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All Journal Jurnal Ilmu Lingkungan Jurnal Bioterdidik: Wahana Ekspresi Ilmiah BERITA BIOLOGI BIOMA : Jurnal Biologi Makassar Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Ilmiah Hijau Cendekia Jurnal Ilmiah Farmasi Farmasyifa Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Gunung Djati Conference Series JURNAL BIOLOGI PAPUA AMMA : Jurnal Pengabdian Masyarakat
Bambang Irawan
FMIPA Universitas Lampung
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The Effect of Coconut Water (Cocos nucifera L.) and Atonik to The Growth of Tomato Plant (Lycopersicum esculentum Mill.) Intan Poespita Windiyani; Tundjung T. Handayani; Zulkifli Zulkifli; Bambang Irawan
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.9

Abstract

The purpose of this study was to find how the effects of coconut water plus atonic on tomato plant growth. The study was conducted from October to November 2019 at the Laboratory of Botany, Department of Biology FMIPA University of Lampung. The study was conducted in a 2x3 factorial experiment with a completely randomized design. Factor A is young coconut water with 3 levels of concentration i.e. 0% v/v, 25% v/v, and 50% v/v. Factor B is atonic with 2 concentration levels, namely 0% v/v and 10% v/v. As variables are high, fresh weight, dry weight, relative water content, and chlorophyll content of plants. Levene Test, Variance Analysis is carried out at a 5% significance level. Main effects and simple effects were analyzed by the Tukey test at 5% significance level. The results showed that the interaction between coconut water and atonic had a negative effect on the growth of tomato plants. The results of the study concluded that coconut water combine with atonic can not improve tomato plant growth.
The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth Masniar Vina Kesti; Bambang Irawan; Priyambodo Priyambodo; Martha Lulus Lande
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.18

Abstract

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.
Effect of Drought-Stress Conditions in Chlorophyll Content of Dendrobium sp. Planlets Fesya Salma Putri; Endang Nurcahyani; Yulianty Yulianty; Bambang Irawan
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.26

Abstract

Dendrobium sp. was a popular ornamental plant and has high economic value. One of many difficulties in the growth of orchids in Indonesia is low humidity and lack of water availability. Regulatory Substance Growth in the form of an atonic solution can increase the growth of orchid plants. The purpose of this study was to determine the effect of atonic solutions on orchid chlorophyll content in drought stress conditions. This study used a 3x3 factorial design. Factor A is the atonic solution with 3 levels of concentration: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v and 25% b/v. The parameters tested were chlorophyll a, b and total Dendrobium orchid plantlets. The results showed that atonic solution and PEG 6000 significantly affected the chlorophyll a, b and total content of chlorophyll. The higher concentration of the atonic solution, the more content of chlorophyll a, b and total plantlets of decreased in drought stress conditions in vitro.
UJI EFEKTIFITAS EKSTRAK TUMBUHAN URANG ARING (Eclipta alba (L.) Hassk) TERHADAP PERTUMBUHAN JAMUR Colletotrichum sp. PENYEBAB PENYAKIT ANTRAKNOSA Billi Andreas; Christina Nugroho Ekowati; Yulianty Yulianty; Bambang Irawan
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 5 No. 1 (2018)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v5i1.62

Abstract

Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp Sumardi Sumardi; Rochmah Agustrina; Bambang Irawan; Indah Selfiana
Jurnal Ilmu Lingkungan Vol 16, No 2 (2018): Oktober 2018
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.614 KB) | DOI: 10.14710/jil.16.2.173-177

