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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
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Search results for , issue "Vol. 8 No. 1 (2021)" : 10 Documents clear
THE STATUS QUALITY WATER OF CIRCULATION COOLING WATER IN THE MELTING SCRAP FACTORY AND RECYCLING Fian Surya Alif; Tugiyono Tugiyono; Agus Setiawan; Gregorius Nugroho Susanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.158

Abstract

Recycling metal scrap is an effort to save The earth from pollution, meanwhile in the process of recycling metal scrap usually it took a lot of water in the process to cooling it from such a high temperature. The purpose of this study was to identified water quality from the water in the reservoir and the water quality of the river that indicated receiving overflow from reservoir water when in the rainy season. The study was conducted from October to November 2020 at the Melting Scrap Factory and the river around the industrial zone. The study was conducted by taking a water sample for analysis in the spot (in-situ) and in the laboratory (ex-situ). The water sample was taken by a field technician laboratory. The result from laboratory analysis counted to indicate the water quality using Pollution Index methods (PI).and then in order to detect pollution from the fish that lived inside the pond using nutrition value coefficient (NVC). After the analysis data was counted the result showed that the water-saving pond had 3,20 values for pollution index(PI) and the river had 3,71 values for pollution index(PI), both of the results indicated that status water quality was classified as lightly polluted if it referred to the policy KEPMEN LH NO 11 Tahun 2003 for standard water quality class 3. Based on the data that has been counted for NVC the result was 1,84, it showed that the fish could live healthily but for further analysis laboratory on the fish meat, it showed that the fish meat was contained a high amount of a heavy metal compound such as iron (Fe) which had value over the standard (1953 mg/kg), from that value it showed how polluted the fishes in the reservoir. The fish should not be eaten by humans because of the high amount of heavy metal compound
GROWTH OF PURPLE CABBAGE (Brassica oleracea L. var. capitate f. rubra) ON THE ADMINISTRATION OF POLYETHYLENE GLYCOL 6000 (PEG 6000) Chandra Aria Wicaksono; Tundjung Tripeni Handayani; Martha Lulus Lande; Zulkifli Zulkifli
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.161

Abstract

Purple cabbage plants require an adequate supply of water in the process of growing. So that if there is a lack of water it can result in stressful conditions then the growth of purple cabbage is disrupted and can even cause death. The purpose of this study was to determine the tolerance limits of purple cabbage to PEG 6000, and to determine the effect of PEG 6000 on the growth of purple cabbage as a simulation of drought stress. This research was conducted using a completely randomized design with the main factor being PEG 6000 with 5 concentration levels: 0 % w/v, 10% w/v, 20% w/v, 30% w/v and 40% w/v with 5 replications. As parameters are plant height, length, width, and number of leaves, also chlorophyll content. Then analyzed with the Levene test, one way ANOVA and the Tukey HSD test at 5% level. Based on the results of the research, it can be concluded that giving PEG 6000 shows that the tolerance limit for purple cabbage plants to PEG 6000 treatment is at a concentration of 10%, then giving PEG 6000 gives a negative response to the growth of purple cabbage which is indicated by a decrease in plant height, leaf length, leaf width, number of leaf, also chlorophyll a, b, and total chlorophyll content.
THE INHIBITION OF RED CHILIES RIPENING PROCESS AT FRESH WEIGHT AND TOTAL CARBOHYDRATE CONTENT BY RED LIGHT Martha Lulus Lande; Salman Farisi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.162

Abstract

The research was conducted in a factorial experiment with factor A: far-red light treatment with 2 levels: control (without far-red light), and treatment (far-red light). Factor B was a time of observation with 3 levels: 4, 6, 8 days after wrapping. Wrapping each detached fruit in a combination of four layers of blue-colored plastic, one layer of green-colored plastic, and two layers of red-colored plastic resulted in a red light treatment. Transmission measurement of that plastic combined with a spectrophotometer at wavelength 700-735 nm was 75%. To deter fresh weight, each fruit was weighted with an analytical balance. Total soluble carbohydrate content was determined by the phenol-sulfuric method and counted based on the glucose standard curve (mg/gram tissue). Analysis of variance was conducted at a 5% significant level, and if the interaction was significant, then continued with simple effect determination at a 5% significant level by the F test. The fresh weight average of the soluble carbohydrate content of treated fruits was higher than the control. The fresh weight of chili fruits was relatively constant during observation of both treated and controlled fruits. The total soluble carbohydrate content average relatively did not differ between treated and control fruits. Total soluble carbohydrate content was increased 6 days after treatment and decreased 8 days after treatment. The result of this research concluded that red light inhibited the ripening process of chili fruits by slowing the decrease of fresh weight.
MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.) Nadya Febri Harlifia; Bambang Irawan; Salman Farisi; Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.163

