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Molekul: Jurnal Ilmiah Kimia

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Published by Universitas Jenderal Soedirman

ISSN : 19079761 EISSN : 25030310 DOI : -

Core Subject : Science, Engineering,

Chemical Engineering, Chemistry & Bioengineering Chemistry

MOLEKUL is a peer-reviewed journal of chemistry published by the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Indonesia. Publishing frequency 2 issues per year, on May and November. This Journal encompasses all branches of chemistry and its sub-disciplines including Pharmaceutical, Biological activities of Synthetic Drugs, Environmental Chemistry, Biochemistry, Polymer Chemistry, Petroleum Chemistry, and Agricultural Chemistry.

Arjuna Subject : -

Articles 331 Documents

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Enhanced Expression and Purification Strategy for Recombinant Bacterially-Expressed Human Hexokinase II Fazia Adyani Ahmad Fuad; Suriyea Tanbin
Molekul Vol 16, No 1 (2021)
Publisher : Universitas Jenderal Soedirman

Dengue virus hijacks the host cellular mechanism to propagate and survive during viral infection, in which the central carbon mechanism plays a crucial role to upregulate DENV infection through the increase of human hexokinase II (HKII) activity. Since the enzyme governs the glycolytic pathway, it has potentials as a target for anti-dengue (DENV) drug development. In this study, the production of human hexokinase II protein has been enhanced by using bacterial system for anti-dengue therapeutic purpose. The HKII gene was cloned into pET28b vector and transformed into the E. coli strain BL21 (DE3) for HKII expression. In order to obtain soluble recombinant HKII in an active form, we optimized protein expression under specific conditions at 18°C for 19 hours using Terrific Broth media, in the presence of 0.5 mM isopropyl-2-D-thiogalactopyranoside (IPTG). The pET28b-HKII construct expressed in BL21(DE3) system exhibited adequate protein expression, thus, this construct was subsequently proceeded to purification process. The expressed protein was purified to homogeneity by a combination of Immobilized Metal Ion Affinity Chromatography (IMAC)and size exclusion chromatography (SEC), resulting in pure, active bacterially-expressed HKII with a specific activity of 56. 67U.mg-1. The amount of HKII obtained from 2 L culture is 80 mg, with a yield percentage of 10.5%. Hence in this study, human HKII has successfully been cloned and expressed as a soluble protein that can be utilized for further therapeutic studies.

Light-induced synthesis of micron-sized metallic silver in aqueous extract of Rivina humilis L. fruits and its interaction with Corptotermes curvignathus Salprima Yudha S; Aswin Falahudin; Morina Adfa; Irfan Gustian; Herlina Herlina
Molekul Vol 16, No 2 (2021)
Publisher : Universitas Jenderal Soedirman

The aqueous extract of Rivina humilis was suitable to reduce silver ions (Ag+) to form micron-sized metallic silver at room temperature and without any addition of external reducing agent or stabilizer compounds. The reduction process was assisted by light, indicated by the colour change of the reaction mixture and supported by the appearance of peak at 455 nm in spectrophotometric analysis when the reaction was carried out under room light. Based on transmission electron microscopy (TEM) analysis, the as-prepared metallic silver was in spherical form. The analysis results using particles size analyzer (PSA) show their particles distribution from micro to nano size (average size was 199 nm). The interaction behaviour of micron-sized metallic silver/extract solution with Corptotermes curvignathus termites shows that the silver gives small additional effects along with the activity of the extract.

Cr/Al Pillared Bentonite and Its Application on Congo Red and Direct Blue Removal Muhammad Said; Afifah Rahma Dian; Risfidian Mohadi; Aldes Lesbani
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

Bentonite was modified through pillarization with metal oxide Cr/Al in 2:1 and 1:2 ratio to obtain larger basal spacing of bentonite-layered structure. Pillarization was conducted via intercalation method at room temperature followed by calcination. The result was characterized by using XRD and FT-IR Spectrophotometer. XRD pattern shown pillarization by Cr/Al 2:1 ratio gave peak at 2θ 5.3° which is correspond to d = 16.4 Å. At 1:2 Cr/Al ratio, peak appears at 2θ 6.4° that is related to d = 13.7 Å. FTIR Spectroscopy measurement gave inconclusive result. The Cr/Al pillared bentonite at 2:1 ratio was applied as adsorbent on Congo red and Direct blue dyes. The result indicates the adsorption follows Freundlich isotherm. Adsorption rate for both dyes follows pseudo-second order with rate constant of pillared bentonite higher than natural one. Adsorption isotherm concludes that Freundlich isotherm is favorable compare to Langmuir. Thermodynamic evaluation suggests that isotherm occurs spontaneously in an exothermic process.