Abstract

ABSTRAKTujuan penelitian ini adalah mengetahui pengaruh paparan medan magnet 0.2 mT pada ion logam Fe dan Zn dalam media pertumbuhan terhadap produksi protease Bacillus sp.  Ion logam yang digunakan yaitu Fe dan Zn dalam bentuk garam FeCl3 dan ZnCl2. Hasil penelitian menunjukkan bahwa ion logam Fe (0.01% b/v) yang terpapar medan magnet   mempunyai nilai Indeks Proteolitik 3,36 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,74 dengan lama inkubasi 18 jam. Sedang ion logam Fe (0.01% b/v) yang tidak terpapar medan magnet mempunyai nilai Indeks Proteolitik 1,79 dengan lama inkubasi 10 jam, dan indeks proteolitik 2,12 dengan lama inkubasi 18 jam. Sedangkan pada ion logam Zn (0.005% b/v) yang terpapar medan magnet dan tidak terpapar medan magnet menyebabkan aktivitas Bacillus mati. Larutan ion logam Fe 0,01% dalam media cair menghasilkan produksi protease lebih baik (0,06 U/ml) dibandingkan dengan produksi protease tanpa dipemapar medan magnet (0,00 U/ml).Kata kunci: Bacillus sp., paparan medan magnet, indeks proteolitik.ABSTRACTThe purpose of this study was to determine the effect of metal ions Fe and Zn were exposed to a magnetic field of 0.2 mT to the production of proteases in Bacillus sp. The metal ions used are Fe and Zn in the form of FeCl3 and ZnCl2 salts. The results showed that the culture medium containing metal ions Fe (a 0.01% w/v) were exposed by the magnetic field has a value of proteolytic index of 3.36 with 10 hour long incubation, and proteolysis index of 2.74 with 18 hour incubation time. Culture medium containing metal ions Fe (a 0.01% w/v) which are not being exposed to the magnetic field has a value of proteolytic index of 1.79 with 10-hour long incubation, and proteolysis index of 2.12 with 18 hour incubation time. While the culture medium containing metal ions Zn (0.005% w/v) either exposed or not exposed to the magnetic field causes the activity of Bacillus sp die. The solution of 0.01% Fe metal ion in liquid medium resulted in better protease production (0.06 U/ml) than production of proteases without exposure to magnetic fields (0.00  U/ml)Keywords: Bacillus sp., magnetic field, proteolytic index, and proteolysis indexSitasi: Sumardi, Agustrina R., Irawan B., dan I. Selfiana. (2018). Pengaruh Paparan Medan Magnet 0,2 mT pada Ion Logam Fe dan Zn dalam media pertumbuhan terhadap produksi Protease Bacillus sp. Jurnal Ilmu Lingkungan, 16(2), 173-177,doi:10.14710/jil.16.2.173-177
PENGARUH PEMAPARAN MEDAN MAGNET 0,2 mT PADA MEDIA YANG MENGANDUNG LOGAM (Al, Pb, Cd, dan Cu) TERHADAP Bacillus sp DALAM MENGHASILKAN PROTEASE Sumardi Sumardi; Rochmah Agustrina; Bambang Irawan; Shofia Rodia
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3780

Abstract

This study aimed to determine the effect of 0.2 mT magnetic field exposure on metals (Al, Pb, Cd and Cu) containing media, to proteolytic index of Bacillus sp., protease activity and cell morphology of Bacillus sp. This study consisted of three stages. The first stage was a proteolytic test on solid media containing milk. The second stage was the production of protease enzymes in liquid media. The third stage was Scanning Electron Microscopy (SEM) analysis on a culture treatment known as protease enzyme activity. The results showed that the largest proteolytic index on CuCl2 metal ion content of magnetic 0.2 mT magnetic field for 10 minutes increased the value of the largest proteolytic index was 4.33. While in the ion solution containing CdCl2, Bacillus sp. culture did not grow. In the production of enzymes in the liquid medium, the highest enzyme activity (0.140 U/ ml) was produced on a medium containing AlCl3 and exposed to magnetic fields. The SEM analysis also proved that the supplementation of AlCl3 increased cell length by 2.38 and 2.78 times longer than control without magnetic field and for magnet field, respectivelly. 
Pembuatan Kompos Seresah Menggunakan Inokulum Fungi Saprotrof Di Pesantren Darul Fattah Gedung Meneng Bandar Lampung Salman Farisi; Bambang Irawan; Suratman Suratman; Hendri Busman; Agung Abadi Kiswandono
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 3 No. 1 (2022)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v3i1.70

