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Advances in Tropical Biodiversity and Environmental Sciences

ATBES
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Published by Universitas Udayana

ISSN : 25496980 EISSN : 26220628 DOI : https://doi.org/10.24843/ATBES.2022.v06.i01

Core Subject : Health, Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Veterinary

The journal encompasses original research articles, review articles and case studies including: Environmental sciences, Biology, Atmospheric sciences, Environmental chemistry, Earth science, Ecology, Forestry, Agro-forestry, Biodiversity, Taxonomy, Ethnobiology, Vegetation Survey, Bioremediation, Geosciences, Organism and Conservation of Natural sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 100 Documents

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Characteristics of Beach Typology for the Development of Marine Tourism in the Blue Lagoon Beach of Karangasem Bali Suprabadevi Ayumayasari Saraswati; I Ketut Wija Negara; Dewa Ayu Angga Pebriani
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 2 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

Sygnathidae corals are included in the vurnarable category of CITES because the number in their natural habitat is decreasing. This is partly due to the high predatory activity and utilization as raw materials for traditional medicine so that it has high economic value. This research aims to study the ecological conditions including the distribution and abundance of Sygnathidae reef fish in the waters of Blue Lagoon Karangasem and to study the ecological conditions of the waters. The main marine tourism and recreation activities at Blue Lagoon Beach Karangasem, Bali are now snorkeling tours. This requires attention to the preservation of coastal ecosystems, because tourism is an industry that is very sensitive to external changes, so the region is of concern. Besides studies on marine tourism activities, scientific studies on tourism carrying capacity are also needed to determine the maximum number of tourist visitors that can still be tolerated by an ecotourism area. This research was conducted in the Blue Lagoon Coast Karangasem Bali. This study uses primary data analysis of water quality analysis, the method of determining the suitability of the region based on the multiplication of scores and weights obtained from each water brightness parameter category S1 with a score of 15, coral community cover category S3 with a score of 5, coral life form category S1 with a score of 9 , reef fish category TS with a score of 0, current speed category S2 with a score of 2, and depth of the category S3 with a score of 1. This study was conducted for 3 months, namely in June-August. The determination of the research station is based on the Purposive sampling technique. Secondary socioeconomic data obtained by questionnaire, interview / interview. The purpose of this study is to examine the suitability of the area as a marine tourism activity and analyze the carrying capacity (carrying capacity) of marine tourism areas.

Total Genomic DNA Extraction Studies from Seaweeds Wildan Mujahidul Basyar; Made Pharmawati; Ida Ayu Astarini
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 1 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

One of marine resources that has high value is seaweed. Seaweed is a carrageenan producer used in the food industry. Seaweed contains many minerals, vitamins and proteins that are useful for health. Carotenoids are pigments found in seaweed that function as antioxidants. The genes involved in carotenoid biosynthesis have been studied and provide opportunities for genetic improvement of seaweed. DNA is a basic requirement in molecular analysis. Therefore, a suitable method of DNA extraction from seaweed is needed. The aim of this research was to investigate DNA extraction method from several seaweed species and test the DNA quality through PCR-RAPD. Seaweed samples were collected from Pantai Bumi Perkemahan Taman Nasional Bali Barat and DNA was extracted using Doyle and Doyle’s method with modifications. PCR-RAPD was conducted using primer UBC127 and OPD 11 to test the quality of DNA. Results showed that 3 hours incubation in 60ºC had the best result of DNA extraction. However, the quality of DNA was low, as indicated by inconsistent PCR-RAPD products. Further optimization in DNA extraction is needed to obtain high quality DNA for genetic analysis.

Determination of Flavonoid, Tannin and Vitamin C Content from Methanol Extract Wrapping Stone Banana (Musa brachycarpa), Ketip Banana (Musa Paradisiaca Forma Typiaca) and Kepok Banana (Musa acuminata) Ni Putu Adriani Astiti; Dwi Ariani Yulihastuti
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 2 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Research has been done to investigate the levels of flavanoid, tannin, and vitamin C of methanol extract of Stone banana (Musa brachycarpa), Ketip banana (Musa paradisiaca forma typiaca ), and Kepok banana (Musa acuminata). Jukut Ares is a Balinese traditional cuisine frequently served in any events of celebrations. The use of young stems of such banana varieties as the main raw materials in the process of making jukut ares has not been scientifically elucidated. Antioxidants are compounds with ability to inhibit oxidation-related reactions due to free radicals that cause disturbance of saturated fatty acids of cell membrane, blood vessels, DNA, as well as adipose tissues which lead to occurrence of various diseases. Plants components will have antioxidant activities if they contain compounds (phenol and flavanoid) with ability to scavenge free radicals. Tannin is a water based phenolic compound and vitamin C is also a water based vitamin that play important roles in preventing us from various diseases. Extraction was conducted by applying maceration method and to investigate the levels of flavanoid, spectrophotometric UV vis with aluminum chloride (AlCl3) was applied. The level of vitamin C of the materials was conducted by Iodine titration method, while the level of tannin was determind by applying spectrophotometri. The results showed that methanol extract of Ketip banana stem contained the highest level of flavanoid with a quantity of 53.13 mg/100gr QE, and this was followed by stone banana (36.28) and kepok banana (32.07) The highest vitamin C level was indicated in the extract of ketip banana (418.32 mg/100gr), which is followed by kapok banana and stone banana. In the calculation of tannin content, pisang ketip showed the highest level, while pisang batu contained the lowest level of tannin.

