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Bioscientia Medicina : Journal of Biomedicine and Translational Research
Published by Universitas Sriwijaya
ISSN : -     EISSN : 25980580     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to medical sciences, Traditional Herb, genetics, immunology, environmental health, toxicology, bioinformatics and biotechnology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.
Arjuna Subject : Kedokteran - Anatomi
Articles 1,143 Documents
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Efficacy of SEBCLAIRA® Cream for Moderate Seborrhoeic Dermatitis on The Face as An Adjuvant Therapy (Randomised Clinical Trial, Double Blind) Inda Astri Aryani; Yulia Farida Yahya; Nina Roiana; Radema A Pranata
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.47

Abstract

Background: Seborrhoeic dermatitis (SD) is a chronic papulosquamous inflammatory disease which resistant to medical treatment. Various treatment such as topical corticosteroid, antifungal and calcineurin inhibitor has been widely practiced and gives varying results Objective: Our objective was to compare the efficacy of Sebclaira® and topical hydrocortisone 2,5% for management of moderate SD on the face Methods: A randomised clinical trial, controlled, double blind study was performed for four weeks. We assessed the efficacy and side effects of these topical treatment. The severity of SD was evaluated using Seborrhea Area Severity Index-Facial (SASI-F) score. The severity of pruritus was evaluated using Visual Analogue Scale. Demographic characteristics, baseline SASI-F and VAS were recorded in the medical record. Results: A 34 patients (14 males, 20 females) with moderate SD on the face completed the four weeks study. The mean of SASI T2 and VAS score of the Sebclaira® group was significantly lower than Hydrocortisone 2.5% group with p = 0.000 and p = 0.000 respectively. Tolerance between Sebclaira® and Hydrocortisone 2.5% showed insignificant results (p = 1.000) Conclusions: The longer application of Sebclaira® was significantly more effective to improve moderate SD
The Development of Prototype Polyclonal Antibody of Receptor Advanced Glycation of Endproducts (RAGE) Mgs Irsan Saleh; Nita Parisa; Ziske Maritska
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.16

Abstract

Abstract Background Receptor for advanced glycation endproducts (RAGE) is a transmembrane protein that belongs to the immunoglobulin superfamily. As its name implicates, it can bind to advanced glycation endproducts, the resulting product of non-enzymatic glycosylation, and it also has the ability to interact with multiple ligands having common motifs as a so-called multi-ligand receptor. The ligands include high-mobility group protein (B)1 (HMGB1), S-100 calcium-binding protein, amyloid-β-protein, Mac-1, and phosphatidylserine. Interaction between RAGE and its ligands activates various cellular processes, including inflammation, proliferation, apoptosis, autophagy, and migration. Methods The proccess of isolation protein of RAGE was initiated with extraction protein and purification of RAGE protein. After that, the immunization of Rats was be done to produce Anti-RAGE. The confirmation of Anti RAGE was be done by SDS PAGE and Immunobloting. Results The production of Anti RAGE was enough pure compared by Anti RAGE commercial. Anti RAGE was protein that have molecular weight around 35 kD. Conclusion The methods that used in this study effective to develop production of anti-RAGE. Keywords : Anti RAGE – Isolation – Production Antibody
The Relationships between Neutrophil/Lymphocyte Ratio and Child-Turcotte-Pugh Classification in Assessing Severity of Liver Cirrhosis Griselda Griselda; Kemas Ya'Kub Rahadiyanto; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i1.80

