cover
Contact Name
Hayati Minarsih
Contact Email
menaraperkebunanppbbi@gmail.org
Phone
-
Journal Mail Official
menaraperkebunan@iribb.org
Editorial Address
Jalan Taman Kencana No.1 Bogor 16128, Jawa Barat
Location
Kab. bogor,
Jawa barat
INDONESIA
Menara Perkebunan
ISSN : 01259318     EISSN : 18583768     DOI : -
Core Subject : Agriculture,
Menara Perkebunan as a communication medium for research in estate crops published articles covering original research result on the pre- and post-harvest biotechnology of estate crops. The contents of the articles should be directed for solving the problems of production and/or processing of estate crops of smallholder, private plantations and state-owned estates, based on the three dedications of plantation. Analyses of innovative research methods and techniques in biotechnology, which are important for advancing agricultural research. Critical scientific reviews of research result in agricultural and estate biotechnology.
Arjuna Subject : -
Articles 8 Documents
Search results for , issue "Vol 88, No 1 (2020): April, 2020" : 8 Documents clear
Fortifikasi senyawa selenium pada jamur tiram coklat (Pleurotus pulmonarius dan Pleurotus sajor-caju) Firda DIMAWARNITA; Yora FARAMITA; . TRI-PANJI
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.798 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.353

Abstract

Selenium (Se) is one of the most important micronutrients needed for human health. However, the content of Se compound in animals and plants isrelativelysmallin order to meet recommended intakes of Se. Therefore, Se fortification in food source, such as mushroom, is needed. In this study, fortification was carried out by adding sodium selenite to the growth media (the mixture of sawdust and OPEFB) of brown oyster mushrooms included Pleurotus pulmonarius and Pleurotus sajor-caju, in various concentrations of 100, 200, 300, and 400 ppm. The results showed that the highest absorption of Se in P. pulmonarius and P. sojur caju (3.51 and 2.31 %, respectively) obtained from 200 ppm sodium selenite addition. High concentrations of sodium selenite in baglog media tend to inhibit mycellium growth and the production of mushroom fruiting body. The additions of 200 ppm sodium selenite in baglog media of P. pulmonarius and P. sajor-caju were the best treatment in term of the fastest mycelium coverage in 40 and 37 days with the highest biological efficiency ratio (BER) value of 18.80 and 17.89 %, respectively.Layu Senyawa selenium (Se) merupakan salah satu mikronutrien terpenting yang harus dipenuhi kebutuhannya dalam tubuh. Akan tetapi, kandungan senyawa Se pada hewan dan tumbuhan sangat kecil untuk memenuhi kebutuhan asupan Se yang dianjurkan. Oleh karena itu, fortifikasi Se pada sumber pangan seperti pada jamur diperlukan. Dalam penelitian ini, fortifikasi dilakukan dengan menambahkan sodium selenit ke dalam media pertumbuhan (campuran serbuk gergaji dan TKKS) jamur tiram coklat, yaitu Pleurotus pulmonarius dan Pleurotus sajor-caju dengan berbagai konsentrasi,diantaranya: 100, 200, 300, dan 400 ppm. Hasil penelitian menunjukkan penyerapan Se tertinggi pada P. pulmonarius dan P. sojurcaju yaitu masing-masingsebesar 3,51 dan 2,31% diperoleh dari penambahan sodium selenit 200 ppm. Konsentrasi sodium selenit yang tinggi pada media baglog cenderung menghambat pertumbuhan miselium dan produksi tubuh buah jamur. Penambahan sodium selenit sebanyak 200 ppm pada media baglog P. pulmonarius dan P. sajor-caju merupakan perlakuan terbaik ditinjau dari waktu tercepat pertumbuhan miselium jamur menutupi media dalam baglog, yaitu masing-masing 40 dan 37 hari dengan nilai biological efficiency ratio (BER) tertinggi, yaitu 18,80 dan 17,89 % secara berurutan.
SPAD-502 and atLEAF CHL PLUS values provide good estimation of the chlorophyll content for Hevea brasiliensis Müll. Arg. Leaves Andi Nur CAHYO; Rudi Hari MURTI; Eka Tarwaca Susila PUTRA; Tri Rini NURINGTYAS; Denis FABRE; Pascal MONTORO
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.369

