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Rachmat Hidayat
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dr.rachmat.hidayat@gmail.com
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dr.rachmat.hidayat@gmail.com
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INDONESIA
Bioscientia Medicina : Journal of Biomedicine and Translational Research
Published by Universitas Sriwijaya
ISSN : -     EISSN : 25980580     DOI : -
Core Subject : Health, Science,
BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to medical sciences, Traditional Herb, genetics, immunology, environmental health, toxicology, bioinformatics and biotechnology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.
Arjuna Subject : Kedokteran - Anatomi
Articles 1,143 Documents
Efficacy of cinnamon (Cinnamomum burmannii) extract to decrease serum creatinine in acute kidney injury induced male wistar rats Evi Lusiana; Nia Savitri Tamzil; Desi Oktarina
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i4.101

Abstract

Background Kidney vital function as a regulator of blood volume and chemical composition to excrete solute and water selectively. Acute kidney injury (AKI) is a sudden decline in kidney function which is temporary, is marked by an increase in serum creatinine levels and decreased urine output. Objective This study aims to determine the effectiveness of cinnamon extract in acute kidney injury induced male wistar rats. Method An experimental in vivo with pre-post control group design was conducted in twenty-five wistar strain white rats that were divided into 5 treatment groups that received methylprednisolone as a positive control, aquades, and different dose of cinnamon extracts (50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW). The rat model of acute kidney injury was prepared by the method of unilateral ureteral obstruction (UUO). The effectiveness of cinnamon extract was carried out by creatinine levels checked using ELISA and analyzed by ANOVA. Results The extract of cinnamon can lower serum creatinine levels were significantly (p <0.05). A dose of 100mg / KgBW is an effective dose in AKI compare to methylprednisolone. Conclusion Extract of cinnamon (Cinnamomum burmannii) corrected creatinine levels of acute kidney injury induced male wistar rats. Keywords: Acute kidney injury, cinnamon extract, creatinine
Association of Stress Level with Menstrual Disturbance Among Female Students in Medical Faculty Sriwijaya University Fildzah Hashifah Taufiq; Herry Hasnawi; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.82

Abstract

Association Of Stress Level With Menstrual Cycle Disturbance Among Female Students In Medical Faculty Of Sriwijaya University. Menstruation occurs regularly every month will form a menstrual cycle. Normal menstrual cycles in women range from 21-35 days. However, the menstrual cycle is not always normal, many women experience menstrual disturbance. Menstrual cycle disturbance are caused by several factors, which is stress. This study aims to analyze the association of stress level with menstrual cycle disturbance among female students in Medical Faculty of Sriwijaya University. This study is analytical cross-sectional study with primary data from menstrual cycle, stress ISMA, personality type DSM V questionnaires.. In this study, 503 samples are fulfilled inclusion criteria. Among 503 subjects, 59 (11.7%) female students experienced polymenorrhea, 391 (77.7%) female students had normal menstrual cycles, 53 (10.6%) female students experienced olygomenorrhea. Chi Square test showed that there was an association between stress level and menstrual cycle disturbance. There is a significant association between between stress level and menstrual cycle disturbance among female students in Medical Faculty of Sriwijaya University. Keywords: Stress level, Menstrual cycle disturbance.
Screening A Trinucleotide Repeat Expansion: How precise PCR can be? Ziske Maritska; Baharudin Baharudin; Ardy Santosa; Ching Leng Kee; Tan Yue Ming; Sultana MH Faradz
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 3 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i3.94

Abstract

ABSTRACT Background. Trinucleotide Repeat Expansion (TRE) in human DNA could lead to various diseases. An expanded CAG repeat (>31 or 37 repeats, depends on the ethnicity) in Androgen Receptor gene is suggested to be associated with the occurrence of isolated hypospadias. In an effort to identify the exact numbers of repeats, sequencing has been the most favored method to be conducted despite its cost. Objective. This study wished to investigate the possibilities of using Polymerase Chain Reaction (PCR) method to screen expanded repeats in isolated hypospadias, as one of the TRE diseases. Materials and Methods. Numbers of CAG repeat in twelve hypospadias patients and one normal male was first predicted from the visualization of PCR products in 3% agarose gel electrophoreses with 20 bp ladder marker before it was finally sequenced. Results. Two samples gave the same exact result, while the rest showed a range of 1-11 bp differences. Statistically, there was a significant difference between the mean of CAG repeats from PCR method (M=26.1667, SD=6.71272) and the mean of CAG repeats from sequencing (M=23.75, SD=5.70685); t(11)= 4.570, p=0.001. Furthermore, the sensitivity of PCR was 100% and the specificity was 83.33%. Conclusion. It can be concluded that PCR method could be used as a screening method in identifying TRE with large numbers of repeats. However, PCR in TRE disease with small numbers of expanded repeats needs to be followed by sequencing in order to obtain the exact numbers of repeats. Keywords: Trinucleotide Repeat Expansion, Polymerase Chain Reaction, Sequencing, Isolated Hypospadias
The Anticancer Activity of Srikaya Leaves Fraction (Annona squamosa L.): An In Vitro Study Makbruri Amin; Irsan Saleh; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i4.102

