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All Journal Biospecies Indonesian Journal of Forestry Research Jurnal Sumberdaya Alam dan Lingkungan Jurnal Riset Akuakultur Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Unri Conference Series: Community Engagement Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Jurnal Kesehatan Kusuma Husada JURNAL BIOLOGI PAPUA AMMA : Jurnal Pengabdian Masyarakat Indonesian Journal of Forestry Research
Salman Farisi
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Nursing Public Health

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PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG Sumardi Sumardi; Komang Rima; Salman Farisi; Endang Linirin Widiastuti
Indonesian Journal of Forestry Research Vol 8, No 1 (2021): Indonesian Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2021.8.1.73-82

Abstract

Intensive shrimp culture has encountered many problems, such as declining water quality through disease caused by pathogenic microbes, which affected mortality. This study aimed to determine any potential probiotic from Bacillus sp. collected from mangrove in East Lampung, which could be used to improve the cultured shrimps' proteolytic and probiotic activity. This is a descriptive research with sampling and data collection of bacteria from many samples of mangrove. Result shows 128 isolates Bacillus from which then it has arrived at five potential probiotic Bacillus sp. The study five Bacillus sp. has been isolated with potential properties for probiotic (KPP212, IP121, UJ131, UJ132, SB141). Each isolate has characteristics with proteolytic property, growth in a wide range of pH 4–10 and osmotic stress (0–6% NaCl), non-pathogenic, ability for glucose fermentation, non-motile, and has negative catalase activity. The five potential Bacillus sp. can be used as probiotics for shrimp farming.
Pembuatan Sabun Cuci Dari Minyak Jelantah Sebagai Upaya Mengurangi Limbah Rumah Tangga Kusuma Handayani; M Kanedi; Salman Farisi; Wawan A. Setiawan
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 2 No. 1 (2021)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v2i1.25

Abstract

Minyak goreng bekas yang sudah berwarna coklat kehitaman sebaiknya tidak digunakan lagi karena bersifat karsinogenik. Minyak goreng yang telah dipakai berulang kali biasa disebut dengan minyak jelantah. Biasanya minyak jelantah (mijel) akan dibuang oleh ibu-ibu secara sembarangan sehingga menyebabkan minyak tersebut menjadi limbah di lingkungan sekitar. Kegiatan ini bertujuan untuk mengedukasi ibu-ibu mengenai bahaya penggunaan mijel secara berulang kali serta membantu memberikan solusi agar mijel dapat dimanfaatkan kembali dalam bentuk lain, salah satunya dibuat sabun cuci. Peserta kegiatan ini adalah ibu-ibu PKK di Kelurahan Labuan Ratu Raya Bandar Lampung, sebanyak 13 orang. Kegiatan ini terdiri dari, diskusi, demonstrasi dan praktik langsung. Hasil kegiatan pengabdian ini menunjukan bahwa para peserta sangat antusias dan menyimak selama proses berlangsung, hal ini dilihat dari peningkatan nilai pretest dari 61,53 menjadi 81,92 dan aktifnya peserta saat melakukan praktik. Praktik dan demonstrasi dimaksudkan untuk memberikan ketrampilan kepada para peserta dalam menentukan formula yang tepat dalam pembuatan sabun cuci dari mijel. Setelah kegiatan ini berlangsung diharapkan ibu-ibu akan semakin sadar dengan bahaya penggunaan mijel dan bisa memanfaatkan mijel menjadi produk yang lebih bermanfaat.
Pembuatan Kompos Seresah Menggunakan Inokulum Fungi Saprotrof Di Pesantren Darul Fattah Gedung Meneng Bandar Lampung Salman Farisi; Bambang Irawan; Suratman Suratman; Hendri Busman; Agung Abadi Kiswandono
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 3 No. 1 (2022)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v3i1.70