Morphological and Anatomical Changes by Cochicine in Seedling of Impatiens balsamina L. Ni Made Sastriyani Wiendra; Made Pharmawati
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 2 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Impatiens balsamina L. is a plant that is widely cultivated in Bali. The flower of this plant is used as a component of offering in Hindu religious ceremonies. The flower petals are thin and wilt easily, therefore genetic modification is needed to get better quality. This research aimed to study the effect of 0.01% colchicine with soaking periods of 4 hours, 8 hours, 12 hours and 24 hours to seedlings of I. balsamina. Seeds of I. balsamina were germinated and sprouted seeds were treated with 0.01% colchicine for 4 hours, 8 hours, 12 hours and 24 hours. The sprouted seeds were then transfer to polybag with soil media. The experiment was designed with randomized blok design with five replicates. Observations were done on plant height, length and width of leaf, stem diameter, number of stomata as well as observation on plant stem cortex cells. Results showed that colchicine reduced plant height at three weeks after planting, while plant stem diameter increased. Control plants had the smallest leaf length and width. The number of stomata decreased at plants derived from colchicine treated sprouted seeds and the lowest stomatal number observed was at soaking duration of 24 hours.

Comparison of DNA Yield from Different Plant Materials of Plumeria sp. (Apocynaceae) Vanesa Martida; Made Pharmawati
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

DNA extraction that gives good quantity and quality DNA is a basic step that must be completed for molecular studies, especially in DNA fingerprint imaging. The aim of this research was to find out the better quality and quantity of DNA extracted from different plant materials of frangipani cultivars (Plumeria sp.). Leaves and flowers were collected from Taman Jepun, Denpasar Bali. Fresh young leaves and flowers were used as plant materials as well as dried leaves (silica gel dried leaves) of Plumeria sp. This research used CTAB buffer with modification as lysis buffer. Purification techique used NucleoSpin® Gel and PCR Clean Up Kit. The results showed that the colour of DNA solution from fresh material was clear and the quantities of DNA from young fresh leaves were between 70-300 ng/µl. The DNA colour solution from flowers was also transparent with concentration between 0-40 ng/µl. DNA isolated from dry material resulted in brown solution with DNA quantity between 30-100 ng/µl and need to be purified to obtain clear DNA solution.

Analysis of Plankton Abundance and Status of Water Quality Fish Cultivation “Sawah Tambak” with Overtime System in Kalitengah Subdistrict, Lamongan Regency Agung Pamuji Rahayu; Moch Saad; Norma Aprilia Fanni
Advances in Tropical Biodiversity and Environmental Sciences Vol 5 No 1 (2021): ATBES
Publisher : Institute for Research and Community Services Udayana University

The fish cultivation system in several villages in Kalitengah District uses an overtime system, namely a fish cultivation system that is carried out within 1 year (12 months) without passing rice planting. The research objective was to determine the abundance of plankton and the status of water quality fish cultivation “sawah tambak” with overtime system in Kalitengah District Lamongan. This is a quantitative research using descriptive methods. Based on the plankton abundance value at Station 1,2 and 3, the waters in the overtime system of ponds are in the Eutrooph category, with the abundance value > 12,000 Ind / L. Meanwhile station 4,5, and 6 are included in the Oligotrooph category since its abundance <10,000 Ind / L. The diversity value is within the range of 0.714267-1.823 which include in the category of small diversity and low community stability because the value of 0 <H '<1.5. All stations are in a moderate level of pollution which the diversity index value is within the range of 1 <H '<3. The Dominance Index (D) is in the range 0.212-1.224, Station 1 has a high dominance value because of the dominant species, namely Batrachospermum. Water quality parameters at all stations such asthe temperature ranges between 28.8-34.2 0C; the pH 8.5-9.2; Dissolved oxygen 4-10.44 mg / l; ammonia 0.009-0.17 mg / l; phosphate ranges from 0.025-2.3 mg / l. From the measurement results, water quality parameters show a good value for the growth of aquatic organisms.

Cover, Editors, and Table of Content of ATBES Vol.1 No.2 Sept 2017 Iriani Setyawati
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 2 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.397 KB)

Advances in Tropical Biodiversity and Environmental Sciences (ATBES) is a scientific journal published since 2017. The journal consistently publishes twice a year (Pebruary and September). The journal is registered in DOAJ. ATBES is employing a Cross Check Ref. to prevent any suspected plagiarism in the manuscripts.

