accord to market request, the farmers that have weak capital wereoften fallen on money lender and there were found the illegal charges in activity of transportation. Somerecommended efforts in farmer’s level, namely to increase the farmer’s knowledge and skill to produce mango’squality according to market request by low cost technology, to improve the farmer’s accessibility to marketinformation inclusive of request, variation of seasonal price and trend price thereby they can plan the mango sale toreach effective, efficient and profit sale.Key words: mang, marketing analysis, West Java
KERAGAAN FENOTIP DAN BEBERAPA PARAMETER GENETIK HASIL DAN KARAKTER AGRONOMI ENAM PADI HIBRIDA DI LAHAN KERING MASAM Bambang Sutaryo; Tri Sudaryono
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.106

Abstract

Tujuan penelitian adalah untuk mengevaluasi keragaan parameter fenotip dan genetik hasil serta karakter agronomi dari enam padi hibrida di lahan masam Jasinga, Jawa Barat, selama musim kemarau (MK), dari bulan April hingga Agustus 2008. Enam padi hibrida yaitu IR58025A/Kapuas, IR68885A/Kapoas, IR58025A/Mendawak, IR68885A/Batanghari, IR58025A/Inderagiri, IRS 8025A/Lambur dan varietas pembanding lokal Hawara Bunar dirancang menggunakan Rancangan Acak Kelompok dengan tiga ulangan. Hasil penelitian menunjukkan bahwa hasil gabah empat padi hibrida yaitu IRS8025A/Kapuas (6,8 t/ha), IR68885A/Kapuas (6,6 t/ha), IRS 8025A/Mendawak (6,6 t/ha) dan IR68885A/Batanghari (6,4 t/ha) lebih tinggi dibandingkan dengan varietas kontrol Hawara Bunar (4,9 t/ha). Nilai variabilitas genetik yang luas terdapat pada hasil gabah (0,12), umur tanaman (16,34), tinggi tanaman (20,48), jumlah anakan produktif per rumpun (5,14), dan jumlah gabah isi per malai (41,37). Nilai duga heritabilitas tinggi terdapat pada hasil gabah (0,92), umur tanaman (0,68), tinggi tanaman (0,63), jumlah anakan produktif (0,95) dan jumlah gabah isi per malai (0,95). Korelasi genetik dan korelasi fenotip positif nyata antara hasil gabah dengan jumlah gabah isi per malai (0,05*), umur tanaman (0,25*), tinggi tanaman (0,12*), dan jumlah anakan produktif per rumpun (0,07*). Kata kunci : keragaan fenotip, genetik, agronomi, padi hibrida, lahan kering masam ABSTRACT The objective of the experiment was to evaluate phenotypic performance and genetic parameters of yield and agronomic traits of six hybrids rice at acidic dryland in Jasinga, West Java, during dry season of April-August 2008. Six hybrids rice such as IR58025A/Kapuas, IR68885A/Kapuas, IRS 8025 A/Mendawak, IR68885A/Batanghari, IR58025A/Inderagiri, IRS8025A/Lambur and Hawara Bunar (control) were designed by using Randomized Complete Block Design in three replications. The results showed that yield of four hybrids rice namely IRS8025A/Kapuas (6.81 ha"1), IR68885A/Kapuas (6.61 ha"1 ), IRS8025A/Mendawak (6.6 t ha"1) and IR68885A/Batanghari (6.4 t ha"1) were higher than that of Hawara Bunar (4.9 t ha"1). Broad genetic variabilities were found for grain yield (0.12), plant maturity (16.34), plant height (20.48), number of productive tiller per hill (5.14), and filled grain per panicle (41.37). High heritability estimate was found for grain yield (0.92), plant maturity (0.68), plant height (0.63), number of productive tiller per hill (0.95), and filled grain per panicle (0.95). Genetic and phenotypic correlation was found positive and significant between grain yield and filled grain per panicle (0.05*), plant maturity (0,25*), plant height (0.12*), and productive tiller per hill (0.07*). Key words: phenotypic, genetic, agronomic traits, hybrid rice, acidic dryland
UJI PENDAHULUAN KLON-KLON BAWANG MERAH ( Allium ascollonicum L.) First screening at shallot clones (Allium ascollonicum L.) Putrasamedja, Sartono
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.111

