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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 223 Documents
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The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth Masniar Vina Kesti; Bambang Irawan; Priyambodo Priyambodo; Martha Lulus Lande
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 1 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i1.18

Abstract

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.
Maya Index Analysis of Dengue Fever Vector in East Metro Sub-District Lampung Province Indonesia Betara Sona; Emantis Rosa; M. Kanedi; Tugiyono Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.20

Abstract

Dengue fever was one of the dangerous diseases due to it might cause death in a short time. It transmitted through its vectors, namely, Aedes aegypti and Aedes albopictus. Maya index is an indicator that used to identify a high-risk area or not as a breeding ground for Aedes sp. The purpose of this study was to determine the type of landfill which has the potential as a breeding ground for mosquitoes, to find out the larvae found in various landfill sites, and to know the Maya index status in Metro Timur Subdistrict which was carried out in November-December 2017. This research conducted by a direct survey of 100 residents' houses in East Metro. The results indicated that the type of controlled water reservoir that had the most potential as a breeding place for mosquitoes was a bathtub, while the type of water reservoir that was not controlled which had the potential as a place for mosquito breeding was a fish pond usage. There were 2 types of larvae found, namely, Aedes aegypti larvae with a percentage of 47.7% and Aedes albopictus larvae with a percentage of 52.3%. The Maya index status detected there, was a medium category, which obtained from the BRI combination and HRI category. Besides, it did not mean that the area has been free from dengue cases. The role of the community is still very much needed in eradicating landfills which has the potential as a breeding place for dengue mosquitoes vector.
Aggressiveness and Libido of Male Mus musculus L. in Ethanol Extract of Red Ginger (Zingiber officinale Roxb. var. Rubrum) Nida Nurhanifah; Sutyarso Sutyarso; M. Kanedi; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.21

Abstract

The residue of paraquat herbicides may cause human health problems. Paraquat causes the testosterone hormone to decline. Testosterone hormone affects aggressive and sexual behavior. It is caused by free radicals in the body. Free radicals can be non-reactive if in the body there are antioxidants. Red ginger could be used as an antioxidant because it has active phenolic compounds, such as arginine, gingerol, shogaol, zingerone, gingerdiol, and zingiber. Ginger could increase serum testosterone levels. The purpose of this research is to know the effect of red ginger ethanol extract on aggressiveness and libido of male mice induced by paraquat herbicide. This study used a Completely Randomized Design (RAL) consisting of six treatment groups with four replications each. Group P1 as control (aqua dest), P2 (paraquat at dose 20 mg/kg BM), P3, P4, P5 (paraquat with dose 20 mg/kg BM and red ginger extract at 200 mg/kg BM, 400 mg/kg BM, 600 mg/kg BM) and P6 (red ginger extract at 600mg/kg BM). Paraquat is given 2 times a week for 21 days and the red ginger extract is given daily for 35 days. The result of analysis with One-way ANOVA and continued BNT at 5% showed that giving of red ginger ethanol extract can increase aggressive behavior and libido of male mice induced paraquat herbicide.
Boosting Sperm Count Using Red Ginger in Mice Induced by Paraquat Dichloride (1.1-dimethyl-4.4 bipyridinium) Siti Umairoh; Sutyarso Sutyarso; M. Kanedi; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.22

