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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 64 Documents
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Search results for , issue "Vol 10, No 2 (2018): August 2018" : 64 Documents clear
The Impact of Lampenflora on Cave-dwelling Arthropods in Gunungsewu Karst, Java, Indonesia Kurniawan, Isma Dwi; Rahmadi, Cahyo; Ardi, Tiara Esti; Nasrullah, Ridwan; Willyanto, Muhammad Iqbal; Setiabudi, Andy
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13991

Abstract

The development of wild caves into show caves is required an installation of electric lights along the cave passages for illumination and decoration purposes for tourist attraction. The presence of artificial lights can stimulate the growth of photosynthetic organisms such as lampenflora and alter the typical cave ecosystem. The study was aimed to detect the effect of lampenflora on cave-dwelling arthropods community. Four caves were sampled during the study, 2 caves are show caves with the existence of lampenflora and 2 others are wild caves without lampenflora. Arthropods sampling were conducted by hand collecting, pitfall trap, bait trap and berlese extractor. Lampenflora comprises of algae (Phycophyta), moss (Bryophyta) and fern (Pteridophyta) grow mostly around white light lamps. Richness, diversity, and evenness indices of Arthropods are higher in caves with the existence of lampenflora compared to caves without lampeflora. This study clearly shows that the presence of lampenflora can increase Arthropods diversity and suppress dominancy of common Arthropods species in caves, also increasing the relative abundance of predators. This condition will shift the ecosystem equilibrium and lead to cave ecosystem destruction. The results of this study should be a scientific consideration for show cave development and management. Lampenfloras have to be removed from all caves and preventive efforts should be taken to minimize their growth.
The Potency of Black Soldier Larvae (Hermetia illucens L.) as a Source of Protein for Livestock Feed Supriyatna, Ateng; Kurrahman, Opik Taufik; Cahyanto, Tri; Yuliawati, Astri; Kulsum, Yuni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14422

Abstract

Black Soldier fly larvae is an insect capable to convert organic waste into body biomass. The purpose of this research was to determine the productivity, mass balance of the consumption process, and content of protein and fat of prepupa which has a potency as a livestock feed. This research used 6-day-old larvae, they were fed with cassava skin that has been smoothed with a variety of feeding doses: 12.5; 25; 50; 100; and 200 (mg / larvae / day) with ratio of feed and water 2: 1. The results showed that the feeding of 200 mg / larva / day produced the best larval growth with biomass productivity of 1.54 mg / day, residue yielded of 67.1 mg / day. The organic waste was convertion into larvae biomass. In this study, approximately consumption process scale is feed successfully converted to biomass 2.77%, metabolism 6.98%, and 90.24% residue. Protein content is 25.7%. It can be concluded that the larvae are able to convert organic waste into high protein biomass and potentially being used as a livestock feed. This research acts a model to predict mass balance of substrates for feed. The benefit of this research is the science approach used in this study that can be used as a reference by researchers and the community to determine the biomass production of an organism.
Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. Yuniarsih, Windra; Halimatul, Heli Siti; Wulandari, Asri Peni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14891

Abstract

Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.
Problem-Based Learning Module of Environmental Changes to Enhance Students’ Creative Thinking Skill Anjarwati, Pamula Guruh Prastiwa; Sajidan, Sajidan; Prayitno, Baskoro Adi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.12598

Abstract

Creative thinking skills are important for long-term success. Empowering creative thinking skills which can be trained through problem-solving activities in learning, thereby promoting high cognitive engagement. This study aimed to test the effectiveness of the research and development product of Problem-Based Learning module of environmental changes in empowering students’ creative thinking skills. The research method used was the Quasi-experiment with nonequivalent control group design. The sample of the research was the tenth-graders of a Senior High School in Karanganyar district, Central Java. The sampling was cluster sampling by involving two classes, namely the Grade X-MIA 1 with 28 students and the X-MIA 4 with 28 students. The qualitative and quantitative-descriptive approaches were used to analyze the data. The result shows the mean of creative thinking skills score of experiment group is higher than the control group. This developed module could empower students’ creative thinking skills by problem-solving activities as needed for Indonesian educational goal . The study concludes that development module was constructed based on Problem-based Learning activities in the module could stimulate students solved and identified the problems with their ideas and developed their creative thinking.
Identification and Cluster Analysis of Pitcher Plant (Nepenthes spp.) from South Sumatera Indonesia Lestari, Weni; Jumari, Jumari; Ferniah, Rejeki Siti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13968

Abstract

Nepenthes spp. is a typical plant of Southeast Asia especially Indonesia which has a special leaf modification called a pitcher. The largest number of Nepenthes spp. species in Indonesia is on the island of Sumatra. The purpose of this reseach was to identify and analyze cluster Nepenthes spp. from South Sumatra based on morphological characteristics. The specimens were collected from the forest of Tekorejo Village, Air Itam Village and cultivation location in Palembang city of South Sumatra. Identification of morphological characters performed on the characteristics of root, stem, leaves, and pitcher. The morphological data is used for cluster analysis using NTSYS software version 2.02. The identification results showed 9 variants of Nepenthes spp. which belong to the species N. mirabilis, N. gracilis, and N. sumatrana. Dendogram analysis results form two main clusters with a similarity value of 22%. The first cluster consists of N. mirabilis and N. sumatrana. The second cluster consists of N. gracilis. Based on the results of this study can be concluded that the species Nepenthes spp. South Sumatra is N. mirabilis, N. gracilis, and N. sumatrana. The results of this study will be dedicated to updating information about the existence of Nepenthes spp. from South Sumatra and his cluster.
Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Ichsan, Ichsan; Boediono, Arief; Eriani, Kartini; Suryani, Irma; Azhar, Al; Nursanti, Risa
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Abstract

