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ILMU KELAUTAN: Indonesian Journal of Marine Sciences

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Published by Universitas Diponegoro

ISSN : 08537291 EISSN : 24067598 DOI : -

Core Subject : Science,

Earth & Planetary Sciences

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and oceanography.

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Articles 743 Documents

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Effects Of pH, Temperature And Salinity In Growth And Organic Acid Production Of Lactic Acid Bacteria Isolated From Penaeid Shrimp Intestine Subagiyo Subagiyo; Sebastian Margino; Triyanto Triyanto; Wilis Ari Ari Setyati1,2
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 4 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Bakteri asam laktat telah lama dikembangkan sebagai probiotik. Penentuan kondisi lingkungan yang optimum untuk pertumbuhan sel serta asam organik memberikan gambaran aktivitas optimum untuk kinerja probiotik baik dalam sistem fisiologi inang maupun dalam sistem bioproses untuk produksi sel dan metabolit. Penelitian ini bertujuan untuk mengetahui pengaruh faktor lingkungan (pH, suhu dan salinitas) terhadap pertumbuhan dan produksi total asam organik tiga isolat bakteri asam laktat yang telah diseleksi dari intestinum udang penaeid. Eksperimen menggunakan medium deMan, Rogosa and Sharpe (MRS) cair. Perlakuan pH awal meliputi nilai pH 4, 5 dan 6. Perlakuan suhu meliputi suhu 25, 30 dan 35OC serta perlakuan salinitas meliputi salinitas 0,75 %, 1,5 % dan 3 %. Setiap interval 6 jam dilakukan pengambilan sampel kultur bakteri dan penghitungan pertumbuhan berdasarkan perubahan optical density (pada panjang gelombang 600 nm) sedangkan produksi asam laktat dianalisis dengan metode titrimetrik menggunakan NaOH 1 N sebagai larutan titrasinya. Berdasarkan hasil penelitian disimpulkan bahwa suhu, pH awal dan salinitas berpengaruh terhadap pertumbuhan dan produksi asam organik. Nilai kondisi lingkungan terbaik untuk pertumbuhan dapat berbeda dengan nilai terbaik untuk produksi asam organic. Hal ini ditunjukan oleh nilai laju pertumbuhan dan produksi asam laktat tertinggi dari tiga isolat uji terjadi pada suhu, pH awal dan salinitas yang berbeda. Isolat L12 tumbuh optimum pada suhu 30oC, pH awal 6 dan salinitas 0,75%. Isolat L14 tumbuh optimum pada suhu 30oC, pH awal 6 dan salinitas 1.5%. Isolat L 21 tumbuh optimum pada suhu 30 oC, pH awal 6 dan salinitas 1.5%. Kata kunci: bakteri asam laktat, suhu, pH, salinitas, asamorganik, pertumbuhan, Lactic acid bacteria are widely distributed in intestinal tracts of various animals where they live as normal flora.Strains of lactic acid bacteria are the most common microbes employed as probiotics, The optimum condition for growth are important to mass production and to determined parameters most suitable for growth. The effects of temperature, pH and salinity on the growth and production of lactic acid from the three shrimp intestinal lactic acid bacteria isolates were conducted using bacth culture in a flask. These variables for growth were determined based on the growth curves and lactic acid production. Data from the flask batch experiment demonstrated that the best initial pH and temperature for growth of isolat L12 ,L14 and L21 were found to be pH 6 and 30 OC. Salinity (NaCl concentration) 0,75% were the best for growth of isolat L12. Salnity 1,5 % were best for growth of isolat L14 and L21. Key words : growth, temperature, pH, salinity, lactic aid bacteria

