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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Bryophytes in Cibodas Botanical Garden: Diversity and Potential Uses Ainun Nadhifah; Madinatul Khujjah; Pantalea Edelweiss Vitara; Ikhsan Noviady
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14433

Abstract

Research on the diversity of bryophytes in Indonesia has been generally accomplished. However, the publication of potential uses of this tiny plant is very limited. Cibodas Botanical Garden (CBG) as a center for ex-situ plant conservation has potential climate conditions as a location of moss growth. The study aims to collect bryophytes in CBG and to identify their potential uses. Purposive sampling method was used on various substrate (soil, rocks, trees). Some environmental parameters were also recorded, for instance, substrate type, temperature, and humidity. All of bryophytes collections were taken to the laboratory for the identification and saved in herbarium (CHTJ). A literature study was conducted to determine the potential uses of identified moss species. From 153 collection numbers recorded in CBG, there were 42 species which have potential use. In addition, there were 42 species that have the potential to be used as a medicinal plant, ornamental plants, uses in agriculture and environmental services. This study provides the initial information about the potential uses of mosses, liverworts, and hornworts particularly in Indonesia. Furthermore, these prospective utilization encourage the innovative research in Indonesia, particularly on alternative natural resources.
Identifikasi Rhizoctonia Mikoriza Pada Anggrekan Dan Kelompok Anastomosisnya Haryuni -
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.2573

Abstract

Penelitian ini dilakukan di Laboratorium Klinik Fakultas Pertanian Universitas Gadjah Mada Yogyakarta dan Pusat Penelitian di Laboratorium Biologi Fakultas Pertanian Universitas Gifu di Jepang.Tujuan penelitian ini untuk mengidentifikasi dan melakukan anastomosis isolat jamur Rhizoctonia mikoriza (TMG-2, SR-9 dan SR-8). Tester yang digunakan yaitu AG-F SIR.9, AG-F Fko.2.28, and AG-F PS.17. Hasil penelitian menunjukkan bahwa Identifikasi SR-8 memiliki ciri pada Rhizoctonia binukleat (BNR) dan dikelompokkan kedalam AG-F (teleomorf: Ceratobasidium sp.)The experiment was carried out at the Laboratory of Clinical Plant Pathology, Faculty of Agriculture, Gadjah Mada University in Yogyakarta and the Research Center at the Laboratory of Agriculture Biology, Gifu University in Japan. The objectives of the experiment were to identify and to test anastomosis group of orchid mycorrhizal Rhizoctonia TMG-2, SR-9, and SR-8 isolates. The tester of Rhizoctonia to be used were AG-F SIR.9, AG-F Fko.2.28, and AG-F PS.17. Results of the study showed that SR-8 belongs to binucleate Rhizoctonia (BNR) and grouped into AG F (teleomorph: Ceratobasidium sp).
Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Sri Puji Astuti Wahyuningsih; Manikya Pramudya; Intan Permata Putri; Nadyatul Ilma Indah Savira; Dwi Winarni; Listijani Suhargo; Win Darmanto
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences Nia Kurniawaty; Purnama Hidayat; Aunu Rauf
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5448

Abstract

Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of  Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps  DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis.  PCR programme was succesfully to amplified mtCOI gene fragment at 710 bp. The length of mtCOI gene of Gynaikothrips uzeli, Haplothrips ganglbaueri, and Pseudophilothrips ichini were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mtCOI gene mitochondrial had successfully supported the morphological data. How to CiteKurniawaty, N., Hidayat, P. Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. Biosaintifika: Journal of Biology Biology Education, 8(2), 185-192.
Qualitative Tests of Secondary Metabolite Compounds in Ethanol Extract of Spirulina platensis from Karimun Jawa Sea, Indonesia Renni Yuniati; Muhammad Zainuri; Hermin Kusumaningrum
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23153