Abstract

Kompos adalah zat akhir suatu proses fermentasi tumpukan sampah/serasah tanaman termasuk bangkai binatang. Fermentasi tersebut perlu dipercepat dengan bantuan manusia. Isolat fungi saprotrof ini sangat potensial untuk dikembangkan menjadi inokulum pengomposan dalam pembuatan pupuk organik. Kompos yang diinduksi dengan inokulum fungi ini mampu menghasilkan pupuk organik dengan relatif cepat dan mengandung nutrisi tanaman yang tinggi. Prospek kebutuhan pupuk organik cukup tinggi dalam menunjang pertanian organik sehingga diperlukan penyebaran informasi IPTEK dan pelatihan kepada masyarakat tentang pembuatan pupuk organik dengan induksi inokulum fungi saprotrof. Selain itu ketersediaan seresah tanaman pekarangan melimpah dan terbebas dari residu bahan kimia. Hasil kegiatan ini memperlihatkan terjadi peningkatan pengetahuan dan pemahaman peserta pelatihan dalam pembuatan pupuk kompos dari seresah daun. Rata-rata peningkatan pengetahuan peserta pelatihan sebesar 30%.
IMOBILISASI BAKTERI ASAM LAKTAT DENGAN MENGGUNAKAN ZEOLIT DAN NATRIUM ALGINAT Kinasih Cahyono; Sumardi Sumardi; Bambang Irawan; Sri Wahyuningsih; Endang Nurcahyani
Jurnal Ilmiah Farmasi Farmasyifa Vol 4, No 1 (2021)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jiff.v4i1.6372

Abstract

Lactobacillus sp merupakan salah satu spesies yang sering digunakan sebagai probiotik, namun kelemahan dari bakteri tersebut adalah tidak toleran terhadap  pH rendah (asam), di cairan empedu, serta pada suhu yang tinggi. Bakteri probiotik harus tetap hidup sejak mereka dikonsumsi hingga menetap di usus. Hal ini sulit karena bakteri harus melewati pH asam ekstrim di saluran pencernaan. Tujuan dalam penelitian ini adalah meningkatkan viabilitas BAL dalam kondisi simulasi asam lambung (pH=2) dan garam empedu (ox bile 0,5%) yang diimobiliasi dengan zeolite-natrium alginat. Enkapsulasi bakteri probiotik adalah alternatif yang memberikan perlindungan bagi sel-sel hidup yang berada pada kondisi yang merugikan. Berdasarkan hasil uraian di atas maka bertujuan untuk meningkatkan jumlah kelangsungan hidup bakteri Lactobacillus sp dalam kondisi simulasi asam lambung dan garam empedu bakteri probiotik dengan menggunakan imobilisasi natrium alginat-zeolit serta mengetahui gen penghasil antibiotik pada bakteri asam laktat. Metode yang digunakan dalam imobilisasi bakteri adalah ekstrusi. Berdasarkan hasil penelitian maka dapat disimpulkan bahwan Imobilisasi dengan penyalut natrium alginate-zeolit mampu meningkatkan viabilitas bakteri asam laktat. Dalam kondisi simulasi lambung dan garam empedu. Bakteri asam laktat (BAL) menghasilkan bakteriosin yang dapat berfungsi untuk menghambat bakteri Gram positif dan Gram negatif  berasal dari gen penghasil antimikroba yaitu terdapat gen plantaricin, casseicin, acidocin, lactacin B, helviticin Kata Kunci : Imobilisasi,  Natrium Alginat, Bakteri Asam Laktat, Zeolit, Gen Antimikroba
APLIKASI PUPUK ORGANIK CAIR DARI KULIT PISANG KEPOK (Musa paradisiaca L.) TERHADAP PERTUMBUHAN TANAMAN CABAI MERAH ( Capsicum annuum L.) Yulianty Yulianty; Rista Wahyu Mudya; Bambang Irawan; Martha Lulus Lande
Jurnal Ilmiah Hijau Cendekia Vol 7 No 1 (2022): Jurnal Ilmiah Hijau Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/hijau.v7i1.2256