Abstract

Paraquat dichloride (1.1'-dimethyl-4.4'-bipyridinium dichloride) was commonly herbicide used by most farmers in Indonesia. It raises herbicide-free radicals and affects male reproductive organs. It caused infertility such as a decrease in the number of spermatogenic cells. Fortunately, red ginger has capable of dealing with infertility problems. This study aims to see whether the ethanol extract of red ginger can increase the number of spermatocyte and spermatid cells induced by paraquat dichloride. This study uses a completely randomized design with 24 male mice were divided into six treatment groups with four replications. Group K as a control (H2O), J (Ginger 18 mg / ml), Pq (Paraquat 20mg / BB), P1 (Pq 20 mg / BB + EEJM 6 mg / ml), P2 (Paraquat 20mg / BB + EEJM 12 mg / ml) and P3 (Paraquat 20mg / BB + EEJM 18 mg / ml). Paraquat is given 2 times a week for 21 days, while the ethanol extract of red ginger added for 35 days. The number of cells spermatocyte and spermatid cells of male mice and performed analysis of variance (ANOVA) and Least Significant Difference Test (BNT) level of 5% observed as the parameter. The results showed that ethanol extract of red ginger 6, 12 and 18 mg/ml increased the number of spermatocyte and spermatid cells of male mice induced by paraquat dichloride.
Stomata Index of Cattleya sp. Lindl., Planlet in Drought-Stress Conditions Tara Sesafia Paletri; Endang Nurcahyani; Yulianty Yulianty; Rochmah Agustrina
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.23

Abstract

Cattleya was one of the most popular orchids by Indonesian. Constraints in cultivating Cattleya included the lack of water availability in an area, causing drought stress. Stress in drought in vitro can be simulated by adding Poly Ethylene Glycol 6000 and growth regulators in atonic form. The purpose was to determine the effect of atonic solution and PEG 6000 on the stomata index toward in vitro drought stress conditions. This study was carried out in a 3 x 3 factorial experiment. Factor A was an atonic solution with 3 concentration levels: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v, 25% b/v. The results showed that the higher the concentration of PEG 6000 and atonic concentrations caused a decrease in the stomata index of Cattleya orchid plantlets under drought stress conditions in vitro.
Organ-Protective Effect of Red Ginger Extracts in Male Mice Induced by Paraquat Kamiliah Tsany; Sutyarso Sutyarso; M. Kanedi; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.24

Abstract

Paraquat is a toxic compound that disrupts biochemical processes in the body, cell death, and multi-organ failure. The liver and lung are the target organ of paraquat toxicity. The body needs antioxidants to counteract free radicals. Red ginger (Zingiber officinale Roxb. Var rubrum) is a spice that contains antioxidants. This study was conducted to determine the effect of ethanol extract of red ginger (Zingiber officinale Roxb. Var rubrum) on liver and lung histology male mice induced by paraquat. The research used completely randomized design and use of 6 treatment groups, i.e. control group (given distilled water and standard feed), PQ group (induced by paraquat 20 mg/kg per BW), group P1 (given paraquat and ethanol extracts of red ginger 200 mg/kg per BW), group P2 (given paraquat and ethanol extract of red ginger 400 mg/kg per BW), group P3 (given paraquat and ethanol extract of red ginger 600 mg/kg per BW), group P4 (given ethanol extract of red ginger extract 600 mg/kg per BW). The data obtained will be tested using One Way ANOVA and continued by Least Significant Difference (LSD) with a 95% confidence level. The results showed ethanol extract of red ginger effective against liver and lung histology male mice induced by paraquat. The results also showed ethanol extract of red ginger with a dose of 400 mg/kg per BW is effective in protecting the liver and lung from damage caused induced by paraquat.
Nepenthes mirabilis (Lour.) Druce Planlet at a Various Levels of Murashige & Skoog Medium Density In Vitro Nadia Fakhriyati Arfa; Endang Nurcahyani; Zulkifli Zulkifli; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 2 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i2.25

Abstract

This study aims to determine the variation of the stomata index of the Kantong Semar (Nepenthes mirabilis) planlet at a various medium density of the Murashige and Skoog. This study used a Completely Randomized Design using one factor (medium density of the Murashige and Skoog). We used 5 levels of medium density, i.e.: 1/16 MS, 1/8 MS, 1/4 MS, 1/2 MS, and MS. Homogeneity test used Levene’s test of 5% significance level, then analysis of variance is carried out at 5% significance level and followed by Tukey test at 5% significance level. The results showed that the lower the level of Murashige and Skoog medium density on the Nepenthes mirabilis plantlet, the stomata index also increased.
Effect of Drought-Stress Conditions in Chlorophyll Content of Dendrobium sp. Planlets Fesya Salma Putri; Endang Nurcahyani; Yulianty Yulianty; Bambang Irawan
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.26