Antibiotics Resistance Level of Vibrio spp. Isolated From Northern Bali Area Widya Purnama Dewi; Pande Gde Sasmita Julyantoro; I Wayan Darya Kartika
Advances in Tropical Biodiversity and Environmental Sciences Vol 4 No 2 (2020): ATBES
Publisher : Institute for Research and Community Services Udayana University

One conventional method that usually done when the organism infected by pathogenic bacteria is using antibiotics, either with single or combination usage. However, the misuse of antibiotics dosages leads to resistance development of pathogenic bacteria. This study aims to determine the antibiotic resistance level of Vibrio spp. which was isolated from the waters of North Bali and to investigate the difference of resistance level between Vibrio spp. isolated from the cultivation area and outside the cultivation area. This research was conducted at the Microbiology Laboratory of BKIPM Denpasar and the Laboratory of Fisheries at the Faculty of Marine Science and Fisheries, Udayana University from November 2019 to February 2020. Antibiotic tests were carried out in vitro using 8 types of antibiotics namely tetracycline, oxytetracycline, enrofloxacin, ciprofloxacin, amoxycillin, doxycycline, ampicillin, and erythromycin with different concentrations of 0.5, 1, 2, 4, 8, 16, 32, 64, 64, 128, 256 and 512 ppm. and this test was carried out using a microplate reader to obtain absorbance values before and after incubation to determine the level of resistance of isolated Vibrios. The research showed that overall minimum inhibitory concentration (MIC) of Vibrio was below 100 ppm while the Vibrio spp. isolated from cultivation area have higher resistance level compared to outside cultivation area.

Gastric Histopathology of White Rats After Administration of Kedondong Leaves Extract I Wayan Sudira; I Made Merdana; Ketut Budiasa
Advances in Tropical Biodiversity and Environmental Sciences Vol 2 No 1 (2018): ATBES
Publisher : Institute for Research and Community Services Udayana University

This study aims to determine the effect of ethanol extract of Kedondong leaves (Spondias dulcis G. Forst) on the gastropathological histology of white rats (Mus musculus). A total of 25 healthy male white rats with weights 200-250 g were randomly divided into five groups. A control group was only given a placebo. The treatment group of kedondong leaf ethanol extract sequentially with a dose of 125, 250, 500 and 1,000 mg/kg/day for 21 days orally. On the 22nd day all of the animals were sacrificed for its the gastric, followed by processing for histopathological preparations with Hematoxylin Eosin (HE) staining. The results of the study in all treatment groups were found gastric histology changes in the form of mild inflammatory cell infiltration, mild fatty degeneration and mild necrosis. The number of mice that experienced histopathological changes differed in each group. It can be concluded that the oral administration of kedondong leaf ethanol extract with a range at a dose of 125-1000 mg/kg/d for 21 days does not cause significant histopathological structure disturbance in the stomach of male white rats.

Utilization of Betel Leaf Extract as Botanical Pesticides to Control meloidogyne spp. and Tomato Plant Production Made Sritamin; I Dewa Putu Singarsa
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 1 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Meloidogyne spp. Are among the polyphagous pest that has spread around the world and has been reported to attack cultivated plants with economic values. Various ways had been applied to control the Meloidogyne spp. nematodes but has not yet showed any effective results. We are currently using synthetic pesticides because of its fast response and ability to maintain plant productions. The improper application of synthetic nematicides would have a negative impact on the environment. The aim of this research is to know the effectiveness of betel leaf concentration (Piper betel L.) to curb the development of nematodes on Root-Knot Meloidogyne spp and the growth of tomato plants. This is a descriptive research, with the utilization of betel leaf extract (Piper betel L,) by concentration treatment of 0 %, 5 %, 10 %, 15 % and 20%, each of which are repeated five times. Complete Random Sampling (CRS) with the F test is used and continued with the Duncan test at a 5% rate. The result shows that the betel leaf extract of the 20% concentration was the most effective either on the growth of plants and to the root-knot nematodes. The suppression of growth in the ground was 80,06% and the lowest was recorded with the 5% concentration ( 2,32 %); the suppression of root-knot was 45,45 % while the lowest was with the 5% concentration (12,12 %); the suppression of nematodes population in the roots was 45,45% while the lowest was with the 5% concentration (39,76 %); and last was the suppression of egg mass which was 61,73 % while the lowest was shown in the 5% concentration (18,32 %). It can be concluded in this research that the most effective betel leaf extract concentration was at 20%.Keywords: Meloidogyne spp., Piper betel L., CRS

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I Made Merdana

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+6281236118996

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Jl. Kampus Unud Bukit Jimbaran, Badung - Bali, 80361

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Kota denpasar,

Bali

INDONESIA