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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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Articles 30 Documents
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Search results for , issue "Vol 8, No 1 (2016): March 2016" : 30 Documents clear
Bird Communities In Seblat Nature Recreation Park (SNRP) North Bengkulu, Bengkulu Susanto, Eki; Mulyani, Yeni Aryati; Suryobroto, Bambang
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4948

Abstract

Bird communities are the composition of several bird species that live together in the same place, time and interact with other birds. The diversity of birds in an area can be used as an indicator of stabilizing an area. Changes of vegetation structures due to logging practices can affect the availability of resources for bird communities. The objective of the research was to examine diversity, richness, bird species abundance and bird community similarity at HS1 (logged forest 1974), HS2 (logged forest 1989/1990) and HS3 (fully logged forest 1989/1990) in Seblat Nature Recreation Park (SNRP). The research was conducted in July September 2013. Data collection was conducted by point count method (total 36 points) and mist net method (total 4752 nets hours). The Research showed 85 birds species from 33 families were recorded. HS2 was highest value of diversity and richness (H = 3.63, DMG = 10.07). The highest relative abundance species in HS1 was Emerald Dove (Chalcophaps indica), while HS2 and HS3 were Slender-billed Crow (Corvus enca). The bird community similarity was highest in HS2 and HS3 (ISj = 0.58).How to CiteSusanto, E., Mulyani, Y. A., & Suryobroto, B. (2016). Bird Communities In Seblat Nature Recreation Park (SNRP) North Bengkulu, Bengkulu. Biosaintifika: Journal of Biology & Biology Education, 8(1), 25-32.
Mass Cultivation of Entomopathogenic Nematode in Artificial Media Indriyanti, Dyah Rini; Muharromah, Nur Lailatul
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5579

Abstract

Entomopathogenic nematodes Entomopathogenic nematodes (EPN) of the genera Heterorhabditis and Steinernema are commercially used to control pest insect. EPN is widely cultivated through in-vivo and in vitro methods. This research aims to discover the abundance of EPN cultivated in various artificial media. Seven types of media composition were tested in this research: media A (yeast + soybean powder), media B (yeast + chicken liver), media C (yeast + dog food), media D (yolk + soybean powder), media E (yolk + chicken liver), media F (yolk + dog food), and media G (yeast + yolk + dog food). Each media was inoculated in 1.2x103JI/mL. The growth of EPN was observed weekly in 4 weeks. Results showed that EPN could be cultivated using various media; media D, E, F, and G. Highest abundance of EPN is found in the second week of media D for 28164 JI/ml.Cell harvesting is suggested to be conducted during the first and second week to obtain maximum abundance of EPN.How to CiteIndriyanti, D., & Muharromah, N. (2016). Mass Cultivation of Entomopathogenic Nematode In Artificial Media. Biosaintifika: Journal of Biology & Biology Education, 8(1), 111-118.
Ethnobotanical Study of Rattans on Suku Anak Dalam Community in Bukit Duabelas Nasional Park Mairida, Dwi; Muhadiono, Muhadiono; Hilwan, Iwan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5164

Abstract

Suku Anak Dalam (SAD), a tribal in Bukit Duabelas Nasional Park (TNBD) has knowledge for utilizing rattans. This research attempts to investigate the species of rattans utilized and to analyze the local wisdom of Suku Anak Dalam(SAD) community.The data was collected by doing an interview the informants consisting of temenggung (head of the tribe), jenang (the liaison between the villagers and anak dalam), rattans gatherers, induk (mother) and hand maker. Then, the researcher observed active participation (following the SAD activity in utilizing rattans).The interview was conducted based on the appropriate time with qualitative condition and situation. The result showed 12 (twelve) species of rattan utilized by the SAD community, Calamus ornatus (Blume), Calamus caesius( Blume), Calamus flabellatus (Becc.), Calamus manan(Miq.), Calamus csipionum (Lour.), Calamus javensis(Blume), Calamus axilliaris(Becc.), Calamus sp., Daemonorops geniculata (Griff.) Mart., Daemonorops draco(Willd.) Blume, Daemonorops verticiliaris(Griff.) Mart., and Korthalsiaechinometra(Becc.)The eight species were utilized as ambung, penampilan, and tekalo handicraft. One type for rituals, ropes, clotheslines, dye, durian lempok cakes preservatives (Durio spp.),and the species for the food for pregnant mothers (ngidam), and the three species for medicine. The implication of this research gave a documentation for the government to formulate and to make an authority of TNB forest conservation and to protect the culture of SAD community in the form of rattans inventarization and local wisdom of SAD community in utilizing the species of rattans.How to CiteMairida, D., Muhadiono, M., & Hilwan, I. (2016). Etnobotanical Study of Rattans on Suku Anak Dalam Community in Bukit Duabelas Nasional Park. Biosaintifika: Journal of Biology & Biology Education, 8(1) 64-70.
Plant Species Composition and Their Conspecific Association in Natural Tropical Rainforest, South Papua Murdjoko, Agustinus; Marsono, Djoko; Sadono, Ronggo; Hadisusanto, Suwarno
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5217