Kandungan Asam Lemak Omega-3 (Asam Linolenat) pada Kerang Totok Polymesoda erosa yang diberiPakan Tetraselmis chuii dan Skeletonemacostatum Endang Supriyantini; Ita Widowati; Ambariyanto Ambariyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 2 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Percobaan ini dilakukan untuk mendapatkan informasi tentang pengaruh pemberian pakan alami Tetraselmischuii dan Skeletonema costatum terhadap kandungan asam lemak omega-3 (asam linolenat) pada kerang Totok Pofymesoda erosa. Kerang Totok yang digunakan berukuran 5-6 cm berasal dari perairan P. Gombol, Segara Anakan, Cilacap. Metode yang digunakan adalah eksperimental laboratorium dengan Rancangan Acak Lengkap (RAL) dengan 2 faktor dan 3 ulangan. Faktor pertama adalah pakan alami (A) dengan 3 perlakuan yaitu T. chuii dengan konsentrasi 45x Iff sel/ml(Al); S. costatum dengan konsentrasi 45 x Iff sel/ml (AZ); dan kombinasi dari kedua pakan tersebut dengan konsentrasi 22.5 x iff sel/ml T. chuii: 22.5 x Iff sel/ml S. costatum (A3). Faktor kedua yaitu periode waktu sampling (T) terdiri dari 2, 4, 6, dan 8 hari. Metode Gas Liquid Chromatography (GLC) digunakan untuk menentukan kandungan asam lemak omega-3. Semua datayang diperoleh dianalisis dengan menggunakan uji Anova dua jalur dengan SPSS. Hasil penelitian menunjukkan bahwa, jenis pakan alami berpengaruh nyata terhadap kandungan asam lemak omega-3 (asam linolenat) (Ftest= 5.409; p= 0.012). Sedangkan waktu periode sampling tidak berpengaruh nyata terhadap kandungan asam lemak linolenat pada kerang Totok P. erosa (F test= 0.795 ; p= 0.509). Perlakuan pakan alami dan waktu periode sampling memberikan pengaruh secara bersama terhadap kandungan asam lemak linolenat kerang Totok P. erosa (F test= 3.535; p= 0.012). Disimpulkan bahwa kualitas pakan, sifat dan komponen dinding sel penyusun kedua pakan alami tersebut diduga berpengaruh terhadap kandungan asam lemak linolenat pada kerang Totok P. erosa.Kata kunct: Kerang Totok Pofymesoda erosa, Tetraselmis chuii, Skeletonema costatum. Asam lemak linolenat.

Gastropod Density and Diversity in the Mangrove Forest of Pagatan Besar Village, Tanah Laut Regency, Indonesia Bimo Aji Nugroho; Mochamad Arief Soendjoto; Muhammad Zaini
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 4 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Public awareness and knowledge on mangrove forest biodiversity, especially gastropods, is very limited. This study aims to compare the density and diversity index of gastropod species according to their distance from the coastline and to analyze the relationship of the two with the physical environment around them. Three lanes were placed in the southern part of the mangrove forest. The first lane (LT-1) was placed about 5 m from the coastline towards the sea. The second lane (LT-2) was placed 10 m towards the sea from LT-1 and the third lane (LT-3) was 10 m from LT-2 towards the sea. In each lane there were 10 (1 mx 1 m) plots and the distance between adjacent plots was 10 m. A total of 1.432 Gastropod individuals consisting of 16 species and 7 families were collected from 30 plots. In detail 16 species (927 individuals) were collected from LT-1, 16 species (500 indv) from LT-2, and only 3 species or 5 individuals from LT-3. The density of LT-1, LT-2, and LT-3 species respectively was 92.7; 50.0; and 0.5 indv.m-2, while the diversity index successively was 2.56 (moderate); 2.53 (moderate); and 0.95 (low). Both species density and diversity in lanes that are close to the coastline are higher than in lanes located in the direction of the sea. The base substrate on the path that was close to the coastline was clay, while on the lane that laid in the direction of the sea was sandy loam.