Abstract

Abstract Introduction: Anticancer drugs are aimed primarily at inhibiting the growth and proliferation of cancer cells. Srikaya leaves (Annona squamosa L.) had been proven to possess various therapeutic effects and potential to be developed as anticancer drugs due to its cytotoxic activity. Aim of study: This study aimed to assess the anticancer activity of srikaya leaves (Annona squamosa L.) fraction. Methods: Methanol fraction of srikaya leaves were obtained at concentrations of 500; 250; 125; 62.5; 31.25 µg/ml. Srikaya methanol fraction and cisplatin as control were given to a plate that was sealed with T47D cells for MTT assay. Identification of compounds in the methanol fraction of srikaya leaves was performed with thin layer chromatography (TLC). Data were collected in the form of absorbance value and half-maximal inhibitory concentration (IC50) value was determined ​​by linear regression. Data analysis was carried out with paired T test, unpaired T test, and ANOVA. Results: Average percentage of T47D cells viability increased with the decrease in the concentration of srikaya methanol fraction. Obtained IC50 value was 174.25 µg/ml which was quite active and potential to be developed as an anticancer drug. Methanol fraction of srikaya leaves contained secondary terpenoid metabolites, steroids, phenols, flavonoids, alkaloids and tannins. Flavonoid was the dominant metabolites in phytochemical tests and believed to play a major role in cytotoxic activity of srikaya leaves. Conclusion: Methanol fraction of srikaya leaves possessed the cytotoxic effect on T47D cancer cell line through the role of flavonoid metabolites. Keywords: srikaya, Annona squamosa, anticancer, T47D cells
Antidepresant effects of Cinnamon (Cinnamomum burmannii) extract in depressed induced rats using 3-minutes Tail Suspension method Nita Parisa; Mayasari Mayasari; Nia Savitri Tamzil; Bintang Arroyantri; Ziske Maritska
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 3 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i3.91

Abstract

Abstract Background. The increasing prevalence of depression gives rise to challenges in not only elucidating its diverse causes, but also in finding an effective treatment. One of the factors linked to depression is the imbalance of serotonin, norepinephrine, and dopamine neurotransmitters. Cinnamon (Cinnamomum burmannii) as one of the world’s wellknown cooking ingredients is believed to be able to regulate the neurotransmitters imbalance with the help of terpenoids and flavonoid polyphenols as one of its content. Objective. This study aims to determine the effectiveness of cinnamon extract as an antidepressant in depressed induced animal model. Methods. An experimental in vivo with pre-post control group design was conducted in twenty five Wistar strain white rats that were divided into 5 treatment groups that received fluoxetine as positive control, aquades, and different dose of cinnamon extracts (50 mg/kgBW, 100 mg/kgBW, and 200 mg/kgBW). Depression induction method used was 3-minute Tail Suspension Test, done for 14 days. The antidepressant effectiveness test was carried out by calculating the immobility time duration with Forced Swimming Test method and was further analyzed using one-way ANOVA test. Results. One-way ANOVA test results showed that there were differences in the mean duration of immobility time between treatment groups after being given cinnamon extract (p value = 0,000). Groups that were given 100 mg/kgBW cinnamon extract and 200 mg /kgBW showed a p value>0.05 when compared with positive control group receiving Fluoxetine although displayed a similar reduced immobility time. Conclusion. Cinnamon (Cinnamomum burmannii) extract showed a promising potential as an effective antidepressant tested in animal model. Keywords: cinnamon, extract, depression, immobility time, rat
Identification of Plasmodium Falciparum Merozoit Surface Protein-1 (PFMSP-1) Gene From Malaria Patients in Hanura Area, Lampung, Indonesia Ade Triajayanti; Jhons Fatriyadi Suwandi; Betta Kurniawan; Nurul Utami
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i4.97