Abstract

UJI PENDAHULUAN KLON-KLON BAWANG MERAH ( Allium ascollonicum L.)First screening at shallot clones (Allium ascollonicum L.) ABSTRACT.Research was canducted same information candidat superior some clone from hibriditation theexperimental, experiment was carried out at experimental garden of districk farmer Kramat-Tegal, Central Java(6 m masl) from April to June 2007. The experiment was arranged. Randomised Block Design (R.B.D) with thosereplication. Clones to experiment contain 13 treatmen, clone 2, clone, clone3, clone5, clone6, clone7, clone11,clone12, clone21,clone24, local cultivity of Bauji, Bima Brebes and Phillipina to control. The result that clone 4to hights potential of product 18,446 ton/Ha, clone 7, clone 11 and clone 21 haved average of bulp diameter2,5cm and clone 3 haves 6 of sprout.Key words: cultivar, first of screening, clone, hibriditation.
KIAT USAHA PENGRAJIN TEMPE DAN BURUH PEREMPUANNYA AKIBAT KENAIKAN HARGA KEDELAI DI KECAMATAN KEMBARAN KABUPATEN BANYUMAS W, Djaniah; N., Dyah Ethika
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.107

Abstract

Penelitian ini bertujuan untuk mengetahui: 1). Usaha yang dilakukan pengrajin tempe dan buruhperempuannya akibat kenaikan harga kedelai di Desa Pliken Kecamatan Kembaran. 2). Tingkat penyerapantenaga kerja buruh perempuan dan upah yang diterima pada pengrajin tempe di Desa Pliken kecamatanKembaran. 3).Harga pokok produksi per unit industri tempe dan harga faktor produksi yang digunakan di DesaPliken Kecamatan Kembaran akibat kenaikan harga kedelai. 4). Biaya dan pendapatan usaha industri tempe diDesa Pliken Kecamatan Kembaran akibat kenaikan harga kedelai. Metode dasar dalam penelitian ini adalahmetode survai. Penelitian dilakukan di Desa Pliken Kecamatan Kembaran, Kabupaten Banyumas. Unit samplingyang diambil adalah pengrajin tempe dan buruh wanitanya. Pengambilan sampel pengrajin tempe dilakukandengan metode Simple Random Sampling diperoleh jumlah sampel sebanyak 31 pengrajin tempe. Hasilpenelitian menunjukan bahwa akibat kenaikan harga kedelai pengrajin tetap menjual tempe dengan harga yangsama tetapi ukuran tempe diperkecil atau mengurangi isi per bungkus; sebagian pengrajin menggunakancampuran singkong; mengurangi jumlah jam tenaga kerja luar keluarga; menaikkan harga jual tempe. 70 persenburuh wanita masih bekerja sebagai pembungkus tempe dan 30 persen menambah pekerjaan lain sebagai buruhtani atau berdagang. Upah tenaga kerja dihitung dengan satuan HOK, rata-rata kerja 4 jam/hari. Buruh wanitayang bekerja pada tiap pengrajin rata rata 3 orang, denga memperoleh upah rata-rata Rp6000,00 /hari. Hargapokok produksi variabel per proses produksi sebesar Rp4.608.000,00. Penerimaan per proses produksi sebesarRp12.972.602,00 harga pokok produksi