Abstract

Measurement of chlorophyll content using destructive methods is not efficient due to a large number of samples, cost, and time needed. Estimationof chlorophyll content by nondestructive methods using handheld chlorophyll meter may be considered to improve efficiency. This research aimed to determine the formula to convert SPAD-502 and atLEAF CHL PLUS values (relative indicator of chlorophyll content) to estimated (absolute) rubber leaves chlorophyll content. Twenty leaves of rubber plant were measured using SPAD-502 and atLEAF CHL PLUS at the same time to determine SPAD-502 and atLEAF CHL PLUS values. The measured leaves were then collected to determine the chlorophyll content using a standard laboratory procedure. Regression and correlation analyses (among 3 methods) were conducted using SAS v.9 software. The results showed that between SPAD-502 and atLEAF CHL PLUS values were closely correlated, hence both of the devices can substitute each other to estimate rubber leaf chlorophyll content. In addition, the relationship between atLEAF CHL PLUS and SPAD-502 values with actual chlorophyll content of rubber clone SP 217, PB 260, GT1, and all clones (general) were significant with high coefficient of determination (R2) as well as low Root Mean Square Error (RMSE) and Coefficient of Variation (CV). Therefore, by using formula determined in this study, both atLEAF CHL PLUS and SPAD-502 can be suggested for accurate, fast, and non-destructive estimation of chlorophyll content of rubber plant leaf.
Sintesis dan uji in vitro penghambatan nanokitosan-Cu terhadap pertumbuhan Fusarium oxysporum dan Colletotrichum capsici Sri WAHYUNI; Muhammad Alfian PRASETYO; Deden Dewantara ERIS; . PRIYONO; . SISWANTO
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.338 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.367

Abstract

Wilt and anthracnose are diseases on chili that cause substantial losses and even crop failure. Control of the diseases is generally carried out using chemical pesticides which are environmentally harmful. Therefore, the development of nanoparticles, such as nanochitosan-Cu, can be an environmentally friendly solution in controlling chili disease. The current technology in developing nanochitosan-Cu is green synthesis, which uses an effective reducing agent but non-toxic for plants and the environment. However, the process requires sonication, which is difficult to be adapted for scale-up production. This research aimed to synthesize and determine the formulation of nanochitosan –Cuusing magnetic stirrer method without sonication, and also to evaluate the antifungal ability of nanochitosan-Cu againstFusarium oxysporum and Colletotrichum capcisi causing wilt and anthracnose disease, respectively. Synthesis of nanochitosan-Cu was carried out at 50°C and 400 rpm of speed. Characterizations of nanochitosan-Cu were analyzed using SEM-EDX and PSA. In vitro antifungal activity test was carried out by food poisoning method.The results showed that the synthesis of nanochitosan-Cu using ionic gelation method can be carried out without sonication process, and produce round shape nanoparticles with 183.7 nm of diameter. The nanochitosan-Cu was effective against F. oxysporum and C. capsici, at a concentration of 100 ppm. It inhibited the growth of F. oxysporum and C. capsici by 100% and 92.38%, respectively.Layu dan antraknosa merupakan penyakit pada tanaman cabai yang menimbulkan kerugian besar bahkan gagal panen. Pengendalian penyakit tersebut umumnya dilakukan menggunakan pestisida kimia yang tidak ramah lingkungan. Untuk itu perlu dikembangkan biofungisida yang ramah lingkungan, salah satunya adalah nanopartikel kitosan-Cu. Teknologi pengembangan nanopartikel yang saat ini sedang berkembang adalah green synthesis, yaitu menggunakan reduktor yang efektif namun tidak toksik terhadap tanaman dan lingkungan. Proses sintesis ini membutuhkan sonikasi yang cukup sulit untuk diadaptasi pada skala produksi masal. Penelitian ini bertujuan untuk melakukan sintesis nanopartikel kitosan-Cu dengan metode magnetic stirrer tanpa sonikasi, serta mengevaluasi kemampuan antifungi terhadap Fusarium oxysporum dan Colletotrichum capcisi penyebab layu dan antraknosa. Sintesis nanokitosan-Cu dilakukan pada suhu 50°C dengan kecepatan 400 rpm. Karakterisasi nanokitosan-Cu dilakukan menggunakan SEM-EDX dan PSA. Uji aktivitas antifungi secara in vitro dilakukan menggunakan metode peracunan makanan. Hasil penelitian menunjukkan bahwa sintesis nanokitosan-Cu dengan metode gelasi ionik dapat dilakukan tanpa proses sonikasi dan menghasilkan nanopartikel berdiameter 183,7 nm dan berbentuk bulat. Nanokitosan-Cu yang dihasilkan efektif sebagai antifungi terhadap F. oxysporum dan C. capsici. Pada konsentrasi 100 ppm, nanokitosan-Cu mampu menghambat pertumbuhan F. oxysporum dan C. capsici masing-masing sebesar 100% dan 92,38%.
Bioconversion performance and development of black soldier fly (Hermetia illucens L.) on treated cocoa pod husk Ciptadi Achmad YUSUP; Haryo Tejo PRAKOSO; . SISWANTO; Deden Dewantara ERIS
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.524 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.365