Isolasi dan Seleksi Bakteri Penghasil Enzim Ekstraseluler (proteolitik, amilolitik, lipolitik dan selulolitik) yang Berasal dari Sedimen Kawasan Mangrove (Isolation and Selection of Extracellular Enzyme Producing Bacteria Originating from Mangrove Sedimen Wilis Ari Setyati; Subagiyo Subagiyo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Bioremediasi tambak udang memegang peranan penting dalam upaya membersihkan tambak dari bahan pencemar internal yang dihasilkan selama proses budidaya itu sendiri. Proses budidaya udang merupakan kegiatan yang potensial menghasilkan limbah organik terutama berasal dari sisa pakan dan hasil ekskreta (feses). Bakteri heterotrofik memiliki kemampuan untuk menghasilkan enzim ekstraseluler yang diperlukan untuk bioremediasi limbah organik. Penelitian ini ditujukan untuk mengisolasi bakteri heterotrofik yang berasal dari ekosistem mangrove utuk dikembangkan sebagai agensia bioremediasi limbah organik pada tambak udang. Sedimen mangrove diperoleh dari kawasan mangrove Segara Anakan- Cilacap dan kawasan Mangrove Kaliuntu-Kabupaten Rembang. Isolasi dilakukan dengan metode agar tuang menggunakan media Zobell agar. Pengujian aktivitas proteolitik, amilolitik, lipolitik dan selulolitik dilakukan dengan metode diffusion agar menggunakan paper disk berturut-turut pada media Zobell agar yang diperkaya dengan 1% skim milk, 1% amilum, dan 1% tween 80 dan 1 % CMC. Diameter zone hidrolitik digunakan sebagai dasar untuk melakukan seleksi. Hasil penelitian diperoleh 35 isolat (16 isolat dari Kaliuntu – Rembang dan 19 isolat dari Segoro Anakan – Cilacap). Jumlah isolat yang mempunyai kemampuan menghasilkan enzim ekstraseluler berturut-turut 33 isolat dengan aktivitas proteolitik 25 isolat dengan aktivitas amilolitik, 29 isolat dengan aktivitas lipolitik, dan 12 isolat dengan aktivitas selulolitik. Hasil seleksi berdasarkan diameter zone hidrolitik diperoleh 10 isolat yang potensial untuk dikembangkan sebagai agensia bioremediasi.Kata kunci: proteolitik, amilolitik, lipolitik, selulolitik, mangrove, bioremediasi, tambak udangBioremediation of shrimp pond plays an important role in the effort to clean up the internal pollutants of pond produced during the cultivation process. Shrimp farming produces mainly organic waste from food remains and the excreta. Heterotrophic bacteria have the ability to produce extracellular enzymes required for the bioremediation of organic waste. This study aimed to isolate heterotrophic bacteria from mangrove ecosystem to be developed as agents of bioremediation of organic wastes in shrimp ponds. This was done by isolating and selecting bacteria producing extracellular enzymes of proteolytic, amylolitic, lipolytic and cellulolytic from mangrove sediments collected from the mangrove area of Segara Anakan at District of Cilacap and Kaliuntu, district of Rembang. Isolations were done by pour-plate method using a Zobell agar medium. Assays for proteolytic, amilolytic, lipolytic and cellulolytic activity were performed using paper disk on Zobell agar medium enriched respectively with 1% skim milk, 1% soluble starch, 1% tween 80 and 1% CMC. Hydrolytic zone diameter was used as the basis for selection of enzymatic activities. The results obtained was 35 isolates (16 isolates from Kaliuntu–Rembang dan 19 isolate froms Segara Anakan – Cilacap), consisting of 33 proteolytic enzyme, 25 amilolytic enzyme, 29 lipolytic enzyme and 12 isolates cellulolytic producing enzyme. Based on hydrolytic zone diameter were selected 9 isolates which potential to be developed as bioremediation agent.Key words: Bioremediation, proteolytic, amilolytic, lipolytic, selulolytic

Asam Lemak Dari Gonad Kerang Simping Amusium Pleuronectes Bambang Cahyono; Bayu Legowo; Jusup Supriyanto; Ita Widowati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 1 (2010): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Amusium pleuronectes merupakan salah satu biota laut yang sangat prospektif untuk dikembangkan. Beberapa senyawa asam lemak yang terdapat dalam bahan alam tersebut, untuk pertamakalinya telah berhasil diidentifikasi dan diusulkan strukturnya. Sampel A. pleuronectes dikumpulkan dari perairan Weleri - Kendal, Jawa Tengah. Isolasi bahan dilakukan dengan ekstraksi kontinyu, diikuti dengan hidrolisis dan esterifikasi menggunakan methanol/BF3. Analisis ester dari asam lemak dilakukan dengan GC-MS, menghasilkan tujuh puncak utama yang diusulkan sebagai asam tetradekanoat, asam pentadekanoat, asam heksadekanoat, asam heksadekaenoat, asam heptadekanoat, asam oktadekanoat dan asam oktadekaenoat, Senyawa omega-3 yang lazim diperoleh dalam bahan alam laut jenis lain, tidak ditemukan dalam sampel dari lokasi yang dipilh. Keadaan ini membuka telah wawasan bagi penelitian lebih lanjut, khususnya data dari daerah lain, guna mendapatkan gambaran peta potensi pengembangan A. pleuronectes di Jawa Tengah. Kata kunci : Amusium pleuronectes, asam lemak, gonad Amusium spleuronectes. is one of the marine biota that very prospective for further development. Several fatty acid compounds contained in this natural product, for the first time have been identified and proposed their structures. Samples were collected from the waters of Weleri - Kendal, Central Java. Materials isolation were carried out using continuous extraction, followed by hydrolysis and esterification using methanol/BF3. Analysis of fatty acid esters by GC-MS, resulting in seven main peaks which is proposed as tetradecanoic acid, pentadecanoic acid, hexa-decanoic acid, heptadecanoic acid, and oktadecanoic acid. Omega-3 compounds commonly found in other materials types of marine natural, not found in samples from locations selected. This situation opens perspectives This situation opens perspectives for further research, especially data from other regions, in order to get an picture map of A. pleuronectes potential development in Central Java. Key words: fatty acids, Amusium pleuronectes, gonad