Abstract

Tujuan dari penelitian ini yaitu untuk mengetahui efektivitas pengaruh pemberian pupuk organik cair dari kulit pisang terhadap pertumbuhan tanaman cabai dan konsentrasi yang terbaik yang dibutuhkan untuk pertumbuhan tanaman cabai. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 6 perlakuan dengan 4 ulangan yaitu P0 (kontrol), P1 (100ml), P2 (200ml), P3 (300ml), P4 (400ml), P5 (500ml). Variabel yang diamati yaitu (1) tinggi tanaman, (2) jumlah daun, (3) berat kering, (4) berat basah, dan (5) panjang akar. Data hasil pengamatan ini di homogenkan dengan uji Levene, kemudian dianalisis ragam (ANARA) pada taraf 5%, jika hasil signifikan dilanjutkan dengan uji Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil dari penelitian ini yaitu kulit pisang mampu memberikan pengaruh pada tinggi tanaman minggu ke 1 dan 2. tetapi tidak berbeda nyata terhadap jumlah daun, panjang akar, berat basah dan berat kering. Perlakuan P3 (300 ml) merupakan dosis yang terbaik dalam meningkatkan pertumbuhan tanaman cabai.
Pertumbuhan Planlet Anggrek Cattleya sp. Setelah Penambahan Ekstrak Tomat (Solanum lycopersicum L.) pada Medium Vacin and Went T. Indah Setia Ningsih; Endang Nurcahyani; Zulkifli Zulkifli; Bambang Irawan
AGRITROP Vol 19, No 2 (2021): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v19i2.6465

Abstract

Tujuan dari penelitian ini adalah mengetahui konsentrasi ekstrak tomat (Solanum lycopersicum L.) yang efektif bagi faktor-faktor pertumbuhan planlet Cattleya sp. yaitu persentase jumlah  planlet hidup, tinggi planlet, jumlah daun, jumlah tunas dan kandungan klorofil. Penelitian ini dilaksanakan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 1 faktor berupa ekstrak tomat dengan  menggunakan 5 taraf  konsentrasi ekstrak tomat yaitu 0 % (sebagai kontrol), 5% , 10%,15% dan 20%. Penelitian ini dilakukan dengan 5 kali ulangan pada setiap konsentrasi dan setiap ulangan terdiri dari 2 planlet tanaman anggrek Cattleya sp. Hasil penelitian menunjukkan bahwa pemberian konsentrasi ekstrak tomat pada medium Vacin and Went (VW) yang efektif untuk pertumbuhan planlet anggrek Cattleya sp. yaitu konsentrasi 15% (v/v) untuk peningkatan jumlah daun dan konsentrasi 5% (v/v) untuk peningkatan  jumlah tunas. Pemberian berbagai konsentrasi ekstrak tomat pada tinggi planlet serta kandungan klorofil a, b dan total memberikan efek yang  menghambat.
Co-Authors Agung Abadi Kiswandono Agustrina, Rochmah Aspita Laila Billi Andreas Christina N. Ekowati Christina Nugroho Ekowati Christina Nugroho Ekowati Ega F.S. Bina Emantis Rosa Endang Nurcahyani Fadila Raisyadikara Fesya Salma Putri Hendri Busman Herta M. Manullang Indah Selfiana Indriani Indriani Intan Poespita Windiyani Jani Master Jihan Haura Kinasih Cahyono Kusuma Handayani Mahfut Martha Lulus Lande Martha Lulus Lande Masniar Vina Kesti Moza Fierda Atiek Nabela H.N. Harfiani Nadya Febri Harlifia Priyambodo Priyambodo Rista Wahyu Mudya Salman Farisi Shofia Rodia Sri Wahyuningsih Sumardi, Sumardi Suratman Suratman Suratman Suratman Sutyarso Syafira Clarisa Huda T. Indah Setia Ningsih Tugiyono Tundjung T. Handayani Wanda Amelia Wawan Abdullah Setiawan Wuri Artikasari Yulianty Yulianty Yulianty Yulianty Zulkifli Zulkifli zulkifli zulkifli