Abstract

Dendrobium sp. was a popular ornamental plant and has high economic value. One of many difficulties in the growth of orchids in Indonesia is low humidity and lack of water availability. Regulatory Substance Growth in the form of an atonic solution can increase the growth of orchid plants. The purpose of this study was to determine the effect of atonic solutions on orchid chlorophyll content in drought stress conditions. This study used a 3x3 factorial design. Factor A is the atonic solution with 3 levels of concentration: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v and 25% b/v. The parameters tested were chlorophyll a, b and total Dendrobium orchid plantlets. The results showed that atonic solution and PEG 6000 significantly affected the chlorophyll a, b and total content of chlorophyll. The higher concentration of the atonic solution, the more content of chlorophyll a, b and total plantlets of decreased in drought stress conditions in vitro.
Spinal Structure of Mice Fetus (Mus musculus L.) Treated by Acanthus ilicifolius L. Leaves Extract Tunggul Van Roy; Nuning Nurcahyani; Sutyarso Sutyarso; Hendri Busman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.27

Abstract

Jeruju had various chemical components. This study was conducted on the teratogenic test of jeruju leaf extract to determine abnormalities in fetal mice (Mus musculus L.). This study used a completely randomized design (CRD) method with 20 pregnant female mice divided into 4 groups: 1 group as a control and 3 groups treated with a dose of 3.75 mg/30grBB, 7.5 mg/30grBB, 15 mg/grBB, each with jeruju leaf extract consists of 5 repetitions. The results showed that the doses of 7.5 mg/30grBB and 15 mg/grBB significantly decreased fetal weight and length when compared with controls. This is suspected because there is an active substance in the jeruju leaf extract which is cytotoxic. Anatomically the administration of jeruju leaf extract does not cause abnormalities in the structure of the fetal spine of mice. This is due to the growing fetus taking priority from the many types of nutrients in the mother fluid so that the fetal bone can continue to grow and develop.
Gibberellic Acid (GA3) and Potassium Nitrate (KNO3): The Influence and Interaction inThe Senescence Process in White Rosa Sp. Nur Jannah Cortesa; Martha Lulus Lande; Zulkifli Zulkifli; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 6 No. 1 (2019)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v6i1.28

Abstract

The purpose of this study was to find out whether the combination of KNO3 and GA3 solutions were more effective than a single solution of KNO3 or GA3 in keeping the freshness of cut flowers. The research was conducted in Botanical Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences University of Lampung from November to December 2017. The experiment was conducted in 2 x 3 factorial experiment with factor A is GA3 solution with 3 concentration level: 0% w/v, 25% w/v, and 0.5% w/v. Factor B is KNO3 solution with 2 concentration levels: 0% w/v and 5% w/v. The parameters of this study were fresh weight, dry weight, relative water content, total chlorophyll content, total chlorophyll of leaves, and total carbohydrate content of rose cut flowers. Measurements were made 7 days after the immersion of cut flowers in KNO3 or GA3 solution, and mixed both. Homogeneity of variance and analysis of variance is determined at confidence level 5%. KNO3 simple effect at every level of GA3 concentration is determined by the F test at 5%. The results showed that there was no interaction between GA3 and KNO3 to fresh weight, dry weight, relative water content and chlorophyll content a, b and total white roses, but KNO3 reduced fresh weight of cut flowers by 48.15% and dry weight 58.60%. KNO3 increased the relative water content of white rose flowers by 6.85%. The results of the study concluded that the combination of GA3 and KNO3 is not effective to keep the freshness of white rose flowers cut.

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