Assessment of Sustainable Use of Coastal Resources of Regional Waters Conservation Area Biak Numfor Regency, Papua Province, Indonesia Sutaman Sutaman; Yusli Wardiatno; Mennofatria Boer; Fredinan Yulianda
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Efforts to exploit fish resources optimally, continuous and sustainable is an urgent demand for the greatest prosperity of the people, especially to improve the welfare of fishermen and fish farmers. The level of sustainable use of coastal resources in water conservation is very important, so that the utilization does not exceed the carrying capacity of the environment. The purpose of this study was to determine the level of sustainable use of coastal resources Biak Numfor, associated with the utilization of fisheries, aquaculture and tourism. The study was conducted in June to December 2015 and October to November 2016. The primary data obtained by interview and direct discussion through Focus Group Disscution (FGD) with fishermen community, tourist and tourist entrepreneurs as well as related officials in the Office of Fisheries and Marine Affairs, and Tourism Office of Biak Numfor Regency. Methods of data analysis approach sustainability analysis conducted by the method of MDS (Multi-Dimensional Scaling) with the help of software Rapfish. Based on the survey results revealed that the value of fisheries ordinated to achieve 57.66%, 44.80% aquaculture, and tourism 46.25%. With these achievements ordinated value, it can be concluded that the use of sustainable capture fisheries are still classified by the lever sustainability attributes include; the type of fishing gear, vessel types used and the catch per unit effort (CPUE). Meanwhile the relatively less sustainable aquaculture with the sustainability lever attributes include; cultivation technology, the number of business units with different types and species of fish. For tourism utilization is still considered less sustainable with levers sustainability attributes include the number of tourists, the type and number of amenities and facilities and infrastructure Keywords: Sustainability, utilization, waters conservation area (KKPD), MDS-Rapfish

Inventarisasi Spesies Ikan di Perairan Pantai Timur Kendari Fahmi Fahmi; Yuliadi Zamroni
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 4 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Perairan pantai Kendari menyimpan potensi sumberdaya laut yang cukup tinggi karena memiliki ekosistem pesisir yang beragam. Beberapa wilayahnya berfungsi sebagai daerah pemijahan dan asuhan ikan. Penelitian mengenai spesies ikan di perairan pantai Kendari dilaksanakan pada bulan Juli 2011 sebagai bagian dari kerjasama riset antara LIPI dengan Dikti dengan menggunakan Kapal Riset Baruna Jaya VIII. Tujuan penelitian ini adalah untuk mengidentifikasi kekayaan spesies ikan di perairan pantai Kendari dan menganalisis peranan tiap tipe perairan pantai di wilayah Kendari sebagai daerah pemijahan, pembesaran dan mencari makan ikan. Sebanyak 2010 individu ikan pantai diperoleh dari delapan lokasi penarikan jaring, yang terdiri dari 80 spesies, 53 genus dan 34 famili. Spesies ikan yang cukup memiliki kelimpahan tertinggi di perairan pantai Kendari antara lain ikan lingkis, Siganus canaliculatus (Siganidae) dan ikan petek, Eubleekeria splendens (Leiognathidae). Sedangkan ikan yang umum dijumpai di perairan ini antara lain adalah Achreichthys tomentosus dan Pentapodus trivittatus dengan luas sebaran meliputi 50% dari wilayah sampling. Secara umum, perairan pantai Kendari terbagi ke dalam empat kelompok tipe habitat berdasarkan pengelompokan dari komposisi spesies ikannya. Kata Kunci: Ikan, Perairan Pantai, Kendari, kelimpahan The coastal area of Kendari is known to have a potential marine resource due to the diversity of coastal ecosystems. Some of its areas are known as spawning and nursery ground for fishes. A study on coastal fishes of Kendari waters was conducted in July 2001 as a part of the joint research between LIPI and Dikti using The Baruna Jaya VII Research Vessel. The aim of the study was to find out the species richness and diversity of coastal fishes in Kendari waters, and to identify the role of each type of habitats in Kendari coastal area as spawning, nursery and feeding ground for fishes. A total of 2010 fishes were collected during the study, consisting of 80 species, 53 genera and 34 families. The most abundant species was Siganus canaliculatus (Siganidae) and Eubleekeria splendens (Leiognathidae). While the most common fishes in the area were Achreichthys tomentosus and Pentapodus trivittatus that were found at 50% of sampling area. In general, Kendari coastal area is grouped into four type of habitat regarding to fish species composition. Key words: Fishes, Coastal Waters, Kendari, abundance