Abstract

Background Liver cirrhosis is a disease with high morbidity and mortality in Indonesia. The Child-Turcotte-Pugh classification is a noninvasive parameter to assess severity of liver cirrhosis. Meanwhile, the neutrophil/lymphocyte ratio can reflect person's immunity to liver cirrhosis. This study was conducted to determine the agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Methods Analytical observational study with Cohen’s Kappa agreement test was conducted in 86 liver cirrhosis patients. Data was retrieved by observing the medical records. Neutrophil count, lymphocyte count, hepatic encephalopathy, ascites, albumin, bilirubin, and INR were all recorded. Documented variables were analyzed by the Cohen’s Kappa agreement test. Results In Cohen’s Kappa agreement test, there was moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in all samples (κ = 0.591 and p <0.001). Agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification based on age and gender was found to be κ = 0.777 at age ≤45 years, κ = 0.532 at age >45 years, κ = 0.682 in male, and κ = 0.445 in female (p <0.001). The highest agreement is the substantial agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification obtained at age ≤45 years (κ = 0.777 and p <0.001) and male (κ = 0.682 and p <0.001). Conclusion There is moderate agreement between neutrophil/lymphocyte ratio and Child-Turcotte-Pugh classification in assessing severity of liver cirrhosis. Substantial agreement obtained at age ≤45 years and male gender. Keywords: Neutrophil/Lymphocyte Ratio, Child-Turcotte-Pugh, Liver Cirrhosis
The Risk Factors of Anorectal Malformation Patients Mortality in Palembang Muhammad Bima; Shalita Dastamuar; Rachmat Hidayat
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 3 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i3.59

Abstract

Anorectal malformations are serious anal and rectal disorders ranging from stenosis to anorectal agenesis. The incidence of mortality in cases of anorectal malformations is influenced by many factors, including the type of anorectal malformation, associated congenital anomalies, sepsis, and prematurity as investigated in this study. The aim of this study was to identify the risk factor of mortality on anorectal malformation patients. This study is an observational analytic study with cross sectional study design. Secondary data collection was obtained from eighty-two medical records of anorectal malformation patients in Dr. Mohammad Hoesin Hospital Palembang from January 2015 to June 2017 that meets the inclusion criteria. From eighty-two cases of anorectal malformations found nineteen cases of mortality (23.2%). There were four risk factors identified, anorectal malformation type, associated congenital anomalies, sepsis, and prematurity. Two risk factors found significantly affect the mortality on anorectal malformation patients, anorectal malformation type (p value = 0.036) and sepsis (p value = 0.049) from four risk factors studied. Type of anorectal malformation and sepsis have a significant effect on the mortality of patients with anorectal malformations, but there is no effect on the mortality of the patients with assciated congenital anomalies and prematurity. Keywords: Anorectal malformation, congenital abnormalities, sepsis, prematurity
Granulocyte Colony Stimulating Factor (G-CSF) Quantitative Analysis In Plasmodium Vivax Infected Malaria Patients Experiencing Trombocytopenia Gita Dwi Prasasty; R. A. Leni Septiana; Chairil Anwar; Dwi Handayani
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.43

Abstract

Abstract Introduction : P. Vivax has been refered as pathological factor underlying increasing prevalences of haematological abnormality including anemia and thrombocytopenia. Through the day, exact mechanism of thrombocytopenia in malaria infection has yet come to a conclusion, several hypothesis are still in considered, phagocytosis and platelets aggregation remain the major disscussion topics. G-CSF, cytocine with elevated serum quantity in P. Vivax infections, were responsible in increasing phagoctytosis and conducting direct effect on platelets aggregation using adequate ADP. Increasing number of ADP in malaria cases were correlated with erythrocyte haemolitic, leading to increasing platelets aggregation. Although numerous hypothesis has been compelled, only a-few research publication has been made corresponding to G-CSF serum level on malaria P. Vivax infection and its correlations to thrombocytopenic events. Aim of study : To analyze the relations between G-CSF serum levels and parasite numbers towards platelets profile in infected malaria P. Vivax patients. Methods : Study design using Prospective analysis. Thirty six patients with single infection of Malaria Vivax in Puskesmas Sukamaju (Primary Heath Care Centre) and Puskesmas Kota Karang were assessed for G-CSF plasma levels, platelet counts, and MPV. Data analysis were conducted using Spearman correlation methods with SPSS. Results : Study results showing significant correlation between G-CSF serum levels towards Platelet conts (R = -0,397(p = 0,016)), without significant correlation between G-CSF and MPV value (p = 0,874) Conclusion : G-CSF serum levels were related with thrombocytopenia, with no correlation towards MPV. Keyword: G-CSF, P. vivax, Thrombocytopenia, MPV.
A Hospital-Based Study: Interventions for Patients with Disorders of Sex Development (DSD) in National Reference Hospital, South Sumatera Region, Indonesia Ziske Maritska; Bintang Arroyantri Prananjaya; Andhika Diaz Maulana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 3 No. 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v3i2.86

