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THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE Satoto, Satoto; Sulistyowati, Yuli; Hartana, Alex; -Loedin, Inez H. Slamet
Indonesian Journal of Agricultural Science Vol 9, No 2 (2008): October 2008
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of fivetransgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. DataÂ resulted from PCR of F2 population were analysed using a Chi Square test.The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of geneticsources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer orÂ separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.

Parameter Genetik dan Seleksi Sorgum [Sorghum bicolor (L.) Moench] Populasi F4 Hasil Single Seed Descent (SSD) Sulistyowati, Yuli; koesoemaningtyas, Tri; Sopandie, Didy; Ardie, Sintho Wahyuning; Nugroho, Satya
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

The objective of this study were to obtain information about genetic parameters of agronomic characters of sorghum populations derived from Single Seed Descent (SSD) method, and to estimate selection differensial and also to select of F4 population that have a high yield and medium plant height . The experiment was carried out at Leuwikopo Farm, Darmaga, Bogor from January to April 2014. The genetic material were F4 population derived from SSD. The results showed that based on the skewness and kurtosis values, all of the agronomy characters observed in this study were poligenic controlled and influenced by additive gene action. The genetic coefficient of variability values for all parameters varied from intermediate to high in F4 population. Agronomic characters in F4 responded positively to selection because of high broad sense heritability estimates. Correlation analysis showed that plant height, number of leaves, stem diameter, panicle length, panicle weight and 100 seed weight have significant and positive correlation to grain yield/plant. Differential value with selection intensity 10 % based on grain yield/plant will increase grain yield/plant 83.89 % and plant height 8.91 % in the next generation, whereas differensial value based on grain yield/plant and plant height will increase grain yield/plants 68.33 % and plant height 0.26 % in the next generation. It means that selected plant were expected increasing yield 83.89 % or 68.33 % in next generation.Keywords: heritability, cefficient genetic variability, correlation analysis, selection differential

Characterization of Carambola (Averrhoa carambola L.) Plant Collection of Cibinong Plant Germplasm Garden Based on Phenotypic and Genetic Characters Priadi, Dody; Perdani, Ambar Yuswi; Sulistyowati, Yuli; Pohan, Fiqolbi Nuro; Mulyaningsih, Enung Sri
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5199

Indonesia as a rich biodiversity country has many superior fruit plant germplasms such as sweet star fruit or carambola (Averrhoa carambola L.). Some varieties of carambola which collected at the Germplasm Garden of Research Center for Biotechnology-LIPI have been used for parent trees of fruit plant production. Therefore, they have to be characterized both phenotypically and genetically. The objective of the study was to analyze the relationship between eight varieties of carambola i.e. Malaysia, Penang, Rawasari, Bangkok, Sembiring, Dewabaru, Demak and Dewimurni at the germplasm garden based on phenotypic and genetic characters. Phenotypic characters were observed directly in the field, whereas genetic characters were observed with RAPD markers using 10 primers. Phylogenetic analysis was done using NT-SYS software showed that there were three clusters of carambola varieties. Meanwhile, Malaysia and Penang varieties have closed relationships (96%) compared with the other varieties. The result of the study would be dedicated to updating and completing the existing fruit plant collection database of Plants Germplasm Garden.

Genetic transformation of rice cv. Ciherang using double T-DNA vector harboring cry1Ab gene Sulistyowati, Yuli; Rachmat, Agus; Zahra, Fatimah; Rahmawati, Syamsidah; Nugroho, Satya
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Rice stem borer (Scirpophaga sp.) is considered as one of the primary pests causing yield decrease in Indonesia. Genetic engineering is considered as one of the most effective way to improve the resistance of rice plants to yellow stem borer since no resistance gene has been found in rice and its wild relatives. A double T-DNA binary vector p2TDNAcryIAb wich carry two independent T-DNA was used to transform Indonesian elite rice cv Ciherang using Agrobacterium-mediated transformation to generate transgenic rice that free from selectable marker genes. One T-DNA containing a selectable marker hygromycin phosphotransferase (hpt) gene and the other carrying the cry1Ab gene. As many as 37 putative transgenic plants from 14 embryos were generated. PCR analysis demonstrated that 22 out of 37 putative transgenic rice contain cry1Ab gene. All Ciherang rice carrying the cry1Ab gene expressing Cry1Ab protein as shown by the immunostrip assay. Further work will be carried out to determine the transgenes copy number, to select marker free transgenic rice, and to evaluate the resistance of transgenic rice against neonates larvae of yellow stem borer.Keyword: double T-DNA, transgenic rice, Cry1Ab, transformation