Abstract

Introduction: Malaria is a major health problem not only in Indonesia, but also the world. Incidence of anti malarial drug resistance has been found, and one of the possible cause is genetic factors. The changes that occur in the Plasmodium falciparum gene cause genetic variations that lead to resistance for treatment. There is a gene with high polymorphism that can be used as a gene marker of genetic variation in Plasmodium falciparum. Aim of Study: This study aim to identification of plasmodium falciparum merozoit surface protein-1 (PFMSP-1) gene from malaria patients in Puskesmas Hanura, Pesawaran, Lampung. Methods: Descriptive method with morbidity survey approach is used in this study. There are total 23 biological material stored that had been taken in 2016 from malaria patient in Puskesmas Hanura, Pesawaran, Lampung, by consecutive sampling. Identification of genetic variance of the Plasmodium falciparum gene was performed by nested PCR at Medical Faculty Biomolecular Laboratory of Lampung University. The results of this study were processed using a computer software. Results: There are 23 samples that amplified following nested PCR for Plasmodium falciparum Merozoit Surface Protein-1 (PFMSP-1) gene target. In all samples studied, there are PFMSP-1 gene with six variations base pair length range from 200 to 2000 bp. Conclusions: this study has successfully amplified PFMSP-1 gene from individual’s blood sample infected by Malaria in Puskesmas Hanura work region, Pesawaran, Lampung.
Birth Anthropometry Characteristics of Hypospadias Patients in Palembang, Indonesia ziske maritska; Savero Evan Abisha Evan Abisha; Triwani Triwani
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 3 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i3.92

Abstract

ABSTRACT. Background. Hypospadias is one of the most common urogenital anomaly in males. The etiology is poorly understood, but is believed to be multifactorial where Intra Uterine Growth Restriction (IUGR) is one of the suspected risk factors, leading to low birth weight and low birth length in affected patients. Objective. This study aims to identify the birth anthropometry characteristics of hypospadias patients who visited RSUP. Dr. Mohammad Hoesin Palembang. Materials and Methods. This was an observational descriptive study with cross sectional design. This study included hypospadias patients with detailed birth characteristics in RSUP Dr. Mohammad Hoesin Palembang during the period of 2012-2015. Results. Out of 106 hypospadias patients, only 19 (17.92%) of them presented with detailed birth characteristics. There were 12 (63,2%) patients with low birth weight and 7 (36,8%) patients with normal birth weight. There were 10 (52,6%) patients with low birth length and 9 (47,4%) patients with normal birth length. The mean birth weight is 2442 grams and the mean birth length is 46,9 centimeters. Conclusion. The majority of hypospadias patients in this study showed a low birth weight and low birth length, signalling an accordance to the existing theory. Keywords: Hypospadias, birth anthropometry, characteristics
The Comparison of High Flow Nasal Cannula Oxygen and Conventional Oxygen Therapy on Early Effect Hemodynamic Indicators in Post – Upper Abdominal Surgery Patients: A Pilot Study Luki Sumaratih; Rudyanto Soedono; Dita Aditianingsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 4 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i4.99

Abstract

Background The effect of high-flow nasal cannula (HFNC) on hemodynamic data of the patients is still unknown. This was a pilot study to evaluate the effect of HFNC and conventional oxygen therapy (COT) on post-upper abdominal surgery. Methods This was an open-label randomized controlled trial (RCT) at tertiary hospital between February-June 2019. Inclusion criteria were ASA III, aged 18-65 years, BMI 18,5-29 kg/m2. Exclusion criteria were patients with unstable hemodynamic with vasopressors, general oedema, and uncooperative. Thirty patients were recruited and divided into HFNC group (n=15) and COT group (n=15). Hemodynamic parameters were recorded using the bedside monitor (heart rate, respiratory rate, and mean arterial pressure) as well as the electrical cardiometry using ICON® measurements (stroke volume index, cardiac index and systemic vascular resistance index); laboratory parameters were ScVO2 and lactate serum. Data were collected at 0, 30 and 60 minutes post-extubation. Results The General Linear Model (GLM) tests on clinical parameters (HR, RR and MAP) were P= 0.20; P=0.72; P = 0.18, ICON® parameters (SVI, CI and SVRI) were P= 0.02;P =0.64; P =0.64 meanwhile ScVO2 and lactate were P = 0.35 and P = 0.22. Despite those results, at some period of measurements there were a tendency of a better outcomes of most parameters for the HFNC group. Conclusion According to the results, the only statistically significant outcome was SVI (P =0.02) after the analysis between those two groups. However, the HFNC group showed a clinically improvement of the other outcomes among post-upper abdominal surgery patients. Keywords High flow nasal cannula, conventional oxygen therapy, haemodynamic, abdominal surgery
N-Acetyl-Glucosamidase as Biomarker for Acute Kidney Injury Aulia Annisa Rizki; Zulkifli Zulkifli; Agustina Br Haloho; Zen Hafy
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i4.56