Abstract

Spirulina platensis is a spiral green-blue algae that is abundant in the subtropical and tropical water bodies. S. platensis has several potentials to be used in the medical fields due to its wound healing and antitumor potentials. This research aimed to evaluate the secondary metabolite compounds contained in the ethanolic extract of S. platensis from Karimun Jawa Sea, Indonesia. The dried S. platensis was macerated and extracted using ethanol, then was filtered using Whatman filter paper. A series of tests has been done to determine the secondary metabolites in the ozonated oil using several chemistry tests and reagents in the ethanol extract of S. platensis. The tests found that S. platensis microalgae harvested from Karimun Jawa sea contains alkaloid, saponin, flavonoid, and quinone. As S. platensis from different region contains different substances, this research shows the substances contained in S. platensis from Karimun Jawa sea. With this research, we knew that S. platensis from Karimun Jawa islands contains alkaloid, saponin, flavonoid, and quinone which can be used as a medicinal ingredient.
Habitat Suitability for Hoya spp. (Apocynaceae) in The Bodogol Conservation Area, West Java Masrayani Sulaeman; Sulistijorini Sulistijorini; Sri Rahayu
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.13021

Abstract

Hoya is a tropical flowering plant that has great potential as an export commodity. It needs to be cultivated to ensure its sustainability in nature. Information about the suitable habitat of Hoya was not widely known yet. This study aimed to compare the habitat characteristics of several species of Hoya. The research was conducted by exploring the sample plots determined by purposive sampling based on the differences of habitat characteristics. The biotic components observed were phorophyte species type and the number of individual of each Hoya species. The abiotic components measured in each plot were altitude and geographical position, air temperature, humidity, light intensity, wind speed, and canopy cover. Hoya ‘s diversity was analyzed using the Shannon Winner Index. Environmental factor data and biotic factors were analyzed with Canoco for Windows 4.5 software. There were 6 species of Hoya namely Hoya multiflora, Hoya imperialis, Hoya vitellinoides, Hoya hasseltii, Hoya campanulata and Hoya lacunosa found in this study. The highest biodiversity index was obtained in an altitude of 700-800 m.a.s.l (H’=1.41) and in hillsides area (H’=1.3). Hoya ‘s most frequent phorophyte was Schima wallichii. Factors affecting the presence of Hoya in the phorophytes were the position of attachment to the main stem and the rough surface characteristics of the tree. Hoya ‘s preference place was on the bark fracture. Abiotic factors that most influencing the existence of Hoya were air humidity and canopy cover. The data of this research can be used as basic information for carried out the conservation efforts and cultivation strategy in the future.
Evaluation of Soybean Resistance to Pod-Sucking Bug, Riptortus linearis F. and Performance of its Agronomic Characters Ayda Krisnawati; Mochammad Muchlish Adie
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12806

Abstract

Pod sucking bug, Riptortus linearis, is the most damaging soybean pest in Indonesia. A total of 24 soybean genotypes was identified for their resistance to pod sucking bug. The objective of the study was to classifying the resistance of some soybean genotypes against pod sucking pests. The research was conducted during the second dry season (July - October) 2016 in Ngale Research Station, East Java, Indonesia. The experimental design was arranged in randomized block design with five times repetition and two types of environment (full crop protection and controlled until 50 days of planting/dap). The resistance to pod sucking bug was evaluated based on seed damage, pod damage, and seed weight. The grouping of resistance was by using Chiang Talekar’s method (1980). A very high natural population of pod sucking bug was recorded in the research area as seen from the average number of damaged pod in full protection environment (L1) and insecticide control until 50 dap (L2), i.e. 41.45% and 60.16%, respectively. Genotype of G511H/Anj//Anj-2-8 was consistently resistant to pod sucking bug in L1 as well as L2. This genotype also had early days to maturity (78 days) and large seed size (15.57 g/100 seed), thus potentially to be developed in tropical area of Indonesia. The availability of genotype with such characteristics is in accordance with farmers’ preference and also important to minimize yield losses.
Perbaikan Fraksi Lipid Serum Tikus Putih Hiperkolesterolemi Setelah Pemberian Jus dari Berbagai Olahan Tomat (Hypercholesterolemia Albino Rat Lipid Fraction Improvement After Giving Tomato Juice) Retno Sri Iswari
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.45