Abstract

Bamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.)
VEGETATIVE GROWTH OF TOMATO (Lycopersicum esculentum Mill.) INFLUENCED BY AERATED COMPOST TEA (ACT) FROM BROMELAIN LITTER INDUCED BY LIGNINOLITIC Trichoderma sp. Sela Habibu Rohmah; Bambang Irawan; Salman Farisi; Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.164

Abstract

The pineapple waste increased by the rise of production in pineapple, one of increasing pineapple waste is pineapple pulp (bromelain litter). The bromelain litter will be disadvantage for the environment, this matter could be solved by converting bromelain litter into compost. The composting process in this study used ligninolytic fungus (Trichoderma sp.). Composting technology which currently has rapidly developed is Aerated Compost Tea (ACT) or a derivate product of compost. The use of this study was to ensure the best incubation period of ACT bromelain litter which induced by Trichoderma sp. fungus on the growth of tomato (L. esculentum Mill.). The study was conducted using a completely randomized design (CRD) with 7 treatments and 3 replications, namely: P0= control (without ACT), P1= ACT bromelain 24 hours, P2= ACT bromelain 48 hours, P3= ACT bromelain 72 hours, P4= ACT bromelain and leaf litter 24 hours, P5= ACT bromelain and leaf litter 48 hours, and P6= ACT bromelain and leaf litter 72 hours. The variables observed were number of leaves and plant chlorophyll content. The data obtained were analyzed with ANOVA at the level of 5 % and tested for the Least Significant Difference (LSD) at the level of 5 %. The results of this study showed that ACT was induced by Trichoderma sp. fungus which effective for the vegetative growth of tomato (L. esculentum Mill.) is ACT bromelain litter with incubation period up to 72 hours (P3).
BROMELAIN WASTE TEA COMPOST INDUCED BY LIGNINOLYTIC INOCULUM OF Trichoderma sp. ON THE GROWTH OF LEAF NUMBER AND CHLOROPHYLL CONTENT OF CHILI (Capsicum annuum L.) Syafira Clarisa Huda; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.165

Abstract

Pineapple is one kind of fruit that contains a lot of nutrition.  The waste from residual pineapple processing is called bromelain.  Bromelain contains polymer complex in the form of organic sources from nature if it could be decomposed properly.  One way to exploited bromelain is by composting.  Composting is done with the help of fungi of Trichoderma sp. to speed up the decomposition process.  The resulting compost is aerated to increase the nutrients and form a further decomposition process in the soil.  The research was carried out from January till March 2021 in the Microbiology Laboratory Biology, Faculty of Mathematics and Science, University of Lampung.  The parameters of this research are the content of chlorophyll and the number of leaves.  This research used a completely Randomized Design with 7 treatments that are A1 (24 hours of mixed bromelain), A2 (48 hours of mixed bromelain), A3 (72 hours of mixed bromelain), A4 (24 hours of pure bromelain), A5 (48 hours of pure bromelain), A6 (72 hours of pure bromelain), and A7 (control).  Based on this research the value of the highest chlorophyll A on A6 treatment is 0,655.  A value of the highest on chlorophyll B on A6 treatment that 0,429.  The highest total chlorophyll on the A6 treatment is 1,048.  Based on this research the A4 treatment has the highest average number of leaves compared to other treatments.    
APPLICATION OF BROMELAIN LITTER SOLID COMPOST INDUCED BY LIGNINOLITIK Trichoderma sp. FUNGUS TOWARDS NUMBER OF LEAVES AND CHLOROPHYL CONTENT CHILI PLANTS (Capsicum annuum L.) Jihan Haura; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.166

Abstract

Piles of uncontrolled pineapple peel waste have a negative impact that will affect various aspects of life, either directly or indirectly. Therefore, pineapple peel waste that can no longer be processed can be used for making compost. The taste of pineapple can be degraded biologically with the help of enzymes that can be produced by microorganisms. One of the microorganisms that can support the decomposition process is a group of fungi. In this study, the fungus Trichoderma sp. (Bioggp 5) is a ligninolytic fungus. Trichoderma sp. is a saprophytic soil microorganism that attacks natural pathogenic fungi and is beneficial to plants. The purpose of this study was to determine the effect of solid bromelain compost inoculum of ligninolytic fungi (Trichoderma sp.) on leaf number and chlorophyll content of chili plants (Capsicum annum L.) and the best dose of compost that could be used. This study used a completely randomized design (CRD) with 7 treatments, namely T0 (control), T1 (1.4% pure bromelain), T2 (1.7% pure bromelain), T3 (2% pure bromelain), T4 (1, 4% bromelain + leaf litter), T5 (1.7% bromelain + leaf litter), T6 (2% bromelain + leaf litter). The parameters used were the number of leaves and chlorophyll content. And the results obtained from this study were the T3 dose (2% pure bromelain) was the best dose for the number of leaves, while the T2 dose (1.7% pure bromelain) was the best dose for chlorophyll content.
RESISTANCE OF RED CURLY CHILI (Capsicum annuum L.) SPROUTS TO FUSARIUM OXYSPORUM INFECTION FROM SEEDS INDUCED BY 0.2 mT Essy Dumayanti; Rochmah Agustrina; Wawan Abdullah Setiawan; Eti Ernawiati; Yulianty Yulianty; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.167