Abstract

Kompos adalah zat akhir suatu proses fermentasi tumpukan sampah/serasah tanaman termasuk bangkai binatang. Fermentasi tersebut perlu dipercepat dengan bantuan manusia. Isolat fungi saprotrof ini sangat potensial untuk dikembangkan menjadi inokulum pengomposan dalam pembuatan pupuk organik. Kompos yang diinduksi dengan inokulum fungi ini mampu menghasilkan pupuk organik dengan relatif cepat dan mengandung nutrisi tanaman yang tinggi. Prospek kebutuhan pupuk organik cukup tinggi dalam menunjang pertanian organik sehingga diperlukan penyebaran informasi IPTEK dan pelatihan kepada masyarakat tentang pembuatan pupuk organik dengan induksi inokulum fungi saprotrof. Selain itu ketersediaan seresah tanaman pekarangan melimpah dan terbebas dari residu bahan kimia. Hasil kegiatan ini memperlihatkan terjadi peningkatan pengetahuan dan pemahaman peserta pelatihan dalam pembuatan pupuk kompos dari seresah daun. Rata-rata peningkatan pengetahuan peserta pelatihan sebesar 30%.
AKTIVITAS DAN KARAKTERISASI ENZIM PROTEASE ISOLAT Bacillus sp. (UJ132) SECARA KUALITATIF DAN KUANTITATIF Sumardi Sumardi; Salman Farisi; Chiristina Nugroho Ekowati; Milsa Solva Diana
Jurnal Riset Akuakultur Vol 14, No 3 (2019): (September, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (679.012 KB) | DOI: 10.15578/jra.14.3.2019.193-199

Abstract

Bakteri penghasil enzim protease memiliki kemampuan untuk melakukan bioremediasi limbah protein. Penelitian ini dilakukan untuk mengetahui aktivitas dan karakterisasi enzim protease dari isolat Bacillus sp. (UJ132) yang diisolasi dari udang pasir (Metapenaeus affinis) di kawasan hutan mangrove Desa Margasari, Lampung Timur. Uji aktivitas enzim dilakukan secara kualitatif dan kuantitatif. Karakterisasi enzim meliputi penentuan suhu dan pH optimum, pengaruh ion logam, serta penentuan Km dan Vmaks. Dari hasil percobaan diketahui bahwa enzim protease dihasilkan pada waktu produksi optimum 18 jam dengan aktivitas protease sebesar 0,09 U/mL. Suhu optimum enzim ini yaitu pada suhu 50°C yang menghasilkan aktivitas sebesar 0,08 U/mL. Enzim protease ini mempunyai kondisi optimum pada pH 5 dengan nilai aktivitas 0,09 U/mL. Semua ion logam (Ca2+, Mn2+, Cu2+, Mg2+) berfungsi sebagai inhibitor kecuali ion Fe3+ yang berfungsi sebagai aktivator pada konsentrasi 1 mM dan 5 mM. EDTA dengan konsentrasi 1 mM dan 5 mM berfungsi sebagai inhibitor pada enzim protease isolat UJ132. Nilai Vmaks enzim protease 0,33 U/mL sedangkan Km senilai 4,59 mg/mL substrat, enzim ini mempunyai afinitas yang tinggi terhadap substrat.Shrimp farming produces protein wastes which mainly come from the remnants of given feed and excreta (feces) of shrimp. Bacteria known to possess protease enzymes have the ability to solve this protein waste problem in the shrimp farming industry. This study was conducted to determine the production and characterization of protease enzyme from Bacillus sp. (UJ132) isolated from the mangrove forest area of Margasari Village of Lampung Timur. The enzyme activity test was done qualitatively and quantitatively. The objectives of this study were to determine the optimum production of the enzymes and observe their characteristics, including determining the temperature and optimum pH, the effect of several metal ions, as well as Km and Vmax. The experimental results revealed that the protease enzyme had an optimum time of 18 hours of protease activity as much as 0.09 U/mL. The optimum temperature of this enzyme was 50°C which produced an activity of 0.08 U/mL. This protease enzyme has an optimum working condition at pH 5 with an activity value of 0.09 U/mL. All metal ions (Ca2+, Mn2+, Cu2+, Mg2+) acted as inhibitors except Fe3 + ions which acted as activators at concentrations of 1 mM and 5 mM. EDTA with a concentration of 1 mM and 5 mM served as an inhibitor of UJ132 isolate protease enzyme. The value of Vmax of the protease enzyme was 0.33 U/mL while Km was 4.59 mg/mL suggesting that this enzyme has a high affinity with the substrate.
Pelatihan pengolahan minyak jelantah menjadi sabun cuci untuk pemberdayaan ibu-ibu PKK di Bandar Lampung Kusuma Handayani; Mohamad Kanedi; Salman Farisi; Wawan Abdullah S
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.123-127