Characterization of Antioxidant Compound from Syzygium polyanthum Leaves Fraction Using UHPLC-HRMS Muhammad Ana Syabana; Nancy Dewi Yuliana; Irmanida Batubara; Dedi Fardiaz
Molekul Vol 16, No 1 (2021)
Publisher : Universitas Jenderal Soedirman

The leaves of Syzygium polyanthum is widely used in Indonesia as a spice. It has been reported to have significant antioxidant activity. However, the reports on compounds responsible for the activity are very scarce. The purpose of this research was to characterize the chemical profile of S. polyanthumfractions which showed the highest antioxidant activity. The dried leaves of S. polyanthum were fractionated using the different combination of non-polar to polar solvent, those are n-hexane, acetone, water. Fourteen fractions were collected and analyzed for their DPPH antioxidant activity. The chemical profile of selected fractions was characterized using UHPLC-HRMS to identify compounds that might associate with the antioxidant activity of S. polyanthum. The results showed that at the dose of 500 μg/ml, four fractions showed the highest antioxidant activity with the following order: F9 (acetone:water=80:20), F10 (acetone:water=60:40), F1 (hexane 100%) andF3 (hexane:aceton=80:20). UHPLC-HRMSanalysis successfully identified several compounds in F9 and F10. Gallic acid, pyrogallol, and myricetin were identified in F9. Aconitic acid was identified in F10. All of the identified compounds were previously reported to have antioxidant activity

Ab-Initio Computational Study : The Activation Energy Changes and Steric Effects In Peptide Synthesis Of Ac-AA-NH2 and Ac-AP-NH2 Pratiwi, Indah; Cahyono, Bambang; Siahaan, Parsaoran
Molekul Vol 16, No 2 (2021)
Publisher : Universitas Jenderal Soedirman

Ab-Initio computational method can be used for simulating reaction mechanisms, such as concerted reaction mechanism on peptide synthesis. The concerted reaction is one of many possible pathways on how peptide can be synthesized. The purpose of this study are probing the concerted reaction mechanism and comparing the steric effect to the reaction, given by different side-chain of alanine (A) and proline (P). Two dipeptides formed from alanine and proline were computed at HF-SCF/6-31G** theory level: Ac-AA-NH2Â and Ac-AP-NH2.Â The res.lts show the activation energy ofÂ Ac-AA-NH2Â Â and Ac-AP-NH2Â forming via concerted pathway are 167.541 kJ/molÂ and 161.044 kJ/mol, respectively.Â The steric difference in side-chain affects the dihedral angle of the structure, and also gives difference to the entropy value of reaction.

DFT Study of Leuco-Indigo and Indigo as Active Material in Dye-Sensitized Solar Cell Maahury, Mirella Fonda; Male, Yusthinus Thobias; Martoprawiro, Muhamad Abdulkadir
Molekul Vol 15, No 2 (2020)
Publisher : Universitas Jenderal Soedirman

Computational study of natural indigo has been carried out to get the optimized structure and electronic properties of two indigo at different pH.Â It has been found that indigo has the potential to be applied asÂ active material in the dye-sensitized solar cell. Computational calculations are performed using Density Functional Theory (DFT) with B3LYP functional and 6-31G(d,p) for ground state geometry optimization and Time Dependent-Density Functional Theory (TD-DFT) for excited states single point calculation. All calculations are carried out in the gas phase. Geometry optimization results show that Indigo has a planar structure, whereas leuco-indigo structure is not planar. Leuco-indigo absorption wavelength is shorter than indigo. The density of HOMO and LUMO is spreading throughout the molecule. The density of electrons at LUMO extends to the anchoring group. Based on excitation energy, absorption wavelength, excitation composition, and HOMO-LUMO density, it can be concluded that indigoÂ has the potential to be applied inÂ dye-sensitized solar cell (DSSC)

