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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Analisis Sebagian Sekuen Gen Ferritin2 pada Padi (Oryza sativa L.) Indragiri Hilir, Riau Fadel Nugraha; Dewi Indriyani Roslim; Yolla Putri Ardilla; Herman -
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3102

Abstract

Ion Fe bebas sangat beracun bagi tanaman, karena dapat membentuk radikal bebas di dalam sel.  Walaupun demikian, tanaman memiliki mekanisme untuk mempertahankan homeostasis Fe di dalam sel yang melibatkan protein ferritin.  Penelitian ini bertujuan untuk menganalisis dan membandingkan sekuen nukleotida gen ferritin2 dari dua genotipe padi lokal (Bakung dan Serei) dari Indragiri Hilir, Riau dengan varietas padi rawa unggul tahan cekaman kelebihan Fe (Siam Sintanur), padi unggul tidak tahan cekaman kelebihan Fe (IR64), dan data sekuen nukleotida kultivar Nipponbare.  Metode penelitian meliputi isolasi DNA toal dari daun segar tanaman padi menggunakan metode CTAB dan amplifikasi DNA (PCR) menggunakan primer OsFer_F3 (forward) dan Gross_R (reverse).  Produk PCR kemudian disekuensing dan disejajarkan.  Pada penelitian ini telah diperoleh fragmen dari gen ferritin2 dari kelima genotype atau varietas padi yang diuji, yang berukuran sekitar 1200 pb.  Analisis pensejajaran menunjukkan terdapat 56 SNP (single nucleotide polymorphism) pada sekuen nukleotida gen ferritin2 tersebut.  Bakung menunjukkan kedekatan yang tinggi dengan Nipponbare, diikuti dengan IR64, Siam Sintanur, dan Serei.  Kemungkinan Bakung merupakan genotipe padi lokal dari Indragiri Hilir, Riau yang tahan cekaman kelebihan Fe. Free Fe ions are highly toxic to plants, because it can form free radicals in the cells.  However, plants have mechanisms to maintain Fe homeostasis in the cells involving ferritin proteins.  This study was aimed to analyze and to compare the nucleotide sequence of ferritin2 gene in two local rice genotypes (namely Bakung and Serei) from Indragiri Hilir, Riau and in Fe overload-tolerant rice variety (Siam Sintanur), Fe overload-sensitive rice variety (IR64), as well as the nucleotide sequence of Nipponbare rice cultivar.  The research methods consisted of DNA isolation from fresh leaves of rice plants using CTAB method and DNA amplification (PCR) using a couple of primers, OsFer_F3 (forward) and Gross_R (reverse).  The PCR products were then sequenced and aligned.  DNA fragments of ferritin2 gene with length of approx. 1200 bp were obtained from those four rice varieties or genotypes tested.  Alignment revealed 56 SNPs (single nucleotide polymorphisms) in the ferritin2 gene sequences.  Bakung showed close distance with Nipponbare, followed by IR64, Siam Sintanur, and Serei.  It wa suggested that Bakung was Fe overload-tolerant local rice genotype from Indragiri Hilir, Riau.
Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent Risa Fitriandini; Sri Budiarti; Yulin Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10323

Abstract

Rhododendron has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in ISP2 medium.  The antibacterial activity was assayed against Enteropathogenic Escerichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus.  The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e.  RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively.  The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 oC. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one.  From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent. 
In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD Triono Bagus Saputro; Fathin Finariyah; Siti Dianawati; Nur Fadlilatus Sholihah; Dini Ermavitalini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6934

Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2.4 D. The result show that 4 ppm of 2.4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contain NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66.7%. Furthermore, Manding variety shows a better performance in callus weight improvement with 170 mg, while Talango shows no improvement of callus weight. The result of RAPD analysis indicate that the genome characteristic were different between initial callus and surviving callus. Out of ten primers, only five primers shows polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. Manding can be further developed as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.How to CiteSaputro, T. B., Finariyah, F., Dianawati, S., Sholihah, N. F., Ermavitalini, D. (2016). In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD. Biosaintifika: Journal of Biology Biology Education, 8(3), 344-352. 
Conservation Status of Agarwood-Producing Species (Gyrinops versteegii) in Indonesia Sutomo Sutomo; Rajif Iryadi; I Made Sumerta
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.27809