Abstract

Curly red chili (Capsicum annuum L.) is widely used as industrial raw material because it has a spicy taste. The increasing demand for chili is not balanced with the level of production. Chilies are susceptible to disease, one of which is fusarium wilt. The magnetic field affected the physical and chemical properties of water so that it increased peroxidase enzyme activity and plant metabolism. This research was aim to know the protective effect seeds induced by 0.2 mT Magnetic Fiel had given to red curly chili (Capsicum annuum L.) sprouts to Fusarium oxysporum infection. This study was arranged in a completely randomized design (CRD) with 5 treatments consisting of M0F0 (control), M7F0, M7F60, M15F0, M15F60. M0 is a seed not induced by a magnetic field; M7 is seed induced by magnetic field 7 minutes 48 seconds, M15 is seed induced by magnetic field 15 minutes 36 seconds, F0 is sprouted not infected with F. oxysporum and F60 is sprouts infected with F. oxysporum for 60 minutes. Each unit is repeated 5 times. The results of the ANOVA showed that exposure to a magnetic field had a significant effect on increasing plant height at 21, 28, and 35 days after planting (HST); the wet and dry weight of 7 days old plants; and the content of chlorophyll a, b, and total before flowering 21 days after planting. Overall, the 0.2 mT magnetic field treatment for 7 minutes 48 seconds tended to give better results to increase the growth of chili plants against F. oxysporum infection.
ANATOMIC CHARACTERISTICS OF THE STEM OF MANGO (Mangifera spp.) IN BANDAR LAMPUNG M. Ramdan Syahputra; Yulianty Yulianty; Martha Lulus Lande; Suratman Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.183

Abstract

Mango is one of the most widely grown plants in Indonesia. The characteristic that distinguishes one type of mango from another is to observe its anatomy. Anatomical research on the mango branch has not been done much, especially in Bandar Lampung. The purpose of this study was to determine the diameter and pith radius of the mango (Mangifera spp.) branch in Bandar Lampung City. This research was carried out from January to April 2021 at the Botanical Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This study uses a survey method with Simple Random Sampling in 20 sub-districts in Bandar Lampung City. Parameters measured included tracheal diameter, tracheal density, arrangement and type of pith radius, pith radius height, and pith radius width. Data analysis used descriptive analysis by explaining the data obtained in the form of tables and photos. The results showed that there were differences in the average size of the tracheal diameter, tracheal density, height, and width of pith radius of the five mango branch samples. The average diameter of the largest trachea is found in Golek and the smallest tracheal diameter is found in Kweni. The highest average tracheal density is found in Arumanis and the smallest tracheal density is found in Golek. The arrangement and type of pith radius of the fifth mango are uniseriate and heterocellular. The average height of the largest pith radius is owned by Indramayu and the smallest is owned by Golek. While the average width of the largest pith radius is owned by Golek and the smallest is owned by Gedong.   
VIABILITY AND PRODUCTION CALCIFYING BACTERIAL ENDOSPORE ON SAND-CEMENT CARRIER Apriliani Devinta; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.184

Abstract

Carbonatogenic bacteria have the ability to precipitate calcium carbonate (CaCO3) and many calcareous areas are found. Some of its species formed endosporas which resistant to harsh physical condition such as very alkaline pH. The objectives of this study were to obtain spore biomass and determine endosporas viability in tested carrier media such as sand-cement. The tested isolates were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosracina JA4. The production of endosporas was carried out on yeast urea broth with a temperature treatment of 70°C for 20 minutes. The formed endosporas were stored in carrier medium of sand, cement, and a mixture of cement sand. Spore viability was conducted using total plate count method. The results showed that all isolates were able to produce endosporas with the highest endosporas dry biomass is Bacillus AK4 which was 196 mg/L. After 2 hours of storage, endosporas were still able to grow by forming colonies on nutrient agar media.

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