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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Search results for , issue "Vol 5, No 2 (2018)" : 12 Documents clear

KETERSERAPAN SENYAWA AKTIF TERPILIH EKSTRAK TEPUNG TEMPE KEDELAI HITAM dan UBI JALAR UNGU pada TIKUS MODEL DIABETES MELLITUS TIPE II Syahron Mahbub Balada
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Black soybeans and sweet potatoes contain antioxidants that can inhibit cell damage caused by free radicals. The aim of this study was to determine the absorption of selected active compounds (Isoflavones and Anthocyanins) in type II diabetes (DMT2) rat models. Wistar male rats were used as DMT2 models treated with high fat diet (HFD) and Streptozotosin (STZ) injections until the blood glucose levels reached 160 mg/dl. The experiment was carried out by giving extract of fermented black soybean, which is locally called Tempe, and sweet potato for 28 days on 9 Wistar rats. Rats models were divided into 3 treatment groups. Group I was given with the extract of fermented black soybean flour, group II was given with sweet potato extract, and group III was given with the extract of both fermented black soybean and sweet potato extract with 2:2 ratio. The sample of serum, fecal, and mouse urine were analyzed in the laboratory test by the method of High Pressure Liquid Chromatography (HPLC). The result showed that the absorption of Isoflavones and Anthocyanins extracted from fermented black soybeans and sweet potatoes in type II diabetes rat models were high (99,9%). Therefore, fermented black soybeans and sweet potatoes extract can be used as alternative antidiabetic remedy.

PERBEDAAN DOSIS ALUMINIUM SULFAT (TAWAS) TERHADAP KUALITAS SPERMATOZOA DAN KADAR ALUMINIUM DALAM TESTIS MENCIT (Mus Musculus) SWISS WEBSTER Nuraini Hidayah Khasanah; Gratiana Ekaningsih Wijayanti; Sorta Basar Ida Simanjuntak
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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This study designated to evaluate the quality of reproductive organs system in the form of testicular weight, testicular volume and weight of epididymis; quality of spermatozoa include concentration, viability, motility, and morphology of spermatozoa; and aluminum accumulation in mice testis after administration of different aluminum sulfate dose, i.e., 0, 122,5, 245 and 490 mg/kg bb for 45 days were orally administrated using feeding tube through oral route for 45 days. The next objective is to evaluate the correlation between the doses of aluminum sulfate with each variable, as well as to assess the most influential doses of aluminum sulfate in reducing testicular weight and volume, epididymal weight, concentration, viability, motility, and morphology of cauda epididymal spermatozoa and increasing aluminum levels in testes Mice. The results showed that aluminum sulfate did not significantly (p>0.05) decrease testicular weight, testicular volume, epididymal weight, concentration and viability of spermatozoa, but significantly (p<0.05) decreased spermatozoa motility at doses of 245 and 490 mg/kg bb and reduce the proportion of spermatozoa with normal morphology at 490 mg/kg BW. The decrease of motility and percentage of spermatozoa with normal morphology is inversely proportional to the dose of aluminum sulfate each with the value of r = -0.034 and r = -457. Administration of aluminum sulfate for 45 days did not increase aluminum levels in the testes of mice. The use of aluminum sulfate in food processing or drinking water is still safe for human reproductive health.

FITOREMEDIASI AIR LIMBAH PENCELUPAN BATIK PARAKANNYASAG, TASIKMALAYA MENGGUNAKAN KI APU (Pistia stratiotes L.) Tri Cahyanto; Tony Sudjarwo; Shinta Putri Larasati; Afriansyah Fadillah
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Batik home industry discharge untreated wastewater that can cause pollution to sewage water and its environment. Phytoremediation is the utilization of plants for environmental clean-up or reduces harmful contaminants including heavy metals such as chrome (Cr) of batik dyes. Water cabbage (Pistia stratiotes L.) locally named as Ki Apu is an aquatic plant known for its ability to reduce the Cr level in wastewater. This study aimed to determine the efficiency Ki Apu to reduce Cr level from batik dyeing wastewater from Parakannyasag, Tasikmalaya. This study was an experimental using Ki Apu from Indihiyang paddy field of Tasikmalaya. 12 individual of Ki Apu were grown on a 20 L water tank with different batik dyeing wastewater concentration of 0%, 25%, 50%, 75%, and 100%, each with five replicates. After 14 days the results showed the highest Cr reduction was measured at 77.5% occurred in 100% wastewater tank. Ki Apu leaf changed its leaf chlorophyll content with the highest estimate of 0.4 mg/L for chlorophyll-b and 1.3 mg/ L for the total chlorophyll; both occurred in 75% wastewater tank. The highest rate of phytoremediation indicated by BOD measure was at 33.4 mg/L/day occurred in 100% wastewater tank and by turbidity measure was at 23.3 mg/L/day occurred in 75% wastewater tank. Ki Apu is considered having a high phytoremediation effectivity to reduce Cr content in batik dyeing wastewater in Parakannyasag, Tasikmalaya.