Abstract

Minyak jelantah (mijel) is known in Indonesia as waste cooking oil. Even though it is a waste, mijel is still used by consumers for savings. The problem is, if it is used repeatedly more than three times, mijel can cause health problems. In addition to health hazards, if discharged through water channels, it can cause environmental pollution. Lack of public knowledge about the dangers of using the mijel in the end will have a negative impact. To educate the public about the health-risks of using mijel, we held a short training for housewives in Kelurahan Tanjung Agung, Bandar Lampung on July 22, 2020. This training provided the targeted housewives with the knowledge and skills to process the waste cooking oil into laundry soap. The purpose of this activity was to educate and provide training to the community, especially PKK women who interact directly with the use of mijel, about the dangers of mijel and how to manage it. PKK women who participated in the training seemed interested and understood the explanation described, this can be seen from the increase in the average pretest score from 55.7 to 81.3 at posttest. The benefits obtained by the participants are: 1) Understanding that using mijel more than three times can cause health problems for consumers. 2) Processing of waste cooking oil to laundry soap can reduce pollution in the aquatic environment.3) Laundry soap made from waste cooking oil can be a source of family income.
Aktivitas Enzim Hidrolase pada Penapisan Isolat Actinomycetes Kandidat Probiotik Udang Sumardi Sumardi; Vanya Qatrunada; Salman Farisi; Achmad Arifiyanto; Christina Nugroho Ekowati
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.
Aktivitas Biosurfaktan Serratia Marcescens strain MBC1 dalam Mengemulsikan Solar dengan Variasi pH dan Media Cindy Lukyta Ratih Riyanto; Sumardi Sumardi; Salman Farisi; Christina Nugroho Ekowati; Achmad Arifiyanto
Jurnal Sumberdaya Alam dan Lingkungan Vol 8, No 3 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsal.2021.008.03.3

Abstract

ABSTRAK Resiko pencemaran lingkungan akibat tumpahan solar meningkat tiap tahunnya, oleh karena itu diperlukan upaya ramah lingkungan dengan biaya produksi rendah. Penelitian ini menggunakan bakteri Serratia marcescens strain MBC1 dengan tujuan menguji aktivitas biosurfaktan yang dihasilkan dalam melarutkan solar. Bakteri ini ditumbuhkan pada media produksi Trypton Water, limbah cair produksi tepung tapioka, dan limbah cair produksi  tepung maizena yang masing-masing telah diberi variasi pH yaitu 6, 7, dan 8 kemudian diinkubasi selama 7 hari. Biosurfaktan dari media produksi dipanen dengan sentrifuse dan diuji aktivitas biosurfaktan dengan 3 parameter uji yaitu uji drop collapse, uji oil displacement, dan uji emulsifikasi. Hasil penelitian menunjukkan bahwa biosurfaktan yang dihasilkan dari ketiga jenis media produksi mampu melarutkan solar. Biosurfaktan hasil produksi dari media limbah cair produksi tepung maizena pH 7 menunjukkan aktivitas melarutkan solar paling optimum dengan indeks emulsifikasi sebesar 63.88%.Kata kunci:  biosurfaktan, Serratia marcescens strain MBC1, solarABSTRACTThe risk of environmentall pollution due to diesel fuel spills increases every year therefore it takes an effort that is safe for the environment with low production costs. These bacteria are grown in the media productions that are Tryptone Water, cornstarch wastewater and tapioca wastewater each of them had been given pH variation that are 6, 7, and 8 then incubated for 7 days. Biosurfactants from media productions were harvested by centrifuge and tested for biosurfactant activity with 3 test parameter there are drop collapse test, oil displacement test, and emulsification test. The result showed that the biosurfactants produced from the three types of media production were able to dissolve diesel fuel. Biosurfactant produced from cornstarch wastewater pH 7 showed the most optimum dissolving of diesel fuel with emulsification index is 63.88%.Keywords:  biosurfactant, Serratia marcescens strain MBC1, diesel fuel
THE INHIBITION OF RED CHILIES RIPENING PROCESS AT FRESH WEIGHT AND TOTAL CARBOHYDRATE CONTENT BY RED LIGHT Martha Lulus Lande; Salman Farisi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.162