Kelimpahan Fitoplankton di Padang Lamun Buatan (Artificial Seagrass Bed as Phytoplankton Habitat) Ita Riniatsih; Widianingsih Widianingsih; Sri Redjeki; Hadi Endrawati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 2 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Padang lamun berperan penting dalam menjaga kelestarian berbagai jenis organism laut. Namun secara umum kondisi ekosistem lamun saat ini semakin menurun. Melalui pengembangan padang lamun buatan diharapkan dapat membantu mengembalikan fungsinya, termasuk pertumbuhan fitoplankton sebagai epifit pada salah satu orgasnime yang berasosiasi di dalamnya. Penelitian ini bertujuan untuk melihat keberhasilan penciptaan habitat fitoplankton di padang lamun buatan. Penelitian dilakukan dengan dua model lamun buatan yang terbuat dari tali kalas, tanaman plastik berbentuk semak, dan transplantasi lamun asli jenis Enhalus acoroides serta padang lamun asli sebagai control dengan empat kali ulangan. Fitoplankton yang diperoleh selama penelitian sebanyak 30 jenis. Jenis fitoplankton yang mendominansi semua perlakuan adalah kelas Bacillariophyceae yaitu genus Nitzschia, Coscinodiscus, Bidulphia, Rhizosolenia dan Skeletonema. Jumlah jenis dan kelimpahan fitoplankton yang tertangkap terlihat berbeda di setiap sampling pengamatan. Hingga akhir pengamatan jumlah jenis dan kelimpahan fitoplankton tidak terlihat perbedaan antara lamun buatan dan padang lamun asli. Indeks keanekaragaman dan keseragaman fitoplankton dalam kategori sedang pada ketiga perlakuan. Hasil penelitian menunjukkan bahwa padang lamun buatan sama efektifnya dengan padang lamun asli dalam menyediakan tempat untuk penempelan fitoplankton sebagai epifit serta meningkatkan produktivitas primer di ekosistem padang lamun. Kata kunci: habitat, fitoplankton, padang lamun buatan Seagrass bed is one of coastal area ecosystems, which has important role for various marine organisms. Artificial seagrass bed can create new habitat for phytoplankton as one of epiphyte organisms which is associated with others marine organisms. The purpose of this research is to know successful a phytoplankton growth in artificial seagrass bed at Teluk Awur Coastal area, Jepara.. There were three treatments in this research i.e. (a) artificial seagrass bed from kalas rope, (b) artificial seagrass made from plastic, (c) seagrass of Enhalus acoroides, and natural seagrass bed ecosystem as control, with 4 replications. Thirty phytoplankton species were found in that area. Several genera from class Bacillariophyceae dominanted at all treatments, i.e. Nitzschia, Coscinodiscus, Bidulphia, Rhizosolenia and Skeletonema. There were differences on the number of species and abundance of phytoplankton every time sampling. However, the number of species and abundance of phytoplankton were similar between artificial seagrass bed and natural seagrass bed at the end of this research. All treatments have medium category for diversity and evenness index. The results suggest that artificial seagrass bed is as effective as natural seagrass for location (medium) for phytoplankton habitat and for increasing primary productivity. Keywords : habitat, phytoplankton, artificial seagrass bed