Segregation Analysis of Transgenic Rice Plants CV Rojolele Harboring Cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Estiati, Amy; Sulistyowati, Yuli; Zahra, Fatimah; Nurhasanah, Ade Nena; Rahmawati, Syamsidah; S-Loedin, Inez H
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous forÂ cry1B gene. SelectionÂ of homozygous transgenic rice lines containingÂ cry1BÂ gene at third, fourth and fifth generation had been conducted by PCR.Â The presence ofÂ cry1BÂ gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic riceÂ lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines forÂ cry1BÂ gene. Â Â Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed thatÂ these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy ofÂ cry 1BÂ gene to yellow stem borer in naturalcondition, confined field trial in endemic area of yellow stem borer should be conducted.Â Keywords: bioassay, cry1B gene, transgenic rice, yellow stem borer

PARAMETER GENETIK DAN SELEKSI SORGUM [SORGHUM BICOLOR (L.) MOENCH] POPULASI F4 HASIL SINGLE SEED DESCENT (SSD) Sulistyowati, Yuli; koesoemaningtyas, Tri; Sopandie, Didy; Ardie, Sintho Wahyuning; Nugroho, Satya
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2878

The objective of this study were to obtain information about genetic parameters of agronomic characters of sorghum populations derived from Single Seed Descent (SSD) method, and to estimate selection differensial and also to select of F4 population that have a high yield and medium plant height . The experiment was carried out at Leuwikopo Farm, Darmaga, Bogor from January to April 2014. The genetic material were F4 population derived from SSD. The results showed that based on the skewness and kurtosis values, all of the agronomy characters observed in this study were poligenic controlled and influenced by additive gene action. The genetic coefficient of variability values for all parameters varied from intermediate to high in F4 population. Agronomic characters in F4 responded positively to selection because of high broad sense heritability estimates. Correlation analysis showed that plant height, number of leaves, stem diameter, panicle length, panicle weight and 100 seed weight have significant and positive correlation to grain yield/plant. Differential value with selection intensity 10 % based on grain yield/plant will increase grain yield/plant 83.89 % and plant height 8.91 % in the next generation, whereas differensial value based on grain yield/plant and plant height will increase grain yield/plants 68.33 % and plant height 0.26 % in the next generation. It means that selected plant were expected increasing yield 83.89 % or 68.33 % in next generation.Keywords: heritability, cefficient genetic variability, correlation analysis, selection differential

RESPONSE OF SIX CHILI VARIETIES TO ANTHRACNOSE DISEASE CAUSED BY Colletotrichum acutatum AND C. gloeosporioides Perdani, Ambar Yuswi; Paradisa, Yashanti Berlinda; Wahyuni, Wahyuni; Indrayani, Sri; Sulistyowati, Yuli; Cahyani, Yani
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221144-150

Response of six chili varieties to anthracnose disease caused by Colletotrichum acutatum and C. gloeosporioides. Chili is one of the horticultural commodities with high economic value. Chili production is constrained by anthracnose diseases. Losses due to anthracnose can reduce the fruits quality and yields lose. This study aims to determine the resistance of several chili varieties to anthracnose. Genetic material was used six varieties of chili. Ripe chilies were inoculated with two types of Colletotrichum isolates, i.e. C. acutatum and C. gleosporides. The experiment was arranged in a factorial randomized block design with three replications. The first factor were chili varieties: Laris, SSP, Habanero, Cibinong, Ekasari, and Kopay. The second factor was two types of fungal isolates. Observations were made on the incidence and severity of disease due to anthracnose. The results showed that both fungal isolates were effective in causing anthracnose disease in chilies. Habanero was very susceptible to anthracnose. Laris and Ekasari were moderately resistant to anthracnose diseases. These findings are important to develop new Capsicum cultivars that are more adaptive to anthracnose disease.

IMPLEMENTATION OF EPANET TECHNOLOGY IN THE DESIGN OF A CLEAN WATER SYSTEM IN DAMARKASIAN VILLAGE Faqih, Nasyiin; Herlambang, Sigit; Sulistyowati, Yuli; Roehman, Fatchur; Mushthofa, Mushthofa
Jurnal Disprotek Vol 16, No 1 (2025)
Publisher : Universitas Islam Nahdlatul Ulama Jepara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34001/jdpt.v16i1.7166

