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All Journal HAYATI Journal of Biosciences Biosaintifika: Journal of Biology & Biology Education Journal of Public Health for Tropical and Coastal Region PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Majalah Biomorfologi (Biomorphology Journal) Journal of Multidisciplinary Applied Natural Science Sewagati
Manikya Pramudya
Faculty Of Science And Technology, Universitas Airlangga, Surabaya, Indonesia.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Sugiharto, Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains ?-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 0.523 M and 1.456 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 97.96 M and 953.86 328.16 M respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., & Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology & Biology Education, 8(1), 12-17.
Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Putri, Intan Permata; Savira, Nadyatul Ilma Indah; Winarni, Dwi; Suhargo, Listijani; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Intan Permata Putri; Nadyatul Ilma Indah Savira; Dwi Winarni; Listijani Suhargo; Win Darmanto
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Sugiharto Sugiharto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4969

Abstract

Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.How to CiteWahyuningsih, S., Pramudya, M., Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. Biosaintifika: Journal of Biology Biology Education, 8(1), 12-17.
A Preliminary Study of Ocimum basilicum Essential Oil as a Repellent against Aedes aegypti in Surabaya Wahyuni, Afrinda Dwi; Zahra, Siti Fatimatuz; Putri, Balqis Qonita; Pramudya, Manikya; Rohmah, Etik Ainun; Mulyatno, Kris Cahyo
Journal of Public Health for Tropical and Coastal Region Vol 5, No 2 (2022): Journal of Public Health for Tropical and Coastal Region
Publisher : Faculty of Public Health, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jphtcr.v5i2.13734

Abstract

Introduction: Aedes aegypti is the main dengue vector in Indonesia. One of the dengue controls is through insecticides use. The plant-based insecticide is one alternative that can be used to minimize the impact of chemical insecticides. Ocimum basilicum is one of the plants that have the potency to be used as insecticides. The aims of this study were to analyze the insecticide activity of Ocimum basilicum essential oil against Aedes aegypti as well as its Lethal Concentration (LC50 and LC90) and Knockdown Time ( KT50 and KT90).Methods: Treatment groups were given Ocimum basilicum essential oil of 100 ppm, 50 ppm, and 25 ppm. The tests were performed four times with 10 mosquitoes per test.Results: The mortality rate of the mosquito on the extract with a concentration of 100 ppm was 100%, 50 ppm was 10% and 25 ppm was 0% and the value of LC50 = 62.9 ppm and LC90 = 78.7 ppm. KT50 of this study was 19.4 minutes and KT90 was 8.07 minutes.Conclusion: Ocimum basilicum essential oil has a mosquitocidal effect on Aedes aegypti. Further studies to investigate the substances of Ocimum basilicum against Aedes aegypti are needed.
Pelatihan Pembuatan dan Pemberian Probiotik dan Single Cell Protein pada Sapi Perah di Komunitas Peternak Junrejo, Kota Batu Amin, Muhammad Hilman Fu'adil; Wibowo, Anjar Tri; Supriyanto, Agus; Pratiwi, Intan Ayu; Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Wahyuni, Dwi Kusuma; Junairiah, Junairiah; Dewi, Firli Rahmah Primula; Geraldi, Almando
Sewagati Vol 8 No 6 (2024)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v8i6.2340

Abstract

Komunitas peternak sapi perah di Junrejo, Batu menghadapi tantangan dalam meningkatkan produktivitas dan kualitas susu akibat rendahnya kesehatan ternak dan keterbatasan akses terhadap teknologi pakan yang efisien. Program pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman dan keterampilan peternak dalam memanfaatkan probiotik dan Single Cell Protein (SCP) sebagai pakan tambahan, yang diharapkan dapat meningkatkan kualitas kesehatan dan produktivitas ternak. Program ini menggunakan pendekatan partisipatif yang melibatkan pelatihan langsung dan sesi diskusi untuk memastikan penerapan yang efektif dan berkelanjutan. Probiotik dan SCP berperan dalam memperbaiki mikrobiota usus ternak, meningkatkan efisiensi pencernaan, dan memberikan sumber protein berkualitas tinggi. Hasil pelatihan menunjukkan bahwa setelah mengikuti kegiatan ini, seluruh peserta (100%) memahami dasar-dasar probiotik dan SCP, serta mampu menerapkannya secara mandiri di peternakan mereka. Di samping itu, penggunaan molase sebagai media perbanyakan probiotik mendukung prinsip ekonomi sirkuler, mengurangi limbah produksi gula, dan menciptakan solusi pakan yang ramah lingkungan. Program ini berhasil meningkatkan kapasitas komunitas peternak lokal, sehingga dapat berkontribusi terhadap kesejahteraan ekonomi mereka melalui peningkatan hasil susu dan biaya operasional yang lebih efisien.
Potential of Cinnamomum burmanni Leaf Extract as an Exogenous Antioxidant and Spermatoprotective for Rattus norvegicus L. Exposed to Polystyrene Nanoplastics Nurbani, Farah Annisa; Pramudya, Manikya; Safitri, Maya; Sugiharto; Aunurohim; Hayati, Alfiah; Septiani, Adiibtia; Dewi, Putri Alicia; Nashrulloh, Allam Thoriq; Vuanghao, Lim
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.693-699