Abstract

Indonesia is the third largest cocoa producer in the world, thus the number of cocoa pod husk (CPH) resulted from this activity is abundant. To handle this waste, farmer usually uses it directly as a feed source to small ruminants but this practice is less effective due to its low protein content and it also contains a substantial amount of lignin. Black Soldier Fly (BSF) (Hermetia illucens L.) (Diptera: Stratiomyidae) larvae are known as bioconversion agents that can be fed upon various organic substrates and they are also high protein source. The aim of this research was to evaluate the possibility of BSF grown on CPH based on their relative growth rate (RGR), efficiency of conversion of ingested food (ECI), waste reduction index (WRI), and development time. Body size of the imago from each treatment was also measured. Larvae were fed with fresh CPH (F), fresh blended CPH (B), composted CPH (C), mix of fresh CPH with food waste (F+FW) and mix of composted CPH with food waste (C+FW). Food waste served as a control. The results of this study show that the most ideal treatment that possible to be applied in cocoa plantation was C+FW treatmentwhich gave average prepupal fresh weight of 11.20 g/100 larvae with 18 days of development time. This treatment had the highest value of WRI and RGR among all treatments. Composted CPH that mixed with food waste treatment also had a shorter development time of BSF larvae.
Potential use of Claroideoglomus etunicatum to enrich signal grass (Brachiaria decumbens Stapf.) for silvopasture preparation Risa ROSITA; Rahayu WIDIASTUTI; Irdika MANSUR; Sarah Asih FAULINA
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.391 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.364

Abstract

Silvopasture system improvement in managing post-mining land resources has been done by searching for a quality grass. One of the selected grass species is signal grass (Brachiaria decumbens Stapf.). This research aimed to prepare signal grass through the inoculation of AMF Claroideoglomus etunicatum, as an effort to enrich its growth before being applied to post-mining soil. Research stages included the AMF inoculation on signal grass through spore culture and then transferred the colonized grass to the pot using sterile zeolite as a growth medium. The treatment on the first stage was without and with AMF inoculation (dose of 20 spores) on signal grass which was repeated for 12 times. Incubation in a spore culture was 4 weeks while incubation in a pot containing sterile zeolite medium was 8 weeks. Research data were analyzed using the Shapiro-Wilk’s normality test, Independent Sample T-test, and Pearson’s correlation test. Observation results showed that the inoculation of C. etunicatum on signal grass was significantly impact on the increase of plant height, stem diameter, number of leaves, number of tillers, shoot and root fresh weight, and shoot dry weight (p <0.05). Microscopic observation showed that there was AMF colonization on treated signal grass roots in the amount of 55 ± 0.06 % with number of spores was 252 ± 9.82 per 10 g zeolites, while AMF infection was not found in uninoculated signal grass. It is expected that by providing signal grass inoculated with AMF C. etunicatum would support its growth in post-mining land for Silvopasture system.
Isolation and characterization of Dehydrin promoter region from sugarcane (Saccharum officinarum L.) Hayati MINARSIH; Sonny SUHANDONO; Anissa K FUADI; Tati KRISTIANTI; Riza A PUTRANTO; Deden SUKMAJDAYA; . SUSTIPRIJATNO
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (867.853 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.363