Abstract

ABSTRACT Background. Disorders of Sex Development (DSD) is a term used to describe a congenital condition where sex chromosomes, gonads, and or anatomy of a person are atypical. It is estimated to affect 1:4,500 people worlwide with varied clinical findings, hence the integrated and diverse interventions. Objective. This study wished to observe the many interventions DSD patients received in Dr. Mohammad Hoesin Hospital as one of the national reference hospitals in Indonesia for South Sumatera region. Methods. Medical records of 173 patients with clinical findings in accordance with DSD spectrum during 2013-2017 was observed in order to identify both surgical and medical interventions applied. Results. Sixty two patients were diagnosed of having hypospadias (35.84%), making it the most common clinical finding among DSD patients in this hospital. Surgical intervention was the most favored intervention, with 155 patients (89.6%) underwent various surgeries, ranging from urethroplasty to vaginoplasty and many more. Five patients(2.9%) were treated with either hydrocortisone and florinef, or both. One patient diagnosed with Congenital Adrenal Hyperplasia (CAH) had both surgical and medical intervention. Conclusion. DSD gives a wide phenotypic spectrum, making its management both challenging and complicated. Surgical, medical, and or a combination of both are the indispensable interventions when dealing with DSD patients. Study focusing on psychological intervention for DSD patients is further needed. Keyword: Disorders of Sex Development, DSD, surgical, medical, intervention.
The Efficacy of Temu Putih Fraction (Curcuma Zedoaria (Berg) Roscoe) Related Quality and Quantity of Spermatozoa in Male Wistar Rats Tiara Fatrin; Salni Salni; Sri Nita; Rachmat Hidayat; Triwani Triwani; Joko Marwoto; Ziske Maritska; Mgs Irsan Saleh; Nita Parisa; Nia savitri Tamzil; Evi Lusiana; Ayeshah Agusta Rosdah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.12

Abstract

Background Male participation in KB is still relatively low when compared to the participationof women. Researchers have to do research to find the contraception drug. Temu putih (Curcuma Zedoaria (Berg) Roscoe) is one of traditional herb that used as antifertility. Aim of Study Aim of this study to examine change in the amount, motility, morphology, and viability spermatozoa male rats (rattus norvegicus) due to temu putih fraction suplementation. Methods This study was an experimental study using a completely randomized design (CRD), post test with control group design. The sample in this study was 30 male rats, 10 weeks old, weight 150-200 gram. Rats were given temu putih fraction (n hexan, etylacetate and methanol-water) at dose of 300 mg/kgBB/day for 48 days. Temu putih was extracted by ethanol and did fractionation by liquid-liquid methods. The results of this study were assayed by SPSS 18. Results The amount, motility, morphology and viability of spermatozoa in the group of metanol fraction of water decreased compared with the control group (p= 0,000), motilitas of spermatozoa in the group of metanol water fraction decreased compared with the control group. Conclusion Temu putih fraction can reduce the amount, motility, morphology, and viability of spermatozoa in male rats. Keywords: Fraction, Temu Putih, Amount of spermatozoa, Motility of spermatozoa, Viability Of Spermatozoa
Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury Febri Jaya Gunawan; F Heru Irwanto; Andi Miarta; Theodorus Theodorus
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i4.58