Abstract

Aquilaria malaccensis and Gyrinops versteegii are agarwood producing plant species that is widely used because of its fragrance. Gyrinops versteegii has not been much cultivated and along with the decreasing population of G. versteegii in its natural habitat. This study aimed to assess scarcity status of Gyrinops versteegii based on distribution records from both herbarium and field exploration to assist the formulation of its conservation policy. Distribution data were obtained from online database and also from field exploration in Lombok, Sumbawa, and Flores Islands to obtain the population information. Area of Occupancy (AOO) and Extent of Occurrence (EOO) were calculated using GeoCAT (Geospatial Conservation Assessment Tool) and IUCN status recommendation was discussed. The estimated EOO was 868,422,919 km2, exceeding the value required for the threatened category. Based on EOO, it is included in the Least Concern (LC) category, but the EOO covers a large area of the ocean so the AOO was 116 km2 as meets criterion B (AOO500 km2). It can be categorized into endangered (EN). Population data and conservation status of G verstegii are very important to provide recommendations on the quota wild-harvesting of agarwood by stakeholders.
Anthracnose Disease on Vegetables Crops in Serang Village, District of Karangreja, Purbalingga Regency Eddy Tri Sucianto; Muachiroh Abbas; Endang Sri Purwati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21818

Abstract

Various vegetables are widely cultivated in Serang Village, District of Karangreja, Purbalingga Regency. This village is located at 1350 above sea level. Vegetable productions in Serang are often constrained by the presence of diseases caused by pathogenic fungi Colletotrichum sp. called anthracnose. However, there was no data regarding anthracnose attack in vegetable crops in Serang. This study aimed to find out the types of vegetable that were attacked by anthracnose disease as well as the intensity of the attack in a vegetable crops in Serang Village, Karangreja District, Purbalingga Regency. Vegetables with anthracnose were purposively selected from 10 different locations. The anthracnose disease was identified based on their symptoms and analyzed descriptively. The results showed that five different vegetable crops suffer from anthracnose, and the intensity of anthracnose ranges from 32% up to 52%. Those intensity percentages indicate that large part of vegetable crops in Serang Village was attacked by anthracnose and possibly cause a significant decrease in vegetable production in that village. Our results provide the first scientific data about anthracnose attack in Serang Village. The data is essential for the management of vegetable crops in Serang Village.
Practical Guide Based on Learning Cycle 5E to Enhance Students’ Cognitive Learning Outcomes Dwi Safriani Pangestika; Zuhdan Kun Prasetyo
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.14884

Abstract

The practical guide is a teaching material that students use in labwork activities. This learning cycle 5E (Engangement, Exploration, Explanation, Elaboration, Evaluation) on practical guide is expected to be used as an alternative choice for teacher in improving students’ cognitive learning outcomes in concept of pressure and symptoms of capillarity in plants. The aim of this study was : 1) to describe the feasibility of develop practical guide based on learning cycle 5E and 2) to analyse the improvement of the practical guide based on learning cycle 5E to enhance cognitive learning outcomes of eight graders of junior high school in science lesson. Here, we used the Research and Development refers to the procedural steps of the 4D models by Thiagarajan. To test the efficacy, pretest and posttest were employed.This is a quasi-experiment research conducted in MTsN 3 Ngawi. Research subjects were all students of grade 8 by taking class 8A as the experimental class and 8B as the control class. Samples were taken by cluster sampling technique. The result of the feasibility test showed that practical guide included in the excellent category. T-test analysis suggests that the practical guide based on learning cycle 5E could improve students’ cognitive learning outcomes significantly. In addition to contributing to the improvement of students’ cognitive learning outcomes in MTsn 3 ngawi, the development of science cycle-based learning guide based on learning cycle 5e can also provide students practice skills in learning so that it is expected to contribute posotif in the community.
Parasitoid Hama Penggerek Batang dan Pucuk Tebu di Cinta Manis, Ogan Ilir Sumatera Selatan Dewi Meidalima
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2928