KEANEKARAGAMAN UDANG AIR TAWAR (DECAPODA: CARIDEA) DI SUNGAI BATUSUYA, SULAWESI TENGAH, INDONESIA Diky Dwiyanto; Fahri Fahri; Annawaty Annawaty
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Sulawesi is one of the four Greater Sunda Islands characterized by rivers and lakes habitats of freshwater shrimps and prawns with a high degree of endemism. The freshwater shrimp and prawns diversity in the Batusuya river of Donggala is less studied compared to the ancient lakes in the Central Sulawesi area. This study aimed to determine the diversity of freshwater shrimp and prawns of the Batusuya river. The sample was collected in November 2016 using tray net. The study site divided into three sampling stations based on the type of habitat. Nine species of freshwater shrimps and prawns were found, i.e., Macrobrachium australe, M. esculentum, M. horstii, M. lar, M. placidulum, Caridina brevicarpalis, C. gracilipes, C. weberi, and Atyopsis spinipes. Six species were found in all localities including Macrobrachium australe, M. esculentum, M. horstii, M. lar, C. brevicarpalis, and C. gracilipes, while M. placidulum, C. weberi, and A. spinipes were not found in all stations. The highest diversity index (H') was found in the station III indicated freshwater shrimps and prawns favored the environmental conditions of this station. The highest measure of species richness (R) was found in the station I and presumably related to the life cycle of freshwater shrimps and prawns which is amphidromous. The occurrence of nine species of freshwater shrimps and prawns along the Batusuya river indicated the environmental condition of the stream was suitable for most freshwater shrimps and prawns, and the Macrobrachium australe was found as the most abundant species.

PENGARUH AIR REBUSAN DAUN MAMPELAS (Teteracera indica) TERHADAP AKTIVITAS MOTORIK MENCIT Zico Fakhrur Rozi
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Plants (Tetracera indica) commonly used by communities Musi North Rawas District for a variety of treatments. This study aims to see the effect of the use of leaf memelas to motor movement male mice. This research is true experimental with the design of Post-test only with control group design. 25 male mice divided into 5 groups. P0 was given 0 g leaf mampelas, P1 was given 2 g leaf mampelas, P2 was given 5 g leaf mampelas, P3 10 g leaves mampelas and P4 extra joss 10 mg. Treatment activities for 7 days. The calculation of motor motion is done by performing a series of tests with each of 3 repetitions, which consist of negative geotaxis test, swing test and swimming test. From the result of statistical analysis using Kruskal Wallis test, it is known that the boiled leaf water does not affect the negative geotaxis test (0,33>0,05), does not affect the hanging test (0,57>0,05) and swimming endurance Based on the above data it can be concluded that boiling water does not affect the influence of motor activity of mice.

FAKTOR RESIKO GANGGUAN FUNGSI GINJAL PADA PEKERJA BENGKEL LAS DI KOTA PURWOKERTO Anjar Astuti Ferdhiani; Hernayanti Hernayanti; Bambang Heru Budianto
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Welding was one of the industry in Purwokerto City. One of the exhaust particulate material from the welding process, this will be the potential occurrence of exposure to workers and caused renal dysfunction. Several risk factors have contributed in caused kidney difunction of workers. This study aims to determine the levels of cadmium metal (Cd), chromium (Cr), lead (Pb) with creatinine and Glomerulus Filtration Rate (GFR). The correlation of risk factors for renal dysfunction with creatinine and GFR. The risk factors that play a major role in caused kidney dysfunction. The method used is cross sectional. Sampling using purposive sampling with giving inform consent to the respondent. Provision of questionnaires and blood sampling as much as 3 ml according to ethical clearens. Measurement of metal content of Cd, Cr and Pb; serum creatinine and LFG levels. Number of respondents 30 people with 95% confidence level. The results showed that levels of Cr, Cd and Pb were 0.049 + 0.12 ppm; 1,029 + 2.38 ppm; 4,933+ 11,66 ppm. Cr levels are still within normal limits with levels but Cd and Pb exceeding the normal threshold. Creatinine levels of 1.867 + 0.446 mg / dl showed higher than normal. Minimum of GFR decrease in welding workers was 44 mL / min / 1.73 m2. Risk factors significantly correlated with creatinine and GFR was levels of Pb (p <0.05). Risk factors that play a major role in caused kidney dysfunction in welding workers in Purwokerto city is Pb content with 12.9% percentage effect on serum creatinine level and 9% effect on Glomerulus Filtration Rate.

BIRDS AT SEBYAR RIVER, ARANDAY, BINTUNI, WEST PAPUA Freddy Pattiselanno; Agustina Yohanna Setyarini Arobaya; Alfredo Ottow Wanma
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The River Sebyar is known as the home for particular aquatic birds and the wintering ground for migrant birds. The mass transportation along the river most likely impacted by the presence of wildlife species including birds. The research investigated the presence of water birds along the river. The study was done at six villages along the River Sebyar at the Aranday District of Bintuni Bay Regency of West Papua. The methods involved direct observation to the study sites by canoe-transect line along the river length with the assistance of binoculars, taxonomic description guide, and daily diary updated. Twenty-three birds were observed during the survey. Ten out of 23 bird species recorded in this survey have been observed that consisted of five species of Ardeidae, four of Anatidae, and one of Laridae. The escalation of human activities along the riverside especially during sago harvesting and a busy boat traffics within the Bintuni Bay tended to decrease spaces for particular bird species that usually occupied the areas for roosting and foraging assemblages.