Abstract

Papua has so many wide lowland areas that cover high diversity in plant from life-forms. In contrast, there is a lack of information concerning species diversity. The objectives of the research were to describe species of plant life-forms and described conspecific associations between small individuals and large individuals in natural tropical rainforest, south Papua. Then, 46 nested plots were placed systematically in natural tropical rainforest of Boven Digoel Regency, Papua. Density, frequency, dominance and importance value index (IVI) were analyzed to describe plant diversity, while detrended correspondence analysis (DCA) was to describe conspecific association between small individuals and large individuals. Results found 2040 individuals in this forest consisting 194 plant species. Those are from pteridophytes, angiosperms and gymnosperms. The plant life-forms are herbs, orchids, palms, pandans, shrubs, rattans, vines and trees in which of the plant life-forms, trees are the most abundant according to number of species. In the dominant plant species, two types of conspecific association occurred between small individuals and large individuals. Analysis of DCA revealed that three are large plant species grow along with their small individuals. Another type is that small individuals grow far from their large individuals.
Characterization of Carambola (Averrhoa carambola L.) Plant Collection of Cibinong Plant Germplasm Garden Based on Phenotypic and Genetic Characters Priadi, Dody; Perdani, Ambar Yuswi; Sulistyowati, Yuli; Pohan, Fiqolbi Nuro; Mulyaningsih, Enung Sri
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5199

Abstract

Indonesia as a rich biodiversity country has many superior fruit plant germplasms such as sweet star fruit or carambola (Averrhoa carambola L.). Some varieties of carambola which collected at the Germplasm Garden of Research Center for Biotechnology-LIPI have been used for parent trees of fruit plant production. Therefore, they have to be characterized both phenotypically and genetically. The objective of the study was to analyze the relationship between eight varieties of carambola i.e. Malaysia, Penang, Rawasari, Bangkok, Sembiring, Dewabaru, Demak and Dewimurni at the germplasm garden based on phenotypic and genetic characters. Phenotypic characters were observed directly in the field, whereas genetic characters were observed with RAPD markers using 10 primers. Phylogenetic analysis was done using NT-SYS software showed that there were three clusters of carambola varieties. Meanwhile, Malaysia and Penang varieties have closed relationships (96%) compared with the other varieties. The result of the study would be dedicated to updating and completing the existing fruit plant collection database of Plants Germplasm Garden.
Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Sugiharto, Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains ?-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 0.523 M and 1.456 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 97.96 M and 953.86 328.16 M respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1), 12-17.
Characteristics of superior soybean breeding lines tolerance to rust (Phakopsora pachyrhizi Syd.) Inayati, Alfi; Yusnawan, Eriyanto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5081