Abstract

Serangan hama penggerek  tebu siap panen sebesar 11,25% di Cinta Manis tahun 2007. Lahan pengamatan 1 ha di bagi menjadi 5 petak secara diagonal, masing-masing seluas 200 m2, populasi tanaman sebanyak 1500 batang. Tujuan penelitian mengetahui keanekaragaman, spesies parasitoid dan potensi parasitasinya. Ditemukan 3 spesies parasitoid telur (Trichogramma chilonis; Telenomus dignoides; Tetrastichus schoenobii) dan 3  spesies parasitoid larva (Rachonathus scirpophagae; Stenobracon nicevillei; Diatraeophaga striatalis).  Parasitisasi parasitoid telur penggerek pucuk di lahan dengan dan tanpa tumbuhan liar oleh Telenomus dignoides, Tetrastichus schoenobii, Trichogramma chilonis sebesar 6,96% dan 80,48%; 29,13% dan 15,22%; 0% dan 0,71%.   Parasitisasi parasitoid telur penggerek batang di lahan dengan dan tanpa tumbuhan liar oleh Tetrastichus schoenobii sebesar 48,88% dan 36,08%, Telenomus dignoides sebesar 29,35% dan 28,34%. Parasitisasi parasitoid larva penggerek pucuk di lahan dengan dan tanpa tumbuhan liar oleh Rachonothus scirpophagae sebesar 2,66% dan 1,59%, dan Stenobracon nicevillei sebesar  1,81% dan 0,99%.  Parasitisasi parasitoid larva penggerek batang (Diatraeophaga striatalis), di lahan dengan dan tanpa tumbuhan liar sebesar 32,23% dan 19,62%. Keanekaragaman spesies parasitoid telur (H’=0,627)dan larva penggerek pucuk (H’=0,686) tertinggi pada lahan dengan tumbuhan liar. Keanekaragaman spesies parasitoid telur penggerek batang tertinggi pada lahan tanpa tumbuhan liar adalah H’=0,686. During 2007 in Cinta Manis area, the attack of borers on readily harvested sugarcane reached 11.25%. A 1-ha observation field was divided diagonally into 5 plots, each with an area of 200 m2, and populated with 1,500 plants. The aims of this study were to know the diversity of parasitoids species and their parasitic potentials. As many as 3 species of parasitoid eggs (Trichogramma chilonis; Telenomus dignoides; Tetrastichus schoenobii) and 3 species of parasitoid larvae (Rachonathus scirpophagae; Stenobracon nicevillei; Diatraeophaga striatalis) have been found. The parasitization of shoot borer egg parasitoids (Telenomus dignoides, Tetrastichus schoenobii, Trichogramma chilonis) in the field with and without wild plants were 61.96% and 80.48%; 29.13% and 15.22%; 0% and 0.71%, respectively. The parasitization of stem borer egg parasitoids of Tetrastichus schoenobii in the field with and without wild plants were 48.88% and 36.08%, respectively, and of Telenomus dignoides were 29.35% and 28.34%, respectively. The parasitization of shoots borer larval parasitoids of Rachonothus scirpophagae in the field with and without wild plants were 2.66% and 1.59%, respectively, and of Stenobracon nicevillei were 1.81% and 0.99%, respectively. Parasitization of larvae parasitoid stem borer (Diatraeophaga striatalis) in the field with and without the wild plants were 32.23% and 19.62%, respectively. The highest diversity of eggs (H’=0.627) and larvae (H’=0.686) of shoot borer parasitoid species were in the field with wild plants. The highest diversity of egg stem borer parasitoid species in the field without wild plants was H’=0.686.
Exploration of Mango Fruits (Mangifera indica) as α-Glucosidase Inhibitors Nadia Prilliane Putri; Khalida Shabiba Nursyamsi; Yanico Hadi Prayogo; Dina Ragillia Sari; Eka Budiarti; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10516