Abstract

Background : The estimated inci­dence of Acute Kidney Injury (AKI) is two to three cases per 1,000 patients. Seven percent of hospitalized patients and about two-thirds of patients in intensive care units develop acute kidney injury and the mortal­ity rates range between 25 and 80 percent. Disruption in epithelial brush border of proximal tubular cell causes N-Acetyl-β-D-Glucosaminidase (NAG) to be released to the urine and the amount of enzyme could be directly correlated with tubular disruption. Objective : The aims of this research is to determine the sensitivity and specificity of NAG urine examination as an early biomarker for acute kidney injury. Methods : There’s 66 subjects who met the inclusion criteria. All the subject were checked for the NAG urine level with Cloud Clone kit and creatinine serum were also checked 48 hours after admission. Results : The results showed in the cut-off point of 7.98 Ng/mL, urine NAG has a sensitivity of 68.6% and specificity 77.4%, positive predictive value 77.42%, negative predictive value 68.57 % and accuracy of 72.73% Conclusions : The result shows that urine NAG examination is more sensitive and specific as an early biomarker for Acute Kidney Injury compared to creatinine serum. Key words : Acute Kidney Injury, N-Acetyl-β-D-Glucosaminidase , sensitivity, spesificity
The Effect of Ophiocephalus striatus Extract on Quantitative Measurement of Biceps Brachii and Rectus Femoris in Patients with Mechanical Ventilation: A Randomised Control Trial Hunter Design Nainggolan; Eddy Harijanto; Adhrie Sugiarto; Inge Permadhi; Thariqah Salamah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 4 No. 1 (2020): Bioscientia Medicina: Journal of Biomedicine and Translational Research
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v4i1.104

Abstract

Introduction A freshwater fish Ophiocephalus striatus (OS) or known locally to Indonesian as haruan, has potentials to retain musclemass of critically ill patients following its high content of amino acids, fatty acids, minerals, and vitamins. This study was conducted to investigate the effect of OS on quantitative measurements of biceps brachii and rectus femoris in patients with mechanical ventilation. Methods This was a double-blinded randomized controlled trial at tertiary hospital between July- September 2019. The study included patients aged 18-65 years old with mechanical ventilation whereas patients with chronic kidney disease, paraparesis, or neuromuscular disorders were excluded. Forty-two patients were divided into extract group (n=19) who received 15 g of OS extract daily and control group (n=23) who received none. Quantitative measurements of both muscles were determined by measuring cross sectional area (CSA) using ultrasonography by one radiologist. Data were collected at 1st and 7th day, while the intervention was administered in-between. Independent sample T-test, Mann-Whitney test, and multivariate study using general linear model (GLM) were used for statistical analysis. Results The changes of CSA on biceps brachii and rectus femoris muscles from before to after intervention between extract group compared to control group were 5,30 ± 2,41 to 6,09 ± 1,81 vs. 5,16 ± 2,50 to 4,16 ± 1,94 (p=0.002) and 2,45 (1,10 – 5,90) to 3,47 (1,13 – 8,18) vs 3,85 (1,09 – 9,06) to 2,78 (1,06 – 7,27) (p=0.230), respectively. Multivariate analysis showed significant differences between extract and control group for both muscles with p value of <0.001 and 0.001 respectively. Conclusion Supplementation of Ophiocephalus striatus extract was associated with a significant increase of biceps brachii and rectus femoris CSA in patients with mechanical ventilation. Keywords: Ophiocephalus striatus, ultrasonography, cross sectional area, biceps brachii muscle, rectus femoris muscle

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