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbeccos Modified Eagles Media (mDMEM) and incubated overnight at 37 °C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 °C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.
Development of Science Learning Tool Based on Problem Based Learning with Google Classroom to Improve Argumentation Skill Pritasari, Ade Cyntia; Jumadi, Jumadi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14320

Abstract

Using science learning tools will optimize the learning process. Good learning process will train the skill needed in the 21st century, including the skills of argumentation. From this point of view, an science learning tool based on problem based learning with google classroom has developed in this study. This study aimeds to test the appropriateness of science learning tool and the effectiveness of science learning tool developed in improving argumentation skill. This study was an research and development (R&D) research using 4D method from Thiagarajan with four stages including define, design, develop, and disseminate. The product trials have been conducted in 7th grade students of State Junior High School 2 Magelang at second semester of year 2017/2018. The data collection was done by non-test and test technique. Based on the results of the research note that learning tools developed theoretically declared valid based on expert assessment with very good category and effective in improving students’ argumentation skill. Learning tool based on problem based learning with google classroom is a new approach to learning with the capability to the transform education in a better quality. Implementation of learning tool based on problem based learning with google classroom provide new alternatives in improving the quality of science education achievement.
Bryophytes in Cibodas Botanical Garden: Diversity and Potential Uses Nadhifah, Ainun; Khujjah, Madinatul; Vitara, Pantalea Edelweiss; Noviady, Ikhsan
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14433

Abstract

Research on the diversity of bryophytes in Indonesia has been generally accomplished. However, the publication of potential uses of this tiny plant is very limited. Cibodas Botanical Garden (CBG) as a center for ex-situ plant conservation has potential climate conditions as a location of moss growth. The study aims to collect bryophytes in CBG and to identify their potential uses. Purposive sampling method was used on various substrate (soil, rocks, trees). Some environmental parameters were also recorded, for instance, substrate type, temperature, and humidity. All of bryophytes collections were taken to the laboratory for the identification and saved in herbarium (CHTJ). A literature study was conducted to determine the potential uses of identified moss species. From 153 collection numbers recorded in CBG, there were 42 species which have potential use. In addition, there were 42 species that have the potential to be used as a medicinal plant, ornamental plants, uses in agriculture and environmental services. This study provides the initial information about the potential uses of mosses, liverworts, and hornworts particularly in Indonesia. Furthermore, these prospective utilization encourage the innovative research in Indonesia, particularly on alternative natural resources.
The Potential of Microbial Symbionts Macrotermes gilvus Hagen Termite Gut as Degrading Agents of Cellulose in Bioethanol Production Susilowati, Dewi; Subekti, Niken; Bintari, Siti Harnina
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14965

Abstract

Water hyacinth is a potential feedstock for bioethanol production because of their high cellulose. The microbial symbionts of the Macrotermes gilvus termite’s gut have a high endoglucanase enzyme activity. This research was aimed to analyze the pH, temperature and agitation effects towards cell density, endoglucanase enzyme activity and reducing sugar, and to determine the effective optimum condition that can produce maximum reducing sugar. This research used central composite design (CCD) with the total number of run was . The independent variables were including pH (5.9, 6.4, 7.0, 7.6, 8.0), temperature (30 0C, 33 0C, 37 0C, 41 0C, 44 0C) and agitation (90 rpm,114 rpm, 150 rpm,185 rpm, 210 rpm), with six replications at central points. Parameters measured were cell density, endoglukanase enzyme activity and reducing sugar, thus analyzed by the statistical software package MINITAB 18.0. The Student’s t-test result showed the primary sequence influencing cell density as pH ˃ agitation ˃ temperature and towards endoglucanase enzyme activity and reducing sugar as pH ˃ temperature ˃ agitation, P ˂ 0.05. The maximum reducing sugar (60.13 ± 3.16 mmolL-1) was obtained at pH 6.95, temperature 37 0C and agitation 150 rpm. The results of this research can be used to explore the more potential microbial symbionts of the Macrotermes gilvus Hagen termite’s gut.
Moleculer Detection of Protozoa Trichodina spp. In Gourami (Osphromenus Gourame Lac.) Larvae with The infecting 18S rRNA Gene Marking in Exs. Residence of Banyumas, Central Java Rokhmani, Rokhmani; Setyawati, Endang Ariyani; Wahyono, Daniel Joko
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.11720

Abstract

Protozoa species of Trichodina spp. may be found in most hatchery centers in Banyumas, Purbalingga, and Banjarnegara. However, the determination of Trichodina spp. types is still based on its body’s morphological variations, not yet molecular. A research has been conducted to identify molekuler of the Trichodina spp. with the infecting 18S rRNA gene marking on the gourami larvae in Exs. Residence of Banyumas, Central Java. The research used a survey method with the samples of gourami. Amplification of 18S rRNA gene from Trichodina heterodentata was Performed using PCR technique. Primer used is Forward primer (5 ‘-AAC CTG GTT GAT CCT GCC ATG-3’) and Reverse primer (5 ‘-TGA TCC TTC TGC AGG TTC ACC TAC-3’) which produces a 600 pb amplicon of DNA. Molecular research can be a complementary identification of organisms morphologically. Amplification of the partial 18S rRNA gene may be used to identify Trichodina specifically. Gourami larvae taken from the hatchery centers in Banyumas, Purbalingga, and Banjarnegara. The results show that the detected percentage of Trichodina heterodentata genes with the infecting 18S rRNA gene marking on the gourami larvae in Central Java taken from the hatchery centers in Banyumas, Purbalingga and Banjarnegara are respectively 10%, 10%, and 45%. This research provides a benefit in mapping the presence of protozoa pathogen of Trichodina spp. in gourami hatcheries in the Former Exs. Residence of Banyumas, Central Java

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