Abstract

Soybean rust caused by Phakopsora pachyrhizi is one of the most important diseases which limits soybean production. The aim of this study was to evaluate the resistance of 28 superior soybean lines and their tolerance to rust. The study was conducted at a screen house and arranged in a completely randomized design (CRD); three replications. All genotypes tested were artificially inoculated with P. pachyrhizi, and a set of un-inoculated genotypes was planted as a comparison. Number of pustules was recorded weekly, and resistant criteria was rated based on the International working group on soybean rust IWGSR method. Lesion color (LC), sporulation level (SL), number of uredia (NoU), frequency of pustule which had uredia, and yield were also recorded. Among 28 genotypes tested, only one was categorized as resistant and 2 genotypes were susceptible. Resistant genotypes had few pustules, lower AUDPC values, low disease severity, and Reddish Brown lesion type. Soybean rust affected yield components, i.e. number of intact pods and yield per plant. Yield loses due to rust in this study varied from 5-89%, and the average was 51%. The set of lines from Tanggamus pedigree showed more resistant to rust but less tolerant compared to Sinabung pedigree.How to CiteInayati, A., & Yusnawan, E. (2016). Characteristics of superior soybean breeding lines tolerancet to rust (Phakopsora pachyrhizi Syd.). Biosaintifika: Journal of Biology & Biology Education, 8(1), 47-55.
Effect of Turmeric Powder Supplementation to The Age of Sexual Maturity, Physical, and Chemical Quality of The First Japanese Quails (Coturnix japonica) Egg Saraswati, Tyas Rini; Tana, Silvana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4982

Abstract

The experiment was conducted to determine the effect of turmeric powder supplementation to the age of sexual maturity, physical, and chemical quality of the first Japanese quails (Coturnix japonica) egg. Forty five quails were assigned into a completely randomized design with three treatments (levels of turmeric powder, i.e., 0; 54; and 108 mg/quail/day) and each treatment used 15 quails. Turmeric powder supplementation was conducted before sexual maturity. Feed and drinking water provided ad libitum. Observed egg is an egg that was first produced. Parameters measured were the age of sexual maturity, feed intake, body weight, physical qualities which include: weight of egg, long axis, short axis, weight and thickness of shell, yolk index, Haugh unit, egg shell index. Whereas the observed chemical quality were cholesterol, HDL, LDL, protein, vitamin B12, vitamin A in eggs and egg shell calcium levels. The results showed that administration of turmeric powder can accelerate the age of maturity, increasing the levels of protein, HDL, vitamin A and B12 in eggs, decreasing the cholesterol and LDL content in eggs, but did not affect feed intake, physical quality of eggs and egg shell calcium levels. Based on the results of this study, it can be concluded that supplementation of turmeric powder improve the chemical quality of Japanese quail eggs (Coturnix japonica), so it is good for the development of quail embryos as well as for consumption.How to CiteSaraswati, T. R., & Tana, S. (2016). Effect of Turmeric Powder Supplementation To The Age of Sexual Maturity, Physical, and Chemical Quality of The First Japanese Quails (Coturnix japonica) Egg. Biosaintifika: Journal of Biology & Biology Education, 8(1), 18-24.
Antioxidant Activity from Various Tomato Processing Iswari, Retno Sri; Susanti, R
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4722

Abstract

Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the bodys nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, ?-carotene, vitamin C, ?-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. ?-tocopherol, ?-carotene and vitamin C) and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.
Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne & K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease Rahayu, Enni Suwarsi; Habibah, Noor Aini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5371

Abstract

Carica pubescens Lenne & K. Koch, an endemic species in Dieng mountains, must be conserved. The in vitro conservation has been developed, but sub-culture period needs to be extended. This study aimed to obtain a more efficient in vitro conservation protocol of C. pubescens. The research was carried out experimentally by using a completely randomized factorial design with three factors, namely decreasing in storage medium concentration (75% and 50% of MS medium), temperature (4oC and 8C), and irradiation duration (8 hours/day and 16 hours/day). Shoots were kept in the storage medium for 6, 9 and 12 months, then their viability were tested by growing them in the regeneration medium. Data were analyzed by Analyses of Variance and Least Significant Difference Test. The results showed that medium concentration of 50% of MS, the temperature of 8 C, and 16 hours/day of irradiation were able to suppress the C. pubescens growth in vitro storage for six months and could maintain its viability in the regeneration medium. Based on these results the medium concentration of 50% of MS, the temperature of 8 C and 16 hours/day irradiation can be used for in vitro conservation of C. pubescens without sub-culture for six months. How to CiteRahayu, E., & Habibah, N. (2016). Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne & K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease. Biosaintifika: Journal of Biology & Biology Education, 8(1), 85-93.

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