Abstract

The research was conducted in a factorial experiment with factor A: far-red light treatment with 2 levels: control (without far-red light), and treatment (far-red light). Factor B was a time of observation with 3 levels: 4, 6, 8 days after wrapping. Wrapping each detached fruit in a combination of four layers of blue-colored plastic, one layer of green-colored plastic, and two layers of red-colored plastic resulted in a red light treatment. Transmission measurement of that plastic combined with a spectrophotometer at wavelength 700-735 nm was 75%. To deter fresh weight, each fruit was weighted with an analytical balance. Total soluble carbohydrate content was determined by the phenol-sulfuric method and counted based on the glucose standard curve (mg/gram tissue). Analysis of variance was conducted at a 5% significant level, and if the interaction was significant, then continued with simple effect determination at a 5% significant level by the F test. The fresh weight average of the soluble carbohydrate content of treated fruits was higher than the control. The fresh weight of chili fruits was relatively constant during observation of both treated and controlled fruits. The total soluble carbohydrate content average relatively did not differ between treated and control fruits. Total soluble carbohydrate content was increased 6 days after treatment and decreased 8 days after treatment. The result of this research concluded that red light inhibited the ripening process of chili fruits by slowing the decrease of fresh weight.
MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.) Nadya Febri Harlifia; Bambang Irawan; Salman Farisi; Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.163

Abstract

Bamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.)
BROMELAIN WASTE TEA COMPOST INDUCED BY LIGNINOLYTIC INOCULUM OF Trichoderma sp. ON THE GROWTH OF LEAF NUMBER AND CHLOROPHYLL CONTENT OF CHILI (Capsicum annuum L.) Syafira Clarisa Huda; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.165

Abstract

Pineapple is one kind of fruit that contains a lot of nutrition.  The waste from residual pineapple processing is called bromelain.  Bromelain contains polymer complex in the form of organic sources from nature if it could be decomposed properly.  One way to exploited bromelain is by composting.  Composting is done with the help of fungi of Trichoderma sp. to speed up the decomposition process.  The resulting compost is aerated to increase the nutrients and form a further decomposition process in the soil.  The research was carried out from January till March 2021 in the Microbiology Laboratory Biology, Faculty of Mathematics and Science, University of Lampung.  The parameters of this research are the content of chlorophyll and the number of leaves.  This research used a completely Randomized Design with 7 treatments that are A1 (24 hours of mixed bromelain), A2 (48 hours of mixed bromelain), A3 (72 hours of mixed bromelain), A4 (24 hours of pure bromelain), A5 (48 hours of pure bromelain), A6 (72 hours of pure bromelain), and A7 (control).  Based on this research the value of the highest chlorophyll A on A6 treatment is 0,655.  A value of the highest on chlorophyll B on A6 treatment that 0,429.  The highest total chlorophyll on the A6 treatment is 1,048.  Based on this research the A4 treatment has the highest average number of leaves compared to other treatments.    
Co-Authors Achmad Arifiyanto Agung Abadi Kiswandono Agung Setia Ningsih Agustrina, Rochmah Aspita Laila Bambang Irawan Bella Noor Arfianty Chiristina Nugroho Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Cindy Lukyta Ratih Riyanto Emantis Rosa Endang Linirin Widiastuti Fadila Raisyadikara Hendri Busman Hendri Busman Jani Master Jihan Haura Komang Rima Kusuma Handayani M Kanedi Martha Lulus Lande Milsa Solva Diana Mohamad Kanedi Nadya Febri Harlifia Nuning Nurcahyani Primasari Pertiwi Qotrunnada Salsabila Rizka Oktavia Sumard Sumard Sumardi Sumardi, Sumardi Suratman Suratman Suratman Suratman Suratman Umar Sutyarso Syafira Clarisa Huda Tugiyono Vanya Qatrunada Wawan Abdullah S Wawan Abdullah Setiawan Yosi Dwi Saputra Yulianty Yulianty Yunita