Abstract

Polytyrene nanoplastics (NPs) (<1 µm) have high toxicity when entered and accumulated in cells. NPs accumulation causes oxidation stress, thus increasing reactive oxygen species (ROS) production, resulting in necrosis or apoptosis, as well as affecting endogenous antioxidant activity, such as superoxide dismutase (SOD) and catalase (CAT). Cinnamomum burmanni plant contains flavonoids, cinnamaldehyde, phen, and olic acid, potentially exogenous antioxidants. The study aims to analyze the potential of C. burmanni leaf extract for SOD and CAT levels, sperm quality, epithellia tubulus thickness, tubulus seminiferus diameter, and number of spermatogenic Rattus norvegicus cells exposed to NPs. Twenty-five male R. novergicus are divided into five groups (n = 5): two controls (without and with NPs) and three groups (combination of NPs and variations in extract concentrations of 100, 200, and 400 mg/kg. The SOD and CAT levels were measured with an ELISA kit. The histology was observed by counting the spermatogenic cells, measuring the epithellia thickness, and tubulus seminiferus diameter. The sperm motility, viability, and sperm count were observed to determine the sperm quality. Leaf extract of C. burmanni treatment with different concentrations not significantly increased SOD levels but significantly decreased the levels of CAT (P<0.05). The addition of C. burmanni leaf extract significantly increased the spermatogenic cell count, epithelia tubulous thickness, sperm viability, and sperm count (P<0.05), while sperm motility and tubulous seminiferous diameter not significantly increased. According to the results, C. burmanni leaf extract has antioxidant potential against the toxic effects of NPS.
Immunomodulatory Activity of Ganyong Tuber (Canna edulis K.) Extract on Phagocytosis, Leukocytes, and Antibody of Mice against Bacterial Infections Savira, Nadyatul Ilma Indah; Qulbi, Siti Shofa Assyifa; Marditianingsih, Meilia Putri; laili, Mumtahana; Narulita, Erlia; Pramudya, Manikya; Maulana, Alfan Irsyad
Majalah Biomorfologi Vol. 35 No. 2 (2025): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i2.2025.113-122

Abstract

Highlights Ganyong tuber exhibits immunomodulatory activity against Staphylococcus aureus infection in mice. Ganyong tuber extract increases phagocytic activity, leukocyte count, and spleen weight in infected mice.   ABSTRACT Background:  Ganyong tubers (Canna edulis K.) are widely consumed by Indonesians as an alternative food source. However, no studies have investigated the pharmacological properties of ganyong tubers, particularly their immunomodulatory activity. Objective: This study aimed to evaluate the immunomodulatory effects of ganyong tubers in mice infected with Staphylococcus aureus. Material and Method: This study used a randomized experimental design involving 30 male BALB/c mice divided into five groups, including a normal control, a negative control (infected with Staphylococcus aureus), and three groups treated with ganyong (Canna edulis K.) extract at doses of 50, 100, and 150 mg/kg BW. The extract was administered orally for seven days, followed by S. aureus injection. Phagocytic activity, leukocyte count, spleen weight, and antibody levels were evaluated using microscopy and ELISA. Data were analyzed using one-way ANOVA and Duncan’s post hoc test. Result: This study evaluated the immunomodulatory effects of ganyong tuber (Canna edulis K.) extract on phagocytosis activity and capacity, leukocyte count, spleen weight, and antibody levels in mice infected with Staphylococcus aureus. Phagocytosis activity and capacity were highest in the group receiving 50 mg/kg body weight (BW) extract, with decreasing effects at higher doses. Leukocyte counts were highest in the infected untreated group, while spleen weight was significantly increased in the 50 mg/kg BW treatment group compared to controls. Although the 100 mg/kg BW group showed the highest antibody levels, these differences were not statistically significant. Conclusion: Ganyong tuber extract demonstrated immunomodulatory activity by enhancing innate immune responses against S. aureus infection.
Peningkatan Kompetensi Guru Biologi SMA/MA Kabupaten Bojonegoro Melalui Pelatihan Genetika Molekuler: Improving the Competence of Biology Teachers at SMA/MA in Bojonegoro Regency Through Molecular Genetics Training Sugiharto, Sugiharto; Amin, Muhammad Hilman Fu'adil; Pramudya, Manikya; Hayati, Alfiah; Dewi, Firli Rahma Primula; Manuhara, Yosephine Sri Wulan; Soepriandono, Hari
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. Suppl-1 (2026): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v11iSuppl-1.11116