Backmatter (Instruction for author and back cover) Fatoni, Amin
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

Backmatter (Instruction for author and back cover) of the print version

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents) Fatoni, Amin
Molekul Vol 16, No 1 (2021)
Publisher : Universitas Jenderal Soedirman

Frontmatter (Front Cover, Editorial Team, Preface and Table of Contents)

Phytochemical Analysis and Antioxidant Activity of Brotowali (Tinospora crispa L. Mier) Stem Warsinah, Warsinah; Baroroh, Hanif Nasiatul; Harwoko, Harwoko
Molekul Vol 15, No 2 (2020)
Publisher : Universitas Jenderal Soedirman

Free radical in the body could cause degenerative diseases such as diabetes mellitus and hyperlipidemia, which could be prevented by the supplementation of antioxidant whether it is synthetic or natural. Flavanoids is a phenolic compound, was extracted from natural sources is known for its antioxidative potential. This research aimed to investigate the active compounds in brotowali (Tinospora crispa) stem to find new antioxidative potential using its ability to bindÂ 2, 2â€™-diphenyl-1-picrylhydrazylÂ (DPPH). Brotowali stem was extracted using ethanol and fractioned usingÂ n-hexane, ethyl acetate, and water. Water fraction was hydrolyzed by chloric acid. The antioxidant activity of ethanol extract, water fraction, and hydrolized water fraction (subfraction I and subfraction II) were determined using spectrophotometry (DPPH methods). The compound of flavonoid was identified using KLT with AlCl3Â reagent spray. TheÂ results showed that ethanol extract andÂ water fraction of brotowali stemÂ contained flavonoid glycoside of flavonol group. Antioxidant activity, quantified using IC50, of ethanol extract, water fraction, subfraction I and subfractiont II were 49.92Î¼g/mL, 38.25Â Î¼g/mL, 36.12Â Î¼g/mL, and 16.18Â Î¼g/mL, respectively. In addition, this research was found that hydrolysis of water fraction using chloric acid like in subfraction I and subfraction II was able to improve its antioxidant activity.

Cation Sensing Capabilities of A Nitrophenyl Cinnamaldehyde Derivative Suryanti, Venty; Wibowo, Fajar Rakhman; Marzuki, Ahmad; Sari, Meiyanti Ratna Kumala
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

The cationic chemosensor based on organic compound bearing an aminophenol moiety as a receptor for metal analyte and a cinnamaldehyde moiety as chromophoric fragment has been developed. In this work, we report the colorimetric sensing of nitrophenyl cinnamaldehyde derivative, namely methyl-3-(2-hidroxy-5-nitrophenyl amino)-3-phenylpropanoate, towards a variety of metal cations, such as Cu2+, Fe3+, Ni2+ and Zn2+. The cation sensing abilities of the sensor were observed for Cu2+and Fe3+ with a color change from colorless to pink and faint yellow, respectively, The characteristic UV-Vis spectra changes were observed upon addition of Cu2+and Fe3+ cations. The hypsochromic absorption spectra shifts were obtained, indicating the cations and sensor complexations had formed. A metal-to-ligand-charge-transfer (MLCT) had occurred and the charge density of the sensor changed resulting in appearance of new absorption peaks in the UV-Vis spectra and color changes of the sensor solution upon addition of the Cu2+and Fe3+. Â

Page 26 of 34 | Total Record : 331

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Amin Fatoni

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Home Page

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Contact Name Amin Fatoni

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Editorial Address Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

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Contact Name
Amin Fatoni

Contact Email
aminfatoni@unsoed.ac.id

Phone
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Journal Mail Official
j.molekul@gmail.com

Editorial Address
Jl. Dr. Soeparno No.61 Karangwangkal, Purwokerto, Jawa Tengah 53

Location
Kab. banyumas,

Jawa tengah

INDONESIA