variabel selama bulan Juni 2008 sebesar Rp58.490.000,00. Penerimaanselama bulan Juni 2008 Rp379.188.060,00. Harga pokok produksi per unit tidak dapat diketahui karena bentukproduk tempe yang beragam, sampai 18 bentuk kemasan, namun harga rata-rata keseluruhan sebesar Rp263,00per unit. Rata-rata pendapatan pengrajin Rp225.061,00 per proses produksi. Rata-rata pendapatan selama bulanJuni 2008 sebanyak Rp6.643.877,00.Kata kunci: pengrajin tempe, buruh wanita, kenaikan harga kedelai  ABSTRACTThis research intent to: 1 ). Effort that done by tempe's worker and its female labor and its effort aftereffect of increase of price soybean at Kembaran's district. 2 ). Increase labors laboring absorption female andaccepted pay on tempe's worker at Pliken Village Kembaran District. 3 ). Cost of goods manufacture pertempe's industrial unit and production factor price that is utilized at Kembaran's District after effect of increaseof price soybean. 4 ). Cost and tempe's industry operating revenues Kembaran's District after effect of increaseof price soybean. Basic method in observational it is methodic survai. Research is done at Pliken VillageKembaran District, Banyumas Regency. Sampling unit that is taken is producer tempe and its woman labour.Farmer sample take did by method Simple Random is Sampling gotten total sample as much 31 tempe's worker.Observational result that Effort that done by tempe's worker and its effort after effect of increase of pricesoybean is fixed sells at the price same but size tempe is minimized or reduces content per packages tempe;partly worker utilizes cassava mixture; reduce total fieldwork energy hour family; raising tempe's sell price.Female labor tempe 70 percent, still working as tempe's packer and 30 percent adds other works as field handor seller. Labor pay to be accounted by man days, with averagely job 4 hours / days. Working woman laboron every producer average 3 person, averagely get Rp6000,00. Variable cost of goods manufacture perprocesses as big as Rp 4.608.000,00. Acceptance per production process as big as Rp12.972.602,00 variableCost Of Goods Manufacture up to month of June 2008 as big as Rp 58.490.000,00. Acceptance up to month ofJune 2008 Rp379.188.060,00. Cost of goods manufacture per unknowledgeable unit because tempe's productform that medley, until 18 pack forms, but overall average cost as big as Rp263,00 about unit. Averagelypropertied producer Rp225.061,00 about production process. Averagely propertied up to month of June 2008 asmuch Rp6.643.877,00.Key word : tempe′s worker, women labour, dan increase of price soybean
MEDIA PEMBAWA ALTERNATIF INOKULAN MIKROBA PELARUT FOSFAT BERBASIS LIMBAH PERTANIAN Tamad, Tamad; Maryanto, Joko
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.112