Abstract

Background : Incidence of AKI in intensive care unit patients reach 60-70%, and the mortality rate is about 60%. IL-18 is a pro-inflammatory cytokine which increased in endogenous inflammation process. Studies in human showed that IL-18 concentration increased prior to AKI. Objective : The aims of this research is to determine the sensitivity and specificity of Interleukin-18 urine examination as an early biomarker for acute kidney injury. Methods : There’re total of 66 subjects who met the inclusion criteria. All the subject were checked for the IL-18 urine level with Cloud Clone kit and creatinine serum were also checked 48 hours after admission. Results :The results showed in the cut-off point of 411.25 Pg/mL , urine IL-18 has a sensitivity of 54.3 % and specificity 83.9%, positive predictive value 79.17%, negative predictive value 61.9% and accuracy of 68.18% Conclusions : Our result suggest that IL-18 urine holds a promise as an early biomarker of AKI and more sensitive and specific as an early biomarker for AKI compared to creatinine serum. Key words : Acute Kidney Injury, Interleukin-18, sensitivity, spesificity
Risk Factors for Stunting Children Aged 6-59 Months In Pulau Panggung District, South Sumatera, Indonesia Prihatina Anjela; Rachmat Hidayat; Debby Handayanti Harahap
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.48

Abstract

Background :. Stunting among children is one of the major health problems in developing countries. Globally, 45% of deaths among children are caused by malnutrition, meanwhile stunting, as one of the factors, plays an important role. Children who experience stunting can not achieve optimal growth and cognitive potential. Hence, that will have an impact on future productivity. Muara Enim district is included in one district with stunting prevalence, which is above 20%, i.e. 26.9%. This study was to identify the risk factors of stunting children in Pulau Panggung district, Muara Enim, South Sumatera. Methods: This study applied observational analytic research using cross sectional design. It was conducted on August to October 2017 at public health center in Pulau Panggung district, Muara Enim. There were 83 children who fullfilled the inclusion criteria. The relationship between risk factors and stunting was analyzed using Chi square test and Logistic Regression test was applied to identify the risk factor that play the most role to stunting. Results: 49,4% out of 83 children suffer from stunting, with a mean age of 29,37±13,69 months where the number of children stunting with male gender is 53.7% while female gender is 46.3%. Chi square analysis showed that there was a significant relationship among birth weight, birth length, mother's occupation, history of infection, exclussive breastfeeding and stunting (p< 0.05) but no significant relationship was found among sex, mother's education, maternal height and income with stunting (p> 0.05). Multivariate Logistic Regression test showed that mother's occupation and history of infection are the risk factors that contibute the most to stunting among children aged 6-59 months in Pulau Panggung district. The risk of stunting among working mothers is higher 5 times than unworking mothers and children with infection history have risk of stunting 3 times compared to children without infection history. Conclusion: Mother's occupation and infection history are risk factors that contribute the most to stunting among children at Pulau Panggung district, Muara Enim, South Sumatera.
Identification of Lactic Acid Bacteria Isolate From Fermentation Food Bekasam Ardesy Melizah; Syarif Husin; Syifa Alkaf
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.34

Abstract

Bekasam is a local food from South Sumatera Indonesia, which is obtained through fermentation of fish. Previous study has shown that fermented food contained Lactic Acid Bacteria (LAB) bacteria, such as Lactobacillus plantarum, Lactobacillus sp, Pediococus sp and Weissella sp. The study was conducted to identify isolate LAB from bekasam. Bekasam contained fish, rice, and salt with a certain ratio. Further isolation of isolated LAB isolate, then performed PCR for bacterial analysis. Isolate 1 showed alleged bacteria Staphylococcus sp, non pathogen, while isolate 2 leads to Lactobacillus sp. The PCR results show the suitability of the bands formed between the Lactobacillus standard and the isolates. Keywords: bacteria, lactobacillus, acid

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