Water is a fundamental necessity that must be utilized wisely. This study was conducted in Damarkasian Village, located at the foot of Mount Sindoro and characterized by its hilly terrain. The village has a very limited supply of clean water, forcing residents to rely on a neighboring village for their water needs. Although there is a water distribution system in place, only wealthier residents can afford to build their own networks, while those less fortunate must rely on public facilities along the roadside. To address the uneven water distribution, a well-planned clean water distribution system based on gravity flow is essential. Field data collected includes spring water discharge, water quality, distance, and elevation, as well as population data from official statistics. Water discharge was measured using the volumetric method, and elevation and distance were recorded using the Q-GIS application. Network evaluation, conducted using EPANET 2.0 software, indicated a water discharge rate of 12.2 liters per second. To meet the village's water needs through 2037, a discharge rate of 10.57 liters per second is required. A total of 39 service tanks and appropriately sized PVC pipes are planned without the need for pumps, reducing costs. The total budget is estimated at IDR 1.4 billion, with a tariff of IDR 1,300/m³, cheaper than the PDAM rate.PENERAPAN TEKNOLOGI EPANET DALAM RANCANGAN SISTEM AIR BERSIH DI DESA DAMARKASIANAir adalah kebutuhan mendasar yang harus dimanfaatkan secara bijak. Penelitian ini dilakukan di Desa Damarkasian, yang terletak di kaki Gunung Sindoro dan memiliki kontur perbukitan. Sumber air bersih di desa ini sangat terbatas, sehingga penduduk bergantung pada desa tetangga untuk pasokan air. Meskipun ada distribusi air, hanya masyarakat yang mampu yang dapat membangun jaringan sendiri, sementara warga kurang mampu menggunakan fasilitas umum di pinggir jalan. Untuk mengatasi masalah distribusi air yang belum merata, diperlukan perencanaan sistem distribusi air bersih dengan sistem gravitasi. Data lapangan yang dikumpulkan mencakup debit mata air, kualitas air, jarak, dan elevasi, serta data penduduk dari badan statistik. Debit air diukur dengan metode volumetrik, dan elevasi serta jarak dengan aplikasi Q-GIS. Evaluasi jaringan menggunakan software EPANET 2.0 menunjukkan debit air sebesar 12,2 lt/detik. Untuk memenuhi kebutuhan penduduk hingga 2037, diperlukan debit 10,57 lt/detik. Sebanyak 39 bak pelayanan dan pipa PVC dengan diameter yang sesuai direncanakan tanpa pompa, yang menghemat biaya. Total anggaran sebesar Rp. 1,4 miliar, dengan tarif Rp. 1.300/m3, lebih murah dari PDAM.

GOOD HEALTH: SALAK FLAVORED COFFEE DRINK VARIANT AS A MEANS OF MENTAL HEALTH EDUCATION FOR THE YOUNGER GENERATION Sulistyowati, Yuli; Rizky Ramadhon, Ahmad; Albaz Ulumudin, Muhammad; Eryanto, Aji
CATHA SAINTIFICA Vol 2 No 2 (2024): November 2024
Publisher : Sentra Kekayaan Intelektual dan Inovasi Teknologi (INOTEK) Universitas Sains Al-Qur'an

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/cathasaintifica.v2i2.8810

Sukoharjo is an area in Wonosobo Regency that has the potential as a salak pondoh-producing area. The potential of salak pondoh which is quite good in this area can provide new business opportunities that can improve the economy. A new innovation of processed salak pondoh which can be used as salak powder can be used for aroma and taste to create new variations in processed beverage products, including coffee beverage products. Coffee drinks are a type of drink that is quite in high demand among the younger generation. A variety of coffee drinks combined with salak powder is a new type of coffee drink variant that can provide its own attraction. This reason is the basis for using salak flavored coffee drinks as a means to provide mental health education through the packaging used. This is because mental health education is very necessary among the younger generation to prevent the onset of mental disorders. This salak flavored coffee drink variant product is called Good Health. The way to make Good Health products is starting from the preparation of tools and materials, the manufacture of salak powder, to the manufacture of beverages, packaging, and product storage. In addition to having advantages in the distinctive taste and aroma of salak fruit, this coffee drink also has advantages in the form of unique packaging with quotes in the form of inspirational words that educate mental health

GOOD HEALTH: SALAK FLAVORED COFFEE DRINK VARIANT AS A MEANS OF MENTAL HEALTH EDUCATION FOR THE YOUNGER GENERATION Sulistyowati, Yuli; Rizky Ramadhon, Ahmad; Albaz Ulumudin, Muhammad; Eryanto, Aji
CATHA SAINTIFICA Vol 2 No 2 (2024): November 2024
Publisher : Sentra Kekayaan Intelektual dan Inovasi Teknologi (INOTEK) Universitas Sains Al-Qur'an

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/cathasaintifica.v2i2.8810

Sukoharjo is an area in Wonosobo Regency that has the potential as a salak pondoh-producing area. The potential of salak pondoh which is quite good in this area can provide new business opportunities that can improve the economy. A new innovation of processed salak pondoh which can be used as salak powder can be used for aroma and taste to create new variations in processed beverage products, including coffee beverage products. Coffee drinks are a type of drink that is quite in high demand among the younger generation. A variety of coffee drinks combined with salak powder is a new type of coffee drink variant that can provide its own attraction. This reason is the basis for using salak flavored coffee drinks as a means to provide mental health education through the packaging used. This is because mental health education is very necessary among the younger generation to prevent the onset of mental disorders. This salak flavored coffee drink variant product is called Good Health. The way to make Good Health products is starting from the preparation of tools and materials, the manufacture of salak powder, to the manufacture of beverages, packaging, and product storage. In addition to having advantages in the distinctive taste and aroma of salak fruit, this coffee drink also has advantages in the form of unique packaging with quotes in the form of inspirational words that educate mental health

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