Abstract

Molecular Genetics has a positive impact on life. The SMA/MA curriculum has included this material as a mandatory subject for grade 12th. There are obstacles to its implementation due to teachers' lack of understanding and skills. The purpose of the community service program is to improve the competence of SMA/MA teachers in the molecular field, especially those who are members of MGMP Bojonegoro Regency. The activity was conducted on Saturday, August 30, 2025, at the Molecular Genetics Laboratory of the Biology Department, Airlangga University. A total of 25 participants attended this activity, which was coordinated by the Chair and Secretary of MGMP. Participants were given lectures and practicums on the technique of using micropipettes; isolating DNA and measuring its purity; and principles and materials for DNA amplification with PCR and electrophoresis. Referring to program results (1) an increase in highest score range during post-test (range 61-80 = 44%) compared to pre-test (range 21-40 = 60%), (2) highest score achieved by participants (pre-test = 70 is 4%, post-test = 90 is 20%) (3) percentage of increase in participants scores of more than 100% is 52%, (4) participants gave scores on satisfaction index above 95%, it can be concluded that this program can improve competence of Biology teachers at SMA/MA Bojonegoro Regency. This competency improvement is an effort to support the learning process and enhance the quality of education at the SMA/MA level, enabling students to excel in science.
Dual Antifungal and Immunoregulatory Actions of Topical Silver Nanoparticles from Piper ornatum Extract in Cutaneous Candida albicans Infection Dewi, Firli Rahmah Primula; `Mustofa, Laila Al Azizi; Hamdin, Candra Dwipayana; Geraldi, Almando; Lim, Vuanghao; Pramudya, Manikya; Hayati, Alfiah; Hajar, Versa Rachmania
Journal of Multidisciplinary Applied Natural Science Articles in Press
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.374

Abstract

Cutaneous Candida albicans infection is characterized by persistent inflammation, epidermal damage, and dysregulated immune responses. Silver nanoparticles (AgNPs) have emerged as promising antifungal agents with additional immunomodulatory properties; however, their effects on skin pathology and local immune responses during candidiasis remain incompletely defined. This study aimed to investigate the therapeutic effects of a topical AgNP-based cream in a murine model of C. albicans–induced skin infection. AgNPs were green-synthesized using an aqueous leaf extract of Piper ornatum. A murine model of cutaneous C. albicans infection was established, and infected mice were treated topically with AgNP-based cream formulations at concentrations of 4% or 6%. Disease severity was assessed through macroscopic skin evaluation and histopathological analysis. Immune modulation was examined by flow cytometric analysis of CD4⁺ T-cell subsets expressing TNFα and IL-17, as well as CD11b⁺ myeloid cells expressing IL-6 and IL-10. Untreated infected mice exhibited severe cutaneous pathology, including persistent erythema, erosive lesions, epidermal hyperkeratosis, and acanthosis. These changes were accompanied by marked immune dysregulation, characterized by expansion of CD4⁺IL-17⁺ T-cells, suppression of TNFα-producing CD4⁺ T-cells, increased IL-6 expression, and reduced IL-10 production in CD11b⁺ myeloid cells. Topical AgNP treatment significantly ameliorated macroscopic and histological skin damage, restoring epidermal architecture toward normal. Immunologically, AgNP therapy attenuated pathological Th17 responses, reduced IL-6-producing myeloid cells, enhanced IL-10 expression, and maintained TNFα at controlled levels. Both AgNP concentrations were effective, with the 4% AgNP formulation showing slightly superior normalization of epidermal thickness and inflammatory markers. Overall, topical AgNP-based cream effectively alleviated cutaneous C. albicans infection by combining antifungal activity with coordinated immunomodulation of both adaptive and innate immune responses. By suppressing excessive IL-17– and IL-6–driven inflammation while promoting regulatory immune pathways, AgNP treatment supports tissue repair and immune homeostasis, highlighting its potential as a therapeutic strategy for cutaneous candidiasis.
Co-Authors Agus Supriyanto Alfiah Hayati Almando Geraldi Anjar Tri Wibowo Aunurohim Dewi, Firli Rahma Primula Dewi, Putri Alicia Dwi Kusuma Wahyuni Dwi Winarni Erlia Narulita Firli Rahmah Primula Dewi Hajar, Versa Rachmania Hamdin, Candra Dwipayana Hari Soepriandono Intan Ayu Pratiwi, Intan Ayu Intan Permata Putri Intan Permata Putri Junairiah Junairiah Kris Cahyo Mulyatno, Kris Cahyo laili, Mumtahana Lim, Vuanghao Listijani Suhargo, Listijani Marditianingsih, Meilia Putri Maulana, Alfan Irsyad Maya Safitri Muhammad Hilman Fu'adil Amin Nadyatul Ilma Indah Savira Nashrulloh, Allam Thoriq Nurbani, Farah Annisa Putri, Balqis Qonita Qulbi, Siti Shofa Assyifa Rohmah, Etik Ainun S. Sugiharto Septiani, Adiibtia Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sugiharto Sugiharto Vuanghao, Lim Wahyuni, Afrinda Dwi Win Darmanto Yosephine Sri Wulan Manuhara Zahra, Siti Fatimatuz `Mustofa, Laila Al Azizi