Abstract

Mikroba Pelarut Fosfat (MPF) sudah lama digunakan untuk meningkatkan efisiensi pemupukan P.Perakitan MPF dalam bentuk inokulan yang murah dan mudah diaplikasikan perlu dikembangkan. Penelitian inibertujuan untuk menguji laju tumbuh dan daya pelarutan fosfat dari Pseudomonas sp., dan Aspergillus sp. padapembawa padat dan cair serta lama inkubasi dalam pembawa tersebut. Percobaan terdiri dari tiga faktor yangdisusun dalam rancangan petak terbagi. Petak utama ialah MPF dengan taraf: Pseudomonas sp. (bakteri), danAspergillus sp. (jamur). Anak petak ialah media pembawa dengan taraf: pembawa padat, dan pembawa cair.Anak-anak petak ialah komposisi media pembawa dengan 4 taraf. Pembawa padat berupa campuran abu sekam(AS), dedak padi (DP), dan onggok tapioka (OT), terdiri atas: 30 % AS dan 30 % DP, 30 % AS dan 30 % OT,30 % DP dan 30 % OT, dan 20 % AS, 20 % DP, dan 20 % OT dengan 40 % air; dan pembawa cair berupamolase terdiri atas: 25 %, 50 %, 75 % dan 100 % volume molase. Inkubasi inokulan selama 4 minggu dan 8minggu. Laju tumbuh Pseudomonas sp. (0,75 UPK/ml/hari) lebih cepat dibanding Aspergillus sp. (0,54UPK/ml/hari). P terlarut oleh Aspergillus sp. (21,41 ppm) lebih banyak dibanding Pseudomonas sp. (9,98 ppm)dan P terlarut oleh bakteri dan jamur pelarut fosfat asal pembawa padat (20,27 ppm) lebih banyak dibandingasal pembawa cair (8,76 ppm), sedangkan P terlarut oleh Pseudomonas sp., dan Aspergillus sp. antara inkubasi4 minggu dan 8 minggu sama. Perbedaan pembawa padat dan cair tidak mempengaruhi laju tumbuhPseudomonas sp., dan Aspergillus sp. Inkubasi 8 minggu menurunkan laju tumbuh Pseudomonas sp. danAspergillus sp. Pembawa padat terbaik untuk Pseudomonas sp., dan Aspergillus sp. isolat Ajibarang adalah 20% Abu Sekam, 20 % Dedak Padi, dan 20 % Onggok Tapioka dan 25 % Molase untuk pembawa cair.Kata kunci: mikroba pelarut fosfat, media pembawa, inokulan, limbah pertanian ABSTRACTPhosphate Solubilizing Microorganisms (PSM) has been used for increasing P fertilization efficinencyfor a long time. The research of carrier for PSM inoculants effective need to be developed. The researchaimed to evaluate the viability and the ability of phosphate solubilization of Pseudomonas sp., and Aspergillussp. in solid and liquid carrier and incubation time. The research arrange on Split-split Plot Design, with threefactors. The main factor is PSM with two levels are bactery (Pseudomonas sp.) and fungy (Aspergillus sp.) ofphosphate solubilizing. The sub plot is carrier wit level solid and liquid carrier. The sub-sub plot is 4 levels ofcarrier. Solid carrier are hush ash (HA), rice waste (RW) and tapioca waste (TW), with composition: 30 % HAand 30 % RW, 30 % HA and 30 % TW, 30 % RW and 30 % TW and 20 % HA, 20 % RW and 20 % TW with 40% is water; and liquid carrier is molase with concentration: 25 %, 50 %, 75 % and 100 % volum molase.Incubation time of innoculant is 4 weeks and 8 weeks. The growth rate Pseudomonas sp. (0.75 CFU/ml/day)faster than Aspergillus sp. (0.54 CFU/ml/day). The soluble P by Aspergillus sp. (21.41 ppm) is greater thanPseudomonas sp. (9.98 ppm) and the soluble P by Pseudomonas sp., and Aspergillus sp. from solid carrier(20.27 ppm) greater than liquid carrier (8.76 ppm), while the incubation time could not affect the soluble P byPseudomonas sp., and Aspergillus sp. The different between solid and liquid carrier could not affect the growthrate of Pseudomonas sp., and Aspergillus sp., however, 8 weeks incubation could decrease the growth rate.The best solid carrier for isolate Pseudomonas sp., and Aspergillus sp. of Ajibarang is 20 % of hush ash, 20 %of rice waste and 20 % of tapioca waste and 25 % of molase for liquid carrier.Key words: phosphate solubilizing microorganims, inoculants, carrier, agriculture waste 
EFISIENSI EKSPRESI GEN GUS MENGGUNAKAN PROMOTER CaMV35S DAN RUBQ2 PADA TEBU TRANSGENIK Pudjiwati, Eko Hary
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2010.14.2.108