Abstract

The development of molecular biology techniques nowadays has enabled to engineer drought tolerant sugarcane by genetic engineering to accelerate the breeding program. Dehydrin (DHN) is known to have an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). While plant tissues are subjected to drought stress (dehydration), DHN protein is accumulated to high content throughout all vegetative or generative tissues. The research aimed to isolate and characterize the DHN promoter from sugarcane that can be used as transformation material in generating drought tolerant sugarcane. Specific primers for DHN promoter amplification were designed and DHN promoter region was successfully isolated by PCR cloning method. Two putative promoter sequences were identified namely Pr-1DHNSo and Pr-2DHNSo. In silicoanalyses were carried out and cis-regulatory elements motifs that play a role in adaptation on abiotic stress as well as biotic stress including ABRE, MBS, CGTCA-motif, TGACG-motif, GARE-motif, P-box TCA-element and Box-W1 were identified. The promoter Pr-1DHNSo was then cloned into pBI121 expression vector by Overlap Extention PCR (OE-PCR) for further characterization. Functional test of the promoter construct pBI- Pr-1DHNSo was conducted through Agrobacterium transformation into sugarcane calli. GUS assay and PCR analysis showed that the DHN promoter was transformed and expressed in the sugarcane calli.
Application of organic fungicide in controlling basal stem rot disease for mature oil palm Happy WIDIASTUTI; Hayati MINARSIH; Djoko SANTOSO; Deden Dewantara ERIS; Galuh Wening PERMATASARI
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.537 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.368

Abstract

Ganoderma is a major pathogen in oil palm crops. Some efforts related to control the growth of Ganoderma have been conducted but still have not found an effective method. This study aims to develop an organic fungicide that has been tested in vitro, which effective in controlling the growth of Ganoderma. The optimization carried out includes the determination of the dose and time interval for application in 13-year-old mature oil palm. This organic fungicide application was the continuation of application during the previous year especially for the two best treatment which is application organic fungicide every week (1w) and every two weeks (2w). In this study, the treatments tested were three levels dose of organic fungicide (0, 1x and 2x) and two types of frequency application, i.e. every week (1w) and every other week (2w). The results showed that the best application of organic fungicides was every week application with twice doses (1w.2x), based on the parameters of the inhibition of Ganoderma’s fruiting body formation, primary and secondary root formation, the opening of spear leaves, and harvesting parameters. The application of organic fungicide able to recover the oil palm infected Ganoderma sp., with increasing the fresh fruit bunch and its weight around 70% and 78%, respectively.
Changes in chemical constituents and overall acceptability of papaya jam fortified with soya protein during storage Dimas Bayu PINANDOYO; Arisadi MASNAR
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.361

Abstract

Processed fruit products commonly contain very low protein due to the heat treatment during processing period. This article presents the researchresults concerning the increase of nutrition value of papaya jam fortified with soya protein concentrate. Papaya jam prepared with ratio 40:1, 40:2, 40:3, 40:4, and 40:5 (v/v) of papaya pulp and soya protein concentrate. The treatment of papaya jam and soya protein with the highest acceptability was then analyzed for the changes in chemical constituents and overall acceptability at monthly interval during three months of storage. The parameters that being analyzed were moisture content, total soluble solids (TSS), pH, total and reducing sugars, non-enzymatic browning, acidity, ascorbic acid, total carotenoid, total phenol, crude protein, and total plate count.  The results showed that fortified papaya jams prepared with papaya pulp and soy protein concentrate ratio at 40:1 had the highest acceptability. During storage time it revealed that moisture content, TSS, pH, total and reducing sugars, and non-enzymatic browning increased, while acidity, ascorbic acid, total carotenoids, phenols, total antioxidants and crude protein contents of jam decreased. Fortification of papaya jam with soya protein concentrate (40:1 v/v) increased crude protein content on average from 3.15% to 4.16%. Total plate count (TPC) indicated that no microbial contamination during 3 months storage period. It can be concluded that papaya jam fortified with soya protein concentrate remained acceptable during storage although the acceptability decreased over time.