BIODEGRADATION OF DIESEL OIL BY YEAST ISOLATED FROM MANGROVE’S RHIZOSPHERE Ryan Firman Syah; Agus Irianto; Nuniek Ina Ratnaningtyas
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Diesel oil-degrading yeast strains isolated from mangrove rhizosphere at Tritih Kulon, Cilacap had been screened with SMSS medium. Four culturable yeast were isolated. Qualitative test was conducted by culturing and incubating the yeasts for one month in a medium added with 1mL diesel oil. By measuring the reduction of diesel oil, two best yeasts were selected. The quantitative test, GC-MS analysis, was conducted to determine the detailed degradation process of diesel oil. Candida lusitaniae and Cryptococcus laurentii performed the degradative ability. Three highest percent area of hydrocarbon compounds were compared for assessment. The results showed that C. lusitanie had better degradative capability than C. laurentii, in which hexadecane and methyl hexadecanoate decreased by 90–95%, and 9-octadecenoic acid, methyl ester declined by 30–40%. The increasing pH medium during incubation suggested that fermentation process occurred.

APPLICATION OF PROTEASE ENZYMES IN PROTEIN UTILIZATION AT GROWTH OF WHITE SNAPPER FISH (Lates calcalifer) Fitria Aditama; Johannes Hutabarat; Subandiyono Subandiyono
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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White Kakap (Lates calcalifer) is a superior commodity and has a high nutritional value. Growth factors are hampered by the ineffectiveness of the digestive process. Protease is an enzyme capable of hydrolyzing peptide bonds in proteins. Feed is a factor that predominantly affects the growth of fish because feed serves as a supplier of energy to support growth. Protein digestion in aquatic animals requires proteases as a catalyst. Enzymes that play an important role in the process of protein digestion are proteases. The purpose of this study is to examine the effect of adding different protease enzymes to artificial feed on the efficiency of protein utilization in the growth of white snapper (Lates calcalifer). The results of this study are expected to provide information about the importance of protease enzyme application in artificial feed in increasing protein hydrolysis, so that protein can be easily utilized by the body of white snapper (Lates calcalifer). The cultivars tested were white snapper (Lates calcalifer). The cultivation comes from intensively cultivated Lampung with an average weight of 6.5 ± 0.44 g / tail with a length ranging from 4-5 cm, as many as 75 heads. The research method is experimental laboratories using Completely Randomized Design (RAL). The tested feed was in the form of artificial feed with protein content of 40% and different protease dosage. The tested treatment was A feed (0.0% Protease enzyme from artificial feed weight), B (0.05% Protease enzyme from artificial feed weight), C (0.1% Protease enzyme from artificial feed weight), D (0 , 15% Protease enzyme from artificial feed weight), E (0.2% Protease enzyme from artificial feed weight). The ingredients of white snapper fish feed consist of fish meal, corn flour, soybean meal, shrimp flour, fish oil, corn oil, CMC / wheat flour, minerals and vitamins. Research data obtained for 40 days using analysis of variance (anova). If there is a real effect on the treatment then Duncan multiple area test is done. The result of this research indicated that the giving of protease 0,2% in artificial feed with protein 40% able to produce feed consumed Kakap Putih 43,43 ± 0,13 g, FCR 0,35 ± 0,002, Feed Pureness equal to 67,9 ± 1.23%, Protein Digestibility 98.25 ± 0.03%, Protein Retention 68.8 ± 0.23%, PER 7.97 ± 0.04, Fat Retention 0.6 ± 0.005%, E / P 4, 6 cal / g, thus giving effect on Specific Growth (SGR) 6.032 ± 0.17% and Length 8.3 ± 0.2 cm.

KEANEKARAGAMAN ECHINODERMATA (ECHINOIDEA DAN HOLOTHUROIDEA) DI PULAU BAKALAN, BANGGAI KEPULAUAN, SULAWESI TENGAH, INDONESIA Moh Reza Sese; Annawaty Annawaty; Eddy Yusron
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The coastal zone of Indonesia, especially in the eastern archipelago, is composed of typical tropical small island ecosystems such as estuaries, coral reefs, seagrass meadows, algal beds, and sandy beaches which are habitats of many groups of marine organisms including Echinoderms. The biodiversity of many small islands in this area was understudied, including Bakalan Island of Banggai Islands in Central Sulawesi. The purpose of this study was to determine the diversity of Echinoderms (Echinoidea and Holothuroidea) in Bakalan Island. This study applied the purposive sampling technique with the 2 x 2 m quadrats were placed along a 100-meter transect with ten-meter intervals. There are nine species of echinoderms found in this study including Salmacis sphaeroides, Mespilia globulus, Tripneustes gratilla, Echinometra mathaei, Diadema setosum, D. savigny, Echinothrix calamaris, Holothuria atra, and Synapta maculata. The first seven species belonging to the class Echinoidea while the latter two belong to the class Holothuroidea.

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