Abstract

Promoter adalah salah satu faktor penting yang harus dipertimbangkan dalam transformasi genetik,karena promoter menentukan tingkat ekspresi gen yang ditransfer. Gen Gus merupakan gen pelapor yang banyakdigunakan dalam proses transformasi genetik untuk mengetahui aktivitas suatu promoter. Penelitian ini bertujuanuntuk mengetahui efisiensi ekspresi transien gen GUS pada tebu transgenik menggunakan promoter CaMV35 Sdan RUBQ2. Hasil penelitian menunjukkan ekspresi transien GUS hanya terdeteksi pada tebu transgenikmenggunakan promoter RUBQ2 dengan efisiensi 14%.Kata kunci: GUS, promoter, CaMV35S, RUBQ2  ABSTRACKPromoter is one of important factor which must be considered in genetic transformation, becausepromoter determinelevel of the transferred gene expression. GUS gene is the reporter gene which is a lot ofused in genetic transformation to know the activity of an promoter. This research aim to to know the efficiencyof transient GUS expression in transgenic sugarcane use CaMV35 S and RUBQ2 promoter. Result of researchshow transient GUS expression only detected in transgenic sugarcane use the RUBQ2 promoter with theefficiency 14%.Key words: GUS, promoter, CaMV35S, RUBQ2
GENETIKA SIFAT KETAHANAN CABAI MERAH TERHADAP VIRUS ChiVMV Noor Farid; Darini Sri Utari
Agrin Vol 14, No 2 (2010): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.202 KB) | DOI: 10.20884/1.agrin.2010.14.2.110

Abstract

Produksi cabai merah terkendala adanya hama dan penyakit tanaman. Virus ChiMV (Chilli Veinal MottleVirus) adalah salah satu penyakit tanaman yang dapat menurunkan hasil cabai merah. Perakitan hibrida cabaimerah yang hasil tinggi dan tahan penyakit virus ChiVMV diperlukan informasi antara lain daya gabung umum,khusus, heterosis, letak gen dan heritabititas. Penelitian ini bertujuan untuk : 1). mendapatkan nilai daya gabungumum dan khusus, 2). memperoleh nilai heterosis dari sejumlah persilangan yang ada, 3). memperoleh nilaiheritabilitas dari sifat ketahanan terhadap virus ChiVMV. Hasil penelitian adalah 1) genotip cabai merah yangyang sesuai untuk tetua tahan ChiMV berdasarkan DGU intensitas penyakit: G6321 dan X4271, 2) genotip cabaimerah yang berdasarkan DGK intensitas penyakit yang tahan: X4271xG6321, dan G3257xX427, 3) berdasarkannilai DGK, heterosis, intensitas penyakit, dan bobot buah bahwa persilangan antaraX4271xG6321,G3257xx4271 dan G3257x Jatilaba dipilih sebagai genotip tahan ChiMV dan hasil tinggi 4) nilaiheritabilitas arti luas termasuk tinggi tetapi heritabilitas arti sempit berkisar antara tinggi sampai rendah, dan 5)sifat ketahanan terhadap virus ChiVMV dipengaruhi tetua betina.Kata kunci : daya gabung, heterosis, heritabilitas, ketahanan virus ChiVMV, efek tetua betina ABSTRACTYield of chilli pepper is limited by disease and insect pests. ChiMV virus is off important disease thatcould decrease chilli pepper production. Genetic information, such as genetic parameter, combining ability,heterosis and heritability is required in order to improve resistance variety. The research’s objectives were: 1)to estimate the general combining ability (GCA) and specific combining ability (SCA), 2) to estimate theheterosis of several diallel mating, 3) to estimate heritability of resistance to ChiMV virus. It was evidenced that:1) genetype of chilli pepper having low GCA (desease intensity) were G6321 and X4271, 2) genetypes of chillipepper resulted from diallel mating of X4271xG6321, dan G3257xX4271 low high SCA on desease intensity toChiMV virus, 3) base on SCA, heterosis, disease intensity, fruit weigh, X4271xG6321,G3257xx4271 danG3257x Jatilaba genotypes could be selected for high yielding chili and resistance to ChiMV virus, 4) boardsense heritability values of observed character were high, but range of narrow sense heritability were high tolow, 5) resistance character to ChiMV virus was influenced by maternal effect.Key words: combining ability, heterosis, heritability, ChiMV resistance, maternal effect

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