Page 1 of 1 | Total Record : 8


Filter by Year

2020 2020


Filter By Issues
All Issue Vol. 93 No. 1 (2025): 93(1), 2025 Vol. 92 No. 2 (2024): 92(2), 2024 Vol. 92 No. 1 (2024): 92(1), 2024 Vol. 91 No. 2 (2023): 91 (2), 2023 Vol. 91 No. 1 (2023): 91 (1), 2023 Vol. 90 No. 2 (2022): 90 (2), 2022 Vol. 90 No. 1 (2022): 90 (1), 2022 Vol 90, No 2 (2022): Oktober, 2022 Vol. 90 No. 2 (2022): Oktober, 2022 Vol 90, No 1 (2022): April, 2022 Vol. 89 No. 2 (2021): 89 (2), 2021 Vol. 89 No. 1 (2021): 89 (1), 2021 Vol 89, No 2 (2021): Oktober, 2021 Vol 89, No 1 (2021): April, 2021 Vol. 88 No. 2 (2020): 88 (2), 2020 Vol. 88 No. 1 (2020): 88 (1), 2020 Vol 88, No 2 (2020): Oktober,2020 Vol 88, No 1 (2020): April, 2020 Vol. 87 No. 2 (2019): 87 (2), 2019 Vol. 87 No. 1 (2019): 87 (1), 2019 Vol 87, No 2 (2019): OKTOBER, 2019 Vol 87, No 1 (2019): April, 2019 Vol. 86 No. 2 (2018): 86 (2), 2018 Vol. 86 No. 1 (2018): 86 (1), 2018 Vol 86, No 2 (2018): Oktober 2018 Vol 86, No 1 (2018): April, 2018 Vol. 85 No. 2 (2017): 85 (2), 2017 Vol. 85 No. 1 (2017): 85 (1), 2017 Vol 85, No 2 (2017): Oktober 2017 Vol 85, No 1 (2017): April, 2017 Vol. 84 No. 2 (2016): 84 (2), 2016 Vol. 84 No. 1 (2016): 84 (1), 2016 Vol 84, No 2 (2016): Desember 2016 Vol 84, No 1: Oktober 2016 Vol. 83 No. 2: 83 (2), 2015 Vol. 83 No. 1: 83 (1), 2015 Vol 83, No 2: Desember 2015 Vol 83, No 1: Juni 2015 Vol. 82 No. 2: 82 (2), 2014 Vol. 82 No. 1: 82 (1), 2014 Vol 82, No 2: Desember 2014 Vol 82, No 1: Juni 2014 Vol. 81 No. 2: 81 (2), 2013 Vol. 81 No. 1: 81 (1), 2013 Vol 81, No 2: Desember 2013 Vol 81, No 1: Juni 2013 Vol. 80 No. 2: 80 (2), 2012 Vol. 80 No. 1: 80 (1), 2012 Vol 80, No 2: Desember 2012 Vol 80, No 1: Juni 2012 Vol. 79 No. 2: 79 (2), 2011 Vol. 79 No. 1: 79 (1), 2011 Vol 79, No 2: Desember 2011 Vol 79, No 1: Juni 2011 Vol. 78 No. 2: 78 (2), 2010 Vol. 78 No. 1: 78 (1), 2010 Vol 78, No 2: Desember 2010 Vol 78, No 1: Juni 2010 Vol. 77 No. 2: 77 (2), 2009 Vol. 77 No. 1: 77 (1), 2009 Vol 77, No 2: Desember 2009 Vol 77, No 1: Juni 2009 Vol. 76 No. 2: 76 (2), 2008 Vol. 76 No. 1: 76 (1), 2008 Vol 76, No 2: Desember 2008 Vol 76, No 1: Juni 2008 Vol. 75 No. 2: 75 (2), 2007 Vol. 75 No. 1: 75 (1), 2007 Vol 75, No 2: Desember 2007 Vol 75, No 1: Juni 2007 Vol. 74 No. 2: 74 (2), 2006 Vol. 74 No. 1: 74 (1), 2006 Vol 74, No 2: Desember 2006 Vol 74, No 1: Juni 2006 Vol. 73 No. 2: 73 (2), 2005 Vol. 73 No. 1: 73 (1), 2005 Vol 73, No 2: Desember 2005 Vol 73, No 1: Juni 2005 Vol. 72 No. 2: 72 (2), 2004 Vol. 72 No. 1: 72 (1), 2004 Vol 72, No 2: Desember 2004 Vol 72, No 1: Juni 2004 Vol. 71 No. 2: 71 (2), 2003 Vol. 71 No. 1: 71 (1), 2003 Vol 71, No 2: Desember 2003 Vol 71, No 1: Juni 2003 Vol. 70 No. 2: 70 (2), 2002 Vol. 70 No. 1: 70 (1), 2002 Vol 70, No 2: Desember 2002 Vol 70, No 1: Juni 2002 Vol. 69 No. 2: 69 (2), 2001 Vol. 69 No. 1: 69 (1), 2001 Vol 69, No 2: Desember 2001 Vol 69, No 1: Juni 2001 Vol. 68 No. 2: 68 (2), 2000 Vol. 68 No. 1: 68(1), 2000 Vol 68, No 2: Desember 2000 Vol 68, No 1: Juni 2000 More Issue