Aplikasi Perbedaan Komposisi N, P dan K pada Budidaya Eucheuma cottonii di Perairan Teluk Awur, Jepara Edi Wibowo Kushartono; Suryono Suryono; Endah Setiyaningrum
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 3 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Rumput laut (Eucheuma cottonii) merupakan salah satu hasil laut yang memiliki nilai ekonomis tinggi yang ditunjukkan dengan meningkatnya permintaan dalam berbagai bentuk sehingga diperlukan adanya usaha peningkatan produksinya. Studi aplikasi pupuk N, P dan K dengan konsentrasi yang berbeda telah dilakukan pada usaha budidaya rumput laut tersebut melalui eksperimental lapangan, dengan Rancangan Acak Lengkap. Bibit E.cottonii direndam dalam pupuk selama 15 menit sebelum ditanam dengan sistim rakit. Terdapat lima kombinasi komposisi N, P, dan K yaitu 10:55:10; 15:15:15; 46:0:0; 60:20:10; dan Kontrol (tanpa direndam dalam pupuk). Bobot awal bibit rumput laut adalah 80 gram dan pengamatan pertumbuhan berat mingguan dilakukan selama 5 minggu. Hasil pengamatan menunjukkan bahwa rerata bobot total dan laju pertumbuhan tertinggi dicapai pada perlakuan perbandingan N,P dan K 60:20:10 yaitu 543,33 gram dan 5,47 % dan yang terendah adalah 326,67 gram dan 4,02 % yang dicapai perlakuan perbandingan N,P dan K sebesar 10:55:10 Kata kunci : Euchema cottonii, N, P, K, pertumbuhan Eucheuma cottonii, seaweed, is one of marine products with high economic value which can be seen from increasing market demand. The study of using different commercial fertilizer in seaweed aquaculture is one of possible effort to increase seaweed production. The method used in this research was experimental applying randomized complete design. All samples are deeped for 15 minutes in different composition of NPK fertilizers before cultivated in floating raft method. The treatments are NPK ratio of 10:55:10; 15:15:15; 46:0:0; 60:20:10 and without fertilizer as control. Seaweed mass for cultivation was 80 grams observe at every week during five week. The observation result show that the highest average of total biomass reached and Survival growth rate at NPK ratio treatment 60:20:10 is 543.33 grams and 5,47 % ,and the lowest is 326.67 grams 4,02 % reached by N, P, and K ratio treatment was 10:55:10 Key words : Euchema cottonii, N, P, K, growth

Spatial and Seasonal Distribution of Cadmium and Lead in Sediment, Water and Its Response of Metal Transcription Factor-1 in Cardinal Fish Apogon beauforti Dominggus Rumahlatu; Estevanus Kristian Huliselan; Sriyanti Imelda A Salmanu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 1 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Cadmium (Cd) and lead (Pb) are known to have high toxic effects and have been found polluting marine environment. Regular monitor the concentration of heavy metals in marine waters is necessary by combining chemical and biological analysis. This research was conducted to analyze the levels of heavy metals in sediment, water column, cardinal fish Apogon beauforti, and to measure the concentration of MTF-1 protein in A. beauforti. This research was conducted at four sampling stations at Ambon bay for 2 years. The levels of Cd and Pb metals were analyzed using AAS instruments (Atomic Absorbsion Spectrophotometer), while the concentration of MTF-1 protein in the body of A. beauforti was analyzed using indirect ELISA. The results showed that the seasonal and spatial distribution of heavy metals Cd and Pb at Ambon bay did not show any variation. The results of the ANOVA proved that the concentrations of heavy metals Cd and Pb in the four research stations were not significantly different in the year 2013 and in the year 2014 in the samples of sediment, the water column, and the body of A. beauforti. In addition, it was revealed that the concentration of MTF-1 protein of A. beauforti in 2014 was higher than that in 2013, with an average of the concentration of MTF-1 protein of A.beauforti was higher at station 2, which was at Ferry Galala Harbor. Keywords: distribution, spatial, seasonal, cadmium, lead, MTF-1, Apogon beauforti

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