Abstract

Mango fruit (Mangifera indica L.) is the tropical fruit that grows easily in Indonesia with plenty varieties. This study aimed to determine the varieties of mango fruit and the most potent part of mango as antidiabetic agent through α-glucosidase inhibitory activities. Four types of mango fruit (indramayu, manalagi, harum manis, and budiraja) were used in this study. Each part of the mango fruit:peel, flesh, endosperm, and endocarp were extracted by maceration process with three different solvents (n-hexane, ethyl acetate (EtOAc), and ethanol (EtOH)). An ability of all 46 extracts in inhibiting the α-glucosidase at a concentration of 500 ppm were determined. Then 11 extracts with the high inhibition value were determined their IC50 (concentration to inhibit 50% activity) values. EtOAc extract of manalagi, indramayu, and budi raja endosperm had the lowest IC50 value which was not statistically significantly different (at 95%) with EtOAc extract of budi raja peel. The bioautographic Thin Layer Chromatogram showed that the most active band is characterized by white luminescence under UV 366 nm, yellow color under UV 254 and visible light. The band with Rf 0.93 from EtOAc endosperm extract of indramayu and manalagi and Rf 0.73 from EtOAc budi raja peel extract are the most active band which predicted as a flavonoid. The result adds the value of the peel and seed of mango, as well as an alternative in blood sugar control, which is easy to obtain, relatively cheap, and liked by the community. 
A Dermatoglyphic Study: Association of Fingerprint Patterns Among ABO Blood Groups Tri Panjiasih Susmiarsih; M. Samsul Mustofa; Mirfat Mirfat
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5189

Abstract

Fingerprints are probably the most common biometric technique used in personal identification. The potential of fingerprints to determine sex and human identification has been well exhibited. However, very few studies have been conducted correlating finger prints with blood groups. The aim of this study was to investigate the distribution of fingerprint patterns based on ABO blood groups. The total sample consisted of 302 medical students of YARSI University Jakarta comprising of 187 females and 115 males. The fingerprint patterns were classified into arches, loops (ulnar and radial), whorls. counted and comprised triradius and total ridge count. The data analysis used Chi Square and Student-T test.The study results indicated that there were fourth especially pattern type. Significantly (p0.05), frequency of loop types (60.36%) was highest in B blood, whorl type was highest in O blood (40.45%) and arches in AB blood was higher (5.12%) as compared to other groups. Dankmeijer indices of O and AB blood were 3.78 and 11.34, respectively. There were indicated significantly (p0.05) difference of average ridge count total among ABO blood groups. This study implied an association between dermatoglyphics and blood groups.How to CiteSusmiarsih, T. P., Mustofa, M. S., Mirfat, M. (2016). A Dermatoglyphic Study: Association of Fingerprint Patterns Among ABO Blood Groups. Biosaintifika: Journal of Biology Biology Education, 8(3), 294-300. 
Comparison of Nutritional Content of Mangifera indica L. var. Wirasangka with Three Other Varieties in Tegal Regency Enni Suwarsi Rahayu; Amin Retnoningsih; Muhammad Abdullah; Priyanti Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26728

Abstract

Mangifera indica L. var. wirasangka or wirasangka mango, a local plant in Tegal Regency, has a low population because it is rarely planted by people as its unknown nutritional content. To increase people's interest in planting, it is necessary to know the nutritional content of wirasangka mango. The aim of the study was to compare the nutritional content of wirasangka mango with three other varieties in Tegal Regency. This study used a one-factor completely randomized design. The factor was the mango variety which included six varieties, namely the varieties of wirasangka from three areas, okyong, tengkoeh, and golek. The variables observed were content of water, fiber, fructose, β-carotene, potassium and iron, as well as vitamin C and antioxidant activity. Normally distributed data were analyzed using One-Way Analysis of Variance and Least Significant Difference (LSD) test. Besides, the non-normally distribution data were analyzed using Kruskal Walis and continued with post-hoc test, i.e. Mann-Whitney. The results showed that the levels of water, dietary fiber, fructose, β-carotene, and iron were significantly different at the significance level of 0.1. Wirasangka mango has a higher fiber, and fructose content, but lower of β-carotene and iron content compared to okyong, tengkoeh and golek mangoes. The levels of vitamin C, potassium, and antioxidant activity of wirasangka mango were equivalent to the three other varieties studied. Thus the wirasangka mango has advantages which are expected to increase the interest of people in Tegal Regency to plant this variety. As a result, its population in the future will also increase.

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