Abstract

This research would like to show the effect of tomato juice in a variety processing method towards hypercholesterolemia albino rat blood plasma lipid fraction. The special purpose if to know and analyze tomato juice advantages towards total cholesterol content, LDL–cholesterol, HDL–cholesterol and triglyceride. The research design is a Post Test Randomized Control Design by means of Complete Randomized Design (RAL). There are 4 groups of treatment, each groups has 6 repeaters. The data is tested by One Way Varian Analyze. If there is a significance difference, there will be Duncan’s New Multiple Range Test followed. The research shows that there is a significance difference of total cholesterol content, LDL–cholesterol, HDL–cholesterol and triglyceride. The highest total cholesterol content, LDL–cholesterol and blood plasma triglyceride content are on the hypercholesterolemia control group. The lowest total cholesterol content, LDL–cholesterol and blood plasma triglyceride content are on the group that is given by a fried tomato juice. The lowest HDL content is on the hypercholesterolemia control group and the highest one are on the group that is given by a fried tomato juice. The research conclusion is that tomato juice supplementation able to fix hypercholesterolemia albino rat blood plasma lipid fraction, especially to decrease total cholesterol content, LDL–cholesterol and blood plasma triglyceride, and increase hypercholesterolemia albino rat blood plasma HDL-cholesterol content. Fried tomato juice give the best effect on decreasing total cholesterol content, LDL–cholesterol and triglyceride and increasing blood plasma HDL-cholesterol content. Keywords: hypercholesterolemia albino rat, total cholesterol content, LDL–cholesterol, HDL–cholesterol, triglyceride
Identification of Soybean Genotypes for Pod Shattering Resistance Associated with Agronomical and Morphological Characters Ayda Krisnawati; Mochammad Muchlish Adie
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8722

Abstract

A yield loss caused by pod shattering is one of the obstacles to the improvement of soybean productivity in tropical areas. The aim of this study was to identify the resistance of soybean genotypes to pod shattering as affected by agronomical and morphological characters. The field study was conducted in Malang, Indonesia, using 150 soybean genotypes. Data were collected on agronomical traits, the percentage of pod shattering, and pod morphological traits. Identification for shattering resistance was done as per oven dry method. Percentage of pod shattering was ranged from 0 % up to 100 % shattering with a mean of 58.11 %. Pod shattering was found to be negatively correlated with a number of pod per plant, the thickness of the pod, and Y/Z (seed weight and pod weight ratio). The Identification obtained 66 very highly susceptible genotypes, 19 susceptible genotypes, 19 moderate genotypes, 38 resistant genotypes, and 8 very resistant genotypes. Two of eight very resistant genotypes (G511H/Anj//Anj///Anj////Anj-6-11 and G511H/Anj//Anj///Anj////Anj-5-4) have high yield, medium maturity day and large seed size. Those lines could be used as gene donor for soybean varietal improvement for shattering resistance, and recommended to propose as new improved soybean varieties resistant to pod shattering in Indonesia. 
Characteristics of Cells from Five Exotic Bamboos after Drywood Termites Incisitermes minor (Hagen) Attack Niken Subekti; Tsuyoshi Yoshimura
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3952

Abstract

This study examined the relationship between and the characteristics of five bamboos exotic in the cell walls. For this purpose, the bamboosexotic from Indonesia  were separated into cells after drywood termites Incisitermes minor (Hagen) attack. SEM observations revealed that bamboos cells. Moreover, SEM analysis showed that the cellulose isolated from parenchyma cells. These results suggest that all the cellulose microfibrils in five bamboos different characteristics of cell function after drywood termite attack.Penelitian ini menguji hubungan antara karakteristik dan lima bambu eksotis di dinding sel. Untuk tujuan ini, bamboosexotic dari Indonesia dipisahkan ke dalam sel setelah rayap Drywood Incisitermes minor (Hagen) serangan. Pengamatan SEM menunjukkan bahwa sel-sel bambu. Selain itu, analisis SEM menunjukkan bahwa selulosa yang diisolasi dari sel parenkim. Hasil ini menunjukkan bahwa semua brils fi selulosa mikro di lima bambu karakteristik yang berbeda dari fungsi sel setelah serangan Rayap Kayu Kering.

Page 61 of 107 | Total Record : 1068

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